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21. 发明申请

US20090111128A1 METHOD FOR PREDICTING IMMUNE RESPONSE TO NEOPLASTIC DISEASE BASED ON mRNA EXPRESSION PROFILE IN NEOPLASTIC CELLS AND STIMULATED LEUKOCYTES 有权
标题翻译：基于神经细胞和刺激白血病细胞中mRNA表达谱的免疫应答预防神经病变的方法
公开(公告)号：US20090111128A1
公开(公告)日：2009-04-30
申请号：US11917151
申请日：2006-06-08
申请人： Masato Mitsuhashi
发明人： Masato Mitsuhashi
IPC分类号： G01N33/53
CPC分类号： G01N33/505 , C12Q1/6806 , C12Q1/6886 , C12Q2600/118 , C12Q2600/158 , G01N33/574
摘要： Tumor necrosis factor (TNF) is capable of inducing apoptosis by interacting with specific TNF receptors on the surface of cancer cells. Because multiple members of TNF ligand and receptor are present within each superfamily, over 300 different ligand-receptor combinations exist. Activated blood leukocytes produce TNF as part of the immune response to cancer, as well as producing chemokines to attract other leukocytes to the site. A method is disclosed of detecting significant induction of a variety of TNF superfamily subtype and chemokine mRNAs in blood leukocytes when whole blood is exposed to heat-aggregated IgG or anti-T cell receptor antibodies as a model of immune system interactions. Substantial individual-to-individual variation is observed in TNF subtypes and chemokines induced. Since peripheral blood leukocytes are the supply of anti-cancer immune cells, the quantitation of ex vivo inducibility of appropriate TNF ligands and chemokines in blood will be useful in individualized cancer immunotherapy. If the tumor mass is small, such as with early invisible metastatic lesions, appropriate TNF assaults may be sufficient to prevent relapse.
摘要翻译：肿瘤坏死因子（TNF）能够通过与癌细胞表面的特异性TNF受体相互作用诱导细胞凋亡。由于TNF配体和受体的多个成员存在于每个超家族中，所以存在超过300种不同的配体 - 受体组合。活化的血液白细胞产生TNF作为对癌症的免疫应答的一部分，以及产生趋化因子以吸引其他白细胞到该部位。公开了当全血暴露于作为免疫系统相互作用的模型的热聚集的IgG或抗T细胞受体抗体时，检测血白细胞中各种TNF超家族亚型和趋化因子mRNA的显着诱导的方法。在TNF亚型和趋化因子诱导中观察到显着的个体与个体差异。由于外周血白细胞是抗癌免疫细胞的供应，所以在个体化的癌症免疫治疗中定量适当的TNF配体和趋化因子在血液中的离体诱导性将是有用的。如果肿瘤质量小，如早期隐形转移性病变，适当的TNF攻击可能足以防止复发。

22. 发明申请

US20080261207A1 Method of Measuring Cancer Susceptibility 审中-公开
标题翻译：测量癌症易感性的方法
公开(公告)号：US20080261207A1
公开(公告)日：2008-10-23
申请号：US11597505
申请日：2005-05-25
申请人： Masato Mitsuhashi
发明人： Masato Mitsuhashi
IPC分类号： C12Q1/68 , C12Q1/02
CPC分类号： C12Q1/6886 , C12Q2600/106 , C12Q2600/136 , C12Q2600/158 , G01N33/5017 , G01N2800/40 , G01N2800/50
摘要： An individual's susceptibility to cancer is assessed based on the individual's cellular response to mutagenic agents such as radiation. The level of a growth-suppressing marker is measured before and after the individual's cells are exposed to the mutagenic agent. The individual's susceptibility to cancer as a result of the mutagenic agent is correlated with the degree to which the growth-suppressing marker is induced by exposure to the agent. A method is also disclosed for assessing cancer prophylaxis effects of compounds, such as vitamins or food extracts, in individuals. Cells from an individual are incubated with at least one compound in vitro, or the compound is directly administered to the individual, after which some of the incubated cells, as well as non-incubated cells, are exposed to a mutagenic agent such as ionizing radiation. The level of the growth-suppressing marker in the cells incubated with the compound and exposed to the mutagenic agent is then compared with the level in the non-incubated cells exposed to the agent. The cancer prophylaxis effects of the compound are correlated with a higher level of the marker in the incubated cells.
摘要翻译：个体对癌症的敏感性是根据个体对诱变剂如辐射的细胞反应进行评估的。在个体细胞暴露于诱变剂之前和之后测量生长抑制标记的水平。致敏物质对个体的癌症易感性与通过暴露于药剂诱导生长抑制标记的程度相关。还公开了一种用于评估化合物（例如维生素或食物提取物）在个体中的癌症预防效果的方法。来自个体的细胞在体外与至少一种化合物一起温育，或者将化合物直接施用于个体，之后将一些培养的细胞以及未孵育的细胞暴露于诱变剂如电离辐射。然后将与化合物孵育并暴露于诱变剂的细胞中的生长抑制标记物的水平与暴露于该试剂的未孵育细胞中的水平进行比较。该化合物的癌症预防作用与培养细胞中较高水平的标记物相关。

23. 发明申请

US20070224631A1 Device and method for high-throughput quantification of mRNA from whole blood 有权
公开(公告)号：US20070224631A1
公开(公告)日：2007-09-27
申请号：US11803593
申请日：2007-05-15
申请人： Masato Mitsuhashi
发明人： Masato Mitsuhashi
IPC分类号： C12Q1/68
CPC分类号： C12N15/1006 , C12N1/066 , C12Q1/6806 , C12Q1/6846 , C12Q2565/537 , C12Q2521/107 , C12Q2527/137 , C12Q2527/125
摘要： Disclosed are a method, device kit, and automated system for simple, reproducible, and high-throughput quantification of mRNA from whole blood. More particularly, the method, device, kit and automated system involve combinations of leukocyte filters attached to oligo(dT)-immobilized multi-well plates.

24. 发明授权

US07237053B1 Clock switching circuit for a hot plug 有权
标题翻译：时钟切换电路用于热插拔
公开(公告)号：US07237053B1
公开(公告)日：2007-06-26
申请号：US09713024
申请日：2000-11-16
申请人： Masato Mitsuhashi , Yoshiyuki Shirai
发明人： Masato Mitsuhashi , Yoshiyuki Shirai
IPC分类号： G06F13/00
CPC分类号： G06F13/4081
摘要： One aspect of the present invention is a clock switching circuit for switching between asynchronous first clock and second clock when connecting or disconnecting an interface cable having a hot-plug function. The clock switching circuit includes a first group of flip-flops for receiving an interface disconnection signal that corresponds to disconnection and connection of the interface cable in response to the first clock, and a second group of flip-flops for receiving the interface disconnection signal in response to the second clock.
摘要翻译：本发明的一个方面是用于在连接或断开具有热插拔功能的接口电缆时，在异步第一时钟和第二时钟之间切换的时钟切换电路。时钟切换电路包括：第一组触发器，用于接收对应于响应于第一时钟的接口电缆的断开和连接的接口断开信号;以及第二组触发器，用于接收接口断开信号响应第二个时钟。

25. 发明申请

US20070101756A1 Air-refrigerant cooling apparatus 审中-公开
标题翻译：空气制冷剂冷却装置
公开(公告)号：US20070101756A1
公开(公告)日：2007-05-10
申请号：US10538177
申请日：2005-06-02
申请人： Seiichi Okuda , Masato Mitsuhashi
发明人： Seiichi Okuda , Masato Mitsuhashi
IPC分类号： F25D21/00 , F25D9/00
CPC分类号： F25B9/004
摘要： The present invention provides a technique for rapidly removing frost accumulated in air-refrigerant cooling apparatuses. An air-refrigerant cooling apparatus in accordance with the present invention includes a bypass line for directly supplying a defroster with refrigerant air having an increased temperature from a compressor, avoiding temperature drop by a heat exchanger and an expansion turbine. Moisture of the frost melted in the defroster is externally exhausted by a fan.
摘要翻译：本发明提供一种快速除去空气 - 制冷剂冷却装置中累积的结霜的技术。根据本发明的空气制冷剂冷却装置包括用于直接向除霜器供给来自压缩机的具有升高的温度的制冷剂空气的旁路管线，以避免热交换器和膨胀涡轮机的温度下降。在除霜器中融化的霜的水分由风扇外部耗尽。

26. 发明申请

US20060275783A1 Gene markers for lung cancer 审中-公开
公开(公告)号：US20060275783A1
公开(公告)日：2006-12-07
申请号：US11255001
申请日：2005-10-19
申请人： Masato Mitsuhashi , Hiroko Matsunaga , Hideki Kambara , Masafumi Kawamura
发明人： Masato Mitsuhashi , Hiroko Matsunaga , Hideki Kambara , Masafumi Kawamura
IPC分类号： C12Q1/68 , A61K39/395 , G01N33/574
CPC分类号： C07H21/04 , C07K16/30 , C12Q1/6809 , C12Q1/686 , C12Q1/6886 , C12Q2600/158 , G01N33/57423 , G01N33/57484 , C12Q2539/113 , C12Q2537/162 , C12Q2525/191 , C12Q2525/15
摘要： A method for the diagnosis and identification of new or residual lung cancer is disclosed which uses newly identified markers for lung cancer including syndecan 1, collagen 1 alpha 2, and two novel proteins, 7013 and 7018. The method involves identification of the lung cancer markers is blood from a patient. It is envisioned that at least one marker may be used or any mixture of the four. The method may also include the identification of cytokeratin-19.

27. 发明授权

US5656462A Method for synthesizing cDNA using a polynucleotide immobilized support 失效
标题翻译：使用多核苷酸固定化载体合成cDNA的方法
公开(公告)号：US5656462A
公开(公告)日：1997-08-12
申请号：US287075
申请日：1994-08-08
申请人： Cylia Keller , Masato Mitsuhashi , Tatsuo Akitaya
发明人： Cylia Keller , Masato Mitsuhashi , Tatsuo Akitaya
IPC分类号： C12N15/09 , C07H21/04 , C12N15/10 , C12P19/34 , C12Q1/68 , G01N33/53 , G01N37/00 , C12N15/00
CPC分类号： C12Q1/6834 , C12N15/1006 , C12N15/1096 , C12P19/34 , C12Q1/6846
摘要： The present invention provides a nucleotide-immobilized support which includes an insoluble support and a polynucleotide bound to the support. This polynucleotide includes at least one sequence complementary to a polyadenylic acid tail of a mRNA. The nucleotide-immobilized support is useful for a variety of methods, including the synthesis of both sense and antisense cDNA, as well as double stranded cDNA. The nucleotide-immobilized support is also useful for synthesis of both sense and antisense mRNA. Additionally, the nucleotide-immobilized support also provides an improved method of placing a cDNA sequence into a vector in a particular orientation. The present invention also includes a new method for binding polynucleotides to insoluble supports.
摘要翻译：本发明提供了一种核苷酸固定的载体，其包含不溶性载体和与载体结合的多核苷酸。该多核苷酸包含与mRNA的聚腺苷酸尾部互补的至少一个序列。核苷酸固定的支持物可用于多种方法，包括有义和反义cDNA的合成以及双链cDNA。核苷酸固定的载体也可用于合成正义和反义mRNA。此外，固定化核苷酸的载体还提供了将cDNA序列置于特定方向的载体中的改进方法。本发明还包括将多核苷酸结合成不溶性载体的新方法。

28. 发明授权

US09758826B2 PUMA, a pro-apoptotic gene, as a novel molecular biomarker for TNFα-induced human islet damage 有权
公开(公告)号：US09758826B2
公开(公告)日：2017-09-12
申请号：US13163326
申请日：2011-06-17
申请人： Yoko Mullen , Masato Mitsuhashi , Keiko Omori
发明人： Yoko Mullen , Masato Mitsuhashi , Keiko Omori
IPC分类号： C12Q1/68 , G01N33/50
CPC分类号： C12Q1/6883 , C12Q2600/136 , C12Q2600/158 , G01N33/507 , G01N2333/4748 , G01N2333/525
摘要： p53-upregulated modulator of apoptosis (PUMA) is a biomarker associated with islet cell health. If PUMA is low, islet cells are typically healthy. If PUMA is high, islet cells are typically unhealthy or dying. PUMA may be measured by either measuring its nucleic or amino acid. PUMA mRNA may be induced by TNF-α stimulation in a time- and dose-dependent manner and β cell apoptosis is induced through a mitochondrial pathway. TNF-α significantly inhibited glucose-induced preproinsulin precursor mRNA synthesis. Such β cell stress signaling in human islets indicates overall state of islet health and, ultimately, the risk of onset and/or degree of severity of both type 1 and type 2 diabetes mellitus.

29. 发明授权

US09719129B2 Methods for isolating vesicles from biological fluids 有权
公开(公告)号：US09719129B2
公开(公告)日：2017-08-01
申请号：US14122920
申请日：2011-06-10
申请人： Masato Mitsuhashi
发明人： Masato Mitsuhashi
IPC分类号： B01D39/20 , C12Q1/68 , C12N15/10
CPC分类号： C12Q1/6806 , B01D39/2017 , C12N15/1003
摘要： Provided is a device that collects vesicles and vesicle-like materials from biological fluids. Such devices comprise at least one sample loading region; at least one corresponding vesicle-capture material, wherein said vesicle-capture material comprises glass-like materials; and at least one corresponding sample receiving region, wherein passage of the biological fluid from the sample loading region through the vesicle capture material and into the sample receiving region results in the capture of vesicles. Additional methods provide for a method of isolating vesicles and vesicle-like materials from biological fluids are also provided.

30. 发明申请

US20130084577A1 METHODS OF CHARACTERIZING HOST RESPONSIVENESS TO INTERFERON BY EX VIVO INDUCTION OF INTERFERON-RESPONSIVE MARKERS 有权
标题翻译：通过EXVIVO诱导干扰素标记表征干扰素对宿主的反应的方法
公开(公告)号：US20130084577A1
公开(公告)日：2013-04-04
申请号：US13695755
申请日：2011-05-03
申请人： Masato Mitsuhashi , Ernest C. Borden
发明人： Masato Mitsuhashi , Ernest C. Borden
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6883 , C12Q1/6886 , C12Q2600/106 , C12Q2600/158
摘要： Embodiments of the invention relate generally to ex vivo methods of quantifying expression of interferon responsive genes and characterizing an individual's potential responsiveness to interferon administration. Certain embodiments relate to methods to monitor the efficacy of ongoing interferon therapy by evaluating expression of interferon responsive genes before and after interferon administration.
摘要翻译：本发明的实施方案一般涉及定量干扰素应答基因的表达的离体方法，并表征个体对干扰素施用的潜在反应性。某些实施方案涉及通过评估干扰素施用前后的干扰素应答基因的表达来监测正在进行的干扰素疗法的功效的方法。

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