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31. 发明授权

US07255999B2 Methods and compositions for analyzing proteins 有权
标题翻译：用于分析蛋白质的方法和组合物
公开(公告)号：US07255999B2
公开(公告)日：2007-08-14
申请号：US10154042
申请日：2002-05-21
申请人： Sharat Singh , Hossein Salimi-Moosavi , Syed Hasan Tahir , Gerald J. Wallweber , Hrair Kirakossian , Tracy J. Matray , Vincent S. Hernandez
发明人： Sharat Singh , Hossein Salimi-Moosavi , Syed Hasan Tahir , Gerald J. Wallweber , Hrair Kirakossian , Tracy J. Matray , Vincent S. Hernandez
IPC分类号： G01N33/53 , G01N33/533 , C12Q1/42
CPC分类号： G01N33/6842 , C07K16/00 , C07K2317/40 , G01N33/582 , G01N33/6803
摘要： Methods, compositions and kits are disclosed for determining one or more target polypeptides in a sample where the target polypeptides have undergone a post-translational modification. A mixture comprising the sample and a first reagent comprising a cleavage-inducing moiety and a first binding agent for a binding site on a target polypeptide is subjected to conditions under which binding of respective binding moieties occurs. The binding site is the result of post-translational modification activity involving the target polypeptide. The method may be employed to determine the target polypeptide itself. In another embodiment the presence and/or amount of the target polypeptide is related to the presence and/or amount and/or activity of an agent such as an enzyme involved in the post-translational modification of the target polypeptide. The interaction between the first binding agent and the binding site brings the cleavage-inducing moiety into close proximity to a cleavable moiety, which is associated with the polypeptide and is susceptible to cleavage only when in proximity to the cleavage-inducing moiety. In this way, an electrophoretic tag for each of the polypeptides may be released. Released electrophoretic tags are separated and the presence and/or amount of the target polypeptides are determined based on the corresponding electrophoretic tags.
摘要翻译：公开了用于确定样品中一种或多种靶多肽已经经历了翻译后修饰的方法，组合物和试剂盒。包含样品和包含切割诱导部分的第一试剂和用于靶多肽上的结合位点的第一结合剂的混合物经受各自结合部分的结合发生的条件。结合位点是涉及靶多肽的翻译后修饰活性的结果。该方法可用于确定靶多肽本身。在另一个实施方案中，靶多肽的存在和/或量与试剂例如涉及靶多肽的翻译后修饰的酶的存在和/或量和/或活性有关。第一结合剂和结合位点之间的相互作用使切割诱导部分紧密接近可切割部分，其可与多肽相关，并且仅在接近切割诱导部分时易于切割。以这种方式，可以释放每个多肽的电泳标签。分离释放的电泳标签，并且基于相应的电泳标签确定靶多肽的存在和/或量。

32. 发明授权

US06649351B2 Methods for detecting a plurality of analytes by mass spectrometry 有权
公开(公告)号：US06649351B2
公开(公告)日：2003-11-18
申请号：US10008593
申请日：2001-11-09
申请人： Tracy J. Matray , Vincent S. Hernandez , Ahmed Chenna , Herbert Hooper , Sharat Singh
发明人： Tracy J. Matray , Vincent S. Hernandez , Ahmed Chenna , Herbert Hooper , Sharat Singh
IPC分类号： C12Q168
CPC分类号： C07H19/06 , C07H19/10 , C07H21/00 , C12Q1/6816 , C40B20/08 , C40B40/08 , C40B50/16 , C40B70/00 , G01N33/561 , C12Q2521/319 , C12Q2563/131 , C12Q2565/627
摘要： The invention provides a method for detecting a target analyte, by: (a) contacting one or more target analytes with a set of first and second binding reagents under conditions sufficient for binding of a target analyte with the first and second binding reagents, each of the first binding reagents containing a cleavage-inducing moiety and a target binding moiety, each of the second binding reagents containing a tagged probe having a mass modifier region attached to a target binding moiety by a cleavable linkage, the cleavable linkage being susceptible to cleavage when in proximity to an activated cleavage-inducing moiety; (b) activating the cleavage-inducing moiety to release a tag reporter, and (c) detecting a mass of the tag reporter, the mass uniquely corresponding to a known target analyte.

33. 发明申请

US20190077814A1 BORON-CONTAINING SMALL MOLECULES 审中-公开
公开(公告)号：US20190077814A1
公开(公告)日：2019-03-14
申请号：US16081902
申请日：2017-02-27
申请人： Yong-Kang ZHANG , Perry MATTHEW , Xianfeng LI , Christopher S. LUNDE , Robert JACOBS , Vincent HERNANDEZ , Yi Palo XIA , Jacob PLATTNER , Kathy Jingyuan CAO , Bill and Melinda Gates Foundation , BILL AND MELINDA GATES FOUNDATION
发明人： Xianfeng Li , Christopher S. Lunde , Robert T. Jacobs , Vincent S. Hernandez , Yi Xia , Jacob J. Plattner , Kathy Jingyuan Cao , Yong-Kang Zhang , Matthew Perry
IPC分类号： C07F5/02 , A61P31/04
摘要： Compounds, pharmaceutical formulations, and methods of treating bacterial infections are disclosed.

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