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31. 发明申请

US20060014934A1 Crystal structure of factor Vai and method for identifying blood factor Va modulators 审中-公开
标题翻译：因子Vai的晶体结构和鉴定血液因子Va调节剂的方法
公开(公告)号：US20060014934A1
公开(公告)日：2006-01-19
申请号：US11131091
申请日：2005-05-17
申请人： Stephen Everse , Ty Adams , Matthew Hockin , Kenneth Mann
发明人： Stephen Everse , Ty Adams , Matthew Hockin , Kenneth Mann
IPC分类号： C07K14/745 , G01N33/48 , A61K38/48
CPC分类号： C12Q1/56 , A61K38/00 , C07K14/745 , C12N2517/00 , G01N33/86 , G01N2333/7456 , G01N2500/00
摘要： The present invention shows the crystal structure of protein C inactivated factor Va (A1-A3-C1-C2) that depicts a novel domain arrangement. The newly disclosed orientation has implications for binding to membranes essential for function. A high-affinity calcium binding site and a copper binding site have been identified, neither of which show a direct involvement in chain association. This structure represents the largest physiologically relevant fragment of factor Va solved to date and provides a new scaffold for generation of models of coagulation factors.
摘要翻译：本发明示出了描绘新颖结构排列的蛋白C失活因子Va（A 1 -A 3 -C 1 -C 2）的晶体结构。新公开的方向对结合功能必不可少的膜有影响。已经鉴定了高亲和力钙结合位点和铜结合位点，这两者都不直接参与链结合。该结构代表迄今为止解决的因子Va的最大的生理相关片段，并提供用于产生凝血因子模型的新支架。

32. 发明授权

US06960434B2 Methods for assaying gene imprinting and methylated CpG islands 失效
公开(公告)号：US06960434B2
公开(公告)日：2005-11-01
申请号：US09861893
申请日：2001-05-22
申请人： Andrew P. Feinberg , Liora Strichman-Almashanu , Shan Jiang
发明人： Andrew P. Feinberg , Liora Strichman-Almashanu , Shan Jiang
IPC分类号： A01K67/027 , A61P35/00 , C12N5/074 , C12N5/10 , C12N15/09 , C12Q1/02 , C12Q1/68 , C12P19/34
CPC分类号： C12N5/0611 , C12N2501/115 , C12N2501/125 , C12N2501/235 , C12N2501/385 , C12N2502/13 , C12N2503/00 , C12N2503/02 , C12N2510/00 , C12N2517/00 , C12Q1/6827 , C12Q1/6886 , C12Q2600/118 , C12Q2600/136 , C12Q2600/154
摘要： Genomic imprinting is a parent of origin-dependent gene silencing that involves marking of alleles in the germline and differential expression in somatic cells of the offspring. Imprinted genes and abnormal imprinting have been implicated in development, human disease, and embryonic stem cell transplantation. We have established a model system for genomic imprinting using pluripotent 8.5 d.p.c. mouse embryonic germ (EG) cell lines derived from an interspecific cross. We find that allele-specific imprinted gene expression has been lost in these cells. However, partial restoration of allele-specific silencing can occur for some imprinted genes after in vitro differentiation of EG cells into somatic cell lineages, indicating the presence of a gametic memory that is separable from allele-specific gene silencing. We have also generated a library containing most methylated CpG islands. A subset of these clones was analyzed and revealed a subdivision of methylated CpG islands into 4 distinct subtypes: CpG islands belonging to high copy number repeat families; unique CpG islands methylated in all tissues; unique methylated CpG islands that are unmethylated in the paternal germline; and unique CpG islands methylated in tumors. This approach identifies a methylome of methylated CpG islands throughout the genome.

33. 发明申请

US20020174449A1 Method for generating cloned animals using chromosome shuffling 审中-公开
标题翻译：使用染色体洗牌产生克隆动物的方法
公开(公告)号：US20020174449A1
公开(公告)日：2002-11-21
申请号：US10103806
申请日：2002-03-25
发明人： Michael West , Jose Cibelli
IPC分类号： A01K067/027
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/101 , C12N15/873 , C12N15/877 , C12N15/8771 , C12N2517/00 , C12N2517/04
摘要： The present invention concerns the use of chromosomal replacement techniques in the context of producing cloned and transgenic animals, in order to correct chromosome abnormalities or alter autosomal genotypes, and provide for novel breeding pairs by replacing the sex chromosome in animals to be cloned. Replacement of a sex chromosome, or an X or Y chromosome, will result in animals that are autosomally isogenic and sexually non-isogenic (AISN), with nullautosomally isogenicnull meaning that the paired sets of autosomes (non-sex chromosomes) in each animal are isogenic or identical. Also included in the invention are animals that are both nullautosomallynull and nullallelicallynull isogenic whereby each particular pair of chromosomes is internally isogenic or identical within a single animal as well as between animals. Such animals are particularly useful in generating a line of cloned mammals using sexual reproduction, without having to undergo nuclear transfer in order to propagate cloned animals.
摘要翻译：本发明涉及在产生克隆和转基因动物的背景下使用染色体替代技术，以便校正染色体异常或改变常染色体基因型，并通过替代待克隆的动物中的性染色体来提供新的育种对。性染色体或X或Y染色体的替代将导致具有“常染色体同源性”的常染色体同源异体和性别非同基因（AISN）的动物，这意味着每组中的成对的常染色体组（非性染色体）动物是同基因的或相同的。本发明还包括既“常染色体”和“等位性”同位素的动物，由此每个特定的一对染色体在单一动物内部以及动物之间是内生同源的或相同的。这样的动物特别可用于使用有性繁殖产生一系列克隆的哺乳动物，而不必经受核转移以繁殖克隆的动物。

34. 发明申请

US20020151054A1 Cellular production control 审中-公开
标题翻译：蜂窝生产控制
公开(公告)号：US20020151054A1
公开(公告)日：2002-10-17
申请号：US10090849
申请日：2002-03-04
发明人： Peter David Rathjen , Joy Rathjen , Kathryn May Hudson
IPC分类号： C12N005/08
CPC分类号： C12N5/0618 , A61K35/12 , C12N2501/58 , C12N2502/14 , C12N2506/02 , C12N2517/00
摘要： A method for the preparation of cells of ectodermal or endodermal lineages, of high purity which method includes providing a source of pluripotent cells; a source of a mesodermal suppression composition including cellular fibronectin (cFN); and a suitable culture medium; and culturing the pluripotent cells in the culture medium in the presence of the mesodermal suppression composition for a time sufficient to permit differentiation to ectoderm cells.
摘要翻译：用于制备高纯度的外胚层或内胚层谱系的细胞的方法，包括提供多能细胞来源; 中胚层抑制组合物的来源包括细胞纤连蛋白（cFN）; 和合适的培养基; 并在中胚层抑制组合物的存在下培养培养基中的多能细胞足以允许分化为外胚层细胞的时间。

35. 发明授权

US5625127A Extended human hematopoiesis in a heterologous host 失效
标题翻译：在异源宿主中延长人类造血功能
公开(公告)号：US5625127A
公开(公告)日：1997-04-29
申请号：US434706
申请日：1995-05-04
申请人： Hideto Kaneshima , Reiko Namikawa , Joseph M. McCune
发明人： Hideto Kaneshima , Reiko Namikawa , Joseph M. McCune
IPC分类号： A01K67/027 , C12N15/85 , A61K35/00
CPC分类号： A01K67/0271 , C12N15/8509 , A01K2217/05 , A01K2267/025 , A01K2267/0381 , C12N2517/00
摘要： A human hematopoietic system is provided in an immunocompromised mammalian host, where the hematopoietic system is functional for extended periods of time. Particularly, human fetal liver tissue and human fetal thymus is introduced into an appropriate site of a young immunocompromised mouse at a site supplied with a vascular system, whereby the fetal tissue results in novel formation of functional human bone marrow tissue.
摘要翻译：在免疫受损的哺乳动物宿主中提供人类造血系统，其中造血系统在延长的时间段内起作用。特别地，在供应有血管系统的部位将人胎肝组织和人胎儿胸腺引入幼年免疫受损小鼠的适当部位，由此胎儿组织导致功能性人骨髓组织的新形成。

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