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    • 21. 发明授权
    • Synthetic polymer particles for use as standards and calibrators in flow
cytometry
    • 合成聚合物颗粒,用作流式细胞仪中的标准品和校准品
    • US6074879A
    • 2000-06-13
    • US099233
    • 1998-06-17
    • David ZelmanovicWilliam MoskalskiRonny DeClercqFrank LouwetEberhard KuckertWolfgang Podszun
    • David ZelmanovicWilliam MoskalskiRonny DeClercqFrank LouwetEberhard KuckertWolfgang Podszun
    • G01N33/49G01N15/00G01N15/10G01N15/14G01N31/00
    • G01N15/1012G01N2015/1062G01N2015/1486G01N2015/149Y10T436/10Y10T436/101666
    • The invention describes novel methods for calibrating or standardizing flow cytometry instruments using synthetic polymer particles or beads having physical properties which provide advantages for their use in such instruments. The polymer particles or beads employed in the calibration methods are spherical, have an average particle diameter of from about 1 to about 8 microns, a relatively narrow particle distribution, and a low refractive index, i.e., from about 1.35 to about 1.45. The particles are safe, stable for long time periods and are particularly suitable for employment as hematology analyzer calibration substances. The invention provides an advantageous improvement over the types of standards currently used to calibrate a variety of instruments that rely on light scatter combined with other parameters to identify and measure biological particles or cells, as well as nonbiological particles. In particular, using the calibration/standardization method and polymer particles according to the present invention allows hematology instruments to be calibrated and standardized for subsequent accurate and reliable determinations of red blood cells, reticulocytes, white blood cells and platelets in whole blood samples.
    • 本发明描述了使用具有物理性质的合成聚合物颗粒或珠粒来校准或标准化流式细胞仪器的新方法,其提供了在这些仪器中使用的优点。 在校准方法中使用的聚合物颗粒或珠粒是球形的,平均粒径为约1至约8微米,相对较窄的颗粒分布和低折射率,即约1.35至约1.45。 颗粒安全,长时间稳定,特别适合用作血液分析仪校准物质。 本发明提供了相对于目前用于校准依赖于光散射的各种仪器与其他参数相结合以识别和测量生物颗粒或细胞以及非生物颗粒的标准类型的有利改进。 特别地,使用根据本发明的校准/标准化方法和聚合物颗粒允许血液仪器被校准和标准化,用于随后准确可靠地测定全血样品中的红细胞,网织红细胞,白细胞和血小板。
    • 22. 发明授权
    • Monodisperse fluorine-containing synthetic polymer particles having a defined refractive index for use as standards and calibrators in flow cytometry
    • 具有限定折射率的单分散含氟合成聚合物颗粒用作流式细胞术中的标准物和校准物
    • US06521729B1
    • 2003-02-18
    • US09432905
    • 1999-11-02
    • David ZelmanovicWilliam MoskalskiRonny DeClercqFrank LouwetEberhard KuckertWolfgang Podszun
    • David ZelmanovicWilliam MoskalskiRonny DeClercqFrank LouwetEberhard KuckertWolfgang Podszun
    • C08F12024
    • G01N15/1012G01N2015/1062G01N2015/1486G01N2015/149Y10T436/10Y10T436/101666
    • The invention describes novel methods for calibrating or standardizing flow cytometry instruments using synthetic polymer particles or beads having physical properties which provide advantages for their use in such instruments. The polymer particles or beads employed in the calibration methods are spherical, have an average particle diameter of from about 1 to about 8 microns, a relatively narrow particle distribution, and a low refractive index, i.e., from about 1.35 to about 1.45. The particles are safe, stable for long time periods and are particularly suitable for employment as hematology analyzer calibration substances. The invention provides an advantageous improvement over the types of standards currently used to calibrate a variety of instruments that rely on light scatter combined with other parameters to identify and measure biological particles or cells, as well as nonbiological particles. In particular, using the calibration/standardization method and polymer particles according to the present invention allows hematology instruments to be calibrated and standardized for subsequent accurate and reliable determinations of red blood cells, reticulocytes, white blood cells and platelets in whole blood samples.
    • 本发明描述了使用具有物理性质的合成聚合物颗粒或珠粒来校准或标准化流式细胞仪器的新方法,其提供了在这些仪器中使用的优点。 在校准方法中使用的聚合物颗粒或珠粒是球形的,平均粒径为约1至约8微米,相对较窄的颗粒分布和低折射率,即约1.35至约1.45。 颗粒安全,长时间稳定,特别适合用作血液分析仪校准物质。 本发明提供了相对于目前用于校准依赖于光散射的各种仪器与其他参数相结合以识别和测量生物颗粒或细胞以及非生物颗粒的标准类型的有利改进。 特别地,使用根据本发明的校准/标准化方法和聚合物颗粒允许血液仪器被校准和标准化,用于随后准确可靠地测定全血样品中的红细胞,网织红细胞,白细胞和血小板。