专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

21. 发明申请

US20150004691A1 Novel shRNA Molecules and Methods of Use Thereof 有权
公开(公告)号：US20150004691A1
公开(公告)日：2015-01-01
申请号：US14318163
申请日：2014-06-27
申请人： Gradalis, Inc.
发明人： Donald Rao
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , A61K48/00 , C07H21/02 , C12N15/111 , C12N15/85 , C12N2310/14 , C12N2310/531 , C12N2320/30 , C12N2320/31
摘要： The present invention relates to certain novel shRNA molecules and methods of use thereof. According to certain embodiments of the present invention, methods for reducing the expression level of a target gene are provided. Such methods generally comprise providing a cell with one or more precursor nucleic acid sequences that encode two or more RNA molecules. A first RNA molecule comprises a double stranded sequence, which includes a guide strand sequence that is complementary to a portion of an mRNA transcript encoded by the target gene. In addition, a second RNA molecule comprises a second double stranded sequence, which includes a second guide strand sequence that is partially complementary to a portion of the mRNA transcript encoded by the target gene. Preferably, the second guide strand sequence comprises one or more bases that are mismatched with a nucleic acid sequence of the mRNA transcript encoded by the target gene.

22. 发明申请

US20130295160A1 ANTAGONISTS OF MIR-196A 审中-公开
标题翻译： MIR-196A的拮抗剂
公开(公告)号：US20130295160A1
公开(公告)日：2013-11-07
申请号：US13738742
申请日：2013-01-10
申请人： Baylor College of Medicine , Gradalis, Inc.
发明人： Donald Rao , Zhaohui Wang , John J. Nemunaitis , Changyi Chen , Qizhi Yao
IPC分类号： C12N15/113 , A61K48/00
CPC分类号： C12N15/1135 , A61K48/005 , C12N15/113 , C12N2310/113 , C12N2310/531 , C12N2320/30 , C12N2320/32 , C12N2330/50 , C12N2330/51
摘要： A miR-196a antagonist capable of inhibiting a miR-196a activity, the miR-196a antagonist comprising one or more target sites for miR-196a. Included is also an expression vector comprising a promoter and a nucleic acid insert operably linked to the promoter, wherein the insert encodes one or more miR-196a antagonists capable of inhibiting a miR-196a activity. In one example, the one or more miR-196a antagonists comprise at least one stem-loop structure comprising a guide strand that comprises a sequence that is complementary to miR-196a, the stem-loop structure further comprising a passenger strand that comprises a mismatch.
摘要翻译：能够抑制miR-196a活性的miR-196a拮抗剂包含miR-196a的一个或多个靶位点的miR-196a拮抗剂。包括的还包括与启动子可操作地连接的启动子和核酸插入物的表达载体，其中插入物编码能够抑制miR-196a活性的一种或多种miR-196a拮抗剂。在一个实例中，所述一种或多种miR-196a拮抗剂包含至少一个包含引导链的茎 - 环结构，所述引导链包含与miR-196a互补的序列，所述茎 - 环结构进一步包括包含错配的乘客链。

23. 发明申请

US20130259927A1 Ewing's Sarcoma Bifunctional shRNA Design 审中-公开
标题翻译：尤因肉瘤双功能shRNA设计
公开(公告)号：US20130259927A1
公开(公告)日：2013-10-03
申请号：US13855109
申请日：2013-04-02
申请人： GRADALIS, INC.
发明人： John J. Nemunaitis , Donald Rao , Neil Senzer
IPC分类号： C12N15/113
CPC分类号： C12N15/1135 , C12N2310/51 , C12N2310/14 , C12N2310/531
摘要： The present invention includes compositions and methods of making and using an imaging label comprising an expression vector comprising a promoter; and a nucleic acid insert operably linked to the promoter, wherein the insert encodes one or more short hairpin RNAs (shRNA) capable of inhibiting an expression of a target gene sequence that is a EWS-FLI1 fusion gene, a EWSR1-ERG fusion gene, or both in Ewing's sarcoma via RNA interference; wherein the one or more shRNA comprise a bifunctional RNA molecule that activates a cleavage-dependent and a cleavage-independent RNA-induced silencing complex for reducing the expression level of the target gene.
摘要翻译：本发明包括制备和使用包含表达载体的成像标记物的组合物和方法，所述表达载体包含启动子; 和与所述启动子可操作地连接的核酸插入物，其中所述插入物编码能够抑制作为EWS-FLI1融合基因的靶基因序列的表达的一种或多种短发夹RNA（shRNA），EWSR1-ERG融合基因，或通过RNA干扰在尤文氏肉瘤中两者; 其中所述一个或多个shRNA包含激活切割依赖性的双功能RNA分子和用于降低靶基因表达水平的切割非依赖性RNA诱导的沉默复合物。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式