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21. 发明授权

US07300790B2 Transgenic animals expressing transdominant negative retroviral nucleic acids and proteins 失效
标题翻译：转基因动物表达显性阴性逆转录病毒核酸和蛋白质
公开(公告)号：US07300790B2
公开(公告)日：2007-11-27
申请号：US10764201
申请日：2004-01-23
申请人： Kurt Eakle , Thomas Hope , Eun-A Choi , Jane Homan , Robert D. Bremel
发明人： Kurt Eakle , Thomas Hope , Eun-A Choi , Jane Homan , Robert D. Bremel
IPC分类号： C12P1/00 , C12P37/04
CPC分类号： C07K14/005 , A01K2217/05 , A01K2227/101 , A01K2267/02 , A61K48/00 , C12N2740/14022 , C12N2740/14043 , C12N2830/00
摘要： The present invention relates to non-vaccinal and non-pharmacologic compositions and methods for controlling complex retroviral infections. In particular, the present invention provides transgenic animals expressing a transdominant negative Rex gene product that inhibits retroviral replication.
摘要翻译：本发明涉及用于控制复合逆转录病毒感染的非疫苗和非药物组合物和方法。特别地，本发明提供表达抑制逆转录病毒复制的显性阴性Rex基因产物的转基因动物。

22. 发明授权

US6060268A Penicillin G acylase immobilized with a crosslinked mixture of gelled gelatin and amino polymer 失效
标题翻译：用胶凝明胶和氨基聚合物的交联混合物固定的青霉素G酰基转移酶
公开(公告)号：US6060268A
公开(公告)日：2000-05-09
申请号：US983370
申请日：1998-01-15
申请人： Erik De Vroom
发明人： Erik De Vroom
IPC分类号： C12N11/02 , C12N11/04 , C12N11/10 , C12P37/04 , C12P35/04
CPC分类号： C12N11/02 , C12N11/04 , C12N11/10
摘要： Penicillin G acylase is immobilized by covalent bonding to a crosslinked mixture of a gelled gelling agent such as gelatin and a polymer containing free amino groups such as alginate amine, chitosan or polyethylene imine. The immobilized penicillin G acylase provides a higher synthesis/hydrolysis ratio as compared to immobilizing with other carriers when producing .beta.-lactam derivatives by a condensing reaction of an amino .beta.-lactam with an acylating agent. The acylating agent may be a derivative of D-phenylglycine, a derivative of D-p-hydroxyphenylglycine or a derivative of D-2,5-dihydro-phenylglycine. Examples of .beta.-lactam derivatives that can be produced are amoxycillin, ampicillin, cephaclor, cephadroxil, cephprozil, cephalexin and cephradine.
摘要翻译： PCT No.PCT / EP96 / 03253 Sec。 371日期1998年1月15日 102（e）1998年1月15日PCT PCT 1996年7月16日PCT公布。出版物WO97 / 04086 日期1997年2月6日青霉素G酰基转移酶通过共价键固定到凝胶化胶凝剂如明胶和含有游离氨基的聚合物如藻酸盐，壳聚糖或聚乙烯亚胺的交联混合物中。固定化的青霉素G酰基转移酶与通过氨基β-内酰胺与酰化剂的缩合反应制备β-内酰胺衍生物时相比，与其它载体固定相比提供了更高的合成/水解比。酰化剂可以是D-苯基甘氨酸的衍生物，D-对羟基苯基甘氨酸的衍生物或D-2,5-二氢 - 苯基甘氨酸的衍生物。可以生产的β-内酰胺衍生物的实例是阿莫西林，氨苄青霉素，cephaclor，cephadroxil，cephprozil，头孢氨苄和头孢拉定。

23. 发明授权

US5559005A Bioprocess for preparing 7-ACA and 7-ADAC 失效
标题翻译：用于制备7-ACA和7-ADAC的生物工艺
公开(公告)号：US5559005A
公开(公告)日：1996-09-24
申请号：US250310
申请日：1994-05-27
申请人： Michael J. Conder , Phyllis C. McAda , John A. Rambosek , Christopher D. Reeves
发明人： Michael J. Conder , Phyllis C. McAda , John A. Rambosek , Christopher D. Reeves
IPC分类号： C12N1/19 , C07D501/20 , C12N1/15 , C12N9/00 , C12N9/02 , C12N9/10 , C12N15/09 , C12N15/52 , C12N15/54 , C12P20060101 , C12P35/00 , C12P35/06 , C12P37/04 , C12R1/82 , C12P35/04 , C12N1/16
CPC分类号： C12N9/0071 , C12N9/00 , C12N9/1029 , C12P35/00 , C12P37/04 , Y10S435/935
摘要： Important intermediates for preparing cephalosporin antibiotics, 7-amino-cephalosporanic acid (7-ACA) and 7-aminodeacetylcephalosporanic acid (7-ADAC), are prepared by a novel bioprocess in which a transformed Penicillium chrysogenum strain is cultured in the presence of an adipate feedstock to produce adipoyl-6-APA (6-amino penicillanic acid); followed by the in situ expression of the following genes with which the P. chrysogenum has been transformed:1) an expandase gene, e.g., from Cephalosporium acremonium, whose expression product converts the adipoyl-6-APA by ring expansion to adipoyl-7-ADCA;2) an hydroxylase gene whose expression product converts the 3-methyl side chain of adipoyl-7-ADCA to 3-hydroxymethyl, to give the first product, 7-aminodeacetylcephalosporanic acid (7-ADAC); and3) an acetyltransferase gene whose expression product converts the 3-hydroxymethyl side chain to the 3-acetyloxymethyl side chain of 7-ACA. The final product, 7-ACA, is then prepared by cleavage of the adipoyl side chain using an adipoyl acylase. The entire synthesis, accordingly, is carried out using bioprocesses, and is efficient and economical.
摘要翻译：用于制备头孢菌素抗生素，7-氨基头孢菌素酸（7-ACA）和7-氨基脱乙酰头孢菌酸（7-ADAC）的重要中间体是通过新型生物工艺制备的，其中转化的产黄青霉菌株在己二酸存在下培养生产己二酰-6-APA（6-氨基青霉烷酸）的原料; 其次是产生产黄青霉的以下基因的原位表达：1）扩增酶基因，例如头孢霉头孢菌，其表达产物通过环扩展将己二酰基-6-APA转化为己二酰-7- ADCA; 2）表达产物将己二酰-7-ADCA的3-甲基侧链转化为3-羟甲基的羟化酶基因，得到第一产物7-氨基乙酰基头孢烷酸（7-ADAC）; 和3）其表达产物将3-羟甲基侧链转化为7-ACA的3-乙酰氧甲基侧链的乙酰转移酶基因。然后通过使用己二酰酰化酶裂解己二酰侧链制备最终产物7-ACA。因此，整个合成使用生物工艺进行，并且是高效和经济的。

24. 发明授权

US5318896A Recombinant expandase bioprocess for preparing 7-aminodesacetoxy cephalosporanic acid (7-ADCA) 失效
标题翻译：用于制备7-氨基脱乙酰氧基头孢菌酸（7-ADCA）的重组扩环酶生物工艺
公开(公告)号：US5318896A
公开(公告)日：1994-06-07
申请号：US933469
申请日：1992-08-28
申请人： Michael J. Conder , Phyllis C. McAda , John A. Rambosek
发明人： Michael J. Conder , Phyllis C. McAda , John A. Rambosek
IPC分类号： C12N15/09 , C12N1/15 , C12N1/19 , C12N1/21 , C12N9/00 , C12N9/02 , C12N9/80 , C12N15/00 , C12N15/52 , C12N15/81 , C12P35/00 , C12P35/02 , C12P35/06 , C12P37/04 , C12R1/38 , C12R1/465 , C12R1/645 , C12R1/82 , C12N9/84
CPC分类号： C12N9/0071 , C12P35/00 , C12P37/04 , Y10S435/935
摘要： An important intermediate for preparing cephalosporin antibiotics, 7-aminodesacetoxy cephalosporanic acid (7-ADCA), is prepared by a novel bioprocess in which a transformed Penicillium chrysogenum strain is cultured in the presence of an adipate feedstock to produce adipoyl-6-APA (6-amino penicillanic acid); and the in situ expression of an expandase gene, e.g., from Streptomyces clavuligerus, with which the P. chrysogenum has been transformed, converts the adipoly-6-APA by ring expansion to adipoyl-7-ADCA. The final product, 7-ADCA, is then prepared by cleavage of the adipoyl side chain using an adipoyl acylase. The entire synthesis, accordingly, is carried out using bioprocesses, and is efficient and economical.
摘要翻译：通过一种新的生物工艺制备了头孢菌素类抗生素7-氨基乙酰氧基头孢菌素酸（7-ADCA）的重要中间体，其中转化的产黄青霉菌株在己二酸原料存在下培养以产生己二酰基-6-APA（6 氨基青霉烷酸）; 并且已经转化了产黄青霉的膨胀酶基因例如来自链霉菌的原位表达通过环扩展转化为己二酰-7-ADCA的adipoly-6-APA。然后通过使用己二酰酰化酶裂解己二酰侧链制备最终产物7-ADCA。因此，整个合成使用生物工艺进行，并且是高效和经济的。

25. 发明授权

US5268271A Method for the synthesis of semi-synthetic antibiotics in thermodynamically controlled water-cosolvent organic miscible apolar systems by using penicillin G acylase 失效
标题翻译：通过使用青霉素G酰基转移酶在热力学控制的水共溶剂有机混溶非极性体系中合成半合成抗生素的方法
公开(公告)号：US5268271A
公开(公告)日：1993-12-07
申请号：US767044
申请日：1991-08-02
申请人： Jose M. Guisan Seijas , Roberto Fernandez Lafuente , Gregorio Alvaro Campos , Rosa M. Blanco Martin , Cristina Molina Rosell
发明人： Jose M. Guisan Seijas , Roberto Fernandez Lafuente , Gregorio Alvaro Campos , Rosa M. Blanco Martin , Cristina Molina Rosell
IPC分类号： C12P35/04 , C12P37/04 , C12P37/00 , C12N9/84
CPC分类号： C12P35/04 , C12P37/04
摘要： Synthesis of semi-synthetic monobactamic or .beta.-Lactamic antibiotics by using derivatives stabilized by various methods of penicillin G acylase from various microbial sources according to a thermodynamically controlled strategy in monophase water/cosolvent organic apolar systems, wherein the concentration of the cosolvent varies between 30% and 90%, the temperature between -10.degree. C. and 50.degree. C., the pH between 4.5 and 8.5, with concentrations of the antibiotic nucleus between 0.5 an 875 mM and acyl donor between 0.2 mM and 1M, with a relationship antibiotic ring/activated or free acyl donor, using a buffer between 0 and 1M. Application to the pharmaceutical industry.
摘要翻译：通过使用通过各种微生物来源的各种青霉素G酰基转移酶稳定的衍生物，根据单相水/助溶剂有机非极性体系中的热力学控制策略合成半合成单体或β-乳酸抗生素，其中助溶剂的浓度在30 ％和90％，温度在-10℃和50℃之间，pH在4.5和8.5之间，抗生素核的浓度在0.5至875mM之间，酰基供体在0.2mM和1M之间，具有关系型抗生素环/活化或游离酰基供体，使用0至1M之间的缓冲液。适用于制药行业。

26. 发明授权

US4556559A Antibiotics 失效
标题翻译：抗生素
公开(公告)号：US4556559A
公开(公告)日：1985-12-03
申请号：US924174
申请日：1978-07-13
申请人： Martin Cole , Thomas T. Howarth , Christopher Reading
发明人： Martin Cole , Thomas T. Howarth , Christopher Reading
IPC分类号： A61K31/42 , A61K31/43 , A61P31/04 , C07D503/00 , C12N9/99 , C12P1/06 , C12P17/18 , C12P37/04 , A61K35/00
CPC分类号： C12P17/188 , A61K31/42 , A61K31/43 , C07D503/00
摘要： A new antibacterially active agent has been isolated from Streptomyces clvuligerus. This new compound which is designated clavulanic acid has the formula (I): ##STR1## In addition to being a broad spectrum antibiotic of medium potency, clavulanic acid and its salts and esters have the ability to enhance the effectiveness of .beta.-lactam antibiotics against many .beta.-lactamase producing bacteria.
摘要翻译：已经从Streptomyces clvuligerus中分离出新的抗菌活性剂。这种被称为克拉维酸的新化合物具有式（I）：（I）除了是广谱抗生素的中等效力之外，克拉维酸及其盐和酯还具有增强β - 针对许多β-内酰胺酶生产细菌的内酰胺抗生素。

27. 发明授权

US4525352A Antibiotics 失效
标题翻译：抗生素
公开(公告)号：US4525352A
公开(公告)日：1985-06-25
申请号：US327253
申请日：1981-12-03
申请人： Martin Cole , Thomas T. Howarth , Christopher Reading
发明人： Martin Cole , Thomas T. Howarth , Christopher Reading
IPC分类号： A61K31/42 , A61K31/43 , A61P31/04 , C07D503/00 , C12N9/99 , C12P1/06 , C12P17/18 , C12P37/04 , A61K35/00
CPC分类号： C12P17/188 , A61K31/42 , A61K31/43 , C07D503/00
摘要： A new antibacterially active agent has been isolated from Streptomyces clavuligerus. This new compound which is designated clavulanic acid has the formula (I): ##STR1## In addition to being a broad spectrum antibiotic of medium potency, clavulanic acid and its salts and esters have the ability to enhance the effectiveness of .beta. lactam antibotics against many .beta.-lactamase producing bacteria.
摘要翻译：已经从Streptomyces clavuligerus中分离出一种新的抗菌活性剂。这种被称为克拉维酸的新化合物具有式（I）：其中除了是广谱抗生素的中等效力外，克拉维酸及其盐和酯还具有提高β-内酰胺的有效性的能力针对许多β-内酰胺酶生产细菌的抗生素。

28. 发明授权

US4427690A Esters of clavulanic acid 失效
标题翻译：克拉维酸的酯
公开(公告)号：US4427690A
公开(公告)日：1984-01-24
申请号：US726224
申请日：1976-09-24
申请人： Martin Cole , Thomas T. Howarth , Christopher Reading
发明人： Martin Cole , Thomas T. Howarth , Christopher Reading
IPC分类号： A61K31/42 , A61K31/43 , A61P31/04 , C07D503/00 , C12N9/99 , C12P1/06 , C12P17/18 , C12P37/04 , C07D498/04
CPC分类号： C12P17/188 , A61K31/42 , A61K31/43 , C07D503/00
摘要： A new antibacterially active agent has been isolated from Streptomyces clavuligerus. This new compound which is designated clavulanic acid has the formula (I): ##STR1## In addition to being a broad spectrum antibiotic of medium potency, clavulanic acid and its salts and esters have the ability to enhance the effectiveness of .beta.-lactam antibiotics against many .beta.-lactamase producing bacteria.
摘要翻译：已经从Streptomyces clavuligerus中分离出一种新的抗菌活性剂。这种被称为克拉维酸的新化合物具有式（I）：（I）除了是广谱抗生素的中等效力之外，克拉维酸及其盐和酯还具有增强β - 针对许多β-内酰胺酶生产细菌的内酰胺抗生素。

29. 发明授权

US4250258A Use of phenoxyalkanes as precursors in penicillin V fermentation 失效
标题翻译：在青霉素V发酵中使用苯氧基烷烃作为前体
公开(公告)号：US4250258A
公开(公告)日：1981-02-10
申请号：US105801
申请日：1979-12-20
申请人： Laszlo J. Szarka , Robert W. Eltz
发明人： Laszlo J. Szarka , Robert W. Eltz
IPC分类号： C12P37/00 , C12P37/04
CPC分类号： C12P37/00 , Y10S435/935
摘要： Penicillin V is prepared by culturing a penicillin-producing microorganism in a medium containing suitable sources of nitrogen, carbon and energy, and inorganic salts. One or more phenoxyalkanes of the formula ##STR1## wherein n is an integer from 6 to 13 are included within the medium as the sidechain precursor.
摘要翻译：通过在含有合适的氮，碳和能量来源的介质以及无机盐中培养产青霉素的微生物来制备青霉素V. 作为侧链前体，包含一种或多种式（IMAGE）的苯氧基烷烃，其中n为6至13的整数。

30. 发明授权

US11359224B2 Enzyme-immobilized porous membrane and preparation method of antibiotics using the same 有权
公开(公告)号：US11359224B2
公开(公告)日：2022-06-14
申请号：US16658705
申请日：2019-10-21
申请人： GWANGJU INSTITUTE OF SCIENCE AND TECHNOLOGY
发明人： Ji-woong Park , Sunoh Shin
IPC分类号： C12P37/04 , C12N9/84 , C12N11/093
摘要： The present disclosure relates to an enzyme-immobilized porous membrane and a preparation method of antibiotics using the same, and more specifically, to an enzyme-immobilized porous membrane prepared by immobilizing a specific enzyme through dead-end filtration, and a preparation method of antibiotics with a high yield using the enzyme-immobilized porous membrane.
According to various exemplary embodiments of the present disclosure, the enzyme capable of promoting the synthesis reaction of the antibiotic substance is able to be stably immobilized in the porous membrane by passing the solution of enzyme through the membrane.
In addition, it is possible to provide antibiotics with a high yield by preparing the antibiotics by passing the reactant solution through the enzyme-immobilized porous membrane.

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