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    • 21. 发明授权
    • Method and apparatus for computer controlled cell based diagnosis
    • 用于基于计算机控制的基于细胞的诊断的方法和装置
    • US07346200B1
    • 2008-03-18
    • US10130559
    • 1999-11-18
    • Petros TsipourasTriantafyllos P. Tafas
    • Petros TsipourasTriantafyllos P. Tafas
    • G06K9/00
    • G01N1/312G01N15/1475G01N2001/282G01N2001/317G01N2015/1006G01N2015/1472G01N2015/1477G01N2015/1488G01N2800/36G06K9/00127
    • A computer controlled method for detecting and diagnosing a rare cell type in a tissue sample is provided, said method comprising treating the tissue sample such that it generates a first signal indicative of the presence at a location of a rare cell, detecting the first signal, treating the location at which the first signal is detected to generate a second signal indicative of a diagnostically useful cellular characteristic and detecting the second signal. The first signal can be morphological or a color present in a sought cell either before or after staining. The second signal can be generated by in situ PCR or PCR in situ hybridization. In one preferred embodiment, the rare cell type is fetal cell in a maternal blood tissue sample, said sample consisting of a smear of unenriched maternal blood. In another embodiment, the method is used to diagnose or genotype cancer cells in a blood or tissue biopsy sample.
    • 提供了一种用于检测和诊断组织样本中的稀有细胞类型的计算机控制方法,所述方法包括处理组织样本,使得其产生指示在罕见细胞的位置存在的第一信号,检测第一信号, 处理检测到第一信号的位置以产生指示诊断有用的细胞特征的第二信号并检测第二信号。 在染色之前或之后,第一信号可以是形态学或寻找细胞中存在的颜色。 第二信号可以通过原位PCR或PCR原位杂交产生。 在一个优选实施方案中,稀有细胞类型是母体血液组织样品中的胎儿细胞,所述样品由未培养的母体血液的涂片组成。 在另一个实施方案中,该方法用于诊断或基因型血液或组织活检样本中的癌细胞。