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21. 发明授权

US09890421B2 Assay systems for genetic analysis 有权
公开(公告)号：US09890421B2
公开(公告)日：2018-02-13
申请号：US13205570
申请日：2011-08-08
申请人： Andrew Sparks , Arnold Oliphant , Jacob Zahn , Ken Song , John Stuelpnagel
发明人： Andrew Sparks , Arnold Oliphant , Jacob Zahn , Ken Song , John Stuelpnagel
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6809 , C12Q1/6862 , C12Q2525/155 , C12Q2525/161 , C12Q2533/107 , C12Q2535/131 , C12Q2565/514 , C12Q2565/543
摘要： The present invention provides assays systems and methods for detection of chromosomal abnormalities and status of single loci associated with monogenic or polygenic traits in a sample containing nucleic acids from a maternal and a fetal source.

22. 发明申请

US20090111706A1 SELECTION OF DNA ADAPTOR ORIENTATION BY AMPLIFICATION 审中-公开
标题翻译：通过放大选择DNA适配器取向
公开(公告)号：US20090111706A1
公开(公告)日：2009-04-30
申请号：US11934703
申请日：2007-11-02
申请人： Andrew Sparks , Arnold Oliphant , Radoje Drmanac
发明人： Andrew Sparks , Arnold Oliphant , Radoje Drmanac
IPC分类号： C40B30/04 , C12N15/11 , C40B40/08
CPC分类号： C12N15/10 , C12N15/66
摘要： Aspects described and claimed herein provide methods to insert multiple DNA adaptors into a population of circular target DNAs at defined positions and orientations with respect to one another by employing amplification procedures. The resulting multi-adaptor constructs are then used in massively-parallel nucleic acid sequencing techniques.
摘要翻译：本文描述和要求保护的方面提供了通过使用扩增程序将多个DNA衔接子插入相对于彼此的限定位置和取向的环状靶DNA群中的方法。然后将所得多连接体构建体用于大规模并行核酸测序技术。

23. 发明申请

US20090111705A1 SELECTION OF DNA ADAPTOR ORIENTATION BY HYBRID CAPTURE 审中-公开
标题翻译：通过混合捕获选择DNA适配器方向
公开(公告)号：US20090111705A1
公开(公告)日：2009-04-30
申请号：US11934697
申请日：2007-11-02
申请人： Andrew Sparks , Arnold Oliphant , George Yeung
发明人： Andrew Sparks , Arnold Oliphant , George Yeung
IPC分类号： C40B30/04 , C12N15/00 , C40B40/08 , C12N9/00
CPC分类号： C12N15/10 , C12N15/66
摘要： Aspects described and claimed herein provide methods to insert multiple DNA adaptors into a population of circular target DNAs at defined positions and orientations with respect to one another by employing selective capture of defined molecules. The resulting multi-adaptor constructs are then used in massively-parallel nucleic acid sequencing techniques.
摘要翻译：在此描述和要求保护的方面提供了通过采用选择性捕获限定的分子将多个DNA适配器插入到相对于彼此的限定位置和取向的环状靶DNA群中的方法。然后将所得多连接体构建体用于大规模并行核酸测序技术。

24. 发明申请

US20090075343A1 SELECTION OF DNA ADAPTOR ORIENTATION BY NICKING 审中-公开
标题翻译：选择DNA适配器取向
公开(公告)号：US20090075343A1
公开(公告)日：2009-03-19
申请号：US11934695
申请日：2007-11-02
申请人： Andrew Sparks , Steven Huang , Radoje Drmanac , Arnold Oliphant
发明人： Andrew Sparks , Steven Huang , Radoje Drmanac , Arnold Oliphant
IPC分类号： C12P19/34
CPC分类号： C12N15/10 , C12N15/66
摘要： Aspects described and claimed herein provide methods to insert multiple DNA adaptors into a population of circular target DNAs at defined positions and orientations with respect to one another using nicking reactions. The resulting multi-adaptor constructs are then used in massively-parallel nucleic acid sequencing techniques.
摘要翻译：在此描述和要求保护的方面提供了使用切口反应将多个DNA适配器插入到定义的位置和相对于彼此的取向的圆形靶DNA群中的方法。然后将所得多连接体构建体用于大规模并行核酸测序技术。

25. 发明申请

US20100076185A1 Selective Processing of Biological Material on a Microarray Substrate 审中-公开
标题翻译：微阵列基板上生物材料的选择性处理
公开(公告)号：US20100076185A1
公开(公告)日：2010-03-25
申请号：US12562543
申请日：2009-09-18
申请人： Nils Adey , Arnold Oliphant , Wanyuan Ao
发明人： Nils Adey , Arnold Oliphant , Wanyuan Ao
IPC分类号： C07H21/04 , C40B60/12 , C40B50/00
CPC分类号： C12Q1/6837
摘要： The present invention provides methods and systems for selectively eluting biological material from a distinct spatial location on a microarray slide. In one aspect, for example, a method for recovering biological material coupled to a microarray slide can include selecting a biological material to be recovered from the microarray slide, finding the biological material within a distinct spatial region on the microarray slide surface, and eluting at least a portion of the selected biological material from the distinct spatial region without eluting substantial amounts of non-selected biological material from regions of the microarray slide that are not within the distinct spatial region.
摘要翻译：本发明提供了用于从微阵列载玻片上的不同空间位置选择性洗脱生物材料的方法和系统。在一个方面，例如，用于回收与微阵列载玻片耦合的生物材料的方法可以包括从微阵列载玻片上选择要回收的生物材料，在微阵列载玻片表面上的不同空间区域内找到生物材料，并在来自不同空间区域的所选择的生物材料的至少一部分，而不从不在不同空间区域内的微阵列载玻片的区域洗脱大量未选择的生物材料。

26. 发明申请

US20050266432A1 Haplotype markers for diagnosing susceptibility to immunological conditions 审中-公开
标题翻译：用于诊断免疫条件易感性的单倍型标记
公开(公告)号：US20050266432A1
公开(公告)日：2005-12-01
申请号：US11069908
申请日：2005-02-28
申请人： Arnold Oliphant , Sarah Murray
发明人： Arnold Oliphant , Sarah Murray
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12Q2600/156 , C12Q2600/172
摘要： The invention provides a method of identifying a SNP haplotype that correlates with an HLA type, MHC allele or susceptibility to a particular disease or condition including the steps of (a) providing the identity of the nucleotide for each of a set of single nucleotide polymorphism (SNPs) in the major histocompatability complex (MHC) region in a population of individuals; (b) providing the HLA type, MHC allele, or susceptibility to the particular disease or condition for the individuals; and (c) identifying a SNP haplotype in the population that correlates with the HLA type, MHC allele or susceptibility to the particular disease or condition, wherein the SNP haplotype includes the SNPs in the MHC region. The invention further provides a method for predicting an HLA type, MHC allele or susceptibility to a particular disease or condition of an individual including the steps of (a) providing the identity of a plurality of SNPs in one or more nucleic acids from the individual that correlate with an HLA type, MHC allele or susceptibility to a particular disease or condition; and (b) predicting the HLA type, MHC genotype or susceptibility to a particular disease or condition for the individual based on a SNP haplotype comprising the plurality of SNPs. The invention also provides SNPs and oligonucleotide probes that are useful for identifying haplotypes associated with a particular disease or condition or with the presence of particular protein variants involved in immunological recognition. The SNPs and probes are also useful for predicting HLA-type, HLA genotype or susceptibility to a particular disease or condition.
摘要翻译：本发明提供了鉴定与特定疾病或病症的HLA类型，MHC等位基因或易感性相关的SNP单倍型的方法，包括以下步骤：（a）提供一组单核苷酸多态性中的每一个的核苷酸的身份（ SNPs）在个体人群中的主要组织相容性复合体（MHC）区域; （b）为个体提供HLA类型，MHC等位基因或对特定疾病或病症的易感性; 和（c）识别与HLA类型，MHC等位基因或对特定疾病或病症的易感性相关的群体中的SNP单倍型，其中所述SNP单倍型包括MHC区域中的SNP。本发明还提供了一种用于预测HLA类型，MHC等位基因或对个体的特定疾病或病症的易感性的方法，包括以下步骤：（a）在个体的一个或多个核酸中提供多个SNP的身份，与HLA类型，MHC等位基因或对特定疾病或病症的易感性相关; 和（b）基于包含多个SNP的SNP单元型，预测HLA类型，MHC基因型或对个体的特定疾病或病症的易感性。本发明还提供可用于鉴定与特定疾病或病症相关的单倍型或涉及免疫学识别的特定蛋白质变体的存在的SNP和寡核苷酸探针。 SNP和探针也可用于预测HLA型，HLA基因型或对特定疾病或病症的易感性。

27. 发明授权

US06251610B1 Method for sequencing both strands of a double stranded DNA in a single sequencing reaction 失效
标题翻译：在单次测序反应中测序双链DNA两条链的方法
公开(公告)号：US06251610B1
公开(公告)日：2001-06-26
申请号：US09664560
申请日：2000-09-18
申请人： Jamila Gupte , Arnold Oliphant
发明人： Jamila Gupte , Arnold Oliphant
IPC分类号： C12P1934
CPC分类号： C12N15/10 , C12Q1/686 , C12Q1/6869 , C12Q2535/119
摘要： A method is presented which uses a unique opposite strand joining strategy during PCR of an original DNA to generate a product which, when sequenced with a single sequencing primer yields the sequence of both strands of the original DNA. The PCR primers include 1) a modified oligomer corresponding to the 5′ end of a first strand of the DNA to be amplified wherein said modified oligomer includes the reverse complementary sequence to a sequence within said first strand of DNA and a specific PCR priming sequence which will specifically hybridize to a portion of the DNA to be amplified and 2) a second oligomer corresponding to the 5′ end of the second strand of the DNA to be amplified and which contains the priming sequence for the second strand of the DNA and will specifically hybridize to a portion of the DNA to be amplified. During PCR an intermediate product is formed where one end of one strand loops around to hybridize to its complement on the same strand. This results in a hairpin structure which elongates using its own strand as a template to form a double sized product that contains the sequence of both original strands. Upon denaturation this yields single strands with the single strands having the sequence of both of the original strands included in tandem. Sequencing these single strands using a single primer, e.g., a primer complementary to the second oligomer, yields the sequences of both strands of the DNA of interest.
摘要翻译：提出了一种在原始DNA的PCR期间使用独特的相反链连接策略以产生产物的方法，当用单个测序引物测序时，产生原始DNA的两条链的序列。 PCR引物包括1）对应于待扩增的DNA的第一链的5'端的修饰的寡聚体，其中所述修饰的寡聚体包括与所述第一链DNA内的序列的反向互补序列和特异性PCR引物序列，将与要扩增的DNA的一部分特异性杂交，以及2）对应于待扩增的DNA的第2链的5'末端的第二寡聚体，其含有第二链的引物序列，与要扩增的DNA的一部分杂交。在PCR期间，形成中间产物，其中一条链的一端环绕在与相同链上的其互补体杂交。这导致发夹结构，其使用其自身的链作为模板延长以形成包含两条原始链的序列的双重尺寸的产物。变性时，这产生单链，单链具有串联包含的两条原始链的序列。使用单一引物（例如与第二寡聚体互补的引物）测序这些单链，产生目的DNA的两条链的序列。

28. 发明申请

US20130244882A1 MULTIPLEX NUCLEIC ACID REACTIONS 有权
公开(公告)号：US20130244882A1
公开(公告)日：2013-09-19
申请号：US13604872
申请日：2012-09-06
申请人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
发明人： Arnold Oliphant , John R. Stuelpnagel , Mark S. Chee , Scott L. Butler , Jian-Bing Fan , Min-Jui Richard Shen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6834 , C12Q1/6837 , C12Q2600/16 , Y10T436/143333 , C12Q2525/155 , C12Q2563/131 , C12Q2563/179
摘要： A method for detecting nucleic acids by (a) providing a sample having target nucleic acids, each nucleic acid having contiguous first, second, and third domains; (b) contacting the sample with probe sets to form hybridization complexes, wherein each probe set includes (i) a first probe having a sequence that is complementary to the first domain; and (ii) a second probe having a sequence substantially complementary to the third domain; (c) extending the first probes along the second domains of the complexes while the complexes are immobilized on a solid support; (d) ligating the extended first probes to the second probes to form templates; (e) amplifying the templates with primers that are complementary to the first and second priming sequences to produce amplicons; and (f) detecting the amplicons on the surface of a nucleic acid array.

29. 发明申请

US20060213881A1 Laser ablation of doped fluorocarbon materials and applications thereof 审中-公开
标题翻译：掺杂氟碳材料的激光烧蚀及其应用
公开(公告)号：US20060213881A1
公开(公告)日：2006-09-28
申请号：US11445536
申请日：2006-06-02
申请人： Arnold Oliphant , Michael McNeely
发明人： Arnold Oliphant , Michael McNeely
IPC分类号： B23K26/36
CPC分类号： B23K26/18 , B23K26/361 , B23K26/40 , B23K2103/42 , B23K2103/50
摘要： A method for laser ablation of doped fluorocarbon materials, such as fluorocarbon resins, and article fabrication applications using the laser ablation are disclosed. More specifically, a UV absorbing additive, such as carbon black, is compounded with a fluorocarbon resin which is then subjected to laser ablation. The present invention is particularly useful for bulk structure fabrication, for example microstructure micro fabrication.
摘要翻译：公开了一种用于激光烧蚀掺杂碳氟化合物材料的方法，例如氟碳树脂，以及使用激光烧蚀的制品应用。更具体地，诸如炭黑的UV吸收添加剂与氟碳树脂复合，然后进行激光烧蚀。本发明特别适用于本体结构的制造，例如微结构微观制造。

30. 发明申请

US20120219950A1 ASSAY SYSTEMS FOR DETECTION OF ANEUPLOIDY AND SEX DETERMINATION 审中-公开
标题翻译：用于检测异常和性别测定的测定系统
公开(公告)号：US20120219950A1
公开(公告)日：2012-08-30
申请号：US13405839
申请日：2012-02-27
申请人： Arnold Oliphant , Ken Song
发明人： Arnold Oliphant , Ken Song
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6879 , C12Q1/6883 , C12Q2600/156 , C12Q2600/16
摘要： The present invention utilizes detection of selected nucleic acid regions from pseudoautosomal regions to identify sex chromosomal aneuploidy and to determine fetal sex. Traditional methods of detecting sex chromosomal aneuploidies and performing sex determination typically involves some analysis of the Y chromosome. The assay systems of the present invention utilizing copy number variant detection of pseudoautosomal regions allows quantification of the sex chromosomes in mixed samples using loci that display autosomal inheritance patterns.
摘要翻译：本发明利用来自假性染色体区域的选择的核酸区域的检测来鉴定性染色体非整倍性并确定胎儿性别。检测性染色体非整倍体和进行性别决定的传统方法通常涉及Y染色体的一些分析。利用伪染色体区域的拷贝数变体检测的本发明的测定系统允许使用显示常染色体遗传模式的位点对混合样品中的性染色体进行定量。

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