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31. 发明申请

US20120034634A1 MODIFIED LUCIOLA CRUCIATA LUCIFERASE PROTEIN 有权
标题翻译：改良的LUCIOLA CRUCIATA LUCIFERASE蛋白
公开(公告)号：US20120034634A1
公开(公告)日：2012-02-09
申请号：US12800830
申请日：2010-05-24
申请人： Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
发明人： Daniel J. Coleman , John J. Naleway , Gabriele M. Cook
IPC分类号： C12N9/02 , C07H21/04 , C12Q1/66
CPC分类号： C12N15/85 , C07K14/43577 , C12N9/0069 , C12N2800/107
摘要： A codon optimized and stabilized luciferase gene based upon the sequence of the natural luciferase gene isolated from Luciola cruciata (Japanese firefly) and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase.
摘要翻译：基于从Luciola cruciata（日本萤火虫）分离的天然荧光素酶基因的序列的密码子优化和稳定的荧光素酶基因和新型重组DNA，其特征在于将编码新的荧光素酶的该新基因并入载体DNA中以改善哺乳动物细胞的活性，被披露。与天然荧光素酶相比，这种新的荧光素酶表现出长波长发光，以及哺乳动物细胞系统中改善的热稳定性和更高的表达水平。

32. 发明授权

US08105827B2 Protein expression systems 有权
标题翻译：蛋白表达系统
公开(公告)号：US08105827B2
公开(公告)日：2012-01-31
申请号：US11851042
申请日：2007-09-06
申请人： Yu-Chan Chao , Catherine Y. Y. Liu , Carol P. Y. Wu
发明人： Yu-Chan Chao , Catherine Y. Y. Liu , Carol P. Y. Wu
IPC分类号： C12N15/85 , C12N15/86 , C12N1/21 , C12N1/15 , C12N5/14 , C12N1/19
CPC分类号： C12N15/85 , C12N2800/107 , C12N2830/00
摘要： Disclosed are recombinant non-insect host cells and use of activation of CMV or SV40 promoters by IE1, IE2 and the enhancer hr of the baculovirus in non-insect host cells for expression heterologous RNAs or polypeptides. Also disclosed are methods of using the cells.
摘要翻译：公开了重组非昆虫宿主细胞，并且使用IE6，IE2中的CMV或SV40启动子和非昆虫宿主细胞中杆状病毒的增强子hr用于表达异源RNA或多肽的活化。还公开了使用细胞的方法。

33. 发明申请

US20110250232A1 DNA-Transfection System for the Generation of Infectious Influenza Virus 有权
标题翻译：用于传染性流感病毒生成的DNA转染系统
公开(公告)号：US20110250232A1
公开(公告)日：2011-10-13
申请号：US13150426
申请日：2011-06-01
申请人： Erich HOFFMANN
发明人： Erich HOFFMANN
IPC分类号： A61K39/145 , A61P31/16 , A61P31/14 , A61K39/12 , A61P37/04
CPC分类号： C07K14/005 , A61K2039/5252 , A61K2039/5254 , C12N7/00 , C12N2720/00063 , C12N2760/16122 , C12N2760/16151 , C12N2760/16163 , C12N2760/16222 , C12N2760/16263 , C12N2760/18663 , C12N2770/24263 , C12N2800/107 , C12N2800/40 , C12N2830/205 , C12N2830/34 , C12N2830/36 , C12N2830/85
摘要： The present invention is based on the development of a dual promoter system (preferably a RNA pol I-pol II system) for the efficient intracellular synthesis of viral RNA. The resultant minimal plasmid-based system may be used to synthesize any RNA virus, preferably viruses with a negative single stranded RNA genome. The viral product of the system is produced when the plasmids of the system are introduced into a suitable host cell. One application of the system is production of attenuated, reassortant influenza viruses for use as antigens in vaccines. The reassortant viruses generated by cotransfection of plasmids may comprise genes encoding the surface glycoproteins hemagglutinin and neuraminidase from an influenza virus currently infecting the population and the internal genes from an attenuated influenza virus. An advantageous property of the present invention is its versatility; the system may be quickly and easily adapted to synthesize an attenuated version of any RNA virus. Attenuated or inactivated RNA viruses produced by the present invention may be administered to a patient in need of vaccination by any of several routes including intranasally or intramuscularly.
摘要翻译：本发明基于用于病毒RNA的有效细胞内合成的双重启动子系统（优选RNA pol I-pol II系统）的开发。所得到的基于最小质粒的系统可用于合成任何RNA病毒，优选具有负单链RNA基因组的病毒。当将系统的质粒引入合适的宿主细胞时，产生系统的病毒产物。该系统的一个应用是生产用作疫苗抗原的减毒重配流感病毒。通过共转染质粒产生的重配病毒可以包含编码来自目前感染群体的流感病毒的表面糖蛋白血凝素和神经氨酸酶的基因以及来自减毒流感病毒的内部基因。本发明的优点是其多功能性; 该系统可以快速且容易地适应于合成任何RNA病毒的减毒版本。通过本发明产生的减毒或灭活的RNA病毒可以通过包括鼻内或肌肉内的几种途径中的任何途径施用于需要接种疫苗的患者。

34. 发明申请

US20110162096A1 Gene Therapy Using Transposon-Based Vectors 有权
标题翻译：使用基于转座子的载体的基因治疗
公开(公告)号：US20110162096A1
公开(公告)日：2011-06-30
申请号：US12941448
申请日：2010-11-08
申请人： Richard Cooper , Frederick M. Enright , William C. Fioretti
发明人： Richard Cooper , Frederick M. Enright , William C. Fioretti
IPC分类号： A01K67/027 , C12N5/071 , A61K48/00 , A01K67/00 , A61P35/00 , A61P15/08 , A23C9/00
CPC分类号： C12N15/8509 , A61K48/00 , C12N2800/107 , C12N2800/90
摘要： Methods and compositions are presented for the administration of transposon-based vectors to an animal or human to provide gene therapy to the animal or human
摘要翻译：呈现方法和组合物，用于向动物或人施用基于转座子的载体，以向动物或人提供基因治疗

35. 发明申请

US20110105593A1 MicroRNA-Formatted Multitarget Interfering RNA Vector Constructs and Methods of Using The Same 审中-公开
标题翻译：微RNA格式化多靶点干扰RNA载体构建体及其使用方法
公开(公告)号：US20110105593A1
公开(公告)日：2011-05-05
申请号：US12793248
申请日：2010-06-03
申请人： Laura Steel , Catherine J. Pachuk
发明人： Laura Steel , Catherine J. Pachuk
IPC分类号： A61K31/7088 , C12N15/63 , C12N15/85 , A61P31/20 , A61P31/14
CPC分类号： C12N15/85 , C12N15/111 , C12N2310/111 , C12N2310/14 , C12N2310/51 , C12N2730/10022 , C12N2800/107 , C12N2830/008 , C12N2830/20
摘要： Vectors expressing multiple microRNA (miRNA)-formatted interfering RNAs from a single transcript are disclosed and methods of using the same to inhibit expression of one or more target genes.
摘要翻译：公开了表达来自单个转录物的多种微小RNA（miRNA）形成的干扰RNA的载体和使用其从抑制一种或多种靶基因表达的方法。

36. 发明授权

US07811580B2 Chimeric HIV Env proteins comprising CD4 mini-proteins or CD4 mimetics that are capable of inducing neutralizing antibody responses against cryptic Env epitopes 有权
标题翻译：包含能够诱导针对隐性Env表位的中和抗体应答的CD4微型蛋白或CD4模拟物的嵌合HIV Env蛋白
公开(公告)号：US07811580B2
公开(公告)日：2010-10-12
申请号：US10514055
申请日：2003-05-07
申请人： Susan Barnett , Indresh Srivastava
发明人： Susan Barnett , Indresh Srivastava
IPC分类号： A61K39/00 , A61K39/21 , C07H21/04 , C12N5/00 , C12N15/00
CPC分类号： C07K14/005 , A61K39/00 , A61K39/12 , A61K39/21 , A61K2039/53 , A61K2039/545 , A61K2039/55566 , A61K2039/6031 , C07K14/70514 , C07K16/1063 , C07K16/2812 , C07K2317/32 , C07K2317/76 , C07K2319/32 , C12N2740/10043 , C12N2740/16122 , C12N2740/16134 , C12N2770/36143 , C12N2800/107
摘要： Env-CD4 complexes and hybrids are disclosed that expose cryptic epitopes that are important in virus neutralization. Methods of diagnosis, treatment and prevention using the polynucleotides and polypeptides are also provided.
摘要翻译：公开了Env-CD4复合物和杂交体，其暴露在病毒中和中重要的神经表位。还提供了使用多核苷酸和多肽进行诊断，治疗和预防的方法。

37. 发明申请

US20100203509A1 Inducible fluorescently-tagged protein expression system 审中-公开
标题翻译：诱导荧光标记蛋白表达系统
公开(公告)号：US20100203509A1
公开(公告)日：2010-08-12
申请号：US12228905
申请日：2008-08-18
申请人： Allal Ouhit , Zakaria Abdel Mageed , Mourad Zerfaoui
发明人： Allal Ouhit , Zakaria Abdel Mageed , Mourad Zerfaoui
IPC分类号： C12Q1/68 , C07H21/04 , C12N15/63 , C12N1/21 , C12P21/00
CPC分类号： C12P21/02 , C07K14/43595 , C07K14/70585 , C07K14/70596 , C07K2319/60 , C12N15/85 , C12N2800/107 , C12N2830/003 , C12N2830/006 , C12N2830/15
摘要： The present invention is related to an antibiotic inducible/repressible genetic construct for controlling transcription of a gene of interest in a cell, and methods for its use. The genetic construct provides a plasmid vector comprising a polynucleotide molecule, two tetO sequences, and a single CMV promoter, wherein the polynucleotide molecule further comprises a fluorescent protein gene and an MCS upstream of the fluorescent protein gene, wherein a gene of interest encoding a protein of interest is cloned into the MCS so that a fusion protein comprising the protein of interest and the fluorescent protein may be produced, the polynucleotide molecule being operably linked to two tetO sequences and the single CMV promoter, and wherein the two tetO sequences are incorporated into the single CMV promoter.
摘要翻译：本发明涉及用于控制细胞中感兴趣的基因的转录的抗生素诱导/抑制遗传构建体及其使用方法。遗传构建物提供包含多核苷酸分子，两个tetO序列和单个CMV启动子的质粒载体，其中多核苷酸分子还包含荧光蛋白基因和荧光蛋白基因上游的MCS，其中编码蛋白质的目标基因感兴趣的克隆到MCS中，使得可以产生包含目的蛋白质和荧光蛋白的融合蛋白，所述多核苷酸分子可操作地连接到两个tetO序列和单个CMV启动子，并且其中将两个tetO序列并入单个CMV启动子。

38. 发明申请

US20090028825A1 Compositions and Methods for Studying and Treating Inflammatory Diseases and Disorders 失效
标题翻译：研究和治疗炎症疾病和疾病的组合和方法
公开(公告)号：US20090028825A1
公开(公告)日：2009-01-29
申请号：US11795789
申请日：2006-01-20
申请人： Stephanos Kyrkanides
发明人： Stephanos Kyrkanides
IPC分类号： A61K35/76 , C12N15/63 , A61P19/02 , A61P25/28 , A61P29/00 , C12N5/00
CPC分类号： C12N15/86 , A61K48/005 , A61K48/0058 , C07K2319/02 , C12N15/111 , C12N15/113 , C12N15/1137 , C12N2310/111 , C12N2310/14 , C12N2320/50 , C12N2330/30 , C12N2740/15043 , C12N2740/15045 , C12N2800/107 , C12N2800/30 , C12N2830/002 , C12N2830/008 , C12N2840/203
摘要： Disclosed are compositions and methods that can be used in the treatment of diseases and disorders caused, exacerbated or otherwise affected by inflammation.
摘要翻译：公开了可用于治疗引起，加重或以其他方式受炎症影响的疾病和病症的组合物和方法。

39. 发明申请

US20080152689A1 DEVICES CONTAINING DNA ENCODING NEUROTROPHIC AGENTS AND RELATED COMPOSITIONS AND METHODS 审中-公开
标题翻译：含有编码神经营养因子的DNA的装置及相关组合物和方法
公开(公告)号：US20080152689A1
公开(公告)日：2008-06-26
申请号：US11951970
申请日：2007-12-06
申请人： Andrew Baird , Ana Maria Gonzalez , Martin Berry , Ann Logan
发明人： Andrew Baird , Ana Maria Gonzalez , Martin Berry , Ann Logan
IPC分类号： A61K9/00 , A61K35/12 , A61P25/00
CPC分类号： A61K38/179 , A61K9/0024 , A61K35/15 , A61K35/30 , A61K38/00 , A61K38/1709 , A61K38/1841 , A61K38/185 , A61K41/0042 , A61K47/42 , A61K47/642 , A61K47/6425 , A61K48/00 , A61K48/0008 , A61K48/0058 , A61K48/0075 , C07K14/415 , C07K14/475 , C07K14/503 , C07K14/52 , C07K2319/00 , C07K2319/03 , C07K2319/04 , C07K2319/09 , C07K2319/21 , C07K2319/50 , C07K2319/55 , C07K2319/75 , C07K2319/80 , C12N15/62 , C12N15/85 , C12N15/87 , C12N2799/026 , C12N2800/107 , C12N2810/851 , C12N2830/008 , Y10S977/912 , Y10S977/927
摘要： Devices useful in the delivery of DNA encoding neurotrophic agents, anti-fibrotic agents, and related compositions are disclosed herein for use in the treatment of central and/or peripheral nervous system injury. Methods of making and using the disclosed devices and DNA are also described. In various embodiments, the invention also discloses compositions and devices that may further include a targeting agent, such as a polypeptide that is reactive with an FGF receptor (e.g., bFGF), or another ligand that binds to cell surface receptors on neuronal cells, or a support cell. The invention also discloses methods of promoting neuronal survival and regeneration via transfection of an axon as it grows through a device or composition of the present invention, or via transfection of a repair cell.
摘要翻译：用于递送编码神经营养剂，抗纤维化剂和相关组合物的DNA的装置在本文中公开用于治疗中枢和/或周围神经系统损伤。还描述了制造和使用所公开的装置和DNA的方法。在各种实施方案中，本发明还公开了可以进一步包括靶向剂，例如与FGF受体（例如bFGF）反应的多肽或与神经元细胞上的细胞表面受体结合的另一种配体的组合物和装置，或支撑细胞本发明还公开了当轴突通过本发明的装置或组合物生长或通过转染修复细胞时转染轴突促进神经元存活和再生的方法。

40. 发明授权

US07312064B2 DNA transfection system for the generation of infectious influenza virus 有权
标题翻译：用于产生感染性流感病毒的DNA转染系统
公开(公告)号：US07312064B2
公开(公告)日：2007-12-25
申请号：US11093430
申请日：2005-03-29
申请人： Erich Hoffmann
发明人： Erich Hoffmann
IPC分类号： C12N7/00
CPC分类号： C07K14/005 , A61K2039/5252 , A61K2039/5254 , C12N7/00 , C12N2720/00063 , C12N2760/16122 , C12N2760/16151 , C12N2760/16163 , C12N2760/16222 , C12N2760/16263 , C12N2760/18663 , C12N2770/24263 , C12N2800/107 , C12N2800/40 , C12N2830/205 , C12N2830/34 , C12N2830/36 , C12N2830/85
摘要： The present invention is based on the development of a dual promoter system (preferably a RNA pol I-pol II system) for the efficient intracellular synthesis of viral RNA. The resultant minimal plasmid-based system may be used to synthesize any RNA virus, preferably viruses with a negative single stranded RNA genome. The viral product of the system is produced when the plasmids of the system are introduced into a suitable host cell. One application of the system is production of attenuated, reassortant influenza viruses for use as antigens in vaccines. The reassortant viruses generated by cotransfection of plasmids may comprise genes encoding the surface glycoproteins hemagglutinin and neuraminidase from an influenza virus currently infecting the population and the internal genes from an attenuated influenza virus. An advantageous property of the present invention is its versatility; the system may be quickly and easily adapted to synthesize an attenuated version of any RNA virus. Attenuated or inactivated RNA viruses produced by the present invention may be administered to a patient in need of vaccination by any of several routes including intranasally or intramuscularly.
摘要翻译：本发明基于用于病毒RNA的有效细胞内合成的双重启动子系统（优选RNA pol I-pol II系统）的开发。所得到的基于最小质粒的系统可用于合成任何RNA病毒，优选具有负单链RNA基因组的病毒。当将系统的质粒引入合适的宿主细胞时，产生系统的病毒产物。该系统的一个应用是生产用作疫苗抗原的减毒重配流感病毒。通过共转染质粒产生的重配病毒可以包含编码来自目前感染群体的流感病毒的表面糖蛋白血凝素和神经氨酸酶的基因以及来自减毒流感病毒的内部基因。本发明的优点是其多功能性; 该系统可以快速且容易地适应于合成任何RNA病毒的减毒版本。通过本发明产生的减毒或灭活的RNA病毒可以通过包括鼻内或肌肉内的几种途径中的任何途径施用于需要接种疫苗的患者。

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