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31. 发明授权

US09068208B2 Mutant endoglycoceramidases with enhanced synthetic activity 有权
标题翻译：具有增强的合成活性的突变型神经酰胺糖内切酶
公开(公告)号：US09068208B2
公开(公告)日：2015-06-30
申请号：US13950259
申请日：2013-07-24
申请人： Karl F. Johnson , Shawn Defrees , Stephen Withers , Mark Vaughan
发明人： Karl F. Johnson , Shawn Defrees , Stephen Withers , Mark Vaughan
IPC分类号： C12P21/06 , C12P19/00 , C12N9/24 , C12N1/20 , C12P19/26
CPC分类号： C12P19/26 , C12N9/2402 , C12Y302/01123
摘要： The present invention relates to a novel endoglycoceramidase whose hydrolytic activity has been substantially reduced or eliminated, such that the enzyme is useful for synthesis of glycolipids from a monosaccharide or oligosaccharide and a ceramide. More specifically, the endoglycoceramidase is a mutant version of a naturally occurring endoglycoceramidase, preferably comprising a mutation within the active site or the nucleophilic site of the enzyme and more preferably comprising a substitution mutation of the Glu residue within the active site or the nucleophilic site. Also disclosed are a method for generating the mutant endoglycoceramidase and a method for enzymatically synthesizing glycolipids using this mutant enzyme.
摘要翻译：本发明涉及其水解活性已经显着降低或消除的新型神经酰胺糖内切酶，使得该酶可用于从单糖或寡糖和神经酰胺合成糖脂。更具体地，神经酰胺糖内切酶是天然存在的神经酰胺糖内切酶的突变形式，优选地包含酶的活性位点或亲核位点内的突变，更优选地包含活性位点或亲核位点内的Glu残基的取代突变。还公开了产生突变型神经酰胺糖内切酶的方法和使用该突变酶酶促合成糖脂的方法。

32. 发明申请

US20150031085A1 Novel Leech Hyaluronidase and Its Application 有权
标题翻译：新型水蛭透明质酸酶及其应用
公开(公告)号：US20150031085A1
公开(公告)日：2015-01-29
申请号：US14251579
申请日：2014-04-12
申请人： Jian Chen , Guocheng Du , Zhen Kang , Peng Jin
发明人： Jian Chen , Guocheng Du , Zhen Kang , Peng Jin
IPC分类号： C12N9/24 , C12P19/26
CPC分类号： C12N9/2402 , C12P19/26 , C12P21/02 , C12Y302/01036
摘要： The present invention provides a novel leech HAase and a method of producing low-molecular-weight HA oligosaccharides using the leech HAase. This invention successfully cloned the first leech HAase gene and provides a method for high-level expression of the leech HAase gene. By controlling the incubation condition, different HA oligosaccharides, particularly HA4, HA6, HA8 and HA10, can be selectively generated using the leech HAase. The large-scale expression of the leech HAase and the enzymatic production of specific HA oligosaccharides are not only useful for the cosmetic, healthcare and the medical industries but also can be a great help to polysaccharides chemical synthesis and cancer research.
摘要翻译：本发明提供了一种新的水蛭HAase和使用该水蛭HAase生产低分子量HA寡糖的方法。本发明成功克隆了第一个水蛭HAase基因，提供了一种高水平表达水蛭HAase基因的方法。通过控制孵育条件，可以使用水蛭HAase选择性地产生不同的HA寡糖，特别是HA4，HA6，HA8和HA10。水蛭HAase的大规模表达和特异性HA寡糖的酶促生产不仅可用于化妆品，保健和医疗行业，而且可以有助于多糖化学合成和癌症研究。

33. 发明申请

US20140356912A1 CELL-BASED PRODUCTION OF NONULOSONATES 有权
标题翻译：基于细胞的非线性生产
公开(公告)号：US20140356912A1
公开(公告)日：2014-12-04
申请号：US14444622
申请日：2014-07-28
申请人： National Research Council of Canada , University of Ottawa
发明人： Christopher N. Boddy , Susan M. Logan , Benjamin R. Lundgren , Ian C. Schoenhofen , Dennis M. Whitfield
IPC分类号： C12P19/26 , C12P19/30 , C12N15/70
CPC分类号： C12P19/26 , C12N15/52 , C12N15/70 , C12P19/305
摘要： The present invention relates to the the cell-based production of bacterial nonulosonates and their biosynthetic precursors. Specifically, the present invention provides recombinant cells for the production of pseudaminic acid, legionaminic acid, UDP-2,4-diacetamido-2,4,6-trideoxy-□-L-altropyranose, and UDP-2,4-diacetamido-2,4,6-trideoxy-□-D-glucopyranose. Methods for producing the sugars are also provided.
摘要翻译：本发明涉及细菌非细菌的细胞生产及其生物合成前体。具体地说，本发明提供了用于制备假胺酸，军团氨酸，UDP-2,4-二乙酰氨基-2,4,6-三脱氧-D-吡喃糖的重组细胞和UDP-2,4-二乙酰氨基-2 ，4,6-三脱氧-D-吡喃葡萄糖。还提供了生产糖的方法。

34. 发明申请

US20140342037A1 NOVEL PREBIOTICS 审中-公开
标题翻译：新的预兆
公开(公告)号：US20140342037A1
公开(公告)日：2014-11-20
申请号：US14317295
申请日：2014-06-27
申请人： DSM IP ASSETS B.V.
发明人： Albertus Alard VAN DIJK , Yulia M. EFIMOVA , Margot Elisabeth Francoise SCHOONEVELD-BERGMANS
IPC分类号： A23L1/30
CPC分类号： A23L31/00 , A23C9/1216 , A23C9/15 , A23C21/02 , A23K20/121 , A23K20/163 , A23L5/00 , A23L29/30 , A23L33/10 , A23L33/125 , A23L33/21 , A23V2002/00 , C07H7/027 , C12P19/04 , C12P19/26
摘要： The present invention relates to a composition which comprises: an oligosaccharide which is free of sialyloligosaccharide, and free sialic acid.
摘要翻译：本发明涉及一种组合物，其包含：不含唾液酸寡糖的寡糖和游离唾液酸。

35. 发明授权

US08883452B2 K5 heparosan fermentation and purification 有权
标题翻译： K5肝炎发酵和纯化
公开(公告)号：US08883452B2
公开(公告)日：2014-11-11
申请号：US13261193
申请日：2010-08-30
申请人： Zhenyu Wang , Robert J. Linhardt , Jonathan S. Dordick , Ujjwal Bhaskar
发明人： Zhenyu Wang , Robert J. Linhardt , Jonathan S. Dordick , Ujjwal Bhaskar
IPC分类号： C08B37/10 , C12P19/28 , C08B37/00 , C12P19/26
CPC分类号： C08B37/0063 , C08B37/0003 , C12P19/26
摘要： A method for the production of heparosan from fermentation culture of E. coli K5 suitable for industrial production, exhibiting superior yield and purity, smaller culture volumes, faster growth, and lower costs.
摘要翻译：一种适用于工业生产的大肠杆菌K5发酵培养生产肝素的方法，具有优异的产量和纯度，较小的培养体积，更快的生长和更低的成本。

36. 发明申请

US20140273176A1 Systems and Methods for Utilizing and Recovering Chitosan to Process Biological Material 有权
标题翻译：利用和回收壳聚糖处理生物材料的系统和方法
公开(公告)号：US20140273176A1
公开(公告)日：2014-09-18
申请号：US13839647
申请日：2013-03-15
申请人： Daniel Fleischer
发明人： Daniel Fleischer
IPC分类号： C12N1/02 , C08B37/08
CPC分类号： C08B37/003 , C08B37/0003 , C12M47/02 , C12P19/04 , C12P19/26
摘要： Systems and methods for processing biological material utilizing chitosan are provided herein. Methods may include the steps of reducing the pH of an amount of culture to about 5 to 7, incorporating an amount of a chitosan solution into the culture to form a mixture, wherein chitosan in the chitosan solution binds to the biological material; increasing the pH of the culture to about 7 to 10, to flocculate biological material in the culture, concentrating the flocculates, reducing the pH of the concentrated flocculates to about 3 to 5, and separating the recovered chitosan solution from the concentrated biological material.
摘要翻译：本文提供了利用壳聚糖处理生物材料的系统和方法。方法可以包括以下步骤：将培养基的pH降低至约5至7，向培养物中加入一定量的壳聚糖溶液以形成混合物，其中壳聚糖溶液中的壳聚糖结合生物材料; 将培养物的pH增加至约7〜10，使培养物中的生物材料絮凝，浓缩絮凝物，将浓缩的絮凝物的pH降低至约3至5，并将回收的壳聚糖溶液与浓缩的生物材料分离。

37. 发明申请

US20140134679A1 METHOD FOR PRODUCING POLYSACCHARIDE 有权
公开(公告)号：US20140134679A1
公开(公告)日：2014-05-15
申请号：US14123291
申请日：2012-05-31
申请人： Fusamitsu Yanagihara , Kentaro Matsuo , Miki Kusano
发明人： Fusamitsu Yanagihara , Kentaro Matsuo , Miki Kusano
IPC分类号： C12P19/04
CPC分类号： C12P19/04 , C12P19/26
摘要： The present invention aims to provide a method for producing a polysaccharide with high efficiency using a polysaccharide synthase. The present invention provides a method for producing a polysaccharide, including allowing polysaccharide synthase (B) to act on ribonucleoside diphosphate-monosaccharide (A) shown below to produce a polysaccharide, wherein in 10 to 100% of the duration in which (B) acts on (A), the concentration of ribonucleoside diphosphate in a reaction solution is lower than 100 times an inhibitory concentration IC50 described below against polysaccharide synthase (B).Inhibitory concentration IC50: a concentration of ribonucleoside diphosphate at which an enzyme activity of polysaccharide synthase (B) is reduced by half while under a condition where (B) has a concentration at which (B) acts on ribonucleoside diphosphate-monosaccharide (A), wherein ribonucleoside diphosphate-monosaccharide (A) is used as a substrate and ribonucleoside diphosphate is used as an inhibitor.Ribonucleoside diphosphate-monosaccharide (A): a sugar nucleotide in which a proton of at least one hydroxyl group of at least one monosaccharide (a) selected from the group consisting of triose (a-1), tetrose (a-2), pentose (a-3), hexose (a-4), heptose (a-5), and monosaccharide (a-6) described below is substituted with a functional group represented by any one of chemical formulae (1) to (5) below.Monosaccharide (a-6): a monosaccharide selected from the group consisting of (a-1), (a-2), (a-3), (a-4), and (a-5), in which at least one member selected from the group consisting of a proton, a hydroxyl group, and a hydroxymethyl group of the monosaccharide is substituted with substituent (E) described below.Substituent (E) is at least one substituent selected from the group consisting of carboxyl, amino, N-acetylamino, sulfate, methylester, N-glycolyl, methyl, 1,2,3-trihydroxypropyl, phosphate, and 2-carboxy-2-hydroxyethyl groups.

38. 发明申请

US20140127750A1 FAGOPYRITOL SYNTHASE GENES AND USES THEREOF 审中-公开
标题翻译： FAGOPYRITOL SYNTHASE基因及其用途
公开(公告)号：US20140127750A1
公开(公告)日：2014-05-08
申请号：US14104788
申请日：2013-12-12
申请人： CORNELL UNIVERSITY
发明人： Ralph L. OBENDORF , Takashi UEDA
IPC分类号： C12N9/10 , C12P19/46
CPC分类号： C12P19/46 , C07H21/04 , C12N9/1051 , C12N15/8245 , C12P19/26 , Y02P20/52
摘要： The present invention relates to an isolated DNA molecule encoding a fagopyritol synthase. A method for producing a fagopyritol, an insulin mediator, an insulin mediator analogue, an insulin mediator homologue, or an insulin mediator inhibitor is also described. The method includes providing a fagopyritol synthase, providing a substrate comprising a galactosyl donor and a galactosyl acceptor, and combining the fagopyritol synthase with the substrate under conditions effective produce a fagopyritol, an insulin mediator, an insulin mediator analogue, an insulin mediator homologue, or an insulin mediator inhibitor.
摘要翻译：本发明涉及编码吡喃葡萄糖合酶的分离的DNA分子。还描述了生产吡啶并吡喃糖醇，胰岛素介体，胰岛素介体类似物，胰岛素介体同系物或胰岛素介导抑制剂的方法。所述方法包括提供假吡格列酮合成酶，提供包含半乳糖供体和半乳糖基受体的底物，以及在有效产生假吡格列醇，胰岛素介体，胰岛素介体类似物，胰岛素介体同系物或胰岛素介导抑制剂。

39. 发明申请

US20140080201A1 Biosynthesis of Human Milk Oligosaccharides in Engineered Bacteria 有权
标题翻译：人乳寡糖在工程细菌中的生物合成
公开(公告)号：US20140080201A1
公开(公告)日：2014-03-20
申请号：US14033664
申请日：2013-09-23
申请人： GLYCOSYN LLC
发明人： Massimo Merighi , John M. McCoy , Matthew Ian Heidtman
IPC分类号： C12N15/70
CPC分类号： C12N15/70 , C07H3/06 , C07H13/04 , C12N9/00 , C12N9/1051 , C12N9/2471 , C12P19/00 , C12P19/18 , C12P19/26 , Y02P20/52
摘要： The invention provides compositions and methods for engineering bacteria to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection.
摘要翻译：本发明提供用于工程细菌生产岩藻糖基化寡糖的组合物和方法及其在预防或治疗感染中的用途。

40. 发明申请

US20140066617A1 METHOD FOR PRODUCING SIALIC-ACID-CONTAINING SUGAR CHAIN 有权
标题翻译：生产含酸性糖的糖链的方法
公开(公告)号：US20140066617A1
公开(公告)日：2014-03-06
申请号：US14002645
申请日：2012-02-27
申请人： Yasunori Chiba , Yoshie Takahashi , Hisashi Narimatsu , Kazuhiro Fukae
发明人： Yasunori Chiba , Yoshie Takahashi , Hisashi Narimatsu , Kazuhiro Fukae
IPC分类号： C12P19/18 , C08B37/00
CPC分类号： C08B37/0063 , C08B37/006 , C12N9/1081 , C12N15/09 , C12N15/52 , C12P19/04 , C12P19/18 , C12P19/26 , C12P21/005 , C12Y204/99 , C12Y204/99001 , C12Y301/03
摘要： [Problem to be Solved]The importance of sugar chains having α2,3- or α2,6-linked sialic acid at their non-reducing ends is known. Industrial production has been demanded for these sugar chain compounds. Particularly, the production of glycoprotein drugs or the like inevitably requires producing in quantity sugar chains having homogeneous structures by controlling the linking pattern (α2,6-linkage or α2,3-linkage) of sialic acid. Particularly, a triantennary or tetraantennary N-type complex sugar chain having sialic acid at each of all non-reducing ends is generally considered difficult to chemically synthesize. There has been no report disclosing that such a sugar chain was chemically synthesized. Furthermore, these sugar chains are also difficult to efficiently prepare enzymatically.[Solution]The present inventors have newly found the activity of sialyltransferase of degrading sialic acid on a reaction product in the presence of CMP and also found that formed CMP can be degraded enzymatically to thereby efficiently produce a sialic acid-containing sugar chain. The present inventors have further found that even a tetraantennary N-type sugar chain having four α2,6-linked sialic acid molecules, which has previously been difficult to synthesize, can be prepared at high yields by one-pot synthesis comprising the elongation reaction of a biantennary sugar chain used as a starting material without performing purification after each enzymatic reaction.
摘要翻译： [待解决的问题]已知具有α2,3-或α2,6-连接的唾液酸在其非还原末端的糖链的重要性。这些糖链化合物已经需要工业生产。特别地，糖蛋白药物等的生产不可避免地需要通过控制唾液酸的连接模式（α2,6-键或α2,3-键）来产生具有均匀结构的量的糖链。特别地，在所有非还原性末端中的每一个具有唾液酸的三末端或四末端N-型复合糖链通常被认为难以化学合成。没有报道披露这样的糖链是化学合成的。此外，这些糖链也难以有效地制备酶。 [解决方案]本发明人在CMP存在下新发现唾液酸转移酶唾液酸转移酶在反应产物上的活性，并且发现形成的CMP可以酶促降解，从而有效地产生含唾液酸的糖链。本发明人进一步发现，即使具有四个α2,6-连接的唾液酸分子的四末端N-型糖链，其先前难以合成，可以通过一锅合成以高收率制备，其包括延伸反应在每个酶反应后不用进行纯化的双天线糖链用作原料。

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