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    • 42. 发明申请
    • VECTOR FOR IDENTIFICATION, SELECTION AND EXPRESSION OF RECOMBINANTS
    • 鉴定,选择和表达重组体的载体
    • US20110165583A1
    • 2011-07-07
    • US13061640
    • 2009-09-02
    • Anjali Apte DeshpandeSampali BanerjeeJitendra KumarSriram Padmanabhan
    • Anjali Apte DeshpandeSampali BanerjeeJitendra KumarSriram Padmanabhan
    • C12Q1/68C12N15/74C12N15/63
    • C12N15/64C12N15/10C12N15/65
    • A modified vector comprising a reporter gene having a STOP codon upstream of the multiple cloning site of the vector which is characterized in that the recombinant clones show fluoresce or show color in presence of inducer. A method for identification and selection of recombinant clones comprising the modified vector wherein the recombinant clones florescence or show color in a suitable suppressor strain of the STOP codon associated with the gene of interest. A method of preparation of recombinant clone comprising gene of interest and modified vector comprising amplification of gene of interest using specific primers containing STOP codon different from STOP codon used with reporter gene; cloning the amplified gene of interest in the modified vector; transformation of cloned modified vector in the STOP codon suppressor host cell wherein the STOP codon suppressor host cell is specific for STOP codon used with the gene of interest wherein the recombinant clones either fluorescence or show color depending upon the reporter gene used.
    • 包含在载体的多克隆位点上游具有一个末端密码子的报道基因的修饰载体,其特征在于重组克隆在诱导物存在下显示出荧光或显示出颜色。 用于鉴定和选择包含修饰载体的重组克隆的方法,其中所述重组克隆在与所关心的基因相关的STOP密码子的合适的抑制菌株中荧光或显示颜色。 包含目的基因和修饰载体的重组克隆的制备方法,其包括使用与报告基因使用的不同于STOP密码子的不同密码子的特异性引物扩增目标基因; 在经修饰的载体中克隆所扩增的目的基因; 克隆的修饰载体在STOP密码子抑制基因宿主细胞中的转化,其中所述的停止密码子抑制基因宿主细胞对于与感兴趣的基因一起使用的终止密码子是特异性的,其中所述重组克隆根据所使用的报道基因是荧光或显示颜色。
    • 44. 发明申请
    • Speed selective table scan operation
    • 速度选择表扫描操作
    • US20080091908A1
    • 2008-04-17
    • US11548889
    • 2006-10-12
    • Bishwaranjan BhattacharjeeChristian A. LangTimothy R. MalkemusSriram PadmanabhanKwai Wong
    • Bishwaranjan BhattacharjeeChristian A. LangTimothy R. MalkemusSriram PadmanabhanKwai Wong
    • G06F12/00
    • G06F12/0866G06F12/123G06F17/30448
    • Disclosed are a method, information processing system, and computer readable medium for scanning a storage medium table. The method includes retrieving location information associated with at least one other storage medium table scan. A storage medium table scan is started at a location within a storage medium table based on at least a location of the one other storage medium table scan. A weight is assigned to at least one storage medium block based on at least a current scanning location within the storage medium table relative to the location of the one other table scan. The method determines if a distance between the current scanning location and the location of the one other table scan is greater than a first given threshold. A current scanning operation is delayed, in response to the distance being greater than the given threshold, until the distance is below a second given threshold.
    • 公开了一种用于扫描存储介质表的方法,信息处理系统和计算机可读介质。 该方法包括检索与至少一个其他存储介质表扫描相关联的位置信息。 基于至少另一个存储介质表扫描的位置,在存储介质表中的位置处开始存储介质表扫描。 基于至少一个存储介质表中的当前扫描位置相对于另一个表扫描的位置,将权重分配给至少一个存储介质块。 该方法确定当前扫描位置与另一个表扫描的位置之间的距离是否大于第一给定阈值。 响应于距离大于给定阈值,当前扫描操作被延迟,直到距离低于第二给定阈值。