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    • 31. 发明授权
    • Fusion proteins of bacterial luciferase as multicolor luminescent sensors
    • 细菌荧光素酶的融合蛋白作为多色发光传感器
    • US09127282B2
    • 2015-09-08
    • US13531558
    • 2012-06-24
    • Shiao-Chun Tu
    • Shiao-Chun Tu
    • C12N9/96C12N15/62
    • C12N15/62C07K2319/60C07K2319/61
    • The present invention discloses systems and methods for altering the color of bacterial bioluminescence via a fusion protein complex by fusing Discosoma sp. fluorescent protein mOrange (mOrange) with Vibrio harveyi luciferase. The fusion of mOrange to the N- or C-terminus of either α or β subunit of luciferase, via a short peptide linker produces fully active fusion enzymes. The fusion of mOrange to the N-terminus of luciferase α gives rise to a new 560-nm emission component. The same methodology may be used to alter bacterial bioluminescence color by covalent attachment of other suitable fluorescent proteins or chromophores to luciferase for generating multi-color sensors.
    • 本发明公开了通过融合蛋白复合物来改变细菌生物发光的颜色的系统和方法。 荧光蛋白mOrange(mOrange)与弧菌弧菌荧光素酶。 mOrange与α或bgr的N-或C-末端的融合; 荧光素酶的亚基通过短的肽接头产生完全活性的融合酶。 mOrange与荧光素酶α的N末端的融合产生了新的560nm发射成分。 可以使用相同的方法来通过将其它合适的荧光蛋白或发色团共价连接到荧光素酶来生成多色传感器来改变细菌生物发光颜色。
    • 32. 发明申请
    • FUSION PROTEINS OF BACTERIAL LUCIFERASE AS MULTICOLOR LUMINESCENT SENSORS
    • 细菌培养液的融合蛋白作为多光源发光传感器
    • US20130023027A1
    • 2013-01-24
    • US13531558
    • 2012-06-24
    • Shiao-Chun Tu
    • Shiao-Chun Tu
    • C12N9/96
    • C12N15/62C07K2319/60C07K2319/61
    • The present invention discloses systems and methods for altering the color of bacterial bioluminescence via a fusion protein complex by fusing Discosoma sp. fluorescent protein mOrange (mOrange) with Vibrio harveyi luciferase. The fusion of mOrange to the N- or C-terminus of either α or β subunit of luciferase, via a short peptide linker produces fully active fusion enzymes. The fusion of mOrange to the N-terminus of luciferase α gives rise to a new 560-nm emission component. The same methodology may be used to alter bacterial bioluminescence color by covalent attachment of other suitable fluorescent proteins or chromophores to luciferase for generating multi-color sensors.
    • 本发明公开了通过融合蛋白复合物来改变细菌生物发光的颜色的系统和方法。 荧光蛋白mOrange(mOrange)与弧菌弧菌荧光素酶。 mOrange与α或bgr的N-或C-末端的融合; 荧光素酶的亚基通过短的肽接头产生完全活性的融合酶。 mOrange与荧光素酶α的N末端的融合产生了新的560nm发射成分。 可以使用相同的方法来通过将其它合适的荧光蛋白或发色团共价连接到荧光素酶来生成多色传感器来改变细菌生物发光颜色。