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41. 发明授权

US07927825B2 Methods of using T1R2/T1R3 heteromeric receptors to quantify taste of compounds for food or beverages 有权
标题翻译：使用T1R2 / T1R3异聚受体量化食物或饮料化合物味道的方法
公开(公告)号：US07927825B2
公开(公告)日：2011-04-19
申请号：US12270830
申请日：2008-11-13
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： C01N33/567 , C07K1/00 , C07K14/00 , C07K17/00
CPC分类号： G01N33/5041 , A01K2217/05 , C07K14/705 , C07K2319/00 , C12Q1/6876 , C12Q2600/124 , C12Q2600/158 , G01N33/5008 , G01N33/566 , G01N2333/4719 , G01N2333/705 , G01N2333/726 , G01N2500/04
摘要： Methods of quantifying the taste of compounds for food and beverages are provided. These methods comprise contacting the compounds with an isolated heteromeric receptor comprising at least one T1R2 polypeptide and at least one T1R3 polypeptide.
摘要翻译：提供了定量食品和饮料化合物味道的方法。这些方法包括使化合物与包含至少一种T1R2多肽和至少一种T1R3多肽的分离的异聚受体接触。

42. 发明申请

US20090221798A1 HETEROMERIC TASTE RECEPTORS 有权
标题翻译：异体吸收受体
公开(公告)号：US20090221798A1
公开(公告)日：2009-09-03
申请号：US12270857
申请日：2008-11-14
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： C07K14/00
CPC分类号： G01N33/5008 , A01K2217/05 , C07K14/705 , G01N33/5041 , G01N33/566 , G01N2333/726
摘要： Heteromeric taste receptors are provided. These receptors comprise a first polypeptide which comprises extracellular and transmembrane domains wherein the transmembrane domains are at least 95% identical to the transmembrane domains of specific T1R2 polypeptides, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of the specific T1R2 polypeptide or a different GPCR; and a second polypeptide which comprises extracellular and transmembrane domains wherein the transmembrane domains are at least 95% identical to the transmembrane domains of specific T1R3 polypeptides, and the extracellular domains are at least 95% identical to the corresponding extracellular domains of the specific T1R3 polypeptide or that of a different GPCR.
摘要翻译：提供异味味觉受体。这些受体包含第一多肽，其包含细胞外和跨膜结构域，其中跨膜结构域与特异性T1R2多肽的跨膜结构域至少95％相同，并且细胞外结构域与特异性T1R2的相应胞外结构域至少95％相同多肽或不同的GPCR; 和包含细胞外和跨膜结构域的第二多肽，其中所述跨膜结构域与特异性T1R3多肽的跨膜结构域至少95％相同，并且细胞外结构域与特异性T1R3多肽的相应细胞外结构域至少95％相同，或不同的GPCR。

43. 发明申请

US20090221001A1 Methods for identifying compounds that modulate the T1R1/T1R3 umami taste receptor 有权
标题翻译：鉴定调节T1R1 / T1R3鲜味受体的化合物的方法
公开(公告)号：US20090221001A1
公开(公告)日：2009-09-03
申请号：US12270838
申请日：2008-11-13
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： G01N33/566
CPC分类号： G01N33/5008 , C12N2503/00 , C12N2510/00 , C40B30/04 , G01N33/6872 , G01N2333/726 , G01N2500/10
摘要： Methods for identifying compounds that modulate the T1R1/T1R3 umami taste receptors are provided. These methods comprise screening for compounds that compete with lactisole for binding to and/or inhibiting the T1R1/T1R3 umami taste receptor.
摘要翻译：提供了鉴定调节T1R1 / T1R3鲜味受体的化合物的方法。这些方法包括筛选与乳清蛋白竞争结合和/或抑制T1R1 / T1R3鲜味受体的化合物。

44. 发明申请

US20080262087A1 T1R HETERO-OLIGOMERIC TASTE RECEPTORS AND CELL LINES THAT EXPRESS SAID RECEPTORS AND USE THEREOF FOR IDENTIFICATION OF TASTE COMPOUNDS 审中-公开
公开(公告)号：US20080262087A1
公开(公告)日：2008-10-23
申请号：US11932949
申请日：2007-10-31
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： A61K31/192 , C07K14/705 , C12N5/08 , C12N5/06 , C07K17/00 , A61P27/00 , G01N33/566 , G01N33/53 , C40B30/04 , C12Q1/68
CPC分类号： G01N33/5041 , A01K2217/05 , C07K14/705 , C07K2319/00 , C12Q1/6876 , C12Q2600/124 , C12Q2600/158 , G01N33/5008 , G01N33/566 , G01N2333/4719 , G01N2333/705 , G01N2333/726 , G01N2500/04
摘要： The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners.Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli.Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.Finally, the invention relates to the discovery that some compounds, e.g., lactisole, inhibit both the activities of human T1R2/T1R3 and T1R1/T1R3 receptors, and accordingly the sweet and umami taste, suggesting that these receptors may be the only sweet and umami receptors.

45. 发明申请

US20080244762A1 T1R HETERO-OLIGOMERIC TASTE RECEPTORS AND CELL LINES THAT EXPRESS SAID RECEPTORS AND USE THEREOF FOR IDENTIFICATION OF TASTE COMPOUNDS 审中-公开
公开(公告)号：US20080244762A1
公开(公告)日：2008-10-02
申请号：US11939408
申请日：2007-11-13
申请人： Mark ZOLLER , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fermando Echeveri
发明人： Mark ZOLLER , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fermando Echeveri
IPC分类号： A01K67/027 , C07K14/00 , G01N33/566 , G01N33/53 , C40B30/04 , C12N5/06 , G01N33/569
CPC分类号： G01N33/5008 , A01K2217/05 , C07K14/705 , G01N33/5041 , G01N33/566 , G01N2333/726
摘要： The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners.Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli.Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.Finally, the invention relates to the discovery that some compounds, e.g., lactisole, inhibit both the activities of human T1R2/T1R3 and T1R1/T1R3 receptors, and accordingly the sweet and umami taste, suggesting that these receptors may be the only sweet and umami receptors.

46. 发明申请

US20080244761A1 T1R HETERO-OLIGOMERIC TASTE RECEPTORS AND CELL LINES THAT EXPRESS SAID RECEPTORS AND USE THEREOF FOR IDENTIFICATION OF TASTE COMPOUNDS 审中-公开
公开(公告)号：US20080244761A1
公开(公告)日：2008-10-02
申请号：US11932989
申请日：2007-10-31
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： A01K67/027 , C07K14/00 , G01N33/566 , C40B30/04 , G01N33/569
CPC分类号： G01N33/5008 , C12N2503/00 , C12N2510/00 , C40B30/04 , G01N33/6872 , G01N2333/726 , G01N2500/10
摘要： The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners.Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli.Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.Finally, the invention relates to the discovery that some compounds, e.g., lactisole, inhibit both the activities of human T1R2/T1R3 and T1R1/T1R3 receptors, and accordingly the sweet and umami taste, suggesting that these receptors may be the only sweet and umami receptors.

47. 发明授权

US07297543B2 Cell lines that stably or transiently express a functional sweet (T1R2/T1R3) taste receptor 有权
标题翻译：稳定或瞬时表达功能性甜（T1R2 / T1R3）味觉受体的细胞系
公开(公告)号：US07297543B2
公开(公告)日：2007-11-20
申请号：US10725103
申请日：2003-12-02
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： C12N15/74 , C12N5/00 , C12N5/02 , C07K1/00 , C07K14/00
CPC分类号： G01N33/5041 , A01K2217/05 , C07K14/705 , C07K2319/00 , C12Q1/6876 , C12Q2600/124 , C12Q2600/158 , G01N33/5008 , G01N33/566 , G01N2333/4719 , G01N2333/705 , G01N2333/726 , G01N2500/04
摘要： Cells are engineered to stably or transiently express T1R2 and T1R3 polypeptides, desirably in association with a G protein. The resultant heteromeric taste receptor is a sweet taste receptor and responds to sweet taste stimuli such as artificial and natural sweeteners, e.g., saccharin. The invention provides a preferred mammalian cell lines, e.g., HEK-293 cells that stably express T1R2/T1R3 and Gα15 under inducible conditions. These cells are useful in cell-based assays for identifying compounds that elicit or modulate sweet taste.
摘要翻译：工程改造细胞以稳定或瞬时表达T1R2和T1R3多肽，理想地与G蛋白结合。所得的异味味觉受体是甜味受体并对甜味刺激如人造天然甜味剂例如糖精作出反应。本发明提供优选的哺乳动物细胞系，例如在可诱导条件下稳定表达T1R2 / T1R3和Gα15α的HEK-293细胞。这些细胞可用于鉴定引发或调节甜味的化合物的基于细胞的测定。

48. 发明申请

US20130004961A1 METHODS OF DETECTING A SWEET-SENSITIVE CELL 有权
标题翻译：检测敏感细胞的方法
公开(公告)号：US20130004961A1
公开(公告)日：2013-01-03
申请号：US13477907
申请日：2012-05-22
申请人： Mark Zoller , Xiaodong Li , Lana Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lana Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Elliot Adler , Hong Xu , Fernando Echeverri
IPC分类号： G01N33/566 , C12N15/85 , C07K14/705 , C12Q1/68 , C12P21/02 , C12N15/12 , C12N5/10
CPC分类号： G01N33/5041 , A01K2217/05 , C07K14/705 , C07K2319/00 , C12Q1/6876 , C12Q2600/124 , C12Q2600/158 , G01N33/5008 , G01N33/566 , G01N2333/4719 , G01N2333/705 , G01N2333/726 , G01N2500/04
摘要： The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners.Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli.
摘要翻译：本发明涉及T1R受体组装形成功能性味觉受体的发现。特别地，已经发现，T1R1和T1R3的共表达导致对鲜味刺激（包括谷氨酸钠）起反应的味觉感受器。此外，已经发现，T1R2和T1R3受体的共表达导致对包括天然存在的和人造甜味剂的甜味刺激有反应的味觉受体。本发明还涉及在测定中使用包含T1R1 / T1R3和T1R2 / T1R3的异低聚味觉感受器来鉴定分别响应于鲜味刺激和甜味刺激的化合物。

49. 发明申请

US20080020424A9 HETEROMERIC UMAMI T1R1/T1R3 TASTE RECEPTORS AND ISOLATED CELLS THAT EXPRESS SAME 有权
标题翻译：异位UMAMI T1R1 / T1R3具有明显特异性的受体和分离的细胞
公开(公告)号：US20080020424A9
公开(公告)日：2008-01-24
申请号：US10179373
申请日：2002-06-26
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Adler , Hong Xu , Fernando Echeverri
IPC分类号： C07K14/705 , C12P21/02 , C12N5/06 , C07H21/04
CPC分类号： G01N33/5041 , A01K2217/05 , C07K14/705 , C07K2319/00 , C12Q1/6876 , C12Q2600/124 , C12Q2600/158 , G01N33/5008 , G01N33/566 , G01N2333/4719 , G01N2333/705 , G01N2333/726 , G01N2500/04
摘要： The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners. Also the present invention relates to the use of hetero-oligomeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging. Finally, the invention relates to the discovery that some compounds, e.g., lactisole, inhibit both the activities of human T1R2/T1R3 and T1R1/T1R3 receptors, and accordingly the sweet and umami taste, suggesting that these receptors may be the only sweet and umami receptors.
摘要翻译：本发明涉及T1R受体组装形成功能性味觉受体的发现。特别地，已经发现，T1R1和T1R3的共表达导致对鲜味刺激（包括谷氨酸钠）起反应的味觉感受器。此外，已经发现，T1R2和T1R3受体的共表达导致对包括天然存在的和人造甜味剂的甜味刺激有反应的味觉受体。本发明还涉及在测定中使用包含T1R1 / T1R3和T1R2 / T1R3的异低聚味觉感受器来鉴定分别响应于鲜味刺激和甜味刺激的化合物。此外，本发明涉及稳定或瞬时共表达T1R1和T1R3的组合的细胞系的组成; 或T1R2和T1R3; 在组成型或诱导性条件下。还提供了在基于细胞的测定中使用这些细胞系以鉴定鲜味和甜味调节化合物，特别是通过使用荧光成像检测受体活性的高通量筛选测定。最后，本发明涉及一些发现，一些化合物，例如乳酸，抑制人T1R2 / T1R3和T1R1 / T1R3受体的活性，因此抑制甜味和鲜味，这表明这些受体可能是唯一的甜味和鲜味受体。

50. 发明申请

US20060127977A1 Isolated (T1R2/T1R3) sweet taste receptors that respond to sweet taste stimuli 有权
标题翻译：分离（T1R2 / T1R3）甜味受体，对甜味刺激有反应
公开(公告)号：US20060127977A1
公开(公告)日：2006-06-15
申请号：US10725037
申请日：2003-12-02
申请人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Adler , Hong Xu , Fernando Echeverri
发明人： Mark Zoller , Xiaodong Li , Lena Staszewski , Shawn O'Connell , Sergey Zozulya , Jon Adler , Hong Xu , Fernando Echeverri
IPC分类号： A61K38/17 , C07K14/705 , C12N5/06 , C07H21/04 , C12P21/06
CPC分类号： G01N33/5041 , A01K2217/05 , C07K14/705 , C07K2319/00 , C12Q1/6876 , C12Q2600/124 , C12Q2600/158 , G01N33/5008 , G01N33/566 , G01N2333/4719 , G01N2333/705 , G01N2333/726 , G01N2500/04
摘要： Isolated or biologically pure taste receptors comprised of at least one T1R2 and T1R3 polypeptide and compositions containing are provided. These taste receptors specifically bind and/or are specifically activated by sweet taste stimuli such as natural and artificial sweetener compounds. These taste receptors are useful in screening assays for identifying compounds that elicit or modulate (enhance or inhibit) sweet taste. These compounds can be used as additives and flavorants to improve the taste of compositions for human or animal consumption. In preferred embodiments, the T1R2/T1R3 receptor will be comprised of human T1R2/human T1R3 expressed in association with a suitable G protein, preferably Gα15.
摘要翻译：提供了由至少一种T1R2和T1R3多肽组成的分离的或生物学上纯的味觉受体和含有的组合物。这些味觉受体特异性结合和/或由甜味刺激物例如天然和人造甜味剂化合物特异性活化。这些味觉受体可用于鉴定诱导或调节（增强或抑制）甜味的化合物的筛选测定。这些化合物可用作添加剂和调味剂，以改善用于人或动物消费的组合物的味道。在优选的实施方案中，T1R2 / T1R3受体将由与合适的G蛋白（优选Gα15）相关联表达的人T1R2 /人T1R3组成。

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