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31. 发明授权

US08597572B2 Automatic analyzing method and apparatus for microfluidic system 失效
标题翻译：微流体系统的自动分析方法和装置
公开(公告)号：US08597572B2
公开(公告)日：2013-12-03
申请号：US12128680
申请日：2008-05-29
申请人： Su-hyeon Kim , Yoon-kyoung Cho , Jeong-gun Lee
发明人： Su-hyeon Kim , Yoon-kyoung Cho , Jeong-gun Lee
IPC分类号： G01N33/50
CPC分类号： G01N35/00732 , G01N35/00069 , G01N35/08 , G01N2035/00752 , Y10T436/11
摘要： Provided are an automatic analyzing method and apparatus for a microfludic system. The apparatus includes means for recognizing a disc or chip in the microfludic system, performs an operation and detection by automatically loading a DB according to a recognized type, and perform quantization of a test.
摘要翻译：本发明提供一种微流体系统的自动分析方法和装置。该装置包括用于识别微流体系统中的盘或芯片的装置，通过根据识别的类型自动加载DB执行操作和检测，并执行测试的量化。

32. 发明授权

US07784911B2 Apparatus and method for printing biomolecular droplet on substrate 有权
标题翻译：在基板上印刷生物分子液滴的装置和方法
公开(公告)号：US07784911B2
公开(公告)日：2010-08-31
申请号：US11747043
申请日：2007-05-10
申请人： Kui-hyun Kim , Byung-chul Kim , Kwang-ho Cheong , Su-hyeon Kim
发明人： Kui-hyun Kim , Byung-chul Kim , Kwang-ho Cheong , Su-hyeon Kim
IPC分类号： B41J2/06
CPC分类号： B01J19/0046 , B01J2219/00371 , B01J2219/00378 , B01J2219/00497 , B01J2219/00527 , B01J2219/00585 , B01J2219/00596 , B01J2219/00641 , B01J2219/00659 , B01J2219/00677 , B01J2219/00722 , B01J2219/00725 , B01J2219/00743 , B01L3/0262 , B01L2300/0819 , B01L2400/027 , C40B60/14
摘要： An apparatus and method which print a biomolecular droplet onto a solid substrate using an electric charge concentration effect comprises: a needle shaped electric field forming electrode which is made of a conductive material, is disposed vertically, and comprises an accommodating area and a nozzle formed on a bottom end of the accommodating area; a solid substrate which is electrically grounded, is disposed below the electric field forming electrode, and comprises a moisture thin film and a target surface onto which the biomolecular droplet is discharged from the nozzle of the electric field forming electrode; and an open circuit type voltage applying unit which is electrically connected to the electric field forming electrode, applies a charge to the electric field forming electrode, and causes the biomolecular droplet to be ejected onto the target surface.
摘要翻译：使用电荷浓度效应将生物分子液滴印刷到固体基质上的装置和方法包括：由导电材料制成的针形电场形成电极垂直设置，并且包括容纳区域和形成在容纳区域的底端; 电气接地的固体基板设置在电场形成电极的下方，并且包括湿气薄膜和生物分子液滴从电场形成电极的喷嘴排出的目标表面; 以及与电场形成电极电连接的开路型电压施加单元，向电场形成电极施加电荷，并使生物分子液滴喷射到目标表面上。

33. 发明授权

US07692794B2 Optical detection apparatus, optical detection method, and microfluidic system including the optical detection apparatus 有权
标题翻译：光学检测装置，光学检测方法和包括光学检测装置的微流体系统
公开(公告)号：US07692794B2
公开(公告)日：2010-04-06
申请号：US11972266
申请日：2008-01-10
申请人： Su-hyeon Kim , Jeong-gun Lee
发明人： Su-hyeon Kim , Jeong-gun Lee
IPC分类号： G01N21/25
CPC分类号： G01N21/07 , G01N21/253 , G01N21/276 , G01N21/314
摘要： Provided are an optical detection apparatus, a microfluidic system including the same, and an optical detection method. The optical detection apparatus including: at least one light emission unit which emits light of a predetermined wavelength band; at least one light receiving unit which is disposed such that the light receiving unit receives the light emitted from the light emission unit and generates an electrical signal according to the intensity of the light received, wherein the number of light receiving units is the same as the number of light emission units; a rotation operating unit which rotates a disk-type microfluidic apparatus comprising at least one detection chamber in which a sample is to be loaded such that the detection chamber is disposed in a light pathway between the light emission unit and the light receiving unit; and a processor which measures a property of the sample contained in the detection chamber using the electrical signal generated by the light receiving unit.
摘要翻译：提供了一种光学检测装置，包括该光学检测装置的微流体系统和光学检测方法。所述光学检测装置包括：发射预定波长带的光的至少一个发光单元; 至少一个光接收单元，其设置成使得所述光接收单元接收从所述发光单元发射的光并根据所接收的光的强度产生电信号，其中所述光接收单元的数量与发光单位数量; 旋转操作单元，其旋转盘式微流体装置，其包括至少一个检测室，其中样品将被装载，使得所述检测室设置在所述发光单元和所述光接收单元之间的光路径中; 以及处理器，其使用由光接收单元生成的电信号来测量包含在检测室中的样品的性质。

34. 发明申请

US20060205013A1 FET-type biosensor with surface modification 有权
标题翻译：具有表面改性的FET型生物传感器
公开(公告)号：US20060205013A1
公开(公告)日：2006-09-14
申请号：US11336110
申请日：2006-01-20
申请人： Jeo-young Shim , Su-hyeon Kim , Kyu-tae Yoo , Sung-ouk Jung , Joon-shik Park
发明人： Jeo-young Shim , Su-hyeon Kim , Kyu-tae Yoo , Sung-ouk Jung , Joon-shik Park
IPC分类号： G01N33/53
CPC分类号： B82Y30/00 , B82Y15/00 , G01N27/4145
摘要： Provided is a field effect transistor (FET) type biosensor including a source electrode, a gate, and a drain electrode. A ligand that can bind to a side of a nucleic acid is added to the surface of the gate. In a conventional FET type biosensor, it is difficult to detect a signal within the debye length because a target nucleic acid is directly fixed to the surface of a gate of the conventional FET. However, in the present invention, this problem can be overcome and the debye length can be increased by treating the surface of a gate of an FET sensor with a ligand that can bind to a side of a nucleic acid. The ligand can be adsorbed onto the surface of the gate. In this case, the nucleic acid is adsorbed parallel to the surface of the gate, not perpendicular to the surface of the gate, thus generating an effective depletion region. In addition, hybridization efficiency can be increased because a hybridized sample can be injected into an FET sensor at high ionic strength.
摘要翻译：提供了包括源电极，栅极和漏电极的场效应晶体管（FET）型生物传感器。可以结合核酸一侧的配体加入到门的表面。在传统的FET型生物传感器中，由于目标核酸直接固定在常规FET的栅极表面，难以检测德拜长度内的信号。然而，在本发明中，可以克服这个问题，并且可以通过用可以结合核酸一侧的配体处理FET传感器的栅极的表面来增加德拜长度。配体可以吸附在栅极的表面上。在这种情况下，核酸被平行于栅极的表面吸附，而不垂直于栅极的表面，从而产生有效的耗尽区域。此外，杂交效率可以提高，因为杂化样品可以以高离子强度注入FET传感器。

35. 发明申请

US20060118417A1 Nucleic acid purification method using hydrogen bonding and electric field 审中-公开
标题翻译：使用氢键和电场的核酸纯化方法
公开(公告)号：US20060118417A1
公开(公告)日：2006-06-08
申请号：US11281226
申请日：2005-11-16
申请人： Young-a Kim , Jun-hong Min , Kui-hyun Kim , Myo-yong Lee , Su-hyeon Kim , Young-nam Kwon , Jeong-gun Lee , Joon-ho Kim
发明人： Young-a Kim , Jun-hong Min , Kui-hyun Kim , Myo-yong Lee , Su-hyeon Kim , Young-nam Kwon , Jeong-gun Lee , Joon-ho Kim
IPC分类号： C07H21/04
CPC分类号： C07H21/04
摘要： Provided is a method of purifying nucleic acids using hydrogen bonding and an electric field, including: bringing a sample containing target nucleic acids into contact with an electrode coated with a material capable of forming hydrogen bonds with the target nucleic acids; applying a positive voltage to the electrode to move the target nucleic acids closer to the electrode so as to form hydrogen bonds with the material on the electrode; washing the electrode; and applying to the electrode a negative voltage to elute the bound target nucleic acids. According to the method, selectivity to nucleic acids and proteins increases due to hydrogen bonding, nucleic acid purification is possible within a short time through an electric field, and the bound nucleic acids can be efficiently eluted.
摘要翻译：提供了使用氢键和电场来净化核酸的方法，包括：使含有靶核酸的样品与涂覆有能够与靶核酸形成氢键的材料接触; 向电极施加正电压以使靶核酸更靠近电极以与电极上的材料形成氢键; 清洗电极; 并向电极施加负电压以洗脱结合的靶核酸。根据该方法，由于氢键，核酸和蛋白质的选择性增加，核酸纯化可以在短时间内通过电场进行，并且结合的核酸可以被有效地洗脱。

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