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21. 发明申请

US20120171722A1 PERSONALIZED PRODUCTION OF BIOLOGICS AND METHOD FOR REPROGRAMMING SOMATIC CELLS 有权
标题翻译：生物学的个性化生产和用于重建神经细胞的方法
公开(公告)号：US20120171722A1
公开(公告)日：2012-07-05
申请号：US13342704
申请日：2012-01-03
申请人： Theresa A. Deisher
发明人： Theresa A. Deisher
IPC分类号： C12P21/00 , C12N9/26 , C12N9/40 , C12N9/74 , C12N9/24
CPC分类号： C12N5/0696 , A61K35/545 , A61K38/00 , A61K39/00 , C07K14/00 , C07K16/00 , C07K2317/14 , C07K2317/41 , C12N2510/02 , C12N2517/04
摘要： The invention provides a method for producing polypeptide protein products and nucleic acid products having reduced levels of antigenicity in an animal being treated with a biologic product. Somatic cells are isolated from an animal, transformed into pluripotent stem cells, transfected with a nucleic acid(s) of interest, and re-differentiated towards somatic cells known to be high level producers of the desired nucleic acid product. The invention can be used to derive a general cell line to treat populations, racial specific cell lines to treat ethnic groups, or patient specific cell lines to treat individuals. Additionally, the invention provides a method to allow induced pluripotent stem cells to be re-differentiated towards their somatic cell of origin so that the cells can be used to therapeutically treat an animal without resulting teratoma formation.
摘要翻译：本发明提供了用生物制品处理的动物中产生具有降低的抗原性水平的多肽蛋白产物和核酸产物的方法。将体细胞从动物中分离，转化成用目标核酸转染的多能干细胞，并再次分化为已知是所需核酸产物的高水平生产者的体细胞。本发明可用于衍生一般细胞系以治疗群体，种族特异性细胞系以治疗种族群体或患者特异性细胞系以治疗个体。另外，本发明提供了允许诱导的多能干细胞对其体细胞原体再分化的方法，使得细胞可用于治疗性处理动物而不导致畸胎瘤形成。

22. 发明授权

US08062876B2 Method for activating prethrombin-1 有权
标题翻译：激活凝血酶原-1的方法
公开(公告)号：US08062876B2
公开(公告)日：2011-11-22
申请号：US12097027
申请日：2006-12-21
申请人： Robert W. Mallett , Christopher J. Stenland , Jonathon C. Boone , John W. Forstrom , Karen S. De Jongh
发明人： Robert W. Mallett , Christopher J. Stenland , Jonathon C. Boone , John W. Forstrom , Karen S. De Jongh
IPC分类号： C12N9/74
CPC分类号： C12N9/6429 , C12Y304/21005
摘要： Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.
摘要翻译：公开了将凝血酶原-1转化为凝血酶的方法。将凝血酶原-1的水溶液施加到固定在固体支持物上的西葫芦C上，从而提供每毫升固体支持物500mg至4000mg的凝血酶原-1和凝血酶原-1与西葫芦之间的接触时间 -C为1.8至3.5分钟。所得到的活性凝血酶可以在离子交换色谱介质或亲和层析介质上捕获。

23. 发明授权

US08043629B2 Bioabsorbable synthetic nonwoven fabric holding thrombin 有权
标题翻译：生物可吸收的合成无纺布保持凝血酶
公开(公告)号：US08043629B2
公开(公告)日：2011-10-25
申请号：US11941779
申请日：2007-11-16
申请人： Takanori Uchida , Noriko Shinya , Hiroshi Kaetsu , Takayuki Imamura , Chikateru Nozaki
发明人： Takanori Uchida , Noriko Shinya , Hiroshi Kaetsu , Takayuki Imamura , Chikateru Nozaki
IPC分类号： A61L15/32 , A61K9/70 , A61K38/48 , C12N9/74
CPC分类号： A61L15/44 , A61K47/6953 , A61L15/26 , A61L15/32 , A61L15/64 , A61L33/0041 , A61L2300/418 , A61L2400/04 , Y10T442/2525 , Y10T442/2566 , C08L67/04
摘要： A safe and effective hemostatic is provided. The invention relates to a bioabsorbable synthetic nonwoven fabric holding thrombin as an effective ingredient and a hemostatic comprising said bioabsorbable synthetic nonwoven fabric. The bioabsorbable synthetic nonwoven fabric holding thrombin in accordance with the present invention may be prepared by a process which comprises the steps of immersing a bioabsorbable synthetic nonwoven fabric into a solution containing thrombin and of lyophilizing the obtained nonwoven fabric. The bioabsorbable synthetic nonwoven fabric holding thrombin in accordance with the present invention allows for quicker and more effective hemostasis.
摘要翻译：提供安全有效的止血剂。本发明涉及一种保持凝血酶作为有效成分的生物可吸收合成无纺布和包含所述生物可吸收合成无纺织物的止血剂。根据本发明的保持凝血酶的生物吸收性合成无纺布可以通过包括以下步骤的方法制备，所述方法包括将生物可吸收的合成非织造织物浸入含有凝血酶的溶液中并将所得的无纺织物冻干。根据本发明的保持凝血酶的生物可吸收合成非织造织物可以更快更有效地止血。

24. 发明授权

US07807351B2 Method of detecting target molecule by using aptamer 有权
标题翻译：通过使用适体检测靶分子的方法
公开(公告)号：US07807351B2
公开(公告)日：2010-10-05
申请号：US10580044
申请日：2004-11-22
申请人： Koji Sode , Kazunori Ikebukuro
发明人： Koji Sode , Kazunori Ikebukuro
IPC分类号： C12Q1/68 , C12N9/74
CPC分类号： G01N33/54393 , C12Q1/6816 , G01N33/543 , C12Q2525/161 , C12Q2525/205 , C12Q2565/107
摘要： An aptamer-probe complex for detecting the presence of a target molecule is disclosed. The complex of the present invention contains an aptamer moiety which is able to bind to an indicator protein and change the properties of the indicator protein, and a probe moiety which is able to bind to a target molecule, wherein the aptamer moiety and the probe moiety are combined in such a manner that the binding mode between the aptamer moiety and the indicator protein changes when the probe moiety binds to the target molecule. A target molecule can be detected with combination of an aptamer which binds to a certain protein, and a probe which binds to the target molecule, utilizing the properties of that protein as an indicator.
摘要翻译：公开了一种用于检测靶分子存在的适体 - 探针复合物。本发明的复合物包含能够结合指示蛋白并改变指示蛋白的性质的适体部分，以及能够结合靶分子的探针部分，其中适体部分和探针部分以使得当探针部分结合到靶分子时，适体部分和指示蛋白之间的结合模式发生变化的方式组合。可以通过结合某种蛋白质的适配体的组合和结合靶分子的探针，利用该蛋白质的特性作为指标，检测靶分子。

25. 发明申请

US20100233783A1 NOVEL METHOD FOR PROTEIN PURIFICATION 审中-公开
标题翻译：用于蛋白质纯化的新方法
公开(公告)号：US20100233783A1
公开(公告)日：2010-09-16
申请号：US11909550
申请日：2006-03-23
申请人： Johan Per Henrik Svensson , Leif Bülow
发明人： Johan Per Henrik Svensson , Leif Bülow
IPC分类号： C07K1/14 , C07K14/575 , C07K14/765 , C07K14/62 , C07K14/585 , C07K7/14 , C07K14/665 , C07K14/77 , C12N9/92 , C12N9/26 , C07K14/555 , C07K14/755 , C07K14/78 , C12N9/74 , C12N15/63 , C12N1/21 , C07K14/00
CPC分类号： C07K1/14
摘要： Process for purifying a recombinant protein including one or a few procedural steps only. The process combines the step of lysis of the host cell, with the purification of the protein of interest, allowing for a rapid and much more efficient process of purification. The conditions used during the purification process are those of a high temperature and a low pH, allowing for thermostable and acid-resistant recombinant proteins to be isolated from a suspension. The invention also relates to purifying recombinant proteins which are fusion proteins, wherein one part of the protein may be selected from an enamel matrix protein, such as amelogenin.
摘要翻译：纯化重组蛋白质的方法，包括一个或几个程序步骤。该方法将宿主细胞的裂解步骤与目的蛋白质的纯化相结合，从而允许快速且更有效的纯化方法。纯化过程中使用的条件是高温和低pH的条件，允许从悬浮液中分离出耐热和耐酸的重组蛋白。本发明还涉及纯化作为融合蛋白的重组蛋白质，其中一部分蛋白质可以选自釉质基质蛋白质，例如am en。。。。。。。。。。。。

26. 发明申请

US20090137001A1 PROCESS FOR PREPARING RECOMBINANT HUMAN THROMBIN WITH CULTURE CELL 审中-公开
标题翻译：用文化细胞制备重组人血红蛋白的方法
公开(公告)号：US20090137001A1
公开(公告)日：2009-05-28
申请号：US12088823
申请日：2006-09-29
申请人： Tatsufumi Onchi , Yo Nakahara , Takayuki Imamura , Toshinobu Nouchi , Hiroshi Nakatake
发明人： Tatsufumi Onchi , Yo Nakahara , Takayuki Imamura , Toshinobu Nouchi , Hiroshi Nakatake
IPC分类号： C12P21/02 , C12N9/74 , C07K14/745 , C12N5/06
CPC分类号： C12N9/6429 , C12Y304/21005
摘要： The present invention provides for a process for preparing a recombinant human thrombin. A process for preparing a recombinant human thrombin which comprises: (1) obtaining a transfectant cell producing a human prothrombin by introducing an expression vector, wherein a gene fragment coding for a human prothrombin gene is incorporated, into an animal cell; (2) purifying a human prothrombin from the culture of the transfectant cell above by an anion exchanger; (3) converting the purified human prothrombin into a human thrombin by subjecting said human prothrombin to the action of ecarin; and (4) purifying the human thrombin from the solution after treatment with ecarin by an affinity method using benzamidine and a cation exchanger, and human thrombin obtained by said process, and a CHO cell that produces human prothrombin.
摘要翻译：本发明提供了制备重组人凝血酶的方法。一种制备重组人凝血酶的方法，其包括：（1）通过将编码人凝血酶原基因的基因片段引入到动物细胞中的表达载体获得产生人凝血酶原的转染细胞; （2）通过阴离子交换剂从上述转染细胞的培养物纯化人凝血酶原; （3）通过使所述人凝血酶原受到ecarin的作用，将纯化的人凝血酶原转化为人凝血酶; 和（4）使用苯甲脒和阳离子交换剂的亲和法，通过所述方法获得的人凝血酶和产生人凝血酶原的CHO细胞，用ecarin处理后，从溶液中纯化人凝血酶。

27. 发明申请

US20090047273A1 Method For Production of Recombinant Human Thrombin ['644] 失效
标题翻译：重组人凝血酶的制备方法['644]
公开(公告)号：US20090047273A1
公开(公告)日：2009-02-19
申请号：US12167614
申请日：2008-07-03
申请人： Anna Harrysson , Ann Lovgren
发明人： Anna Harrysson , Ann Lovgren
IPC分类号： A61K38/48 , C12P21/04 , C12N15/11 , C12N5/06 , A61P7/00 , C12N15/00 , C12N9/74
CPC分类号： C12N9/6418 , C12N9/6429 , C12Y304/21005
摘要： The present invention relates to a method is provided for producing recombinant human thrombin from recombinant prothrombin using recombinant ecarin having the sequence SEQ ID NO 2 or a homologue thereof.
摘要翻译：本发明涉及使用具有序列SEQ ID NO 2的重组ecarin或其同源物从重组凝血酶原制备重组人凝血酶的方法。

28. 发明申请

US20080254446A1 Method of Detecting Target Molecule by Using Aptamer 有权
标题翻译：使用适配器检测目标分子的方法
公开(公告)号：US20080254446A1
公开(公告)日：2008-10-16
申请号：US10580044
申请日：2004-11-22
申请人： Koji Sode , Kazunori Ikebukuro
发明人： Koji Sode , Kazunori Ikebukuro
IPC分类号： C12Q1/68 , C12N9/74
CPC分类号： G01N33/54393 , C12Q1/6816 , G01N33/543 , C12Q2525/161 , C12Q2525/205 , C12Q2565/107
摘要： An aptamer-probe complex for detecting the presence of a target molecule is disclosed. The complex of the present invention contains an aptamer moiety which is able to bind to an indicator protein and change the properties of the indicator protein, and a probe moiety which is able to bind to a target molecule, wherein the aptamer moiety and the probe moiety are combined in such a manner that the binding mode between the aptamer moiety and the indicator protein changes when the probe moiety binds to the target molecule. A target molecule can be detected with combination of an aptamer which binds to a certain protein, and a probe which binds to the target molecule, utilizing the properties of that protein as an indicator.
摘要翻译：公开了一种用于检测靶分子存在的适体 - 探针复合物。本发明的复合物包含能够结合指示蛋白并改变指示蛋白的性质的适体部分，以及能够结合靶分子的探针部分，其中适体部分和探针部分以使得当探针部分结合到靶分子时，适体部分和指示蛋白之间的结合模式发生变化的方式组合。可以通过结合某种蛋白质的适配体的组合和结合靶分子的探针，利用该蛋白质的特性作为指标来检测靶分子。

29. 发明授权

US07005271B1 Method for the determination of the prethrombotic state 失效
标题翻译：测定血栓前状态的方法
公开(公告)号：US07005271B1
公开(公告)日：2006-02-28
申请号：US09588553
申请日：2000-06-07
申请人： Jean-Marie Freyssinet , Benedicte Antoni , Frederic Donie , Helmut Lill
发明人： Jean-Marie Freyssinet , Benedicte Antoni , Frederic Donie , Helmut Lill
IPC分类号： C07K14/745 , C12N9/74 , C12Q1/37 , G01N33/543 , G01N33/554
CPC分类号： G01N33/56966 , G01N33/86 , G01N33/92 , G01N2333/4718
摘要： The present invention relates to determining the prethrombotic state, in particular determining an amount or presence of circulating microparticles and/or stimulated procoagulant cells.
摘要翻译：本发明涉及确定预血栓形成状态，特别是确定循环微粒和/或刺激的促凝细胞的量或存在。

30. 发明申请

US20050227923A1 Stimulation of bone growth with thrombin peptide derivatives 失效
公开(公告)号：US20050227923A1
公开(公告)日：2005-10-13
申请号：US11038014
申请日：2005-01-18
申请人： Darrell Carney , Roger Crowther , David Simmons , Jinping Yang , William Redin
发明人： Darrell Carney , Roger Crowther , David Simmons , Jinping Yang , William Redin
IPC分类号： A61L27/00 , A61K38/00 , A61K38/10 , A61K38/16 , A61K38/18 , A61K38/46 , A61K38/48 , A61K45/00 , A61K47/04 , A61K47/34 , A61K47/42 , A61P19/00 , C12N9/74 , C12N9/76
CPC分类号： A61K38/10 , A61K38/16 , A61K38/4833 , A61L2430/02
摘要： Disclosed is a method of stimulating bone growth at a site in a subject in need of osteoinduction. The method comprises the step of administering a therapeutically effective amount of an agonist of the non-proteolytically activated thrombin receptor to the site.

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