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41. 发明授权

US5770400A Exogenous gene expression vector containing chick .beta.-actin gene promoter 失效
公开(公告)号：US5770400A
公开(公告)日：1998-06-23
申请号：US814468
申请日：1997-03-10
申请人： Junichi Miyazaki , Ken-ichi Yamamura , Masatake Araki , Hiroshi Yonemura , Chikateru Nozaki
发明人： Junichi Miyazaki , Ken-ichi Yamamura , Masatake Araki , Hiroshi Yonemura , Chikateru Nozaki
IPC分类号： C07K14/02 , C12N9/38 , C12N15/85 , C12N15/70 , C12P21/02
CPC分类号： C07K14/005 , C12N15/85 , C12N9/2471 , C12Y302/01023 , C12N2730/10122 , C12N2800/108 , C12N2830/00 , C12N2830/005 , C12N2830/15 , C12N2830/60 , C12N2830/90 , C12N2840/44
摘要： An expression vector for the expression of an exogenous gene in an animal cell, which contains a chick .beta.-actin gene promoter and a restriction enzyme site for incorporating an exogenous gene at the downstream of said promoter and a process for expressing an exogenous gene, which comprises incorporating said exogenous gene into the expression vector at the restriction enzyme site for incorporating the exogenous gene, introducing said vector into an animal cell and culturing the obtained transformed animal cell. The expression system of the present invention can be applied to an expression of any exogenous gene and has an extremely high expression efficiency and is applicable to a wide range of host cells, and hence can sufficiently be utilized for industrial-scale production of a useful material.

42. 发明授权

US4997767A Yeast shuttle vector 失效
标题翻译：酵母穿梭载体
公开(公告)号：US4997767A
公开(公告)日：1991-03-05
申请号：US946329
申请日：1986-12-23
申请人： Chikateru Nozaki , Fukusaburo Hamada , Nobuya Ohtomo
发明人： Chikateru Nozaki , Fukusaburo Hamada , Nobuya Ohtomo
IPC分类号： C12N15/09 , A61K39/29 , C12N15/00 , C12N15/64 , C12N15/70 , C12N15/81 , C12R1/19 , C12R1/865
CPC分类号： C12N15/81 , C12N15/64 , C12N15/70 , Y10S435/911 , Y10S435/94 , Y10S435/942
摘要： Shuttle vectors including a DNA sequence of the yeast Saccharomyces cerevisiae including ars 1.2 micron ori and a marker gene for transformed yeast permitting synthesis of leucine by the transformant; adjacent to said ars 1 a DNA sequence of Escherichia coli which is either in EcoR1--PvuII or EcoR1--TthIII--I fragment of plasmid pBR322; and adjacent to the yeast marker gene the expression control region of the repressible acid phosphatase gene of yeast.
摘要翻译：穿梭载体包括酵母酿酒酵母的DNA序列，包括1.2微米的穗，以及转化酵母允许转化体合成亮氨酸的标记基因; 与所述ars1相邻的是质粒pBR322的EcoRⅠ-PvuII或EcoR1-TthIII-I片段中的大肠杆菌的DNA序列; 并与酵母标记基因相邻，是酵母抑制性酸性磷酸酶基因的表达控制区。

43. 发明授权

US08883165B2 Modified peptide vaccine derived from influenza M2 有权
标题翻译：来自流感M2的改良肽疫苗
公开(公告)号：US08883165B2
公开(公告)日：2014-11-11
申请号：US13392994
申请日：2010-08-23
申请人： Kazuyoshi Kaminaka , Chikateru Nozaki , Junichi Matsuda , Kiyoto Nishiyama
发明人： Kazuyoshi Kaminaka , Chikateru Nozaki , Junichi Matsuda , Kiyoto Nishiyama
IPC分类号： A61K39/12 , A61K39/00 , A61K39/385 , A61K39/145 , A61K38/00 , C07K17/00 , C07K14/005
CPC分类号： A61K39/145 , A61K39/00 , A61K39/12 , A61K2039/55505 , C07K14/005 , C12N2760/16122 , C12N2760/16134
摘要： A modified peptide derived from matrix protein 2 (hereinafter also referred to as “M2”), one of surface layer proteins of influenza virus, and a method for utilization of the modified peptide are provided. A peptide (hereinafter also referred to as “M2eC peptide”) that is made up by inserting cysteine residue(s) into a peptide (hereinafter also referred to as “M2e”) consisting of 23 amino acid residues of from positions No. 2 to No. 24 of M2 in influenza virus type A, a fusion protein consisting of said modified peptide and a polypeptide, an influenza vaccine comprising said modified peptide or said fusion protein as an active ingredient, a device which can be delivered into the body comprising said influenza vaccine, a nucleic acid fragment consisting of a nucleotide sequence encoding the amino acid sequence of said modified peptide or said fusion protein, an expression vector in which said nucleic acid fragment is incorporated, a host in which said expression vector is introduced, and an antibody that has a protective effect against influenza virus.
摘要翻译：提供了衍生自基质蛋白2的修饰肽（以下也称为“M2”），流感病毒的表层蛋白之一和使用修饰肽的方法。通过将半胱氨酸残基插入由位置2〜23的23个氨基酸残基组成的肽（以下也称为“M2e”）而构成的肽（以下也称为“M2eC肽” 流感病毒A型M2的第24号，由所述修饰肽和多肽组成的融合蛋白，包含所述修饰肽或所述融合蛋白作为活性成分的流感疫苗，可以递送到身体的装置，包括所述流感疫苗，由编码所述修饰肽或所述融合蛋白质的氨基酸序列的核苷酸序列组成的核酸片段，其中加入所述核酸片段的表达载体，导入所述表达载体的宿主和对流感病毒具有保护作用的抗体。

44. 发明授权

US08623374B2 Method for enhancing immune response with peptide 有权
标题翻译：用肽增强免疫应答的方法
公开(公告)号：US08623374B2
公开(公告)日：2014-01-07
申请号：US13220452
申请日：2011-08-29
申请人： Junichi Matsuda , Kazuyoshi Kaminaka , Chikateru Nozaki
发明人： Junichi Matsuda , Kazuyoshi Kaminaka , Chikateru Nozaki
IPC分类号： A61K38/17 , A61K39/00 , A61P37/04 , A61P25/00 , C07K14/435
CPC分类号： A61K39/0007 , A61K31/711 , C07K14/4711
摘要： An object of the present invention is to provide a safe and effective method for enhancing an immune response and a medicament for preventing or treating Alzheimer disease comprising amyloid β peptide that induces an enhanced immune response. An amyloid β peptide or a portion thereof with addition or insertion of cysteine and a method for enhancing an immune response using the peptide or a method for enhancing an immune response using the peptide together with an adjuvant. A medicament for preventing or treating Alzheimer disease comprising an amyloid β peptide or a portion thereof that induces an enhanced immune response. A DNA vaccine, that may have the same effect, comprising the gene encoding an amyloid β peptide or a portion thereof that induces an enhanced immune response with addition or insertion of cysteine.
摘要翻译：本发明的目的是提供一种用于增强免疫应答的安全有效的方法和用于预防或治疗阿尔茨海默病的药物，其包含诱导增强的免疫应答的淀粉样蛋白β肽。具有添加或插入半胱氨酸的淀粉样蛋白β肽或其部分，以及使用肽增强免疫应答的方法或使用肽与佐剂一起增强免疫应答的方法。一种用于预防或治疗阿尔茨海默病的药物，其包含诱导增强的免疫应答的淀粉样蛋白β肽或其部分。可以具有相同效果的DNA疫苗，其包含编码淀粉样蛋白β肽或其部分的基因，其通过加入或插入半胱氨酸诱导增强的免疫应答。

45. 发明授权

US08512709B2 Modified amyloid β peptide 有权
标题翻译：改良淀粉样β肽
公开(公告)号：US08512709B2
公开(公告)日：2013-08-20
申请号：US13124260
申请日：2009-10-16
申请人： Junichi Matsuda , Kazuyoshi Kaminaka , Chikateru Nozaki
发明人： Junichi Matsuda , Kazuyoshi Kaminaka , Chikateru Nozaki
IPC分类号： A61K38/17 , A61K39/00 , A61P25/00 , A61P37/04 , C07K14/435
CPC分类号： C07K14/4711 , A61K39/0007 , A61K2039/53
摘要： An object of the invention is to provide a peptide based on a sequence of an amyloid β peptide that may allow for induction of enhanced immune response and is safe and efficacious for prophylaxis and treatment of Alzheimer disease. An amyloid β peptide or a portion thereof with addition or insertion of cysteine or a cysteine analogue, and a method for enhancing immune response to amyloid β using said peptide, a medicament for prophylaxis and treatment of Alzheimer disease using said amyloid β peptide that induces an enhanced immune response, and a DNA vaccine comprising a gene coding for an amyloid β peptide or a sequence derived from an amyloid β peptide with addition or insertion of cysteine or a cysteine analogue, as expected to be similarly efficacious.
摘要翻译：本发明的目的是提供一种基于淀粉样蛋白β肽序列的肽，其可以诱导增强的免疫应答，并且对于阿尔茨海默病的预防和治疗是安全有效的。具有添加或插入半胱氨酸或半胱氨酸类似物的淀粉状蛋白β肽或其部分，以及使用所述肽增强对淀粉样蛋白β的免疫应答的方法，使用所述淀粉样蛋白β肽预防和治疗阿尔茨海默病的药物，所述淀粉样肽增强的免疫应答，以及包含编码淀粉样蛋白β肽的基因或衍生自淀粉样蛋白β肽的序列的DNA疫苗，其具有加入或插入半胱氨酸或半胱氨酸类似物，如预期类似地有效的。

46. 发明申请

US20130041137A1 von Willebrand Factor (vWF) - Cleaving Protease 审中-公开
标题翻译： von Willebrand因子（vWF） - 切割蛋白酶
公开(公告)号：US20130041137A1
公开(公告)日：2013-02-14
申请号：US13271761
申请日：2011-10-12
申请人： Kenji SOEJIMA , Noriko Mimura , Hiroaki Maeda , Chikateru Nozaki , Takayoshi Hamamoto , Tomohiro Nakagaki
发明人： Kenji SOEJIMA , Noriko Mimura , Hiroaki Maeda , Chikateru Nozaki , Takayoshi Hamamoto , Tomohiro Nakagaki
IPC分类号： C07K16/40
CPC分类号： C12N9/6489 , A61K31/711 , A61K38/00 , C12N9/50 , C12N9/6421
摘要： This invention is intended to isolate and identify a vWF-specific cleaving protease. The vWF-specific cleaving protease cleaves a bond between residues Tyr 842 and Met 843 of vWF and comprises a polypeptide chain having Leu-Leu-Val-Ala-Val (SEQ ID NO: 1) as a partial sequence, and more preferably comprises a polypeptide chain having the partial N-terminal amino acid sequence of a mature protein, Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val (SEQ ID NO: 2), and having a molecular weight of 105 to 160 kDa in SDS-PAGE under reducing or non-reducing conditions. Isolation and identification of this vWF-specific cleaving protease have led to the possibility of replacement therapy for patients having diseases resulting from a deficiency of the protease, such as thrombotic thrombocytopenic purpura.
摘要翻译：本发明旨在分离和鉴定vWF特异性切割蛋白酶。 vWF特异性切割蛋白酶切割vWF的残基Tyr 842和Met 843之间的键，并且包含具有Leu-Leu-Val-Ala-Val（SEQ ID NO：1）作为部分序列的多肽链，更优选包含具有成熟蛋白的部分N-末端氨基酸序列的Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val（SEQ ID NO：2），并且在还原或非还原条件下，在SDS-PAGE中具有105至160kDa的分子量。这种vWF特异性切割蛋白酶的分离和鉴定已经导致了患有由蛋白酶缺乏引起的疾病的患者（例如血栓性血小板减少性紫癜）的替代疗法的可能性。

47. 发明申请

US20120095186A1 POLYION COMPLEX COMPRISING HYDROPHOBIZED POLYAMINO ACID AND USE OF THE SAME 有权
标题翻译：包含疏水性聚氨基酸的聚合物复合物及其用途
公开(公告)号：US20120095186A1
公开(公告)日：2012-04-19
申请号：US13258867
申请日：2010-03-26
申请人： Masanori Baba , Tomofumi Uto , Mitsuru Akashi , Takami Akagi , Chikateru Nozaki , Kazuyoshi Kaminaka
发明人： Masanori Baba , Tomofumi Uto , Mitsuru Akashi , Takami Akagi , Chikateru Nozaki , Kazuyoshi Kaminaka
IPC分类号： C07K2/00 , C07K14/005 , B32B5/16 , C07K14/77
CPC分类号： C07K14/00 , A61K47/645 , A61K47/6929 , C07K7/02 , C08G69/10 , C08G69/48 , Y10T428/2982
摘要： A polyion complex (PIC) or a PIC nanoparticle that may be easily prepared, and that is finally disappeared in vivo due to its suitable biodegradability while exhibiting high stability in vivo, an immunotherapy agent comprising the PIC nanoparticle to which various antigen proteins or peptides may be easily conjugated or incorporated and/or which may be easily mixed with the antigen proteins or peptides, as well as a process for preparing thereof are provided. Specifically, a polyion complex (PIC) comprising a hydrophobized poly(acidic amino acid) and a basic polypeptide, a nanoparticle thereof having a particle shape, an immunotherapy agent comprising the PIC nanoparticle, as well as a process for preparing the PIC, comprising steps of introducing a hydrophobic amino acid to a poly(acidic amino acid) to prepare a hydrophobized poly(acidic amino acid), and dissolving the hydrophobized poly(acidic amino acid) prepared to a buffer, and it is mixed with a basic polypeptide dissolved in a buffer.
摘要翻译：可以容易地制备的polyion复合物（PIC）或PIC纳米颗粒，并且由于其在体内具有高度稳定性的适当的生物降解性而最终在体内消失，含有各种抗原蛋白或肽可能的PIC纳米颗粒的免疫治疗剂可以容易地缀合或掺入和/或可以容易地与抗原蛋白质或肽混合，以及其制备方法。具体而言，包含疏水化聚（酸性氨基酸）和碱性多肽的聚离子复合物（PIC），其具有颗粒形状的纳米颗粒，包含PIC纳米颗粒的免疫治疗剂以及制备PIC的方法，包括步骤将疏水性氨基酸引入聚（酸性氨基酸）以制备疏水化聚（酸性氨基酸），并将制备的疏水化聚（酸性氨基酸）溶解在缓冲液中，并将其与溶于一个缓冲区

48. 发明授权

US08067221B2 Von Willebrand factor (vWF)-cleaving protease 有权
标题翻译： Von Willebrand因子（vWF） - 切蛋白酶
公开(公告)号：US08067221B2
公开(公告)日：2011-11-29
申请号：US12103899
申请日：2008-04-16
申请人： Kenji Soejima , Noriko Mimura , Hiroaki Maeda , Chikateru Nozaki , Takayoshi Hamamoto , Tomohiro Nakagaki
发明人： Kenji Soejima , Noriko Mimura , Hiroaki Maeda , Chikateru Nozaki , Takayoshi Hamamoto , Tomohiro Nakagaki
IPC分类号： C12N9/48 , C12N1/20 , C12N15/00 , C12Q1/37 , C12Q1/00 , C12P21/04 , C07H21/04
CPC分类号： C12N9/6489 , A61K31/711 , A61K38/00 , C12N9/50 , C12N9/6421
摘要： This invention is intended to isolate and identify a vWF-specific cleaving protease. The vWF-specific cleaving protease cleaves a bond between residues Tyr 842 and Met 843 of vWF and comprises a polypeptide chain having Leu-Leu-Val-Ala-Val (SEQ ID NO: 1) as a partial sequence, and more preferably comprises a polypeptide chain having the partial N-terminal amino acid sequence of a mature protein, Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val (SEQ ID NO: 2), and having a molecular weight of 105 to 160 kDa in SDS-PAGE under reducing or non-reducing conditions. Isolation and identification of this vWF-specific cleaving protease have led to the possibility of replacement therapy for patients having diseases resulting from a deficiency of the protease, such as thrombotic thrombocytopenic purpura.
摘要翻译：本发明旨在分离和鉴定vWF特异性切割蛋白酶。 vWF特异性切割蛋白酶切割vWF的残基Tyr 842和Met 843之间的键，并且包含具有Leu-Leu-Val-Ala-Val（SEQ ID NO：1）作为部分序列的多肽链，更优选包含具有成熟蛋白的部分N-末端氨基酸序列的Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val（SEQ ID NO：2），并且在还原或非还原条件下，在SDS-PAGE中具有105至160kDa的分子量。这种vWF特异性切割蛋白酶的分离和鉴定已经导致了患有由蛋白酶缺乏引起的疾病的患者（例如血栓性血小板减少性紫癜）的替代疗法的可能性。

49. 发明申请

US20110081376A1 METHOD FOR USING INACTIVATED JAPANESE ENCEPHALITIS VIRUS PARTICLES AS ADJUVANT 有权
标题翻译：使用灭活的日本食品病毒颗粒作为ADJUVANT的方法
公开(公告)号：US20110081376A1
公开(公告)日：2011-04-07
申请号：US12996236
申请日：2009-05-27
申请人： Makoto Moriyama , Kazuyoshi Kaminaka , Junichi Matsuda , Chikateru Nozaki
发明人： Makoto Moriyama , Kazuyoshi Kaminaka , Junichi Matsuda , Chikateru Nozaki
IPC分类号： A61K39/295 , A61K39/12 , A61P31/12 , A61P37/04 , A61P31/04
CPC分类号： A61K39/00 , A61K2039/5252 , A61K2039/55588 , Y02A50/388 , Y02A50/39 , Y02A50/464 , Y02A50/466 , Y02A50/472 , Y02A50/484
摘要： A method for using inactivated Japanese encephalitis virus particles as an adjuvant of a vaccine is provided. A method for using inactivated Japanese encephalitis virus (JEV) particles as an adjuvant of various vaccines or a mixed vaccine, said JEV particles being obtained by inoculating JEV Beijing-1 strain to Vero cells, culturing said JEV-infected cells to give cultured cells or culture supernatant, purifying JEV particles from said cultured cells or culture supernatant and inactivating said JEV particles with formalin, a method for preparing a (mixed) vaccine which comprises a step of letting inactivated Japanese encephalitis virus be contained, and a mixed vaccine prepared by said method.
摘要翻译：提供了使用灭活的日本脑炎病毒颗粒作为疫苗佐剂的方法。使用灭活的日本脑炎病毒（JEV）颗粒作为各种疫苗或混合疫苗的佐剂的方法，所述JEV颗粒通过将JEV Beijing-1菌株接种到Vero细胞获得，培养所述JEV感染的细胞以产生培养的细胞或培养上清液，从所述培养细胞或培养上清液中纯化JEV颗粒，并用福尔马林灭活所述JEV颗粒，制备包含使灭活的日本脑炎病毒的步骤的（混合）疫苗的方法，以及由所述方法。

50. 发明申请

US20080254527A1 VON WILLEBRAND FACTOR (vWF) - CLEAVING PROTEASE 有权
标题翻译： VON WILLEBRAND因子（vWF） - 清洁保护
公开(公告)号：US20080254527A1
公开(公告)日：2008-10-16
申请号：US12103899
申请日：2008-04-16
申请人： Kenji SOEJIMA , Noriko Mimura , Hiroaki Maeda , Chikateru Nozaki , Takayoshi Hamamoto , Tomohiro Nakagaki
发明人： Kenji SOEJIMA , Noriko Mimura , Hiroaki Maeda , Chikateru Nozaki , Takayoshi Hamamoto , Tomohiro Nakagaki
IPC分类号： C12N9/48 , C07K16/00
CPC分类号： C12N9/6489 , A61K31/711 , A61K38/00 , C12N9/50 , C12N9/6421
摘要： This invention is intended to isolate and identify a vWF-specific cleaving protease. The vWF-specific cleaving protease cleaves a bond between residues Tyr 842 and Met 843 of vWF and comprises a polypeptide chain having Leu-Leu-Val-Ala-Val (SEQ ID NO: 1) as a partial sequence, and more preferably comprises a polypeptide chain having the partial N-terminal amino acid sequence of a mature protein, Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val (SEQ ID NO: 2), and having a molecular weight of 105 to 160 kDa in SDS-PAGE under reducing or non-reducing conditions. Isolation and identification of this vWF-specific cleaving protease have led to the possibility of replacement therapy for patients having diseases resulting from a deficiency of the protease, such as thrombotic thrombocytopenic purpura.
摘要翻译：本发明旨在分离和鉴定vWF特异性切割蛋白酶。 vWF特异性切割蛋白酶切割vWF的残基Tyr 842和Met 843之间的键，并且包含具有Leu-Leu-Val-Ala-Val（SEQ ID NO：1）作为部分序列的多肽链，更优选包含具有成熟蛋白的部分N-末端氨基酸序列的Ala-Ala-Gly-Gly-Ile-Leu-His-Leu-Glu-Leu-Leu-Val-Ala-Val（SEQ ID NO：2），并且在还原或非还原条件下，在SDS-PAGE中具有105至160kDa的分子量。这种vWF特异性切割蛋白酶的分离和鉴定已经导致了患有由蛋白酶缺乏引起的疾病的患者（例如血栓性血小板减少性紫癜）的替代疗法的可能性。

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