专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

31. 发明授权

US06632671B2 Nanoparticle encapsulation system and method 有权
标题翻译：纳米粒子封装体系及方法
公开(公告)号：US06632671B2
公开(公告)日：2003-10-14
申请号：US09796575
申请日：2001-02-28
申请人： Gretchen M. Unger
发明人： Gretchen M. Unger
IPC分类号： C12N1588
CPC分类号： B01J13/08 , A61K9/1075 , A61K9/5138 , A61K9/5161 , A61K9/5192 , B01J13/02 , Y10S977/773 , Y10S977/795 , Y10S977/897 , Y10S977/915 , Y10S977/92
摘要： The present invention generally relates to nanocapsules and methods of preparing these nanocapsules. The present invention includes a method of forming a surfactant micelle and dispersing the surfactant micelle into an aqueous composition having a hydrophilic polymer to form a stabilized dispersion of surfactant micelles. The method further includes mechanically forming droplets of the stabilized dispersion of surfactant micelles, precipitating the hydrophilic polymer to form precipitated nanocapsules, incubating the nanocapsules to reduce a diameter of the nanocapsules, and filtering or centrifuging the nanocapsules.
摘要翻译：本发明一般涉及纳米胶囊和制备这些纳米胶囊的方法。本发明包括形成表面活性剂胶束并将表面活性剂胶束分散到具有亲水性聚合物的水性组合物中以形成稳定的表面活性剂胶束分散体的方法。该方法还包括机械地形成稳定的表面活性剂胶束分散体的液滴，沉淀亲水性聚合物以形成沉淀的纳米胶囊，孵育纳米胶囊以减小纳米胶囊的直径，以及过滤或离心纳米胶囊。

32. 发明授权

US06573039B1 System for cell-based screening 有权
公开(公告)号：US06573039B1
公开(公告)日：2003-06-03
申请号：US09650937
申请日：2000-08-29
申请人： R. Terry Dunlay , D. Lansing Taylor
发明人： R. Terry Dunlay , D. Lansing Taylor
IPC分类号： G01N3353
CPC分类号： B82Y30/00 , B01J2219/00315 , B01J2219/00317 , B01J2219/00707 , B01J2219/00743 , B01L3/5085 , C12Q1/68 , C12Q1/6813 , C40B60/14 , G01N15/1475 , G01N21/6428 , G01N21/6452 , G01N21/6458 , G01N33/5005 , G01N33/5035 , G01N33/582 , G01N2015/1486 , G01N2015/1497 , G01N2021/6419 , G01N2021/6482 , G02B21/16 , G02B21/365 , Y10S436/80 , Y10S436/807 , Y10S436/809 , Y10S977/795 , Y10S977/881 , Y10S977/92 , Y10S977/941 , Y10S977/942 , C12Q2525/179
摘要： The invention relates to an optical system for determining the distribution, environment, or activity of fluorescently labeled reporter molecules in cells for the purpose of screening large numbers of compounds for specific biological activity. The invention involves providing cells containing fluorescent reporter molecules in an array of locations and scanning numerous cells in each location with a fluorescent microscope, converting the optical information into digital data, and utilizing the digital data to determine the distribution, environment or activity of the fluorescently labeled reporter molecules in the cells. The array of locations may be an industry standard 96 well or 384 well microtiter plate or a microplate which is a microplate having a cells in a micropaterned array of locations. The invention includes apparatus and computerized method for processing, displaying and storing the data.

33. 发明授权

US06558676B1 Parvovirus capsids 有权
标题翻译：细小病毒衣壳
公开(公告)号：US06558676B1
公开(公告)日：2003-05-06
申请号：US09611066
申请日：2000-07-06
申请人： Neal S. Young , Sachiko Kajigaya , Shimada Takashi
发明人： Neal S. Young , Sachiko Kajigaya , Shimada Takashi
IPC分类号： A61K3923
CPC分类号： C07K14/005 , A61K39/00 , C07K16/081 , C12N15/86 , C12N2710/14043 , C12N2750/14122 , C12N2750/14222 , C12N2750/14223 , C12N2750/14243 , C12N2750/14322 , C12N2750/14323 , C12N2750/14343 , Y10S977/742 , Y10S977/802 , Y10S977/803 , Y10S977/898 , Y10S977/917 , Y10S977/92 , Y10S977/927
摘要： The present invention relates to a method of producing non-infections parvovirus capsids and to diagnostic assays and vaccines utilizing same. The invention further relates to recombinant baculoviruses encoding parvovirus structural proteins and host cells infected therewith. The invention also relates to a method of packaging and delivering genetic information utilizing the noninfectious capsids.
摘要翻译：本发明涉及产生非感染细小病毒衣壳的方法以及利用其的诊断测定和疫苗。本发明还涉及编码细小病毒结构蛋白的重组杆状病毒和其感染的宿主细胞。本发明还涉及利用非传染性衣壳包装和递送遗传信息的方法。

34. 发明授权

US06470220B1 Diagnosis and treatment of cancers using in vivo magnetic domains 失效
标题翻译：使用体内磁畴诊断和治疗癌症
公开(公告)号：US06470220B1
公开(公告)日：2002-10-22
申请号：US09538113
申请日：2000-03-29
申请人： Robert H. Kraus, Jr. , Don M. Coates
发明人： Robert H. Kraus, Jr. , Don M. Coates
IPC分类号： A61F200
CPC分类号： A61N2/00 , A61K41/0052 , Y10S977/775 , Y10S977/808 , Y10S977/904 , Y10S977/92 , Y10S977/959 , Y10S977/96
摘要： A method of detecting and treating tumors within a subject is disclosed together with a process for the non-invasive measurement of tissue temperature within a subject.
摘要翻译：公开了一种检测和治疗受试者内的肿瘤的方法，以及用于非侵入式测量受试者内组织温度的方法。

35. 发明授权

US06368800B1 Kits for isolating biological target materials using silica magnetic particles 有权
标题翻译：使用二氧化硅磁性颗粒分离生物靶材的试剂盒
公开(公告)号：US06368800B1
公开(公告)日：2002-04-09
申请号：US09459502
申请日：1999-12-13
申请人： Craig E. Smith , Charles K. York
发明人： Craig E. Smith , Charles K. York
IPC分类号： C12Q168
CPC分类号： C12N15/1013 , C12Q1/6806 , Y10S977/838 , Y10S977/904 , Y10S977/916 , Y10S977/92 , Y10S977/924 , Y10S977/96
摘要： The present invention provides methods for isolating biological target materials, particularly nucleic acids, such as DNA or RNA or hybrid molecules of DNA and RNA, from other substances in a medium using silica magnetic particles. The methods of the present invention involve forming a complex of the silica magnetic particles and the biological target material in a mixture of the medium and particles, separating the complex from the mixture using external magnetic force, and eluting the biological target material from the complex. The preferred embodiments of magnetic silica particles used in the methods and kits of the present invention are capable of forming a complex with at least 2 &mgr;g of biological target material per milligram of particle, and of releasing at least 60% of the material from the complex in the elution step of the method. The methods of the present invention produce isolated biological target material which is substantially free of contaminants, such as metals or macromolecular substances, which can interfere with further processing or analysis, if present.
摘要翻译：本发明提供了使用二氧化硅磁性颗粒从介质中的其他物质分离生物靶材料，特别是DNA或RNA或DNA和RNA的混合分子的方法。本发明的方法包括在介质和颗粒的混合物中形成二氧化硅磁性颗粒和生物目标材料的复合物，使用外部磁力从混合物中分离复合物，并从复合物中洗脱生物目标物质。在本发明的方法和试剂盒中使用的磁性二氧化硅颗粒的优选实施方案能够与每毫克颗粒至少2个生物靶材料形成复合物，并且从复合物中释放至少60％的材料在该方法的洗脱步骤中。本发明的方法产生基本上不含污染物（例如金属或大分子物质）的分离的生物靶材料，如果存在可能会干扰进一步的加工或分析。

36. 发明授权

US06342372B1 Eukaryotic layered vector initiation systems for production of recombinant proteins 有权
标题翻译：用于重组蛋白质生产的真核分层载体启动系统
公开(公告)号：US06342372B1
公开(公告)日：2002-01-29
申请号：US09350399
申请日：1999-07-08
申请人： Thomas W. Dubensky, Jr. , John M. Polo , David A. Driver
发明人： Thomas W. Dubensky, Jr. , John M. Polo , David A. Driver
IPC分类号： C12N516
CPC分类号： C12N15/113 , A61K38/00 , A61K48/00 , A61K2039/5256 , C07K14/005 , C07K14/47 , C07K14/705 , C07K14/755 , C07K14/82 , C12N9/2402 , C12N15/1131 , C12N15/86 , C12N2310/111 , C12N2310/122 , C12N2710/10322 , C12N2710/16722 , C12N2730/10122 , C12N2740/15022 , C12N2740/16222 , C12N2770/36122 , C12N2830/002 , C12N2830/42 , C12N2830/60 , C12N2840/20 , C12N2840/203 , C12N2840/206 , C12N2840/44 , C12N2840/85 , C12Y302/01045 , Y02A50/388 , Y02A50/396 , Y02A50/466 , Y10S977/773 , Y10S977/804 , Y10S977/808 , Y10S977/918 , Y10S977/92 , Y10S977/924
摘要： The present invention provides compositions and methods for utilizing recombinant alphavirus vectors. Also disclosed are compositions and methods for making and utilizing eukaryotic layered vector initiation systems.
摘要翻译：本发明提供了利用重组甲病毒载体的组合物和方法。还公开了用于制备和利用真核分层载体引发系统的组合物和方法。

37. 发明授权

US06308509B1 Fibrous structures containing nanofibrils and other textile fibers 有权
标题翻译：含纤维结构的纳米纤维和其他纺织纤维
公开(公告)号：US06308509B1
公开(公告)日：2001-10-30
申请号：US09621856
申请日：2000-07-24
申请人： Frank L. Scardino , Richard J. Balonis
发明人： Frank L. Scardino , Richard J. Balonis
IPC分类号： D01H428
CPC分类号： D01D5/0084 , A61F2/06 , A61F2/08 , A61F2/28 , A61F2/30756 , A61F2/3094 , A61F2/30965 , A61F2002/2835 , A61F2002/30062 , A61F2002/30199 , A61F2002/3084 , A61F2002/30914 , A61F2002/30968 , A61F2002/4648 , A61F2210/0004 , A61F2230/0063 , A61F2310/00293 , A61L27/12 , D01D5/0076 , D01H4/02 , D02G3/36 , D02G3/448 , Y10S977/855 , Y10S977/876 , Y10S977/92 , Y10S977/961 , Y10T428/1369 , Y10T428/2936
摘要： Nanofibers are produced having a diameter ranging from about 4 Å to 1 nm, and a nano denier of about 10−9. The use of the electro-spinning process permits production of the desired nanofibrils. These fibrils in combination with a carrier or strengthening fibers/filaments can be converted directly into nonwoven fibrous assemblies or converted into linear assemblies(yarns) before weaving, braiding or knitting into 2-dimensional and 3-dimensional fabrics. The electrospun fiber can be fed in an air vortex spinning apparatus developed to form a linear fibrous assembly. The process makes use of an air stream in a properly confined cavity. The vortex of air provides a gentle means to convert a mixture of the fibril fed directly or indirectly from the ESP unit and a fiber mass or filament into an integral assembly with proper level of orientation. Incorporation of thus produced woven products into tissue engineering is part of the present invention.
摘要翻译：生产直径为约4至1nm的纳米纤维和约10-9纳米旦尼尔的纳米纤维。使用电纺工艺可以生产所需的纳米原纤维。这些原纤维与载体或强化纤维/长丝组合可以直接转化成非织造纤维组件，或者在编织，编织或编织成二维和三维织物之前转化成线性组件（纱线）。电纺纤维可以在开发成形成线性纤维集合体的空气涡旋纺丝装置中进料。该过程使用适当限定的空腔中的气流。空气涡流提供了将来自ESP单元和纤维质量或细丝直接或间接进料的原纤维的混合物转化为具有适当水平取向的整体组件的温和装置。将如此生产的织造产品并入组织工程中是本发明的一部分

38. 发明申请

US20010016355A1 AAV capsid vehicles for molecular transfer 审中-公开
公开(公告)号：US20010016355A1
公开(公告)日：2001-08-23
申请号：US09746246
申请日：2000-12-22
申请人： University of Pittsburgh
发明人： Richard Jude Samulski , Forrest K. Ferrari
IPC分类号： C12N007/01 , C12N015/861
CPC分类号： C12N15/86 , A61K39/00 , A61K48/00 , C07K14/005 , C12N7/00 , C12N2710/10344 , C12N2750/14122 , C12N2750/14123 , C12N2750/14143 , Y10S977/804 , Y10S977/84 , Y10S977/918 , Y10S977/92
摘要： The invention relates to the production of AAV capsids which may be used to transfer native or heterologous molecules into appropriate host cells. The capsid proteins can be expressed from a recombinant virus, expression vector, or from a cell line that has stably integrated the AAV capsid genes or coding sequences. The invention further provides for the production of AAV capsids in vitro from the AAV capsid proteins and the construction of packaged capsids in vitro. The invention further provides for the production of AAV capsids that have been genetically engineered to express heterologous epitopes of clinically important antigens to elicit an immune response.

39. 发明授权

US06207195B1 Therapeutic nanospheres 失效
标题翻译：治疗纳米球
公开(公告)号：US06207195B1
公开(公告)日：2001-03-27
申请号：US09095882
申请日：1998-06-11
申请人： Scott Walsh , Ronald Rubenstein , Pam Zeitlin , Kam W. Leong
发明人： Scott Walsh , Ronald Rubenstein , Pam Zeitlin , Kam W. Leong
IPC分类号： C12N1500
CPC分类号： A61K9/5169 , A61K31/192 , A61K45/06 , A61K47/62 , A61K47/6435 , A61K47/6929 , A61K48/00 , B82Y5/00 , Y10S977/884 , Y10S977/906 , Y10S977/915 , Y10S977/92 , Y10S977/923
摘要： 4-Phenylbutyrate exerts many beneficial biological effects. It appears to induce the transcription of certain promoters, as well as having a remedial effect on proteins which are aberrantly localized within the cell. In addition, it appears to cause cells to developmentally differentiate. The present invention provides nanosphere formulations of 4-phenylbutyrate and other drugs which remediate defective protein localization intracellularly. These formulations permit lower concentrations of drugs to be administered, providing both cost and safety benefits.
摘要翻译： 4-苯基丁酸酯具有许多有益的生物学效应。似乎诱导某些启动子的转录，以及对细胞内异常定位的蛋白质具有补救作用。此外，它似乎导致细胞发育分化。本发明提供4-苯基丁酸酯和其它药物的纳米球配方，其可以修复细胞内的缺陷蛋白质定位。这些制剂允许施用较低浓度的药物，既提供成本和安全性益处。

40. 发明授权

US06177265B1 Process for the manufacture of a starch hydrolysate with high content 失效
标题翻译：制备高含量淀粉水解产物的方法
公开(公告)号：US06177265B1
公开(公告)日：2001-01-23
申请号：US09328951
申请日：1999-06-09
申请人： Pierrick Duflot
发明人： Pierrick Duflot
IPC分类号： C12P1900
CPC分类号： C12P7/18 , C12P19/20 , Y10S977/898 , Y10S977/902 , Y10S977/92
摘要： The invention concerns a process for the manufacture of a starch hydrolysate with high dextrose content comprising the stages of: (a) liquefying a starch milk with the aid of an &agr;-amylase so as to obtain a liquefied starch milk; (b) saccharifying the liquefied starch milk, with the aid of a glucogenic enzyme, to obtain a raw saccharified hydrolysate having a maximum content of 80% by weight, and preferably 75% by weight; (c) microfiltering the raw saccharified hydrolysate so as to collect a microfiltration permeate and a microfiltration retentate; (d) separating the microfiltration permeate by nanofiltration over membranes so as to collect a nanofiltration permeate constituting said starch hydrolysate with high dextrose content and a nanofiltration retentate.
摘要翻译：本发明涉及制备具有高葡萄糖含量的淀粉水解产物的方法，其包括以下阶段：（a）借助α-淀粉酶液化淀粉，以获得液化的淀粉乳;（b）将液化的淀粉乳，借助于葡萄糖原酶，得到最大含量为80重量％，优选为75重量％的生糖化水解产物;（c）对生糖化水解产物进行微过滤以收集微滤渗透物和微滤滞留物;（d）通过膜上的纳滤过滤分离微滤渗透物，以收集构成具有高葡萄糖含量的淀粉水解产物和纳滤膜渗余物的纳滤渗透物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式