专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

31. 发明授权

US09348127B2 Methods and apparatuses for structured illumination microscopy 有权
标题翻译：结构照明显微镜的方法和装置
公开(公告)号：US09348127B2
公开(公告)日：2016-05-24
申请号：US13121466
申请日：2009-09-22
申请人： Michael Kempe , Gerhard Krampert , Ingo Kleppe , Ralf Wolleschensky
发明人： Michael Kempe , Gerhard Krampert , Ingo Kleppe , Ralf Wolleschensky
IPC分类号： G06K9/40 , G02B21/00 , G01N21/64 , G02B21/36 , G02B27/58
CPC分类号： G02B21/0032 , G01N21/6458 , G02B21/0072 , G02B21/0076 , G02B21/008 , G02B21/367 , G02B27/58
摘要： In structured illumination microscopy, the multiple recording of images with different phase positions of the structuring requires a high stability in the optical arrangement and sample throughout the entire measuring process. Also, the structuring must be projected into the sample in a highly homogeneous manner. The current invention optimizes recording of individual images in order to achieve the best possible resolution in the result image even in problematic samples. An optimization of this kind can be carried out in different ways, for example, by determining an optimal adjustment for at least one illumination parameter or recording parameter or by pulsed illumination such that an excitation from a triplet state of the fluorescent dye to a higher triplet state is reduced, or by illuminating the sample with depletion light for depopulating a triplet state of the fluorescent dye, which reduces bleaching.
摘要翻译：在结构化照明显微镜中，具有结构化不同相位位置的图像的多次记录在整个测量过程中需要光学布置和样品中的高稳定性。此外，结构化必须以高度均匀的方式投射到样品中。本发明优化各个图像的记录，以便即使在有问题的样本中也能在结果图像中实现最佳分辨率。可以以不同的方式进行这种优化，例如通过确定至少一个照明参数或记录参数的最佳调整或通过脉冲照明使得从荧光染料的三重态到更高三重态的激发或通过用耗尽光照射样品来减少荧光染料的三线态，从而减少漂白。

32. 发明授权

US08537461B2 Method and configuration for the optical detection of an illuminated specimen 有权
标题翻译：照明样本的光学检测方法和结构
公开(公告)号：US08537461B2
公开(公告)日：2013-09-17
申请号：US12323054
申请日：2008-11-25
申请人： Michael Schwertner , Ralf Wolleschensky , Michael Kempe
发明人： Michael Schwertner , Ralf Wolleschensky , Michael Kempe
IPC分类号： G02B21/06
CPC分类号： G02B21/14 , G02B21/0024 , G02B21/06
摘要： A method for the optical detection of an illuminated specimen, wherein the illuminating light impinges in a spatially structured manner in at least one plane on the specimen and several images of the specimen are acquired by a detector in different positions of the structure on the specimen. An optical sectional image and/or an image with enhanced resolution is then calculated. The method includes generating a diffraction pattern in the direction of the specimen in or near the pupil of the objective lens or in a plane conjugate to the pupil. A structured phase plate with regions of varying phase delays is dedicated to the diffraction pattern in or near the pupil of the objective lens or in a plane conjugate to said pupil, and different phase angles of the illuminating light are set.
摘要翻译：一种用于照射样本的光学检测的方法，其中所述照明光以空间结构的方式照射在所述样本上的至少一个平面中，并且所述样本的多个图像由所述样本上的所述结构的不同位置的检测器获取。然后计算出具有增强的分辨率的光学截面图像和/或图像。该方法包括在物镜的瞳孔中或其附近或在与瞳孔共轭的平面中的样本的方向上产生衍射图案。具有不同相位延迟的区域的结构化相位板专用于物镜瞳孔中或附近的衍射图案或与所述瞳孔共轭的平面，并且设置照明光的不同相位角。

33. 发明授权

US08207510B2 Method and arrangement for collimated microscopic imaging 有权
标题翻译：准直显微镜成像的方法和布置
公开(公告)号：US08207510B2
公开(公告)日：2012-06-26
申请号：US13042137
申请日：2011-03-07
申请人： Michael Kempe , Gerhard Krampert , Matthias Wald , Ralf Wolleschensky
发明人： Michael Kempe , Gerhard Krampert , Matthias Wald , Ralf Wolleschensky
IPC分类号： G01J1/58
CPC分类号： G02B21/16 , G01N21/6458 , G02B21/0032 , G02B21/0072 , G02B21/0076
摘要： A method and arrangement for collimated microscopic imaging, including a first illumination of a sample in at least one region for exciting fluorescence, and a spatially resolving detection of the sample light by detector elements, the detection being associated with the region, wherein by means of a second illumination a sub-division of the region into separate fluorescent partial regions occurs, which are associated with the detector elements. The separation of the partial regions is carried out by the spatial separation of the fluorescent regions by means of intermediate regions having reduced fluorescence or no fluorescence, and/or by means of different spectral properties of the fluorescence from the partial regions.
摘要翻译：一种用于准直显微镜成像的方法和装置，包括用于激发荧光的至少一个区域中的样品的第一照明，以及由检测器元件对样品光进行空间分辨检测，所述检测与所述区域相关联，其中通过发生与检测器元件相关联的区域的分割成分离的荧光部分区域的第二照明。部分区域的分离是通过荧光区域通过具有减少的荧光或无荧光的中间区域和/或通过来自部分区域的荧光的不同光谱性质的空间分离来进行的。

34. 发明申请

US20110238046A1 DEVICE AND METHOD FOR CONTROLLING A LASER THERAPY OF THE EYE 有权
标题翻译：用于控制眼睛激光治疗的装置和方法
公开(公告)号：US20110238046A1
公开(公告)日：2011-09-29
申请号：US13070217
申请日：2011-03-23
申请人： Manfred Dick , Martin Hacker , Michael Kempe
发明人： Manfred Dick , Martin Hacker , Michael Kempe
IPC分类号： A61B18/20
CPC分类号： A61B3/102 , A61B5/0093 , A61B5/15 , A61F9/00821 , A61F2009/00851 , A61F2009/00863 , G01B9/02091
摘要： A device for controlling a laser therapy of the eye, including an evaluation unit that determines an intensity of a transient temperature effect by analysis of interferometric signals obtained from the eye and a control unit that controls the laser therapy, which is based on said transient temperature effect, the control unit being connected with said evaluation unit. A method for controlling a laser therapy of the eye, includes determination of the intensity of a transient temperature effect that is utilized for the control of the laser therapy, based on the effect, by analysis of interferometric signals.
摘要翻译：一种用于控制眼睛的激光治疗的装置，包括通过分析从眼睛获得的干涉信号和控制激光治疗的控制单元来确定瞬时温度效应的强度的评估单元，其基于所述瞬态温度所述控制单元与所述评估单元连接。用于控制眼睛的激光治疗的方法包括通过分析干涉信号来确定用于激光治疗的控制的瞬时温度效应的强度。

35. 发明申请

US20110226965A1 INCREASED RESOLUTION MICROSCOPY 有权
标题翻译：增加分辨率显微镜
公开(公告)号：US20110226965A1
公开(公告)日：2011-09-22
申请号：US13131801
申请日：2009-11-14
申请人： Ralf Wolleschensky , Ingo Kleppe , Gerhard Krampert , Michael Kempe
发明人： Ralf Wolleschensky , Ingo Kleppe , Gerhard Krampert , Michael Kempe
IPC分类号： G01N21/64
CPC分类号： G02B21/16 , G01N21/6458 , G02B21/361 , G02B21/367 , G02B27/58
摘要： Method for spatially high-resolution luminescence microscopy in which label molecules in a sample are activated to emit luminescence radiation comprising activating only a subset of the label molecules in the sample, wherein activated label molecules have a distance to the closest activated molecules that is greater or equal to a length which results from a predetermined optical resolution, detecting the luminescence radiation, generating a frame from the luminescence radiation, identifying the geometric locations of the label molecules with a spatial resolution increased above the predetermined optical resolution, repeating the steps and forming a combined image, and controlling the acquisition of the several frames by evaluating at least one of the frames or a group of the frames and modifying at least one variable for subsequent repetitions of the steps of generating frames for combining into an image.
摘要翻译：用于空间高分辨率发光显微镜的方法，其中样品中的标记分子被激活以发射发光，包括仅激活样品中标记分子的一个子集，其中激活的标记分子与最接近的激活分子的距离更大或等于由预定的光学分辨率产生的长度，检测发光辐射，从发光辐射产生帧，以高于预定的光学分辨率的空间分辨率识别标签分子的几何位置，重复步骤并形成并且通过评估所述帧或一组帧中的至少一个并且修改至少一个变量来控制所述几帧的获取，以便后续重复生成用于组合成图像的帧的步骤。

36. 发明授权

US07709809B2 Microscope with higher resolution and method for increasing same 有权
标题翻译：显微镜具有更高的分辨率和增加方法
公开(公告)号：US07709809B2
公开(公告)日：2010-05-04
申请号：US11806459
申请日：2007-05-31
申请人： Michael Kempe
发明人： Michael Kempe
IPC分类号： F21V9/16
CPC分类号： G02B21/0056 , G02B21/0076
摘要： Microscope with higher resolution with partial spatial superposition in the illumination by an excitation beam and a de-excitation beam and/or a switching beam in a fluorescing sample, whereby the light from the sample is deflected, whereby, in the excitation beam and/or in the de-excitation and/or the switching beam, at least one combination of devices exercising circular and radial influence on the spatial phase is provided.
摘要翻译：具有较高分辨率的显微镜，其中通过荧光样品中的激发光束和去激发光束和/或切换光束在照明中具有部分空间叠加，由此来自样品的光被偏转，由此在激发光束和/或在去激励和/或切换光束中，提供对空间相位进行圆周和径向影响的装置的至少一个组合。

37. 发明授权

US07485875B2 Resolution-enhanced luminescence microscopy 有权
标题翻译：分辨率增强发光显微镜
公开(公告)号：US07485875B2
公开(公告)日：2009-02-03
申请号：US11491781
申请日：2006-07-24
申请人： Ralf Wolleschensky , Michael Kempe
发明人： Ralf Wolleschensky , Michael Kempe
IPC分类号： G21H3/02 , G01N21/64
CPC分类号： G01N21/6458 , G02B21/0072 , G02B21/0076 , G02B21/16
摘要： A resolution-enhanced luminescence microscopy method, wherein a sample is excited so as to luminesce, and thus to emit a given luminescence radiation, by irradiation of exciting radiation and an image of the luminescent sample is obtained, wherein the luminescent sample is transferable from a first state of luminescence, in which first state the sample's excitability for emission of the given luminescence radiation increases up to a maximum value as the exciting radiation power increases, into a second state of luminescence, in which second state the sample has reduced excitability for emission of the given luminescence radiation relative to the first state, wherein the maximum value is assigned to a threshold value of exciting radiation power and the sample is transferable into the second state by irradiation of exciting radiation power above the threshold value, the sample being brought into the first state in partial areas and being brought into the second state in adjacent partial areas by irradiating exciting radiation with an exciting radiation distribution having at least one spatial power maximum above the threshold value, the image of the luminescent sample comprising sample areas being in the first state and sample areas being in the second state, sample areas being in the first state contributing predominantly to the image and the image thus having an enhanced spatial resolution with respect to the exciting radiation distribution.
摘要翻译：一种分辨率增强型发光显微镜方法，其中通过照射激发辐射激发样品以发光，从而发射给定的发光辐射，并且获得发光样品的图像，其中发光样品可从发光的第一状态，其中第一状态是给定发光辐射的发射的兴奋性随着激发辐射功率的增加而增加到最大值，变成第二种状态，其中第二种状态是样品具有降低的发射兴奋性的给定发光辐射相对于第一状态，其中最大值被分配给激发辐射功率的阈值，并且样品可通过超过阈值的激发辐射功率的照射而转移到第二状态，将样品置入部分地区的第一个状态，并在相邻的部分地区进入第二个状态通过用具有高于阈值的至少一个空间功率最大值的激发辐射分布来照射激发辐射的区域，所述发光样品的图像包括样品区域处于第一状态，样品区域处于第二状态，样品区域在主要对图像和图像贡献的第一状态因此相对于激发辐射分布具有增强的空间分辨率。

38. 发明申请

US20080192128A1 Device and Method for Generating an Image of an Object 审中-公开
标题翻译：用于生成对象图像的设备和方法
公开(公告)号：US20080192128A1
公开(公告)日：2008-08-14
申请号：US12056042
申请日：2008-03-26
申请人： Michael Kempe , Ralf Wolleschensky
发明人： Michael Kempe , Ralf Wolleschensky
IPC分类号： H04N5/235
CPC分类号： G02B21/0032 , G02B21/006
摘要： A device for generating an image of an object is provided, comprising an illumination module, by means of which the object can be illuminated with a pattern whose phase is altered temporally, a recording module, by means of which a plurality of recordings of the object are carried out during the phase change of the pattern, and a processing module, which generates the image from the recordings, wherein the illumination module moves a light beam over the object and modulates its intensity synchronously with the movement such that the beam generates the pattern in scanning fashion.
摘要翻译：提供了一种用于产生物体的图像的装置，包括照明模块，利用该照明模块可以利用其相位在时间上改变的图案来照亮物体;记录模块，借助于该物体的多个记录在图案的相变期间进行，以及处理模块，其从记录产生图像，其中照明模块将光束移动到物体上并且与移动同步地调制其强度，使得光束产生图案在扫描时尚。

39. 发明申请

US20060152791A1 Method and arrangement for optical examination or processing of a sample 有权
公开(公告)号：US20060152791A1
公开(公告)日：2006-07-13
申请号：US10539491
申请日：2003-11-29
申请人： Ralf Wolleschensky , Michael Kempe
发明人： Ralf Wolleschensky , Michael Kempe
IPC分类号： G02F1/00
CPC分类号： G02B21/0076 , G02B21/0032
摘要： A method and an arrangement for the optical examination and/or processing of a sample comprise an element for generating an illumination light, an element arranged downstream of the latter for spectral splitting of the illumination light for generating spatially separated spectral components, an element for parallelizing the split illumination light, an element for focusing the illumination light on or in the sample, wherein the spectral components are superposed, and an element for detecting the sample light, advantageously comprising an element for generating a short-pulse illumination light, an element arranged downstream of the latter for spectral splitting of the illumination light for generating spatially separated spectral components with pulse lengths that are greater than the pulse length of the illumination light, wherein these spectral components are combined again in the sample.

40. 发明授权

US06947127B2 Arrangement for the optical capture of excited and/or back scattered light beam in a sample 有权
标题翻译：用于光学捕获样品中激发和/或反向散射光束的布置
公开(公告)号：US06947127B2
公开(公告)日：2005-09-20
申请号：US10313649
申请日：2002-12-06
申请人： Ralf Wolleschensky , Michael Kempe
发明人： Ralf Wolleschensky , Michael Kempe
IPC分类号： G02B21/00 , G02B21/06 , G02B21/16 , G01N21/00 , G01J1/42
CPC分类号： G02B21/0076 , G02B21/06 , G02B21/16
摘要： An arrangement for the optical detection of light radiation which is excited and/or backscattered in a specimen in a microscope, wherein the specimen illumination is focused in and/or in the vicinity of pupil plane of the beam path between the specimen plane and detection plane and apparatus is provided in this plane for spatially separating the illumination light from the detection light, wherein fluorescent light and/or luminescent light and/or phosphorescent light and/or diffusely scattered illumination light coming from the specimen is detected, and the apparatus for spatial separation comprises at least a reflecting first portion and at least a transmitting second portion, wherein the reflecting portion serves to couple in the illumination light and the transmitting portion serves to pass the detection light in the detection direction or the transmitting portion serves to couple in the illumination light and the reflecting portion serves to couple out the detection light.
摘要翻译：一种用于在显微镜中在样本中被激发和/或反向散射的光辐射的光学检测装置，其中所述检体照射被聚焦在所述检测平面和检测平面之间的光束路径的光瞳面附近和/或附近并且在该平面中设置用于将来自检测光的照明光空间分离的装置，其中检测来自样本的荧光和/或发光和/或磷光和/或漫散射的照明光，并且用于空间的装置分离至少包括反射第一部分和至少透射第二部分，其中反射部分用于在照明光中耦合，并且透射部分用于使检测光在检测方向上通过，或者透射部分用于在照明光和反射部分用于耦合出检测光。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式