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    • 28. 发明授权
    • Paramyxoviruses comprising modified transcription start sequence
    • 副粘病毒包括修饰的转录起始序列
    • US07144579B2
    • 2006-12-05
    • US09979908
    • 2001-11-28
    • Yoshiyuki NagaiAtsushi KatoMamoru HasegawaMakoto Inoue
    • Yoshiyuki NagaiAtsushi KatoMamoru HasegawaMakoto Inoue
    • C07H19/00C12N15/63C12N15/66A61K39/12A61K39/155
    • C07K14/005A61K48/00C12N15/86C12N2760/18822C12N2760/18843
    • The present invention provides virus vectors of the family Paramyxoviridae in which the transcription start (S) sequence has been modified so as to modify the expression of genes located downstream thereof, a method for producing the vectors, and uses thereof. By measuring the transcription initiation efficiency of the S sequence of each gene carried by Sendai viruses (SeV), it was clarified that the S sequence of F gene has a significantly lower ability to promote transcription than the other three S sequences. When the S sequence of the F gene of wild type Sendai virus was substituted by the S sequence of the P/M/HN gene-type showing a high transcription initiation efficiency, the F gene of the resultant Sendai virus mutant and genes located downstream thereof show elevated expression levels. It was also revealed that this mutant proliferates more quickly than the wild type. The vectors of this invention are useful in elevating the expression of foreign genes and producing pharmaceutical compositions and vaccines. Furthermore, by lowering virus gene expression from virus vectors, it is possible to suppress transcription and/or replication and reduce cytotoxicity of the vector genome.
    • 本发明提供了副粘病毒科的病毒载体,其中已经修饰了转录起始(S)序列,以便修饰其下游的基因的表达,生成载体的方法及其用途。 通过测量仙台病毒(SeV)携带的每个基因的S序列的转录起始效率,阐明了F基因的S序列比其他三个S序列具有显着较低的促进转录能力。 当野生型仙台病毒的F基因的S序列被显示高转录起始效率的P / M / HN基因型的S序列取代时,得到的仙台病毒突变体的F基因和位于其下游的基因 显示升高的表达水平。 还显示,该突变体比野生型增殖更快。 本发明的载体可用于提高外源基因的表达并产生药物组合物和疫苗。 此外,通过降低来自病毒载体的病毒基因表达,可以抑制转录和/或复制并降低载体基因组的细胞毒性。