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31. 发明授权

US07378261B2 Method for preparing para-hydroxystyrene by biocatalytic decarboxylation of para-hydroxycinnamic acid in a biphasic reaction medium 失效
标题翻译：在双相反应介质中通过对羟基肉桂酸的生物催化脱羧制备对羟基苯乙烯的方法
公开(公告)号：US07378261B2
公开(公告)日：2008-05-27
申请号：US10824581
申请日：2004-04-14
申请人： Arie Ben-Bassat , Sharon L. Haynie , David J. Lowe , Lisa L. Huang
发明人： Arie Ben-Bassat , Sharon L. Haynie , David J. Lowe , Lisa L. Huang
IPC分类号： C12P7/22 , C12N9/88 , C12N5/10 , C12N1/21 , C12N15/00 , C12N11/00 , C12P21/06
CPC分类号： C12P7/22 , C12P7/42
摘要： A biocatalytic method for preparing para-hydroxystyrene from para-hydroxycinnamic acid is described. The method uses an enzyme source having para-hydroxycinnamic acid decarboxylase activity to catalyze the decarboxylation of para-hydroxycinnamic acid in a biphasic reaction medium to produce para-hydroxystyrene, which is extracted into the organic phase of the biphasic reaction medium. The method results in a high yield of para-hydroxystyrene due to the decreased exposure of the enzyme source to the inhibitory product. The product is readily recovered from the extractant, or may be chemically derivatized directly in the extractant before recovery.
摘要翻译：描述了由对羟基肉桂酸制备对羟基苯乙烯的生物催化方法。该方法使用具有对羟基肉桂酸脱羧酶活性的酶源来催化双相反应介质中对羟基肉桂酸的脱羧作用，产生对羟基苯乙烯，将其提取到双相反应介质的有机相中。该方法由于酶源暴露于抑制产物而导致对羟基苯乙烯的高产率。该产物容易从萃取剂中回收，或者可以在回收之前在萃取剂中直接化学衍生化。

32. 发明授权

US06812000B2 Product removal process for use in a biofermentation system 失效
标题翻译：用于生物发酵系统的产品去除过程
公开(公告)号：US06812000B2
公开(公告)日：2004-11-02
申请号：US10127862
申请日：2002-04-22
申请人： Antoinette E. Wilkins , David J. Lowe
发明人： Antoinette E. Wilkins , David J. Lowe
IPC分类号： C12P100
CPC分类号： C12M47/12 , B01D15/1814 , B01D15/426 , C12M29/02 , C12M33/14 , C12M47/10 , C12N1/38 , C12P7/18
摘要： This invention relates to a bioprocess engineering solution for a product removal process for use in a biofermentation. The invention discloses a process for withdrawing an aliquot of broth from a biofermentation vessel during at least a portion of the biofermentation, removing biocatalyst and water, chromatographically separating biofermentation products from the withdrawn broth using water as an eluent, and returning the remaining components of the broth back to the biofermentation vessel. Process chromatography permits highly selective separation of the target molecule, preventing feedback inhibition of the biofermentation.
摘要翻译：本发明涉及用于生物发酵的产品去除方法的生物工艺工程解决方案。本发明公开了一种在至少一部分生物发酵过程中从生物发酵容器中取出等份的肉汤的方法，除去生物催化剂和水，使用水作为洗脱剂，使生物发酵产物与取出的肉汤色谱分离，并返回剩余组分肉汤回到生物发酵罐。过程色谱法允许目标分子的高度选择性分离，防止反应抑制生物发酵。

33. 发明授权

US5394485A Method and apparatus for smoothed scaling of facsimile images 失效
标题翻译：用于平滑缩放传真图像的方法和装置
公开(公告)号：US5394485A
公开(公告)日：1995-02-28
申请号：US32222
申请日：1993-03-17
申请人： David J. Lowe , Trudy L. Benjamin , William S. Eaton
发明人： David J. Lowe , Trudy L. Benjamin , William S. Eaton
IPC分类号： H04N1/387 , G06T3/40 , H04N1/40 , G06K9/42 , G06K9/40
CPC分类号： H04N1/40068
摘要： Facsimile image-scaling and -smoothing method and apparatus are described. They scale a received facsimile image along a given axis to increase the printed image resolution therealong, while smoothing the scaled image to reduce visually objectionable ticks. They do so by analyzing received image data with respect to adjacent received lines having disparate pixel counts and by filling in pixels therebetween in accordance with predefined fill rules that are designed also to smooth the output image to be printed. Various predefined rules are applied to produce intermediate results some or all of which are comprehended by the scaling/smoothing algorithm to produce the final smoothed, scaled output pixel image. Preferably such analyzing and filling in accordance with such rules are performed by a hybrid hardware and firmware subsystem within a receiving facsimile machine or facsimile-receiving printer.
摘要翻译：描述了传真图像缩放和平滑方法和装置。它们沿着给定的轴缩放接收到的传真图像以增加打印的图像分辨率，同时平滑缩放的图像以减少视觉上令人反感的刻度。它们通过分析相对于具有不同像素计数的相邻接收线的接收图像数据，并且根据预定义的填充规则填充其中的像素来进行，这些预定填充规则也被设计成平滑待印刷的输出图像。应用各种预定义规则来产生中间结果，其中一些或全部由缩放/平滑算法理解以产生最终平滑的，缩放的输出像素图像。优选地，根据这种规则的分析和填充由接收传真机或传真机接收打印机内的混合硬件和固件子系统执行。

34. 发明授权

US4795169A Radially stable mechanical face seals 失效
标题翻译：径向稳定的机械面密封
公开(公告)号：US4795169A
公开(公告)日：1989-01-03
申请号：US68661
申请日：1987-07-01
申请人： David J. Lowe , John Kemp
发明人： David J. Lowe , John Kemp
IPC分类号： F16J15/34 , F16J15/38
CPC分类号： F16J15/3464 , Y10S277/93
摘要： A mechanical face seal has a first seal face member mounted in fixed rotational and radial relationship to one component and sealed with respect thereto; abutment means mounted in fixed rotational and radial relationship to a second component and sealed with respect thereto; and a second seal face member interposed between the first seal face member and the abutment means. Spring means is arranged to apply an axial load between the first and second seal face members, said second seal face member having opposed faces, one of said faces being maintained in sealing engagement with a corresponding face on the first seal face member under the axial load applied by the spring means and the other face being sealed with respect to a corresponding face on said abutment means. Means is provided to interconnect said second seal face member rotationally with respect to said abutment means while permitting limited radial movement therebetween and one of said faces of the second seal face member and the corresponding face of the first seal face member or abutment means is inclined to the axis of the seal, so as to impose a radial load on the second face member. Two such seal assemblies may be provided co-axially of one another to provide a double seal.
摘要翻译：机械表面密封件具有第一密封面构件，其以一个部件固定的旋转和径向关系安装并相对于其密封; 邻接装置以与第二部件固定的旋转和径向关系安装并相对于其密封; 以及插入在第一密封面构件和抵接装置之间的第二密封面构件。弹簧装置布置成在第一和第二密封面构件之间施加轴向载荷，所述第二密封面构件具有相对的面，所述面中的一个在轴向载荷下与第一密封面构件上的相应面保持密封接合通过弹簧装置施加，另一个面相对于所述邻接装置上的相应面密封。提供装置，用于将所述第二密封面构件相对于所述邻接装置旋转地互相连接，同时允许其间的有限的径向运动，并且第二密封面构件的所述面和第一密封面构件或邻接构件的相应面之一倾斜于密封件的轴线，以便在第二面构件上施加径向载荷。两个这样的密封组件可以彼此同轴地设置以提供双重密封。

35. 发明授权

US4759554A Mechanical face seals 失效
标题翻译：机械表面密封
公开(公告)号：US4759554A
公开(公告)日：1988-07-26
申请号：US924454
申请日：1986-10-29
申请人： John Kemp , David J. Lowe
发明人： John Kemp , David J. Lowe
IPC分类号： F16J15/34 , F16J15/38
CPC分类号： F16J15/3464
摘要： A mechanical face seal comprises a first seal face member associated in fixed relationship and sealed with respect to one component, a second seal face member associated in fixed rotational relationship and sealed with respect to a second component and a third seal face member interposed between the first and second seal face members. Springs are provided to urge the second seal face member towards the first seal face member and maintain the first, second and third seal face members in sealing engagement. The sealing faces of the first, second and third seal face members are inclined, so that the first and second seal face members impose a radial load on the third seal face member. Two such seal assemblies may be provided co-axially of one another to provide a double seal.
摘要翻译：机械表面密封件包括固定关系并相对于一个部件密封的第一密封面部件，以固定旋转关系相关联并且相对于第二部件密封的第二密封面部件，以及插入在第一部件之间的第三密封面部件和第二密封面构件。提供弹簧以将第二密封面构件推向第一密封面构件并且将第一密封面构件和第三密封面构件保持密封接合。第一，第二和第三密封面构件的密封面倾斜，使得第一和第二密封面构件在第三密封面构件上施加径向载荷。两个这样的密封组件可以彼此同轴地设置以提供双重密封。

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