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31. 发明申请

US20050153308A1 Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil 失效
标题翻译：用于多胺催化亚硫酸氢盐转化胞嘧啶至尿嘧啶的方法和材料
公开(公告)号：US20050153308A1
公开(公告)日：2005-07-14
申请号：US10926530
申请日：2004-08-26
申请人： Gerald Zon , Victoria Boyd
发明人： Gerald Zon , Victoria Boyd
IPC分类号： C07H19/06 , C07H21/02 , C07H21/04 , C12Q20060101 , C12Q1/68
CPC分类号： C07H21/02 , C07H19/06 , C07H21/04 , C12Q1/68 , C12Q1/6806 , C12Q2523/125
摘要： The invention provides methods and materials for conversion of cytosine to uracil. In some embodiments, a nucleic acid, such as gDNA, is reacted with bisulfite and a polyamine catalyst, such as a triamine or tetra-amine. Optionally, the bisulfite comprises magnesium bisulfite. In other embodiments, a nucleic acid is reacted with magnesium bisulfite, optionally in the presence of a polyamine catalyst and/or a quaternary amine catalyst. Also provided are kits that can be used to carry out methods of the invention.
摘要翻译：本发明提供了将胞嘧啶转化成尿嘧啶的方法和材料。在一些实施方案中，核酸如gDNA与亚硫酸氢盐和多胺催化剂如三胺或四胺反应。任选地，亚硫酸氢盐包括亚硫酸氢钠。在其它实施方案中，核酸与亚硫酸氢镁反应，任选地在多胺催化剂和/或季胺催化剂的存在下反应。还提供了可用于实施本发明方法的试剂盒。

32. 发明申请

US20050095623A1 Method and materials for bisulfite conversion of cytosine to uracil 失效
标题翻译：亚硫酸氢盐转化为尿嘧啶的方法和材料
公开(公告)号：US20050095623A1
公开(公告)日：2005-05-05
申请号：US10926531
申请日：2004-08-26
申请人： Gerald Zon , Victoria Boyd
发明人： Gerald Zon , Victoria Boyd
IPC分类号： C07H20060101 , C07H21/02 , C07H21/04 , C12Q1/68
CPC分类号： C07H21/02 , C07H21/04 , C12Q1/6886 , C12Q2600/154 , Y10S435/81 , Y10S436/808
摘要： The invention provides methods and materials for conversion of cytosine to uracil. In some embodiments, a nucleic acid, such as gDNA, is reacted with at least one bisulfite salt having the formula X+HSO3− or Y+2(HSO331)2; wherein X+ is ammonium ion, a tetraalkyl ammonium ion, or a group 1A ion other than sodium; and Y+2 is a group 2A ion or a group 7B ion; under conditions effective to convert at least one cytosine nucleobase to a uracil nucleobase. In some embodiments, X+ comprises at least one of lithium ion, potassium ion, ammonium ion, tetraalkylammonium ion, magnesium ion, manganese ion and calcium ion. In some embodiments, the reacting is performed optionally in the presence of a polyamine catalyst and/or a quaternary amine catalyst. Also provided are kits that can be used to carry out methods of the invention.
摘要翻译：本发明提供了将胞嘧啶转化成尿嘧啶的方法和材料。在一些实施方案中，核酸，例如gDNA，与至少一种具有下式的亚硫酸氢盐反应：具有式X + 3，...，...， 2 + 2（HSO 3 3）3）2。其中X +为铵离子，四烷基铵离子或除钠以外的1A族离子; Y 2 + 2是2A族离子或7B族离子; 在有效将至少一个胞嘧啶核碱基转化为尿嘧啶核碱基的条件下。在一些实施方案中，X + +包括锂离子，钾离子，铵离子，四烷基铵离子，镁离子，锰离子和钙离子中的至少一种。在一些实施方案中，反应任选在多胺催化剂和/或季胺催化剂的存在下进行。还提供了可用于实施本发明方法的试剂盒。

33. 发明申请

US20050079527A1 Bisulfite method 失效
标题翻译：亚硫酸氢盐法
公开(公告)号：US20050079527A1
公开(公告)日：2005-04-14
申请号：US10926534
申请日：2004-08-26
申请人： Victoria Boyd , Gerald Zon
发明人： Victoria Boyd , Gerald Zon
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/6806 , C12Q2523/125
摘要： The invention provides methods for purifying bisulfite-treated nucleic acid samples.
摘要翻译：本发明提供了纯化亚硫酸氢盐处理的核酸样品的方法。

34. 发明授权

US06175006B1 Automated system for polynucleotide synthesis and purification 失效
标题翻译：用于多核苷酸合成和纯化的自动化系统
公开(公告)号：US06175006B1
公开(公告)日：2001-01-16
申请号：US09281205
申请日：1999-03-30
申请人： William A. Andrus , Christie D. McCollum , Gerald Zon
发明人： William A. Andrus , Christie D. McCollum , Gerald Zon
IPC分类号： C07H2100
CPC分类号： C07H21/00 , B01J19/0046 , B01J2219/00286 , B01J2219/00351 , B01J2219/00389 , B01J2219/00454 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/0068 , B01J2219/00686 , B01J2219/00722 , C12N15/1006 , C40B40/06 , C40B50/14 , C40B60/14
摘要： A method and system for polynucleotide synthesis are provided which employ solid phase synthesis on a nonswellable porous polystyrene support by phosphoramidite or hydrogen phosphonate chemistries. The polystyrene support gives rise to fewer tritylated failure sequences caused by chain growth from extraneous support sites, and allows lower amounts of monomer reactants to be used to achieve equal or better coupling efficiencies as those achieveable with CPG. The method and system also employ nucleoside intermediates whose exocyclic amines are protected by base-labile groups which permit simultaneous cleavage and deprotection of the completed polynucleotide chain in the presence of the solid phase support. This latter feature allows practical automation of both the synthesis and purification of polynucleotides.
摘要翻译：提供了一种用于多核苷酸合成的方法和系统，其通过亚磷酰胺或氢化膦酸盐化学物质在不可分散的多孔聚苯乙烯载体上进行固相合成。聚苯乙烯支持物产生由来自外部支持位点的链增长引起的三苯甲基化失败序列的更少，并且允许使用较少量的单体反应物来实现与CPG可达到的相同或更好的偶联效率。该方法和系统还使用核苷中间体，其外环胺由碱不稳定基团保护，其允许在固相载体存在下同时裂解和脱保护完整的多核苷酸链。后一个特征允许多核苷酸的合成和纯化的实际自动化。

35. 发明授权

US5262530A Automated system for polynucleotide synthesis and purification 失效
标题翻译：用于多核苷酸合成和纯化的自动化系统
公开(公告)号：US5262530A
公开(公告)日：1993-11-16
申请号：US558747
申请日：1990-07-27
申请人： William A. Andrus , Christie D. McCollum , Gerald Zon
发明人： William A. Andrus , Christie D. McCollum , Gerald Zon
IPC分类号： B01J19/00 , C07B61/00 , C07H21/00 , C12N15/10 , C40B40/06 , C40B50/14 , C40B60/14 , C07H21/02 , C07H21/04
CPC分类号： C07H21/00 , B01J19/0046 , C12N15/1006 , B01J2219/00286 , B01J2219/00351 , B01J2219/00389 , B01J2219/00454 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/0068 , B01J2219/00686 , B01J2219/00722 , C40B40/06 , C40B50/14 , C40B60/14
摘要： A method and system for polynucleotide synthesis are provided which employ solid phase synthesis on a nonswellable porous polystyrene support by phosphoramidite or hydrogen phosphonate chemistries. The polystyrene support gives rise to fewer tritylated failure sequences caused by chain growth from extraneous support sites, and allows lower amounts of monomer reactants to be used to achieve equal or better coupling efficiencies as those achieveable with CPG. The method and system also employ nucleoside intermediates whose exocyclic amines are protected by base-labile groups which permit simultaneous cleavage and deprotection of the completed polynucleotide chain in the presence of the solid phase support. This latter feature allows practical automation of both the synthesis and purification of polynucleotides.
摘要翻译：提供了一种用于多核苷酸合成的方法和系统，其通过亚磷酰胺或氢化膦酸盐化学物质在不可分散的多孔聚苯乙烯载体上进行固相合成。聚苯乙烯支持物产生由来自外部支持位点的链增长引起的三苯甲基化失败序列的更少，并且允许使用较少量的单体反应物来实现与CPG可达到的相同或更好的偶联效率。该方法和系统还使用核苷中间体，其外环胺由碱不稳定基团保护，其允许在固相载体存在下同时切割和脱保护完整的多核苷酸链。后一个特征允许多核苷酸的合成和纯化的实际自动化。

36. 发明授权

US5047524A Automated system for polynucleotide synthesis and purification 失效
标题翻译：用于多核苷酸合成和纯化的自动化系统
公开(公告)号：US5047524A
公开(公告)日：1991-09-10
申请号：US287890
申请日：1988-12-21
申请人： William A. Andrus , Christie D. McCollum , Gerald Zon
发明人： William A. Andrus , Christie D. McCollum , Gerald Zon
IPC分类号： C07H21/04 , B01J19/00 , C07B61/00 , C07H1/02 , C07H21/00 , C08L25/00 , C08L25/04 , C08L25/06 , C12N15/10 , C40B40/06 , C40B50/14 , C40B60/14
CPC分类号： C07H21/00 , B01J19/0046 , C12N15/1006 , B01J2219/00286 , B01J2219/00389 , B01J2219/00454 , B01J2219/005 , B01J2219/0059 , B01J2219/00596 , B01J2219/0068 , B01J2219/00686 , B01J2219/00722 , C40B40/06 , C40B50/14 , C40B60/14
摘要： A method and system for polynucleotide synthesis and purification are provided which employ solid phase synthesis on a nonswellable porous polystyrene support by phosphoramidite or hydrogen phosphonate chemistries. The polystyrene support gives rise to fewer tritylated failure squences caused by chain growth from extraneous support sites. Consequently, currently used rapid purification techniques depending on trityl hydrophobicity give a more highly purified product. The method and system also employ nucleoside intermediates whose exocyclic amines are protected by base-labile groups which permit simultaneous cleavage and deprotection of the completed polynucleotide chain in the presence of the solid phase support. This latter feature allows practical automation of both the synthesis and purification of polynucleotides.

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