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21. 发明授权

US4855225A Method of detecting electrophoretically separated oligonucleotides 失效
标题翻译：检测电泳分离的寡核苷酸的方法
公开(公告)号：US4855225A
公开(公告)日：1989-08-08
申请号：US827348
申请日：1986-02-07
申请人： Steven Fung , Sam L. Woo , Richard P. Haugland , Steven M. Menchen , Charles R. Connell
发明人： Steven Fung , Sam L. Woo , Richard P. Haugland , Steven M. Menchen , Charles R. Connell
IPC分类号： C07H21/04 , C07H1/06 , C07H21/00 , C12Q1/68 , G01N27/26 , G01N27/447 , G01N33/50 , G01N33/58
CPC分类号： G01N33/582 , C07H21/00 , C12Q1/6816 , C12Q1/6874
摘要： A method is provided for detecting up to four classes of oligonucleotides which have been separated by gel electrophoresis. The method entails labeling members of each class of oligonucleotide with dyes selected from separate sets of dyes so that members of the same class are labeled with dyes from the same set. The four sets of dyes of the invention consist of derivatives of fluorescein, 2',7'-dimethoxy-4', 5'-dichlorofluorescein, tetramethylrhodamine, and rhodamine X carboxylic or sulfonic acid, respectively. Dyes from these sets are spectrally resolvable under conditions of gel electrophoresis.
摘要翻译：提供了一种用于检测多达四类通过凝胶电泳分离的寡核苷酸的方法。该方法需要用选自单独的染料组的染料标记每一类寡核苷酸的成员，使得同一类的成员用来自同一组的染料标记。本发明的四组染料分别由荧光素，2'，7'-二甲氧基-4'，5'-二氯荧光素，四甲基罗丹明和罗丹明X羧酸或磺酸的衍生物组成。来自这些组的染料在凝胶电泳条件下是光谱可分辨的。

22. 发明授权

US06743905B2 Mobility-modified nucleobase polymers and methods of using same 有权
标题翻译：流动性修饰的核碱基聚合物及其使用方法
公开(公告)号：US06743905B2
公开(公告)日：2004-06-01
申请号：US09836704
申请日：2001-04-16
申请人： Sam L. Woo , Ron Graham , Jing Tian
发明人： Sam L. Woo , Ron Graham , Jing Tian
IPC分类号： C07H2100
CPC分类号： C12Q1/6813 , C07H21/04 , C12Q2565/137 , C12Q2525/197
摘要： The present invention relates generally to nucleobase polymer functionalizing reagents, to mobility-modified sequence-specific nucleobase polymers, to compositions comprising a plurality of mobility-modified sequence-specific nucleobase polymers, and to the use of such polymers and compositions in a variety of assays, such as, for example, for the detection of a plurality of selected nucleotide sequences within one or more target nucleic acids. The mobility-modifying polymers of the present invention include phosphoramidite reagents which can be joined to other mobility-modifying monomers and to sequence-specific oligonucleobase polymers via uncharged phosphate triester linkages. Addition of the mobility-modifying phosphoramidite reagents of the present invention to oligonucleobase polymers results in unexpectedly large effects the mobility of those modified oligonucleobase polymers, especially upon capillary electrophoresis in non-sieving media.
摘要翻译：本发明一般涉及核碱基聚合物官能化试剂，迁移率修饰的序列特异性核碱基聚合物，包含多种迁移率修饰的序列特异性核碱基聚合物的组合物，以及这些聚合物和组合物在多种测定中的用途，例如用于检测一个或多个靶核酸内的多个选择的核苷酸序列。本发明的迁移率改性聚合物包括亚磷酰胺试剂，其可以通过不带电荷的磷酸三酯键连接到其它迁移率改性单体和序列特异性寡核苷酸聚合物。将本发明的迁移率修饰亚磷酰胺试剂加入到寡核苷酸聚合物中导致这些修饰的寡核苷酸聚合物的迁移率出人意料地大的影响，特别是在非筛选培养基中的毛细管电泳。

23. 发明授权

US4965349A Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports 失效
标题翻译：在固相载体上合成用氨不稳定基团标记的寡核苷酸的方法
公开(公告)号：US4965349A
公开(公告)日：1990-10-23
申请号：US138287
申请日：1987-12-24
申请人： Sam L. Woo , Steven M. Menchen , Steven Fung
发明人： Sam L. Woo , Steven M. Menchen , Steven Fung
IPC分类号： C12N15/09 , C07F9/24 , C07H21/00
CPC分类号： C07H21/00 , C07F9/24
摘要： The invention provides a novel cleavage reagent for hydrolysing base-labile linking groups between a solid phase support and oligonucleotides. The cleavage reagent comprises a lower alkyl alcohol, water, and a non-nuccleophilic hindered alkylamine containing from 3 to 6 carbon atoms in a ratio of about 1:1:1 to about 1:3:1, respectively. An important property of the cleavage reagent is that it preserves the fluorescent characteristics of rhodamine dyes during cleavage, thereby making it possible to completely synthesize rhodamine-labeled oligonucleotides by solid phase methods. Rhodamine phosphoramidites are provided to further enhance the efficiency of synthesizing rhodamine-labeled oligonucleotides by such methods.

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