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31. 发明申请

US20100009867A1 MULTI-MODAL ION EXCHANGE CHROMATOGRAPHY RESINS 失效
标题翻译：多模式离子交换色谱树脂
公开(公告)号：US20100009867A1
公开(公告)日：2010-01-14
申请号：US12517570
申请日：2008-01-10
申请人： Enrique Carredano , Bo-Lennart Johansson , Jean-Luc Maloisel
发明人： Enrique Carredano , Bo-Lennart Johansson , Jean-Luc Maloisel
IPC分类号： C40B30/04 , C40B50/14 , C40B40/14
CPC分类号： C40B30/04 , B01D15/1885 , B01D15/361 , B01D15/362 , B01D15/363 , B01D15/3847 , B01J20/3285 , B01J39/26 , B01J41/20 , B01J2220/54 , C40B40/14 , C40B50/00 , G01N30/466 , G01N30/96
摘要： The present invention relates to a method of preparing a library of resins which are useful in chromatography, which method comprises creating a diversity of multi-modal ion exchange resins; and providing the diversity in a parallel system in which each resin is presented separated from the other resin(s).
摘要翻译：本发明涉及一种制备可用于色谱法的树脂文件库的方法，该方法包括产生多种多模式离子交换树脂; 并且在与其它树脂分离出每种树脂的并行系统中提供多样性。

32. 发明授权

US07214321B2 Adsorption method and ligands 有权
标题翻译：吸附法和配体
公开(公告)号：US07214321B2
公开(公告)日：2007-05-08
申请号：US10312054
申请日：2001-07-16
申请人： Makonnen Belew , Bo-Lennart Johansson , Jean-Luc Maloisel
发明人： Makonnen Belew , Bo-Lennart Johansson , Jean-Luc Maloisel
IPC分类号： B01D15/04
CPC分类号： B01J20/3285 , B01J39/04 , B01J39/18
摘要： The invention relates to a method for removing a positively charged substance from an aqueous liquid (I) by contacting the liquid with a cation-exchanger (1), possibly followed by a subsequent desorption of said substance. The cation-exchanger is selected to be capable of (a) binding to said substance by cation-exchange in an aqueous liquid reference (II) at an ionic strength corresponding to 0.3 M NaCl and (b) permitting a break through capacity for said substance 3200%, such as 3300% or 3500%, of the break-through capacity of said substance for a reference cation-exchanger (2) containing sulphopropyl groups —CH2CH2CH2SO2O—. The cation exchange ligands have an at least bimodal function by comprising a cation exchanging group and a separate hydrogen-bonding atom. The invention also relates to a method for testing the appropriateness of a cation-exchanger for removing a substance from a liquid and novel cation exchangers.
摘要翻译：本发明涉及通过使液体与阳离子交换剂（1）接触，从而可以随后随后解吸所述物质从水性液体（I）中除去带正电荷的物质的方法。阳离子交换剂被选择为能够（a）在水性液体参考物（II）中以对应于0.3M NaCl的离子强度的阳离子交换结合所述物质，和（b）允许所述物质的破裂能力例如300％或3％以上500％以上的所述物质用于参考阳离子交换器的穿透能力的200％（2）含有磺基丙基-CH 2 CH 2 CH 2 SO 2 O-。阳离子交换配体通过包含阳离子交换基团和单独的氢键原子具有至少双峰功能。本发明还涉及一种用于测试用于从液体和新型阳离子交换剂中除去物质的阳离子交换器的适当性的方法。

33. 发明授权

US09266041B2 Chromatography ligand 有权
标题翻译：色谱配体
公开(公告)号：US09266041B2
公开(公告)日：2016-02-23
申请号：US13158780
申请日：2011-06-13
申请人： Bo-Lennart Johansson , Jean-Luc Maloisel , Hans J. Johansson , Asa Ek , Maria Holm , Jafar Saidzadeh
发明人： Bo-Lennart Johansson , Jean-Luc Maloisel , Hans J. Johansson , Asa Ek , Maria Holm , Jafar Saidzadeh
IPC分类号： C07K1/14 , B01D15/32 , B01D15/36 , B01D15/38 , B01J20/28 , B01J20/286 , B01J20/32 , B01J41/20 , C07K1/16 , C07K1/18 , C07K1/36 , C07K16/00 , C07K16/06
CPC分类号： B01D15/327 , B01D15/363 , B01D15/3804 , B01J20/28033 , B01J20/286 , B01J20/3219 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/16 , C07K1/18 , C07K1/36 , C07K16/00 , C07K16/065 , Y10T29/49826
摘要： The present invention relates to a chromatography ligand defined by the following formula R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
摘要翻译：本发明涉及由下式R1-R2-N（R3）-R4-R5定义的色谱配体，其中R1是取代或未取代的苯基; R2是包含0-4个碳原子的烃链; R3是包含1-3个碳原子的烃链; R4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜耦合的所述配体。根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。

34. 发明授权

US09044740B2 Purification of immunoglobulins 有权
标题翻译：免疫球蛋白的纯化
公开(公告)号：US09044740B2
公开(公告)日：2015-06-02
申请号：US12594002
申请日：2008-03-20
申请人： Jean-Luc Maloisel , Bo-Lennart Johansson
发明人： Jean-Luc Maloisel , Bo-Lennart Johansson
IPC分类号： C07K16/00 , C07H1/00 , B01D15/38 , B01D15/08 , B01J20/289 , B01D15/32 , B01J20/286 , B01J20/32 , C07K1/20 , B01J20/26 , B01D15/36
CPC分类号： B01J20/289 , B01D15/32 , B01D15/327 , B01D15/361 , B01D15/3804 , B01D15/3847 , B01J20/262 , B01J20/267 , B01J20/286 , B01J20/3212 , B01J20/3219 , B01J20/3251 , B01J20/3272 , B01J20/328 , B01J20/3285 , B01J2220/54 , B01J2220/58 , C07K1/20 , C07K16/00
摘要： The present invention relates to a separation matrix comprised of a porous or non-porous support to which ligands have been immobilized, wherein said ligands comprise at least one aliphatic sulfamide. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulins, such as IgG, Fab fragments, fusion proteins containing immunoglobulins etc, by adsorption to a separation matrix that comprises the aliphatic sulfamide ligands of the invention.
摘要翻译：本发明涉及由已经固定有配体的多孔或无孔载体组成的分离基质，其中所述配体包含至少一个脂族磺酰胺。本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到包含脂族磺酰胺配体的分离基质上分离免疫球蛋白如IgG，Fab片段，含有免疫球蛋白的融合蛋白等的方法的本发明。

35. 发明申请

US20070112178A1 Antibody purification 有权
标题翻译：抗体纯化
公开(公告)号：US20070112178A1
公开(公告)日：2007-05-17
申请号：US10589718
申请日：2005-02-24
申请人： Bo-Lennart Johansson , Hans Johansson , Anders Ljunglof , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Bo-Lennart Johansson , Hans Johansson , Anders Ljunglof , Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： C07K16/00 , C02F1/28
CPC分类号： C07K1/18 , B01D15/362 , B01D15/3809 , B01D15/3847 , B01J39/07 , B01J39/26 , B01J47/014 , C07K1/165 , B01D15/327
摘要： The present invention relates to a method of separating antibodies from contaminants in a solution, which method comprises contacting the solution with a chromatography resin comprised of a support to which multi-modal ligands have been immobilised, wherein a multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system. In one embodiment, the ring-forming atoms of the aromatic or hereoaromatic entity are selected among C, S or O, and the cation exchanging group is a weak cation exchanger. The present method may be used as a single step procedure or as a polishing step following a capture on a Protein A column.
摘要翻译：本发明涉及一种从溶液中的污染物中分离抗体的方法，该方法包括使该溶液与包含固定有多元配体的载体的色谱树脂接触，其中多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统。在一个实施方案中，芳族或非芳族实体的成环原子选自C，S或O，并且阳离子交换基团是弱阳离子交换剂。本方法可以用作单步骤程序或作为在蛋白A柱上捕获后的抛光步骤。

36. 发明授权

US08217118B2 Activated solid support and method 失效
标题翻译：活性固体支持和方法
公开(公告)号：US08217118B2
公开(公告)日：2012-07-10
申请号：US12094286
申请日：2006-11-22
申请人： Gunnar Glad , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Gunnar Glad , Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： C07D303/02 , C07H1/00 , C08F8/00
CPC分类号： B01J20/262 , B01J20/261 , B01J20/265 , B01J20/267 , B01J20/28016 , B01J20/286 , B01J20/287 , B01J20/3204 , B01J20/321 , B01J20/3212 , B01J20/3217 , B01J20/3219 , B01J20/3246 , B01J20/3248 , B01J20/3251 , B01J20/3253 , B01J20/3255 , B01J2220/54 , B01J2220/58
摘要： Disclosed is a method for activating a solid support material with epoxy groups and for immobilizing ligands thereon, utilizing phase transfer catalytic conditions. The method permits the introduction of epoxy groups and specific nucleophilic ligands on the support material with a high level of substitution. Furthermore, the invention provides a general method for immobilizing a ligand for use in a wide variety of chromatographic separation procedures such as ion exchange chromatography, hydrophobic interaction chromatography (HIC), reverse phase chromatography (RPC), or affinity chromatography.
摘要翻译：公开了一种利用相转移催化条件活化具有环氧基团的固体支持材料和固定配体的方法。该方法允许以高水平的取代在载体材料上引入环氧基团和特定的亲核配体。此外，本发明提供了用于固定配体以用于各种色谱分离方法如离子交换层析，疏水相互作用层析（HIC），反相色谱（RPC）或亲和层析中的一般方法。

37. 发明授权

US08648146B2 Activated solid support and method 有权
标题翻译：活性固体支持和方法
公开(公告)号：US08648146B2
公开(公告)日：2014-02-11
申请号：US13489492
申请日：2012-06-06
申请人： Gunnar Glad , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Gunnar Glad , Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： C08F8/00 , C07H1/00 , C07D303/02
CPC分类号： B01J20/262 , B01J20/261 , B01J20/265 , B01J20/267 , B01J20/28016 , B01J20/286 , B01J20/287 , B01J20/3204 , B01J20/321 , B01J20/3212 , B01J20/3217 , B01J20/3219 , B01J20/3246 , B01J20/3248 , B01J20/3251 , B01J20/3253 , B01J20/3255 , B01J2220/54 , B01J2220/58
摘要： Disclosed is a method for activating a solid support material with epoxy groups and for immobilizing ligands thereon, utilizing phase transfer catalytic conditions. The method permits the introduction of epoxy groups and specific nucleophilic ligands on the support material with a high level of substitution. Furthermore, the invention provides a general method for immobilizing a ligand for use in a wide variety of chromatographic separation procedures such as ion exchange chromatography, hydrophobic interaction chromatography (HIC), reverse phase chromatography (RPC), or affinity chromatography.
摘要翻译：公开了一种利用相转移催化条件活化具有环氧基团的固体支持材料和固定配体的方法。该方法允许以高水平的取代在载体材料上引入环氧基团和特定的亲核配体。此外，本发明提供了用于固定配体以用于各种色谱分离方法如离子交换层析，疏水相互作用层析（HIC），反相色谱（RPC）或亲和层析中的一般方法。

38. 发明授权

US07005071B2 Method of generating metal chelating affinity ligands 有权
标题翻译：产生金属螯合亲和配体的方法
公开(公告)号：US07005071B2
公开(公告)日：2006-02-28
申请号：US10699058
申请日：2003-10-31
申请人： Lars C. Andersson , Tesfai Gebru , Andreas Axén , Jean-Luc Maloisel
发明人： Lars C. Andersson , Tesfai Gebru , Andreas Axén , Jean-Luc Maloisel
IPC分类号： B01D15/08
CPC分类号： B01J45/00 , B01D15/3828 , B01J20/286 , B01J20/3251 , B01J20/3265 , C07C323/58 , C07D333/36
摘要： The present invention relates to a method of generating at least one polydentate metal chelating affinity ligand, which method comprises the steps of a) providing at least one cyclic scaffold comprising a carbonyl, an adjacent sulphur and a nucleophile; b) providing at least one polydentate metal chelating affinity ligand arm, optionally in a form wherein the metal chelating functionalities are protected, on each scaffold by derivatisation of the nucleophile of said scaffolds, while retaining the cyclic structure of the scaffold; (c) ring-opening at the bond between the carbonyl and the sulphur of the derivatized scaffold by adding a reagent that adds one or more metal chelating affinity ligand arms to the scaffold; and, if required, (d) deprotecting the functionalities of the ligand arm(s) provided in step (b). In the most preferred embodiment of the method, steps (c) and (d) are performed simultaneously as one single step.
摘要翻译：本发明涉及产生至少一种多齿金属螯合亲和配体的方法，该方法包括该步骤

39. 发明申请

US20050100878A1 Method for the quantitative determination of one or more compounds 审中-公开
标题翻译：定量测定一种或多种化合物的方法
公开(公告)号：US20050100878A1
公开(公告)日：2005-05-12
申请号：US10398439
申请日：2001-10-02
申请人： Bengt Bjellqvist , Jean-Luc Maloisel , Ronnie Palmgren , Jonas Astrom
发明人： Bengt Bjellqvist , Jean-Luc Maloisel , Ronnie Palmgren , Jonas Astrom
IPC分类号： G01N27/62 , G01N27/447 , G01N27/64 , G01N30/06 , G01N30/72 , G01N30/88 , G01N33/68 , C12Q1/00
CPC分类号： G01N33/6803
摘要： The present invention relates to a method for the quantitative determination of the amount of one or more biomolecules, such as proteins or polypeptides, in one or more samples by utilising sample unique tagging reagents. More specifically, the method comprises steps of providing at least two samples; reacting biomolecules present in each sample with a sample unique mass tagging reagent to provide sample unique mass tagged forms thereof; combining tagged forms present in each sample to provide a single sample; coseparating, from the resulting sample, a mix of mass tagged forms of each of said biomolecules into different fractions; subjecting, for each fraction, the mix to mass spectrometry to obtain a mass spectrum; and determining from signals in each mass spectrum, the amount of the biomolecule corresponding to the spectrum in at least one of said samples relative to the amount of the same biomolecule in at least one of the remaining samples. In an advantageous embodiment, the separation step is a gel electrophoresis step. In some cases, it may be advantageous to also include a step of digesting biomolecules, such as protein(s).
摘要翻译：本发明涉及通过利用样品独特标记试剂在一个或多个样品中定量测定一种或多种生物分子（例如蛋白质或多肽）的量的方法。更具体地，该方法包括提供至少两个样本的步骤; 使每个样品中存在的生物分子与样品独特的质量标签试剂反应，以提供样品独特的质量标签形式; 组合每个样品中存在的标记形式以提供单个样品; 从所得样品中分离出每种所述生物分子的质量标签形式的混合物到不同的级分; 对每个级分进行混合物进行质谱分析以获得质谱; 并且从每个质谱图中的信号确定与至少一个剩余样品中相同生物分子的量相对应的至少一个所述样品中的光谱的生物分子的量。在有利的实施方案中，分离步骤是凝胶电泳步骤。在一些情况下，还可以包括消化生物分子如蛋白质的步骤是有利的。

40. 发明授权

US07510657B2 Method of preparing affinity ligands 失效
标题翻译：制备亲和配体的方法
公开(公告)号：US07510657B2
公开(公告)日：2009-03-31
申请号：US10547569
申请日：2004-03-05
申请人： Jean-Luc Maloisel , Nicolas Thevenin
发明人： Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： B01D15/08 , B01J20/22
CPC分类号： B01J20/286 , B01D15/3804 , B01J20/321 , B01J20/3212 , B01J20/3219 , B01J20/3251 , B01J20/3255 , B01J20/3259
摘要： The present invention is a method of preparing affinity ligands, which method comprises to provide a cyclic scaffold comprising a thiol group, a carbonyl group and an amine group; to derivatize the amine group of said scaffold with an electrophile that carries either a functionality capable of affinity interaction or a functionality capable of a second interaction with a target molecule; and to open up the derivatized scaffold and add an amine that carries either a functionality capable of a second interaction or a functionality capable of affinity interaction with a target molecule. The method provides provide affinity ligands, which are capable of affinity interaction as well as a second kind of interaction.
摘要翻译：本发明是制备亲和配体的方法，该方法包括提供包含硫醇基，羰基和胺基的环状支架; 以亲电子试剂衍生所述支架的胺基团，所述亲电子试剂携带能够进行亲和相互作用的官能团或能够与靶分子进行第二相互作用的官能团; 并开启衍生的支架并加入携带能够进行第二相互作用的官能团或能够与靶分子亲和相互作用的官能团的胺。该方法提供了提供亲和力配体，其能够进行亲和力相互作用以及第二种相互作用。

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