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31. 发明授权

US08329428B2 Enzyme that catalyzes a peptide-forming reaction from a carboxy component and an amine component, microbe producing the same, and a method of producing a dipeptide using the enzyme or microbe 有权
标题翻译：从羧基组分和胺组分催化肽形成反应的酶，产生其的微生物以及使用酶或微生物产生二肽的方法
公开(公告)号：US08329428B2
公开(公告)日：2012-12-11
申请号：US13211834
申请日：2011-08-17
申请人： Seiichi Hara , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
发明人： Seiichi Hara , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
IPC分类号： C12P21/06 , C12N9/00 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/00 , C12N9/93 , C12P21/00 , C12P21/02
摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
摘要翻译：公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

32. 发明申请

US20120295304A1 METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-BETA-ESTER AND METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-ALPHA-METHYL ESTER 有权
公开(公告)号：US20120295304A1
公开(公告)日：2012-11-22
申请号：US13528126
申请日：2012-06-20
申请人： Kenzo YOKOZEKI , Ayako Ohno , Seiichi Hara , Isao Abe
发明人： Kenzo YOKOZEKI , Ayako Ohno , Seiichi Hara , Isao Abe
IPC分类号： C12P21/02
CPC分类号： C07K5/0613 , A23L27/32 , C07K5/06113 , C12P21/02 , C12P21/06
摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.

33. 发明授权

US08084227B2 Method for producing dipeptides 失效
标题翻译：二肽生产方法
公开(公告)号：US08084227B2
公开(公告)日：2011-12-27
申请号：US11330076
申请日：2006-01-12
申请人： Kenzo Yokozeki , Isao Abe , Seiichi Hara
发明人： Kenzo Yokozeki , Isao Abe , Seiichi Hara
IPC分类号： C12P21/06 , C12P1/00 , C12N9/00 , C12N9/10
CPC分类号： C12P21/02
摘要： The present invention provides a method for producing a dipeptide from starting materials that are available at low costs through a route industrially advantageous and simple. Dipeptides are produced from amino acid esters and amino acids by using a culture of a microbe having an ability to produce a dipeptide from an amino acid ester and an amino acid, microbial cells separated from the culture, or treated microbial cell product.
摘要翻译：本发明提供了一种通过工业上有利且简单的途径以低成本获得的起始材料制备二肽的方法。通过使用具有从氨基酸酯和氨基酸产生二肽的能力的微生物培养物，从培养物分离的微生物细胞或经处理的微生物细胞产物，由氨基酸酯和氨基酸产生二肽。

34. 发明申请

US20100267082A1 NOVEL PEPTIDE-FORMING ENZYME GENE 有权
标题翻译：新型肽形成酶基因
公开(公告)号：US20100267082A1
公开(公告)日：2010-10-21
申请号：US12729656
申请日：2010-03-23
申请人： Seiichi Hara , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
发明人： Seiichi Hara , Kenzo Yokozeki , Isao Abe , Naoto Tonouchi , Yasuko Jojima
IPC分类号： C12P21/06 , C12N9/00 , C12N1/21 , C07H21/04
CPC分类号： C12N9/00 , C12N9/93 , C12P21/00 , C12P21/02
摘要： DNA and recombinant DNA that encode a peptide-forming enzyme, a method for producing a peptide-forming enzyme, and a method for producing a dipeptide are disclosed. A method for producing a dipeptide includes producing a dipeptide from a carboxy component and an amine component by using a culture of a microbe belonging to the genus Sphingobacterium and having the ability to form the dipeptide from the carboxy component and the amine component, a microbial cell separated from the culture, treated microbial cell product of the microbe or a peptide-forming enzyme derived from the microbe.
摘要翻译：公开了编码肽形成酶的DNA和重组DNA，产生肽形成酶的方法及其制备方法。制备二肽的方法包括通过使用属于鞘氨醇杆菌属的微生物的培养物从羧基组分和胺组分制备二肽并具有从羧基组分和胺组分形成二肽的能力，微生物细胞与培养物分离，处理微生物的微生物细胞产物或源自微生物的肽形成酶。

35. 发明授权

US07749742B2 Method for producing tripeptides and/or peptides longer than tripeptides 失效
标题翻译：生产长于三肽的三肽和/或肽的方法
公开(公告)号：US07749742B2
公开(公告)日：2010-07-06
申请号：US11940555
申请日：2007-11-15
申请人： Kenzo Yokozeki , Sonoko Suzuki , Seiichi Hara , Isao Abe
发明人： Kenzo Yokozeki , Sonoko Suzuki , Seiichi Hara , Isao Abe
IPC分类号： C12N9/10 , C12N1/20 , C12N15/00 , C12P21/06 , C12P13/04 , A61K38/00 , C07H21/04
CPC分类号： C07K5/06 , C12N9/16 , C12N9/52 , C12N9/80 , C12P21/02
摘要： A method is disclosed that allows the production of peptides having three or more amino acid residues easily, inexpensively and at high yield without going through a complex synthesis method. A novel enzyme that efficiently produces a peptide from bacteria belonging to the genus Empedobacter or the genus Sphingobacterium is provided. The enzyme acts on a carboxy component and an amine component to form peptides having three or more amino acid residues by acting on a carboxy component and an amine component.
摘要翻译：公开了一种能够容易地，低成本地以高产率制备具有三个或更多个氨基酸残基的肽而不经过复合合成方法的方法。提供了一种新颖的酶，其有效地从属于Empedobacter属或Sphingobacterium属的细菌产生肽。该酶作用于羧基组分和胺组分以通过作用于羧基组分和胺组分形成具有三个或更多个氨基酸残基的肽。

36. 发明授权

US07745172B2 Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester 有权
标题翻译：制备α-L-天冬氨酰基-L-苯丙氨酸的方法和制备α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的方法
公开(公告)号：US07745172B2
公开(公告)日：2010-06-29
申请号：US11970203
申请日：2008-01-07
申请人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
发明人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
IPC分类号： C12P21/06
CPC分类号： C07K5/0613 , A23L27/32 , C07K5/06113 , C12P21/02 , C12P21/06
摘要： A method for producing an α-L-aspartyl-L-phenylalanine-β-ester (also named α-L-(β-o-substituted aspartyl)-L-phenylalanine), which is an intermediate of an α-L-aspartyl-L-phenylalanine-α-methyl ester (also named α-L-aspartyl-L-phenylalanine methyl ester; product name: aspartame), easily, at high yield and inexpensively without going through a complex synthesis method is provided. Further, an easy, inexpensive and high-yield production method for an α-L-aspartyl-L-phenylalanine-α-methyl ester is provided. α-L-aspartyl-L-phenylalanine-α-methyl ester is produced from a L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to catalyze a reaction in which L-phenylalanine performs no nucleophilic attack on a β-ester site of L-aspartic acid-α,β-diester but performs a nucleophilic attack on an α-ester site thereof.
摘要翻译：制备α-L-天冬氨酰基-L-苯丙氨酸 - 酯（也称为α-L- - （取代的天冬氨酰基）-L-苯丙氨酸）的方法，其为α-L 提供了天竺葵酰-L-苯丙氨酸-α-甲酯（也称为α-L-天冬氨酰基-L-苯丙氨酸甲酯;产品名称：阿斯巴甜），容易地以高产率和低成本而不经过复合合成方法。此外，提供了一种用于α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的容易且廉价且高产率的制备方法。使用具有催化反应能力的酶或酶的物质由L-天冬氨酸-α，β-二酯和L-苯丙氨酸制备α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯其中L-苯丙氨酸对L-天冬氨酸-α，bgr-siester的“酯”位点不发生亲核攻击，但对其α-酯位点进行亲核攻击。

37. 发明授权

US07115389B2 Method for producing dipeptide 失效
标题翻译：二肽生产方法
公开(公告)号：US07115389B2
公开(公告)日：2006-10-03
申请号：US11008203
申请日：2004-12-10
申请人： Seiichi Hara , Kenzo Yokozeki
发明人： Seiichi Hara , Kenzo Yokozeki
IPC分类号： C12P21/06
CPC分类号： C07K5/06026 , C12P21/02 , Y10S435/823 , Y10S435/849
摘要： A method for producing a dipeptide including the step of exposing a carboxy component and an amine component to an enzyme having an activity to hydrolyze amino acid ester. Such a hydrolase has been found among enzymes in bacteria. The method is useful for producing a peptide simply and inexpensively with a high yield without taking complicate synthetic methods.
摘要翻译：一种制备二肽的方法，包括将羧基组分和胺组分暴露于具有水解氨基酸酯活性的酶的步骤。这种水解酶已经在细菌的酶中发现。该方法可用于在不采取复杂的合成方法的情况下以高产率简单且廉价地生产肽。

38. 发明申请

US20050221668A1 Connector capable of being firmly fixed to an object and a fixing member used in the connector 有权
标题翻译：能够牢固地固定在物体上的连接器和在连接器中使用的固定部件
公开(公告)号：US20050221668A1
公开(公告)日：2005-10-06
申请号：US11092536
申请日：2005-03-29
申请人： Seiichi Hara , Tadashi Ishiwa , Yasufumi Hayashi
发明人： Seiichi Hara , Tadashi Ishiwa , Yasufumi Hayashi
IPC分类号： H01R13/73 , H01R12/14 , H01R13/60
CPC分类号： H01R12/7029
摘要： In a connector including an insulator and a contact held by the insulator, a fixing member is coupled to the insulator for fixing the insulator to an object. The fixing member includes a main body received in a receiving portion formed to the insulator. A leg mechanism extends from the main body for being fixed to the object. In addition, a first engaging mechanism extends from the main body and is engaged with the receiving portion in a first direction. On the other hand, the second engaging mechanism extends from the main body and is engaged with the insulator in a second direction opposite to the first direction.
摘要翻译：在包括绝缘体和由绝缘体保持的接触件的连接器中，固定构件联接到绝缘体，以将绝缘体固定到物体上。固定构件包括容纳在形成于绝缘体的容纳部中的主体。腿部机构从主体延伸以固定到物体。此外，第一接合机构从主体延伸并且沿着第一方向与接收部分接合。另一方面，第二接合机构从主体延伸并与第一方向相反的第二方向与绝缘体接合。

39. 发明授权

US08361748B2 Method for producing α-L-aspartyl-L-phenylalanine-β-ester and method for producing α-L-aspartyl-L-phenylalanine-α-methyl ester 有权
标题翻译：制备α-L-天冬氨酰基-L-苯丙氨酸的方法和制备α-L-天冬氨酰基-L-苯丙氨酸-α-甲酯的方法
公开(公告)号：US08361748B2
公开(公告)日：2013-01-29
申请号：US13528162
申请日：2012-06-20
申请人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
发明人： Kenzo Yokozeki , Ayako Ohno , Seiichi Hara , Isao Abe
IPC分类号： C12P21/06
CPC分类号： C07K5/0613 , A23L27/32 , C07K5/06113 , C12P21/02 , C12P21/06
摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.
摘要翻译：通过从L-天冬氨酸-α，二酯和L形成α-L-天冬氨酰基-L-苯丙氨酸 - 乙酸酯制备α-L-天冬氨酰基-L-苯丙氨酸 - 酯的方法 - 苯丙氨酸，其使用具有通过肽键将L-苯丙氨酸选择性地连接到L-天冬氨酸-α，α-二酯的α-酯位点的酶或酶的物质。

40. 发明申请

US20120301918A1 METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-BETA-ESTER AND METHOD FOR PRODUCING ALPHA-L-ASPARTYL-L-PHENYLALANINE-ALPHA-METHYL ESTER 有权
公开(公告)号：US20120301918A1
公开(公告)日：2012-11-29
申请号：US13528162
申请日：2012-06-20
申请人： Kenzo YOKOZEKI , Ayako Ohno , Seiichi Hara , Isao Abe
发明人： Kenzo YOKOZEKI , Ayako Ohno , Seiichi Hara , Isao Abe
IPC分类号： C12P21/02
CPC分类号： C07K5/0613 , A23L27/32 , C07K5/06113 , C12P21/02 , C12P21/06
摘要： A method of producing an α-L-aspartyl-L-phenylalanine-β-ester by forming the α-L-aspartyl-L-phenylalanine-β-ester from L-aspartic acid-α,β-diester and L-phenylalanine using an enzyme or enzyme-containing substance that has an ability to selectively link L-phenylalanine to an α-ester site of the L-aspartic acid-α,β-diester through a peptide bond.

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