专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

31. 发明授权

US5326882A Chemiluminescent 3-(substituted Adamant-2'-Ylidene) 1,2-dioxetanes 失效
标题翻译：化学发光3-（取代的金刚-2'-亚联）1,2-二氧杂环丁烷
公开(公告)号：US5326882A
公开(公告)日：1994-07-05
申请号：US849086
申请日：1992-04-30
申请人： Irena Bronstein , Brooks Edwards , Rouh-Rong Juo
发明人： Irena Bronstein , Brooks Edwards , Rouh-Rong Juo
IPC分类号： C07C43/178 , C07C43/196 , C07C43/215 , C07C69/00 , C07D321/00 , C07F9/12 , C07F9/655 , C07F9/6574 , C07H15/203 , C07H15/26 , C12Q1/34 , G01N21/76 , G01N33/58 , C07D327/06
CPC分类号： C07H15/203 , C07C43/1788 , C07C43/196 , C07C43/215 , C07C69/00 , C07D321/00 , C07F9/12 , C07F9/65512 , C07F9/65742 , C07H15/26 , C12Q1/34 , G01N33/581 , G01N33/582 , C07C2103/00 , C07C2103/74
摘要： Enzymatically cleavable chemiluminescent 1,2-dioxetane compounds capable of producing light energy when decomposed, substantially stable at room temperature before a bond by which an enzymatically cleavable labile substituent thereof is intentionally cleaved, are disclosed. These compounds can be represented by the formula: ##STR1## The corresponding dioxetanes which, instead of being substituted at the 5' or 7', or at the 5' and 7' positions, instead contain a 4' methylene group, are also disclosed, as are intermediates for all these 3-substituted adamant-2'-ylidenedioxetanes, and their use as reporter molecules in assays.
摘要翻译： PCT No.PCT / US91 / 06096 Sec。一九九二年四月三十日 102（e）日期1992年4月30日PCT 1991年8月30日PCT。可分解的化学发光的1,2-二氧杂环丁烷化合物，其在分解时能够产生光能，在室温下基本上稳定，在该键之间可通过酶促切割的不稳定取代基被公开了。这些化合物可以由下式表示：还公开了代替含有4'亚甲基的相应的二氧杂环丁烷，而不是在5'或7'或5'和7'位置被取代，，以及所有这些3-取代的金刚烷-2'-亚基二氧杂环己烷的中间体，以及它们在测定中作为报道分子的用途。

32. 发明授权

US07112413B2 Heteroaryl substituted benzothiazole dioxetanes 有权
公开(公告)号：US07112413B2
公开(公告)日：2006-09-26
申请号：US10679368
申请日：2003-10-07
申请人： Brooks Edwards , Irena Bronstein , Zhixian Wang
发明人： Brooks Edwards , Irena Bronstein , Zhixian Wang
IPC分类号： G01N33/533 , G01N33/532 , G01N21/76 , C07D277/62
CPC分类号： C07D417/04 , C07D277/62 , C07D493/04 , C07F9/65512 , C07F9/65586 , C09K9/02 , G01N33/582
摘要： Chemiluminescent heteroaryl substituted benzothiazole 1,2-dioxetane compounds capable of producing light energy when decomposed are provided. These chemiluminescent compounds are represented by the general formula: The heteroaryl substituent Y can be, for example, a pyridyl group or a benzothiazolyl group. The heteroaryl substituted benzothiazole compounds are substantially stable at room temperature. Kits including the heteroaryl substituted dioxetane compounds as well as methods for using these compounds for detecting the presence of one or more analytes in a sample are also provided.

33. 发明授权

US06660529B2 Heteroaryl substituted benzothiazole dioxetanes 有权
标题翻译：杂芳基取代的苯并噻唑二氧杂环丁烷
公开(公告)号：US06660529B2
公开(公告)日：2003-12-09
申请号：US09945652
申请日：2001-09-05
申请人： Brooks Edwards , Irena Bronstein , Zhixian Wang
发明人： Brooks Edwards , Irena Bronstein , Zhixian Wang
IPC分类号： G01N2176
CPC分类号： C07D417/04 , C07D277/62 , C07D493/04 , C07F9/65512 , C07F9/65586 , C09K9/02 , G01N33/582
摘要： Chemiluminescent heteroaryl substituted benzothiazole 1,2-dioxetane compounds capable of producing light energy when decomposed are provided. These chemiluminescent compounds are represented by the general formula: The heteroaryl substituent Y can be, for example, a pyridyl group or a benzothiazolyl group. The heteroaryl substituted benzothiazole compounds are substantially stable at room temperature. Kits including the heteroaryl substituted dioxetane compounds as well as methods for using these compounds for detecting the presence of one or more analytes in a sample are also provided.
摘要翻译：提供了能够在分解时产生光能的化学发光的杂芳基取代的苯并噻唑1,2-二氧杂环丁烷化合物。这些化学发光化合物由以下通式表示：杂芳基取代基Y可以是例如吡啶基或苯并噻唑基。杂芳基取代的苯并噻唑化合物在室温下基本稳定。还提供了包括杂芳基取代的二恶丁环化合物的试剂盒以及使用这些化合物检测样品中一种或多种分析物的存在的方法。

34. 发明授权

US5827650A Membrane for chemiluminescent blotting applications 失效
标题翻译：用于化学发光印迹应用的膜
公开(公告)号：US5827650A
公开(公告)日：1998-10-27
申请号：US590352
申请日：1996-01-23
申请人： Irena Bronstein , Brooks Edwards , John Voyta
发明人： Irena Bronstein , Brooks Edwards , John Voyta
IPC分类号： G01N21/78 , B32B27/30 , C09D125/00 , C09D125/18 , G01N33/545 , G01N33/58 , B32B27/00
CPC分类号： G01N33/582 , G01N33/545 , Y10T428/31536 , Y10T428/31743 , Y10T428/31884
摘要： A new and improved polymeric membrane for use in biological assays is provided. A blotting assay employing 1,2-dioxetanes as a source of chemiluminescent employs, as an improved membrane, a polymer comprised of at least one monomer of the formula: ##STR1## The membranes reduce background signal, improve sensitivity and reliability.
摘要翻译：提供了用于生物测定的新的和改进的聚合物膜。使用1,2-二氧杂环丁烷作为化学发光源的印迹测定使用由至少一种下式的单体组成的聚合物作为改进的膜：膜减少背景信号，提高灵敏度和可靠性。

35. 发明授权

US5593828A Membrane for chemiluminescent blotting applications 失效
标题翻译：用于化学发光印迹应用的膜
公开(公告)号：US5593828A
公开(公告)日：1997-01-14
申请号：US233850
申请日：1994-04-26
申请人： Irena Bronstein , Brooks Edwards , John Voyta
发明人： Irena Bronstein , Brooks Edwards , John Voyta
IPC分类号： G01N21/78 , B32B27/30 , C09D125/00 , C09D125/18 , G01N33/545 , G01N33/58 , B32B27/00
CPC分类号： G01N33/582 , G01N33/545 , Y10T428/31536 , Y10T428/31743 , Y10T428/31884
摘要： A new and improved polymeric membrane for use in biological assays is provided. A blotting assay employing 1,2-dioxetanes as a source of chemiluminescent employs, as an improved membrane, a polymer comprised of at least one monomer of the formula: ##STR1## The membranes reduce background signal, improve sensitivity and reliability.
摘要翻译：提供了用于生物测定的新的和改进的聚合物膜。使用1,2-二氧杂环丁烷作为化学发光源的印迹测定使用由至少一种下式的单体组成的聚合物作为改进的膜：膜减少背景信号，提高灵敏度和可靠性。

36. 发明授权

US06586196B1 Multiple enzyme assays 有权
标题翻译：多重酶测定
公开(公告)号：US06586196B1
公开(公告)日：2003-07-01
申请号：US09459982
申请日：1999-12-14
申请人： Irena Bronstein , Christopher Martin , Corinne Olesen , John Voyta , Yu-xin Yan
发明人： Irena Bronstein , Christopher Martin , Corinne Olesen , John Voyta , Yu-xin Yan
IPC分类号： C12Q142
CPC分类号： C12Q1/6897 , C12Q1/00 , C12Q1/34 , C12Q1/42 , C12Q1/66 , G01N2333/916 , G01N2333/924 , G01N2333/95
摘要： The present invention discloses multiple enzyme assays which measure the activity of at least one endogenous enzyme in a single aliquot and a method of measuring the activity of multiple enzymes in an aliquot of a cell extract, wherein at least one of the enzymes is an endogenous enzyme. In one embodiment of the invention the activity of a first enzyme is quantified by measuring the light signal produced by degradation of a first enzyme substrate by the first enzyme and the activity of the second enzyme is quantified by measuring the light signal produced by the degradation of a second substrate. In the method of the present invention, both quantifications are performed on the same aliquot of cell extract. Different embodiments of the present invention provide for the detection of more than one endogenous enzyme and for the detection of at least one reporter enzyme and at least one endogenous enzyme. The present invention also discloses kits for detecting the activity of multiple enzymes.
摘要翻译：本发明公开了测量单一等分试样中至少一种内源性酶的活性的多种酶测定法和测量细胞提取物等分试样中多种酶的活性的方法，其中至少一种酶是内源性酶。在本发明的一个实施方案中，通过测量由第一酶降解第一酶底物产生的光信号来定量第一酶的活性，并且通过测量由第一酶降解产生的光信号来量化第二酶的活性第二基板。在本发明的方法中，两种定量在相同等分的细胞提取物上进行。本发明的不同实施方案提供了多于一种内源性酶的检测和用于检测至少一种报道酶和至少一种内源性酶。本发明还公开了用于检测多种酶的活性的试剂盒。

37. 发明授权

US06243980B1 Protease inhibitor assay 失效
标题翻译：蛋白酶抑制剂测定
公开(公告)号：US06243980B1
公开(公告)日：2001-06-12
申请号：US09035820
申请日：1998-03-06
申请人： Irena Bronstein , John Voyta , Michelle Palmer , Bonnie Tillotson
发明人： Irena Bronstein , John Voyta , Michelle Palmer , Bonnie Tillotson
IPC分类号： G01N33533
CPC分类号： G01N33/582 , C12Q1/37 , G01N33/581 , G01N2333/81 , Y10S436/80 , Y10S436/805
摘要： Heterogenous and homogenous assays are provided for the detection of protease inhibitory activity in a sample or target compound, taking advantage of the chemiluminescent characteristics of 1,2-dioxetanes. In the heterogenous assay, a peptide bearing a cleavage site for the protease of interest is provided with a first member of a first ligand binding pair at one end, and a first member of a second ligand binding pair at the other end. The other member of the first ligand binding pair is attached to a surface, which binds the peptide, or protease substrate, to the surface. The peptide substrate is combined with the protease and target compound or sample. Substrate cleavage, if not inhibited, is allowed to occur, and any unbound cleaved fragments are removed. An enzyme complexed with the second member of the second ligand binding pair is added, and allowed to bind to any of the (uncleaved) first member of the second ligand binding pair remaining. Unbound complex is removed, and a 1,2-dioxetane substrate for the enzyme is added. If any peptide substrate has not been cleaved, the dioxetane will chemiluminesce, indicating inhibitory activity. In a homogenous assay, the same substrate bears at one end a fluorescent energy accepting moiety, and at the other end a 1,2-dioxetane or precursor. If the substrate is cleaved by the protease, the dioxetane and the fluorescent moiety are not in close physical relationship, and no energy transfer occurs when the dioxetane is caused to decompose. If cleavage has not occurred, indicating inhibition, when the dioxetane is caused to decompose, energy is transferred to the fluorescing entity, which releases light of a wavelength recognizably distinct from that of the dioxetane.
摘要翻译：提供异源和均一的测定法，用于检测样品或目标化合物中蛋白酶抑制活性，利用1,2-二氧杂环己烷的化学发光特性。在异源测定中，具有目的蛋白酶的切割位点的肽在一端具有第一配体结合对的第一个成员，另一端具有第二配体结合对的第一个成员。第一配体结合对的另一个成员连接到表面，其将肽或蛋白酶底物结合到表面。将肽底物与蛋白酶和目标化合物或样品组合。如果不抑制底物裂解，则可以发生底物裂解，并且去除任何未结合的切割的片段。加入与第二配体结合对的第二个成员配合的酶，并使其与残留的第二配体结合对的任何（未切割的）第一个成员结合。去除未结合的络合物，并加入酶的1,2-二氧环乙烷底物。如果任何肽底物未被切割，二氧杂环丁烷将化学发光，表明抑制活性。在同质测定中，相同的底物在一端承载荧光能量接受部分，另一端在1,2-二氧杂环丁烷或前体上。如果底物被蛋白酶切割，二氧杂环丁烷和荧光部分不是很密切的物理关系，当二氧杂环丁烷分解时，不会发生能量转移。如果没有发生裂解，表明抑制，当二氧杂环丁烷分解时，能量被转移到荧光实体，其释放与二氧杂环丁烷的波长可辨别的波长的光。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式