会员体验
专利管家(专利管理)
工作空间(专利管理)
风险监控(情报监控)
数据分析(专利分析)
侵权分析(诉讼无效)
联系我们
交流群
官方交流:
QQ群: 891211   
微信请扫码    >>>
现在联系顾问~
热词
    • 40. 发明授权
    • Cyanine dyes that stain cells and mitochondria
    • 染色细胞和线粒体的花青染料
    • US06291203B1
    • 2001-09-18
    • US09266697
    • 1998-12-03
    • Martin PootFei Mao
    • Martin PootFei Mao
    • G01N130
    • G01N1/30C09B23/02G01N2001/302
    • The present invention relates to the use of positively-charged unsymmetrical cyanine dyes, including novel unsymmetrical cyanine dyes, to stain cells and selectively stain intracellular organelles, with particular advantages in staining mitochondria. The dyes are generally weakly fluorescent in aqueous solution but highly fluorescent in cells and organelles, typically staining cells or mitochondria with a green fluorescence. The dyes stain mitochondria in both live and dead cells, and are retained in mitochondria even where the cells are treated with solvents that permeabilize cell and mitochondrial membranes. Mitochondria or cells stained according to this method are optionally stained with an additional detection reagent, such as a labeled antibody, labeled oligonucleotide, fluorogenic enzyme substrate, or other indicator for a specific cellular component or substructure, including another unsymmetrical cyanine stain of the same or different class.
    • 本发明涉及使用带正电荷的不对称花青染料,包括新颖的不对称花青染料,染色细胞和选择性染色细胞内细胞器,具有染色线粒体特别的优点。 染料通常在水溶液中弱荧光,但在细胞和细胞器中高度荧光,通常用绿色荧光染色细胞或线粒体。 染料在活细胞和死细胞中染色线粒体,并且保留在线粒体中,甚至在用细胞和线粒体膜透化的溶剂处理细胞的情况下。 线粒体或根据该方法染色的细胞任选地用另外的检测试剂如标记的抗体,标记的寡核苷酸,荧光酶底物或用于特定细胞组分或亚结构的其它指示剂进行染色,包括其它不对称花青染色 不同班