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    • 31. 发明授权
    • Process for preparing polyhydroxyalkanoate employing maoC gene
    • 使用maoC基因制备聚羟基链烷酸酯的方法
    • US07183087B2
    • 2007-02-27
    • US10662358
    • 2003-09-16
    • Sang Yup LeeSi Jae Park
    • Sang Yup LeeSi Jae Park
    • C12P7/62C12N1/20C12N9/88C12N15/00C07H21/04
    • C12N9/93C08G63/06C12N9/88C12P7/625C12Y402/01017
    • The present invention relates to a method for producing middle-chain-length polyhydroxyalkanoate (MCL-PHA) using a maoC gene. The producing method of MCL-PHA according to the present invention comprises the steps of transforming a microorganism with the maoC gene to give a transformant, the microorganism being deleted of a fadB gene and containing a PHA synthase gene; culturing the transformant in medium containing a C6-10 carbon source; and obtaining PHA consisting of monomers with 6–10 carbon atoms. When the maoC gene whose function has not yet been established is used according to the present invention, high quality PHA with a higher number of carbon atoms than the prior PHA can be produced at a higher efficiency.
    • 本发明涉及使用maoC基因生产中链长度聚羟基链烷酸酯(MCL-PHA)的方法。 根据本发明的MCL-PHA的制备方法包括以下步骤:用maoC基因转化微生物以产生转化体,所述微生物缺失fadB基因并含有PHA合酶基因; 在含有C 6-10碳源的培养基中培养转化体; 并获得由具有6-10个碳原子的单体组成的PHA。 当根据本发明使用尚未建立功能的maoC基因时,可以以更高的效率生产具有比先前的PHA高的碳原子数的高质量PHA。
    • 35. 发明授权
    • Method of producing fatty acid alkyl ester using microorganisms having ability to produce oil
    • 使用能够生产油的微生物生产脂肪酸烷基酯的方法
    • US09322004B2
    • 2016-04-26
    • US13384576
    • 2010-07-19
    • Sang Yup LeeYong Jun Choi
    • Sang Yup LeeYong Jun Choi
    • C12P7/64C12N9/20
    • C12N9/20C12P7/649C12Y301/01003Y02E50/13Y02P20/52
    • The present invention relates to a method of producing a fatty acid alkyl ester using microorganisms having the ability to produce oil, and more particularly to a method of producing a fatty acid alkyl ester, the method comprising culturing microorganisms having the ability to produce oil, thus accumulating a large amount of oil in the microorganisms, inducing the autolysis of the produced oil in the microorganisms to produce a free fatty acid, and converting the free fatty acid into an alkyl ester. According to the method of the present invention, oil accumulated in microorganisms, such as triacylglycerol that is typical oil produced by microorganisms, can be converted into a fatty acid alkyl ester with high efficiency using a metabolic engineering approach. Thus, the method of the present invention is useful for the industrial production of a fatty acid alkyl ester which has been recently found to be effective as biodiesel.
    • 本发明涉及使用具有生产油的能力的微生物生产脂肪酸烷基酯的方法,更具体地说,涉及生产脂肪酸烷基酯的方法,该方法包括培养具有生产油的微生物,从而 在微生物中积聚大量的油,引起微生物中产生的油的自溶以产生游离脂肪酸,并将游离脂肪酸转化为烷基酯。 根据本发明的方法,可以使用代谢工程方法将积累在微生物中的微生物油(例如由微生物产生的典型油的三酰基甘油)的油转化为高效率的脂肪酸烷基酯。 因此,本发明的方法可用于最近被发现作为生物柴油有效的脂肪酸烷基酯的工业生产。
    • 38. 发明授权
    • Method for preparing metal nanoparticle using metal binding protein
    • 使用金属结合蛋白制备金属纳米粒子的方法
    • US08476055B2
    • 2013-07-02
    • US12301581
    • 2007-04-17
    • Sang Yup LeeTae Jung Park
    • Sang Yup LeeTae Jung Park
    • C12N1/20C07H21/04
    • C07K14/825B82Y5/00B82Y15/00C12N9/104C12P3/00G01N33/531G01N33/588Y10T428/2982
    • The present invention relates to a method of preparing heavy metal nanoparticles using a heavy metal-binding protein. More specifically, relates to a method for preparing heavy metal structures, comprising the steps of: culturing a microorganism transformed with a gene encoding a heavy metal-binding protein, in a heavy metal ion-containing medium, to produce heavy metal structures in the microorganism; and collecting the produced heavy metal structures, as well as nanoparticles of heavy metal structures prepared according to said method. Unlike prior methods of preparing quantum dots by physically binding metal materials, the quantum dots disclosed herein can be efficiently produced by expressing the heavy metal-binding protein in cells. In addition, the quantum dots are useful because they can solve an optical stability problem that is the shortcoming of organic fluorophores.
    • 本发明涉及使用重金属结合蛋白制备重金属纳米粒子的方法。 更具体地,涉及一种制备重金属结构体的方法,包括以下步骤:在含重金属离子的培养基中培养用重金属结合蛋白的基因转化的微生物,以在微生物中产生重金属结构 ; 并收集所生产的重金属结构物,以及根据所述方法制备的重金属结构纳米颗粒。 与通过物理结合金属材料制备量子点的现有方法不同,本文公开的量子点可以通过在细胞中表达重金属结合蛋白而有效地产生。 此外,量子点是有用的,因为它们可以解决作为有机荧光团的缺点的光学稳定性问题。