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    • 32. 发明授权
    • Identification of salmonella
    • 鉴定沙门氏菌
    • US06368817B1
    • 2002-04-09
    • US09355066
    • 1999-07-08
    • John David PerryMichael Ford
    • John David PerryMichael Ford
    • C12Q102
    • C12Q1/045C12Q1/04C12Q2334/20C12Q2334/52G01N2333/255G01N2333/924
    • A new culture medium for identifying the presence of Salmonella inenterobacteria samples, especially faeces, contains two chromogenic enzyme substrates, one of which is a substrate for &agr;-D-galactosidase, for which Salmonella is positive. The other substrate is one for which Salmonella is negative such as &bgr;-D-galactosidase. The substrates are incorporated into an agar medium. Positive and negative results are found to be readily observable where one of the substrates is an esculetin, preferably a cyclohexenoesculetin compound in the presence of ferric ions, which produces a black color, and the other substrate is an indoxyl compound, for instance, a 5-bromo-4-chloro-3-indolyl compound which produces a green colored enzymic reaction product.
    • 用于鉴定肠杆菌样品,特别是粪便中沙门氏菌存在的新培养基含有两种显色酶底物,其中一种是用于α-D-半乳糖苷酶的底物,其中沙门氏菌是阳性的。 其他底物是沙门氏菌为阴性的底物,如β-D-半乳糖苷酶。 将底物并入琼脂培养基中。 发现阳性和阴性结果可以容易地观察到,其中一种底物是七叶素,优选在三价铁离子存在下的环己二烯类锡化合物,其产生黑色,另一种底物是吲哚基化合物,例如5 - 溴-4-氯-3-吲哚基化合物,其产生绿色的酶反应产物。