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21. 发明授权

US08070958B2 Separation matrix having a ligand density gradient 失效
标题翻译：具有配体密度梯度的分离基质
公开(公告)号：US08070958B2
公开(公告)日：2011-12-06
申请号：US12692671
申请日：2010-01-25
申请人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Anders Ljunglof , Jean-Luc Maloisel
发明人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Anders Ljunglof , Jean-Luc Maloisel
IPC分类号： B01D15/08 , C02F1/28
CPC分类号： B01J20/3285 , B01D15/364 , B01J20/286 , B01J20/3244
摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.
摘要翻译：本发明涉及由耦合到多孔载体的表面的配体（例如一种或多种多孔颗粒）组成的分离基质，其中配体在载体内提供至少一种化学梯度。在最有利的实施方案中，化学梯度是配体密度梯度。本发明还涉及一种提供包含耦合到多孔载体表面的配体的分离基质的方法，其中至少一种配体密度梯度由至少一种试剂的溶剂控制扩散提供到多孔载体中。

22. 发明申请

US20110065900A1 SEPARATION METHOD UTILIZING POLYALLYLAMINE LIGANDS 审中-公开
标题翻译：利用聚氨酯配体的分离方法
公开(公告)号：US20110065900A1
公开(公告)日：2011-03-17
申请号：US12993152
申请日：2009-05-29
申请人： Bo-Lennart Johansson , Ulrika Karlsson , Jean-Luc Maloisel
发明人： Bo-Lennart Johansson , Ulrika Karlsson , Jean-Luc Maloisel
IPC分类号： C07K16/46 , C07K1/18 , C07K16/00 , B01D15/36
CPC分类号： B01D15/363 , B01J20/3242 , B01J20/327 , B01J41/20 , G01N30/96
摘要： The present invention relates to a method for removing at least one negatively charged substance from an aqueous liquid by contacting the liquid with a separation matrix comprising a plurality of polyallylamine ligands, comprising binding said negatively charged substance to said ligands under conditions where the ionic strength of the aqueous liquid applied to the chromatography resin ≧0.25 M NaCl.
摘要翻译：本发明涉及通过使液体与包含多个聚烯丙基胺配体的分离基质接触从水性液体中除去至少一种带负电荷的物质的方法，包括在所述配体的离子强度水性液体施加到色谱树脂≥0.25MNaCl。

23. 发明申请

US20080299671A1 Hydrophobic Interaction Chromatography 审中-公开
标题翻译：疏水相互作用色谱
公开(公告)号：US20080299671A1
公开(公告)日：2008-12-04
申请号：US12095752
申请日：2006-11-30
申请人： Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman
发明人： Gunnar Glad , Bo-Lennart Johansson , Jean-Luc Maloisel , Nils Norrman
IPC分类号： G01N30/02 , B01D15/16 , B01D15/32 , C07K16/06 , C07K1/00
CPC分类号： C07K16/065 , B01D15/327 , B01J20/262 , B01J20/265 , B01J20/285 , B01J20/286 , B01J20/289
摘要： The present invention relates to a method of isolating target compounds from a liquid, which method including the steps of providing a mobile phase, which contains at least one target compound and wherein the conductivity corresponds to ≧0.6 M; contacting the mobile phase with a separation matrix including one or more sulphonamide groups to adsorb one or more target compounds; contacting an eluent with the matrix to release target compound(s), wherein the conductivity of the eluent is reduced as compared to the mobile phase conductivity and the pH is substantially equivalent to the mobile phase pH; and, optionally, recovering at least one target compound or a purified liquid.
摘要翻译：本发明涉及一种从液体中分离目标化合物的方法，该方法包括以下步骤：提供含有至少一种目标化合物的流动相，其中电导率对应于> = 0.6M; 使流动相与包含一个或多个磺酰胺基团的分离基质接触以吸附一种或多种目标化合物; 使洗脱液与基质接触以释放目标化合物，其中与流动相电导率相比，洗脱液的电导率降低，并且pH基本上等于流动相pH; 和任选地回收至少一种目标化合物或纯化液体。

24. 发明授权

US07320755B2 Method of preparing ligands for hydrophobic interaction chromatography 失效
标题翻译：制备疏水相互作用色谱配体的方法
公开(公告)号：US07320755B2
公开(公告)日：2008-01-22
申请号：US10547570
申请日：2004-03-05
申请人： Bo-Lennart Johansson , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Bo-Lennart Johansson , Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： B01D15/08
CPC分类号： B01J20/3285 , B01D15/325 , B01D15/327 , B01J20/286
摘要： The invention is a method of preparing multimodal ligands for hydrophobic interaction chromatography (HIC), which comprises providing a cyclic scaffold comprising a thiol, an amine and a carbonyl group; derivatisation of the nitrogen with a reagent to introduce a primary interaction; and aminolysis of the resulting derivative, whereby a secondary interaction is introduced next to the carbonyl; wherein at least one of the primary interaction and the secondary interaction comprises a hydrophobic group and wherein non of said interactions comprises charged groups, i.e. ion exchange ligands. The invention also encompasses a separation medium comprising such multimodal ligands immobilised to a base matrix.
摘要翻译：本发明是制备用于疏水相互作用色谱（HIC）的多峰配体的方法，其包括提供包含硫醇，胺和羰基的环状支架; 用试剂衍生氮以引入主要相互作用; 和所得衍生物的氨解，由此在羰基旁边引入二次相互作用; 其中所述主要相互作用和所述第二相互作用中的至少一种包含疏水基团，并且其中所述相互作用中的非相互作用包括带电基团，即离子交换配体。本发明还包括一种分离介质，其包含固定在基础基质上的这种多峰配体。

25. 发明申请

US20070259453A1 Method of Antibody Purification 有权
标题翻译：抗体纯化方法
公开(公告)号：US20070259453A1
公开(公告)日：2007-11-08
申请号：US11577203
申请日：2005-10-21
申请人： Carina Engstrand , Annika Forss , Bo-Lennart Johansson , Hans Johansson , Jean-Luc Maloisel , Gunnar Glad
发明人： Carina Engstrand , Annika Forss , Bo-Lennart Johansson , Hans Johansson , Jean-Luc Maloisel , Gunnar Glad
IPC分类号： G01N33/53
CPC分类号： B01D15/3828 , B01D15/327 , B01D15/363 , B01D15/3804 , B01D15/3809 , B01D15/3847 , B01J20/286 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/165 , C07K1/36 , C07K16/00 , C07K16/065
摘要： The present invention relates to a method of separating antibodies from other compound(s) in a liquid sample, wherein a mobile phase comprising said sample is contacted with a multi-modal separation matrix to adsorb undesired compounds while the antibodies remain free in the liquid, wherein the multi-modal separation matrix comprises first groups, which are capable of interacting with negatively charged sites of the target compounds, and second groups, which are capable of at least one interaction other than charge-charge interaction with said target compounds. The invention also relates to a chromatography column packed with the above-described multi-modal separation matrix and a filter having such multi-modal groups adsorbed to its surface
摘要翻译：本发明涉及从液体样品中的其它化合物分离抗体的方法，其中包含所述样品的流动相与多模式分离基质接触以吸附不需要的化合物，同时抗体在液体中保持游离，其中所述多模式分离基质包含能够与所述目标化合物的带负电荷位点相互作用的第一组和能够与所述目标化合物的电荷 - 电荷相互作用以外的至少一个相互作用的第二组。本发明还涉及一种填充有上述多模式分离基质的色谱柱和一种具有吸附在其表面上的多模式基团的过滤器

26. 发明申请

US20070244307A1 Chromatography Ligand 有权
标题翻译：色谱配体
公开(公告)号：US20070244307A1
公开(公告)日：2007-10-18
申请号：US11577208
申请日：2005-10-21
申请人： Carina Engstrand , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans Johansson , Jean-Luc Maloisel
发明人： Carina Engstrand , Annika Forss , Gunnar Glad , Bo-Lennart Johansson , Hans Johansson , Jean-Luc Maloisel
IPC分类号： C07K1/16 , C07C211/03 , G01N30/02
CPC分类号： B01D15/3828 , B01D15/327 , B01D15/363 , B01D15/3804 , B01D15/3809 , B01D15/3847 , B01J20/286 , B01J20/3253 , B01J20/3285 , B01J41/20 , C07K1/165 , C07K1/36 , C07K16/00 , C07K16/065
摘要： The present invention relates to a chromatography ligand defined by the following formula (I) R1—R2—N(R3)—R4—R5 wherein R1 is a substituted or non-substituted phenyl group; R2 is a hydrocarbon chain comprising 0-4 carbon atoms; R3 is a hydrocarbon chain comprising 1-3 carbon atoms; R4 is a hydrocarbon chain comprising 1-5 carbon atoms; and R5 is OH or H. The invention also comprises a separation matrix, comprising the described ligands coupled to a porous support, such as particles or a membrane. The ligand and matrix according to the invention is useful for purification of biomolecules or organic compounds, such as proteins, polypeptides, DNA etc. An advantageous use according to the invention is the purification of antibodies.
摘要翻译：本发明涉及由下式（I）定义的色谱配体R 1 -R 2 -N（R 3 N）-R 其中R 1是取代或未取代的苯基;其中R 1是取代或未取代的苯基; R 2是包含0-4个碳原子的烃链; R 3是包含1-3个碳原子的烃链; R 4是包含1-5个碳原子的烃链; 并且R 5是OH或H.本发明还包括分离基质，其包含与多孔载体如颗粒或膜偶联的所述配体。根据本发明的配体和基质可用于纯化生物分子或有机化合物，例如蛋白质，多肽，DNA等。根据本发明的有利用途是抗体的纯化。

27. 发明申请

US20070167613A1 Process for the purification of antibodies 有权
标题翻译：抗体纯化方法
公开(公告)号：US20070167613A1
公开(公告)日：2007-07-19
申请号：US10589717
申请日：2005-02-25
申请人： Bo-Lennart Johansson , Hans Johansson , Anna Gronberg , Jean-Luc Maloisel , Nicolas Thevenin
发明人： Bo-Lennart Johansson , Hans Johansson , Anna Gronberg , Jean-Luc Maloisel , Nicolas Thevenin
IPC分类号： C07K16/18
CPC分类号： B01J20/3253 , B01D15/3847 , B01J20/3242 , B01J20/3246 , B01J20/3255 , B01J20/3285
摘要： The present invention relates to a process for the purification of antibodies from one or more impurities in a liquid, which process comprises contacting said liquid with a first chromatography resin comprised of a support to which multi-modal ligands have been immobilised to adsorb the antibodies to the resin, wherein each multi-modal ligand comprises at least one cation-exchanging group and at least one aromatic or heteroaromatic ring system; adding an eluent to release the antibodies from the resin; and contacting the eluate so obtained with a second chromatography resin. In one embodiment, the ring-forming atoms of the aromatic or heteroaromatic entity are selected from the group consisting of C, S and O, and the cation exchanging group is a weak cation exchanger.
摘要翻译：本发明涉及一种从液体中的一种或多种杂质纯化抗体的方法，该方法包括使所述液体与包含多元配体的载体的第一层析树脂接触以将抗体吸附到所述树脂，其中每个多模式配体包含至少一个阳离子交换基团和至少一个芳族或杂芳族环系统; 添加洗脱液以从树脂中释放抗体; 并将如此获得的洗脱液与第二层析树脂接触。在一个实施方案中，芳族或杂芳族实体的成环原子选自C，S和O，阳离子交换基团是弱阳离子交换剂。

28. 发明申请

US20070125711A1 Novel separation matrix 审中-公开
标题翻译：新型分离矩阵
公开(公告)号：US20070125711A1
公开(公告)日：2007-06-07
申请号：US10575945
申请日：2004-11-01
申请人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Anders Ljunglof , Jean-Luc Maloisel
发明人： Jan Bergstrom , Gunnar Glad , Bo-Lennart Johansson , Anders Ljunglof , Jean-Luc Maloisel
IPC分类号： B01D15/08
CPC分类号： B01J20/3285 , B01D15/364 , B01J20/286 , B01J20/3244
摘要： The present invention relates to a separation matrix comprised of ligands coupled to the surfaces of a porous support, such as one or more porous particles, wherein the ligands provide at least one chemical gradient within the support. In the most advantageous embodiment, the chemical gradient is a ligand density gradient. The invention also relates to a method of providing a separation matrix comprising ligands coupled to the surfaces of a porous support, in which method at least one ligand density gradient is provided by solvent-controlled diffusion of at least one reagent into the porous support.
摘要翻译：本发明涉及由耦合到多孔载体的表面的配体（例如一种或多种多孔颗粒）组成的分离基质，其中配体在载体内提供至少一种化学梯度。在最有利的实施方案中，化学梯度是配体密度梯度。本发明还涉及一种提供包含耦合到多孔载体表面的配体的分离基质的方法，其中至少一种配体密度梯度由至少一种试剂的溶剂控制扩散提供到多孔载体中。

29. 发明授权

US06852230B2 Method for the manufacture of compositions containing low concentrations of salts 失效
标题翻译：制备含低浓度盐的组合物的方法
公开(公告)号：US06852230B2
公开(公告)日：2005-02-08
申请号：US10451195
申请日：2001-12-19
申请人： Makonnen Belew , Bo-Lennart Johansson , Jean-Luc Maloisel
发明人： Makonnen Belew , Bo-Lennart Johansson , Jean-Luc Maloisel
IPC分类号： A23L33/00 , A61K38/00 , A61K39/395 , A61P7/00 , A61P37/00 , B01J39/04 , B01J39/26 , B01J41/04 , B01J41/20 , B01J47/14 , B01J49/00 , C02F1/42 , C07K1/18 , B01D15/08
CPC分类号： B01J41/20 , B01J39/04 , B01J39/26 , B01J41/04
摘要： Method for desalting an aqueous liquid containing a charged substance comprising the steps of: (i) contacting liquid (I) with an ion-exchanger (1) under conditions permitting binding between ion-exchanger and substance, said ion-exchanger comprising a base matrix carrying an ion-exchange ligand (Ligand 1) with the opposite charge compared to the substance, (ii) desorbing said substance from said ion-exchanger by the use of a liquid (liquid (II)). Wherein: (A) ion-exchanger (1) is an ion-exchanger: (a) that can bind the substance in an aqueous reference liquid at ionic strength corresponding to 0.1 M NaCl, preferably 0.25 M NaCl; and (b) permits a breakthrough capacity at the pH provided by liquid (I) which is more than 2 mg/ml of gel, at a breakthrough of 10% and linear flow velocity of 300 cm/h; and (B) in step (ii) the pH of liquid (11) is adjusted to a pH value where the charge difference between the substance, the ligand and/or the ion-exchanger is lowered.
摘要翻译：含有带电物质的含水液体的脱盐方法包括以下步骤：（i）在允许离子交换剂和物质之间结合的条件下使液体（I）与离子交换剂（1）接触，所述离子交换剂包含基础基质携带与物质相反的电荷的离子交换配体（配体1），（ii）通过使用液体（液体（II））从所述离子交换器解吸所述物质。其中：（A）离子交换器（1）是离子交换剂：（a）可以以对应于0.1M NaCl，优选0.25M NaCl的离子强度将物质结合在水性参考液中; 和（b）允许在液体（I）提供的pH为10％以上且线性流速为300cm / h的情况下，其具有超过2mg / ml凝胶的pH的穿透能力; 和（B）在步骤（ii）中，将液体（11）的pH调节至其中物质，配体和/或离子交换剂之间的电荷差降低的pH值。

30. 发明授权

US09044740B2 Purification of immunoglobulins 有权
标题翻译：免疫球蛋白的纯化
公开(公告)号：US09044740B2
公开(公告)日：2015-06-02
申请号：US12594002
申请日：2008-03-20
申请人： Jean-Luc Maloisel , Bo-Lennart Johansson
发明人： Jean-Luc Maloisel , Bo-Lennart Johansson
IPC分类号： C07K16/00 , C07H1/00 , B01D15/38 , B01D15/08 , B01J20/289 , B01D15/32 , B01J20/286 , B01J20/32 , C07K1/20 , B01J20/26 , B01D15/36
CPC分类号： B01J20/289 , B01D15/32 , B01D15/327 , B01D15/361 , B01D15/3804 , B01D15/3847 , B01J20/262 , B01J20/267 , B01J20/286 , B01J20/3212 , B01J20/3219 , B01J20/3251 , B01J20/3272 , B01J20/328 , B01J20/3285 , B01J2220/54 , B01J2220/58 , C07K1/20 , C07K16/00
摘要： The present invention relates to a separation matrix comprised of a porous or non-porous support to which ligands have been immobilized, wherein said ligands comprise at least one aliphatic sulfamide. The invention also relates to a chromatography column that contains the described separation matrix, as well as to a method of isolating immunoglobulins, such as IgG, Fab fragments, fusion proteins containing immunoglobulins etc, by adsorption to a separation matrix that comprises the aliphatic sulfamide ligands of the invention.
摘要翻译：本发明涉及由已经固定有配体的多孔或无孔载体组成的分离基质，其中所述配体包含至少一个脂族磺酰胺。本发明还涉及含有所述分离基质的色谱柱，以及通过吸附到包含脂族磺酰胺配体的分离基质上分离免疫球蛋白如IgG，Fab片段，含有免疫球蛋白的融合蛋白等的方法的本发明。

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