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21. 发明申请

US20070269810A1 Methods and compositions for detecting serotypes of Chlamydia trachomatis capable of causing lymphogranuloma venereum 有权
标题翻译：用于检测能够引起淋巴性肉芽肿的沙眼衣原体血清型的方法和组合物
公开(公告)号：US20070269810A1
公开(公告)日：2007-11-22
申请号：US11436506
申请日：2006-05-18
申请人： Jason Trama , Eli Mordechai , Martin E. Adelson
发明人： Jason Trama , Eli Mordechai , Martin E. Adelson
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689
摘要： Disclosed are methods and compositions for conducting assays of samples utilizing polymerase chain reactions (“PCRs”) in the detection of serotypes of Chlamydia trachomatis capable of causing lymphogranuloma venereum (“LGV”). These serotypes are in the L group of Chlamydia trachomatis, and include the L I, L II, and L III serotypes. These assays take advantage of a deletion occurring in the cytotoxin gene locus specific to the L I, L II, and L III serotypes. The genome of each of these three serotypes contains the nucleotide sequence of SEQ ID NO:1 and the nucleotide sequence of SEQ ID NO:2, wherein the nucleotide at the 3′ end of SEQ ID NO:1 and the nucleotide at the 5′ end of SEQ ID NO:2 are contiguous, and wherein the deletion point of the cytotoxin gene locus is located between these two nucleotides. Each of these assays employs a first primer having a nucleotide sequence flanking one side of the deletion point and a second primer having a nucleotide sequence flanking the other side of the deletion point, wherein the first primer and the second primer are capable of hybridizing respectively to the plus strand and the minus strand of the genome of Chlamydia trachomatis during the PCR. Synthesis during the PCR of a sequence-specific amplicon containing this deletion point indicates that the sample contains nucleic acid specific to an LGV-causing serotype of Chlamydia trachomatis. The amplicon can be detected during real-time PCR using a labeled oligonucleotide as a probe, or after end-point PCR by gel electrophoresis.
摘要翻译：公开了用于在检测能引起淋巴肉芽肿（“LGV”）的沙眼衣原体血清型中使用聚合酶链反应（“PCR”）进行测定的方法和组合物。这些血清型是L组沙眼衣原体，包括L I，L II和L III血清型。这些测定利用在L I，L II和L III血清型特异性的细胞毒素基因位点中发生的缺失。这三种血清型中的每一种的基因组含有SEQ ID NO：1的核苷酸序列和SEQ ID NO：2的核苷酸序列，其中SEQ ID NO：1的3'末端的核苷酸和5' SEQ ID NO：2的末端是连续的，并且其中细胞毒素基因位点的缺失点位于这两个核苷酸之间。这些测定中的每一个采用具有位于缺失点的一侧侧翼的核苷酸序列的第一引物和具有位于缺失点另一侧侧翼的核苷酸序列的第二引物，其中第一引物和第二引物能够分别与 PCR期间沙眼衣原体基因组的正链和负链。在含有该缺失点的序列特异性扩增子的PCR期间的合成表明样品含有对造成衣原体沙眼衣原体的导致LGV的血清型特异的核酸。使用标记的寡核苷酸作为探针，或通过凝胶电泳进行终点PCR后，可以在实时PCR中检测扩增子。

22. 发明授权

US08501408B2 Methods and compositions for detecting and identifying species of Candida 有权
标题翻译：用于检测和鉴定假丝酵母菌种的方法和组合物
公开(公告)号：US08501408B2
公开(公告)日：2013-08-06
申请号：US11321984
申请日：2005-12-29
申请人： Jason Trama , Martin E. Adelson , Eli Mordechai
发明人： Jason Trama , Martin E. Adelson , Eli Mordechai
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6895 , C12Q1/689
摘要： Methods and compositions useful in the detection and identification of species of Candida are disclosed. The compositions are combinations of oligonucleotides, where the forward primers of the primer pairs have identical sequences, while each reverse primer of the primer pairs has a unique sequence relative to all of the other reverse primers; or the reverse primers of the primer pairs have identical sequences, while each forward primer of the primer pairs has a unique sequence relative to all of the other forward primers. The oligonucleotides also include probes capable of detecting these amplicons, and sequencing primers for determining, in primer extension reactions, the nucleotide sequences contained within the amplicons. The detection of an amplicon indicates that the sample contains at least one isolate of Candida albicans, Candida glabrata, Candida parapsilosis, or Candida tropicalis, and the nucleotide sequence data is used to determine which of these four Candida species is present.
摘要翻译：公开了用于检测和鉴定假丝酵母菌种的方法和组合物。组合物是寡核苷酸的组合，其中引物对的正向引物具有相同的序列，而引物对的每个反向引物相对于所有其它反向引物具有唯一的序列; 或引物对的反向引物具有相同的序列，而引物对的每个正向引物相对于所有其它正向引物具有唯一的序列。寡核苷酸还包括能够检测这些扩增子的探针和测序引物，用于在引物延伸反应中测定扩增子中包含的核苷酸序列。扩增子的检测表明该样品含有至少一种白色念珠菌，光滑假丝酵母（Candida glabrata），假丝酵母（Candida parapsilosis）或热带假丝酵母（Candida tropicalis））的分离物，并且使用核苷酸序列数据来确定这四种假丝酵母属中存在哪一种。

23. 发明授权

US08323907B2 Type IV secretion system proteins in sero-detection of Anaplasma phagocytophilum 有权
标题翻译： IV型分泌系统蛋白在血清反应中检测Anaplasma phagocytophilum
公开(公告)号：US08323907B2
公开(公告)日：2012-12-04
申请号：US12658268
申请日：2010-02-09
申请人： John G. Hoey , Denise P. Dimitrov , Lisa P. Huang , Martin E. Adelson , Eli Mordechai
发明人： John G. Hoey , Denise P. Dimitrov , Lisa P. Huang , Martin E. Adelson , Eli Mordechai
IPC分类号： G01N33/53
CPC分类号： C07K14/195 , G01N33/6854 , G01N2469/20
摘要： Disclosed are two (2) proteins in the Type IV Secretion System (TIVSS) in Anaplasma phagocytophilum (namely, virB10 and virB11) useful in the ELISA detection of Anaplasma pathogen. The recombinant expression of virB10 and virB11 and their use as kits for ELISA are also disclosed.
摘要翻译：披露了用于Anaplasma病原体ELISA检测的Anaplasma phagocytophilum（即，virB10和virB11）中的IV型分泌系统（TIVSS）中的两种（2）蛋白质。还公开了virB10和virB11的重组表达及其作为ELISA试剂盒的用途。

24. 发明授权

US07745595B2 Compositions and methods for detecting Atopobium vaginae 有权
标题翻译：组合物和检测阴道的方法
公开(公告)号：US07745595B2
公开(公告)日：2010-06-29
申请号：US11502694
申请日：2006-08-10
申请人： Jason Trama , Martin E. Adelson , Eli Mordechai
发明人： Jason Trama , Martin E. Adelson , Eli Mordechai
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12Q1/689
摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains Atopobium vaginae or has an increased likelihood of containing Atopobium vaginae, an organism which is seen in conjunction with bacterial vaginosis or is a causative agent of bacterial vaginosis. These oligonucleotides, which have nucleotide sequences derived from a segment of the genome of Atopobium vaginae, are useful as forward and reverse primers for a polymerase chain reaction using nucleic acids from a biological sample as a template, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains Atopobium vaginae or has an increased likelihood of containing Atopobium vaginae.
摘要翻译：公开了用于确定样品是否含有阴道弧菌的方法中的寡核苷酸或具有增加的可能性，其含有与细菌性阴道炎联合观察的生物体或阴道细菌性阴道炎的致病因子的阴道角膜的可能性增加。这些寡核苷酸具有源自阴道角膜的基因组的片段的核苷酸序列，可用作使用来自生物样品的核酸作为模板的聚合酶链反应的正向和反向引物，以及用于检测任何所得扩增子的探针。扩增子的检测表明样品含有阴道角膜，或具有增加阴道角化的可能性。

25. 发明申请

US20080213749A1 Compositions and methods for detecting human metapneumovirus 审中-公开
标题翻译：用于检测人类偏肺病毒的组合物和方法
公开(公告)号：US20080213749A1
公开(公告)日：2008-09-04
申请号：US11713408
申请日：2007-03-02
申请人： Melanie Feola , Larisa Gofman , Eli Mordechai , Martin E. Adelson
发明人： Melanie Feola , Larisa Gofman , Eli Mordechai , Martin E. Adelson
IPC分类号： C07H21/00 , C12Q1/68 , C12Q1/70
CPC分类号： C12Q1/701
摘要： Disclosed are oligonucleotides useful in methods for determining whether a sample contains a human metapneumovirus or has an increased likelihood of containing a human metapneumovirus, a virus which is a causative agent of respiratory tract disease in humans. These oligonucleotides, which have nucleotide sequences derived from a coding segment of the gene encoding the fusion protein of a human metapneumovirus, are useful as forward and reverse primers for a polymerase chain reaction using reverse transcripts of RNA from a biological sample as templates, and as probes for detecting any resultant amplicon. Detection of an amplicon indicates the sample contains a human metapneumovirus or has an increased likelihood of containing a human metapneumovirus.
摘要翻译：公开了用于确定样品是否含有人类偏肺病毒或具有增加的可能性的可能性的寡核苷酸，所述可能性包含人类偏肺病毒，人类偏肺病毒是人类呼吸道疾病的致病因子的病毒。具有源自编码人类偏肺病毒融合蛋白的基因的编码区的核苷酸序列的这些寡核苷酸可用作使用来自生物样品的RNA的逆转录作为模板的聚合酶链反应的正向引物和反向引物，以及用于检测任何所得扩增子的探针。扩增子的检测表明样品含有人类偏肺病毒，或具有增加含有人间质肺病毒的可能性。

26. 发明申请

US20130177941A1 BABESIA MICROTI GENOMIC CLONES CONTAINING NOVEL ANTIGENS USEFUL IN THE DIAGNOSIS OF BABESIOSIS 审中-公开
标题翻译：含有新生儿抗生素的BABESIA MICROTI GENOMIC CLONES用于诊断尿崩症
公开(公告)号：US20130177941A1
公开(公告)日：2013-07-11
申请号：US13713155
申请日：2012-12-13
申请人： John G. Hoey , Hannah Venit , Martin E. Adelson , Eli Mordechai , Fernando Valois-Cruz
发明人： John G. Hoey , Hannah Venit , Martin E. Adelson , Eli Mordechai , Fernando Valois-Cruz
IPC分类号： C07K14/44
CPC分类号： C07K14/44 , G01N33/56905
摘要： Disclosed are the cloning and expression of novel antigens in Babesia microti. The recombinant polypeptides are highly immunogenic. The polypeptides of the present invention provide the basis of a diagnostic assay that is sensitive, rapid and accurate using patient's sera. Also disclosed is an IgG and IgM ELISA using two novel recombinant antigens in the diagnosis of Babesia infection.
摘要翻译：公开了在巴贝虫微生物中克隆和表达新型抗原。重组多肽是高度免疫原性的。本发明的多肽提供使用患者血清敏感，快速和准确的诊断测定的基础。还公开了在诊断巴贝虫感染中使用两种新型重组抗原的IgG和IgM ELISA。

27. 发明申请

US20130040323A1 DETECTION KIT CONTAINING A NOVEL RECOMBINANT 15-kDA POLYPEPTIDE USEFUL FOR DETECTING HUMAN INFECTION WITH BARTONELLA HENSELAE 有权
标题翻译：检测包含新型重组体15-kDA多肽用于检测人体感染BARTONELLA HENSELAE
公开(公告)号：US20130040323A1
公开(公告)日：2013-02-14
申请号：US13588134
申请日：2012-08-17
申请人： John G. Hoey , Lisa P. Huang , Martin E. Adelson , Eli Mordechai
发明人： John G. Hoey , Lisa P. Huang , Martin E. Adelson , Eli Mordechai
IPC分类号： G01N33/566
CPC分类号： C07K14/195 , G01N33/56911 , G01N33/6893 , G01N2469/20
摘要： Disclosed are the cloning and expression of a novel antigen of Bartonella henselae. The recombinant polypeptide is found to be highly immunogenic and is useful as a diagnostic test antigen. The polypeptide of the present invention provides the basis of a diagnostic assay that is sensitive, rapid and accurate diagnosis of infection with Bartonella henselae using patient's sera. Disclosed also are the ELISA for both IgG and IgM and allows diagnosis of early and late infection.
摘要翻译：披露了巴氏杆菌的新型抗原的克隆和表达。发现重组多肽是高度免疫原性的，并且可用作诊断测试抗原。本发明的多肽提供了使用患者血清敏感，快速和准确诊断感染巴氏杆菌的诊断测定的基础。还公开了IgG和IgM的ELISA，并且可以诊断早期和晚期感染。

28. 发明授权

US08268569B2 Recombinant 15-kDa polypeptide and use of same in detecting human infection with Bartonella henselae 有权
标题翻译：重组15-kDa多肽，并用于检测人体感染巴氏杆菌
公开(公告)号：US08268569B2
公开(公告)日：2012-09-18
申请号：US12462647
申请日：2009-08-06
申请人： John G. Hoey , Lisa P. Huang , Martin E. Adelson , Eli Mordechai
发明人： John G. Hoey , Lisa P. Huang , Martin E. Adelson , Eli Mordechai
IPC分类号： G01N33/53
CPC分类号： C07K14/195 , G01N33/56911 , G01N33/6893 , G01N2469/20
摘要： Disclosed are the cloning and expression of a novel antigen of Bartonella henselae. The recombinant polypeptide is found to be highly immunogenic and is useful as a diagnostic test antigen. The polypeptide of the present invention provides the basis of a diagnostic assay that is sensitive, rapid and accurate diagnosis of infection with Bartonella henselae using patient's sera. Disclosed also are the ELISA for both IgG and IgM and allows diagnosis of early and late infection.
摘要翻译：披露了巴氏杆菌的新型抗原的克隆和表达。发现重组多肽是高度免疫原性的，并且可用作诊断测试抗原。本发明的多肽提供了使用患者血清敏感，快速和准确诊断感染巴氏杆菌的诊断测定的基础。还公开了IgG和IgM的ELISA，并且可以诊断早期和晚期感染。

29. 发明申请

US20120202227A1 Babesia microti Genomic Clones Containing Novel Antigens Useful in the Diagnosis of Babesiosis 有权
标题翻译： Babesia microti含有新型抗原的基因组克隆可用于诊断巴西菌病
公开(公告)号：US20120202227A1
公开(公告)日：2012-08-09
申请号：US13440106
申请日：2012-04-05
申请人： John G. Hoey , Hannah Venit , Martin E. Adelson , Eli Mordechai , Fernando Valois-Cruz
发明人： John G. Hoey , Hannah Venit , Martin E. Adelson , Eli Mordechai , Fernando Valois-Cruz
IPC分类号： G01N33/53 , C12P21/02 , C07K14/44
CPC分类号： C07K14/44 , G01N33/56905
摘要： Disclosed are the cloning and expression of novel antigens in Babesia microti. The recombinant polypeptides are highly immunogenic. The polypeptides of the present invention provide the basis of a diagnostic assay that is sensitive, rapid and accurate using patient's sera. Also disclosed is an IgG and IgM ELISA using two novel recombinant antigens in the diagnosis of Babesia infection.
摘要翻译：公开了在巴贝虫微生物中克隆和表达新型抗原。重组多肽是高度免疫原性的。本发明的多肽提供使用患者血清敏感，快速和准确的诊断测定的基础。还公开了在诊断巴贝虫感染中使用两种新型重组抗原的IgG和IgM ELISA。

30. 发明授权

US08178310B2 Babesia microti genomic clones containing novel antigens useful in the diagnosis of Babesios 有权
标题翻译：含有可用于诊断巴西菌病的新型抗原的巴贝氏菌微基因组克隆
公开(公告)号：US08178310B2
公开(公告)日：2012-05-15
申请号：US12386097
申请日：2009-04-14
申请人： John G. Hoey , Hannah Venit , Martin E. Adelson , Eli Mordechai , Fernando Valois-Cruz
发明人： John G. Hoey , Hannah Venit , Martin E. Adelson , Eli Mordechai , Fernando Valois-Cruz
IPC分类号： G01N33/569 , C07H21/04 , C07K14/00 , C12P21/06
CPC分类号： C07K14/44 , G01N33/56905
摘要： Disclosed are the cloning and expression of novel antigens in Babesia microti. The recombinant polypeptides are highly immunogenic. The polypeptides of the present invention provide the basis of a diagnostic assay that is sensitive, rapid and accurate using patient's sera. Also disclosed is an IgG and IgM ELISA using two novel recombinant antigens in the diagnosis of Babesia infection.
摘要翻译：公开了在巴贝虫微生物中克隆和表达新型抗原。重组多肽是高度免疫原性的。本发明的多肽提供使用患者血清敏感，快速和准确的诊断测定的基础。还公开了在诊断巴贝虫感染中使用两种新型重组抗原的IgG和IgM ELISA。

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