专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

11. 发明授权

US5736394A Cellular uptake of modified peptides 失效
标题翻译：修饰肽的细胞摄取
公开(公告)号：US5736394A
公开(公告)日：1998-04-07
申请号：US642493
申请日：1996-05-03
申请人： Peter S. Coleman , Katherine Sheldon
发明人： Peter S. Coleman , Katherine Sheldon
IPC分类号： A61K38/00 , A61K51/08 , C07K1/107 , C07K14/82 , C12N5/00 , C07K5/00 , C07K7/00 , C07K17/00
CPC分类号： C07K14/82 , C07K1/1077 , A61K38/00 , Y10S435/963 , Y10S435/966 , Y10T436/2525
摘要： Disclosed herein is a cell containing a modified peptide. More specifically, the N-terminal amino acid residue of the peptide is modified by the addition of an aryl ketone group which, when contacted with an appropriate substrate, and exposed to light having a wavelength of about 330 nm or greater, results in the covalent bonding of the peptide to the substrate by a C--H insertion dominant mechanism. In preferred, embodiments, the aryl ketone is a benzophenone moiety. The peptide can be designed to specifically bind to a protein of interest in the cell. The cell is then contacted with light having a wavelength of greater than about 330 nm to bind the peptide covalently to the binding site on the intracellular protein of interest. In this way, the modified peptide can be used to specifically and irreversibly block a binding site on an intracellular protein of interest.
摘要翻译：本文公开了含有修饰肽的细胞。更具体地，肽的N-末端氨基酸残基通过加入芳基酮基进行改性，当芳基酮与适当的底物接触并暴露于波长为约330nm或更大的光时，导致共价通过CH插入显性机制将肽与底物结合。在优选的实施方案中，芳基酮是二苯甲酮部分。肽可以设计成特异性结合细胞中目标蛋白质。然后将细胞与波长大于约330nm的光接触，以将肽共价结合到感兴趣的细胞内蛋白质上的结合位点。以这种方式，修饰的肽可用于特异地和不可逆地阻断感兴趣的细胞内蛋白质上的结合位点。

12. 发明授权

US5723295A Methods, acridan compounds and kits for producing light 失效
标题翻译：方法，吖啶化合物和用于产生光的试剂盒
公开(公告)号：US5723295A
公开(公告)日：1998-03-03
申请号：US644088
申请日：1996-05-09
申请人： Hashem Akhavan-Tafti , Zahra Arghavani , Renuka DeSilva
发明人： Hashem Akhavan-Tafti , Zahra Arghavani , Renuka DeSilva
IPC分类号： C07D219/02 , C07D219/04 , C07D219/06 , C12Q1/28 , G01N33/535
CPC分类号： C07D219/06 , C07D219/02 , C07D219/04 , C12Q1/28 , Y10S435/966 , Y10S435/968 , Y10S435/975
摘要： A chemiluminescent assay method, compositions, kits and chemiluminescent acridan compounds are described which use a two-step chemiluminescent reaction process. The reaction involves an acridan compound, preferably a derivative of an N-alkylacridan-9-carboxylic acid, which undergoes a reaction with a peroxide compound, a peroxidase enzyme and an enhancer under conditions of time, temperature and pH which permit the accumulation of an intermediate compound, which is subsequently induced to produce a burst of light by raising the pH. The result is generation of very high intensity light from the reaction. The peroxidase enzyme is present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the hydrolytic enzyme is bound to a reporter molecule. The method is particularly amenable to automated assays because of the separation of the incubation and light generating steps.
摘要翻译：描述了使用两步化学发光反应过程的化学发光测定方法，组合物，试剂盒和化学发光的吖啶化合物。该反应涉及吖啶化合物，优选N-烷基吖啶-9-羧酸的衍生物，其在时间，温度和pH条件下与过氧化物，过氧化物酶和增强剂发生反应，这允许积聚中间体化合物，其随后通过提高pH而被诱导产生突发性的光。结果是从反应产生非常高强度的光。过氧化物酶在免疫测定，DNA探针测定或其他测定中单独存在或连接到特异性结合对的成员，其中水解酶与报道分子结合。由于孵育和光生成步骤的分离，该方法特别适用于自动化测定。

13. 发明授权

US4812395A Method for detecting enzymatic activity using particle agglutination 失效
公开(公告)号：US4812395A
公开(公告)日：1989-03-14
申请号：US877998
申请日：1986-08-19
申请人： Robert A. Ballas , William A. Frey
发明人： Robert A. Ballas , William A. Frey
IPC分类号： C12Q1/00 , C12Q1/34 , C12Q1/42 , G01N33/543 , G01N33/94 , G01N33/546 , G01N33/547
CPC分类号： C12Q1/34 , C12Q1/00 , C12Q1/42 , G01N33/54313 , G01N33/94 , Y10S435/966 , Y10S436/805
摘要： A method is disclosed for determining enzymatic activity in a liquid sample by particle agglutination or inhibition of particle agglutination.

14. 发明授权

US4810631A Signal enhancement in immunoassay by modulation of chemical catalysis 失效
标题翻译：通过调节化学催化免疫测定的信号增强
公开(公告)号：US4810631A
公开(公告)日：1989-03-07
申请号：US861818
申请日：1986-05-12
申请人： Michael E. Perlman , Susan A. Evans
发明人： Michael E. Perlman , Susan A. Evans
IPC分类号： G01N33/543 , G01N33/542 , G01N33/58 , G01N33/53 , C12Q1/44
CPC分类号： G01N33/542 , G01N33/581 , Y10S435/81 , Y10S435/966 , Y10S436/808 , Y10S436/821
摘要： A method for immunoassay for a ligand suspected to be present in a fluid includes use of an enzyme, a metal ion catalyst for an indicator reaction and a blocked modulator for the catalyst. Ligand present in the fluid binds to an antiligand. The resulting bound fraction activates the enzyme to unblock the modulator. The free modulator activates or inhibits the catalyst thereby modulating the rate of an indicator reaction between a substrate and a redox reagent. The presence of absence of the ligand in the fluid is indicated by a signal, such as a color change or a rate of color change, consequent to the indicator reaction. The invention includes a kit of materials useful for performing the method of the invention.
摘要翻译：怀疑存在于流体中的配体的免疫测定方法包括使用酶，用于指示剂反应的金属离子催化剂和催化剂的封闭调节剂。存在于液体中的配体与抗配体结合。所得到的结合部分激活酶以阻断调节剂。游离调节剂激活或抑制催化剂，从而调节底物和氧化还原剂之间的指示剂反应速率。流体中不存在配体的信号由指示剂反应所引起的诸如颜色变化或颜色变化率的信号表示。本发明包括可用于实施本发明方法的一组材料。

15. 发明授权

US4707441A Binding assays in automated apparatus with liposome compatible surfactants 失效
标题翻译：在与脂质体相容的表面活性剂的自动化装置中的结合测定
公开(公告)号：US4707441A
公开(公告)日：1987-11-17
申请号：US638596
申请日：1984-08-06
申请人： Syed I. Ahmad , Eddie Hedaya
发明人： Syed I. Ahmad , Eddie Hedaya
IPC分类号： G01N33/544 , A61K39/00 , G01N33/532 , G01N33/58 , G01N33/53 , G01N33/566 , G01N35/00
CPC分类号： G01N33/532 , G01N33/586 , Y10S435/966 , Y10S436/829 , Y10T428/2984 , Y10T436/11
摘要： Disclosed is a specific binding assay composition and method for determining a ligand in a sample. The composition comprises (a) a binding partner for the ligand; (b) a detection system which has at least two components; (c) a selectively accessible vesicle having a surface-incorporated ligand or ligand analog and a first component of the detection system therein; (d) a substance which modifies vesicle accessibility in response to binding of surface-incorporated ligand or ligand analog and the binding partner; (e) at least one additional component of the detection system which is reactive with the first component to produce a detectable response; and (f) at least one surfactant which does not modify vesicle accessibility. The composition and method are suitable for use with automated, including continuous flow-type, analyzers.
摘要翻译：公开了用于测定样品中配体的特异性结合测定组合物和方法。组合物包含（a）配体的结合配偶体; （b）具有至少两个部件的检测系统; （c）具有表面掺入的配体或配体类似物的选择性可接近的小泡和其中的检测系统的第一组分; （d）响应于表面掺入的配体或配体类似物和结合配偶体的结合而修饰囊泡可接近性的物质; （e）所述检测系统的至少一个另外的组分，其与所述第一组分反应以产生可检测的反应; 和（f）至少一种不改变囊泡可接近性的表面活性剂。该组合物和方法适用于自动化，包括连续流动型分析仪。

16. 发明授权

US4623620A Enucleated granulocytes, their preparation and use 失效
标题翻译：去核粒细胞，其制备和使用
公开(公告)号：US4623620A
公开(公告)日：1986-11-18
申请号：US519563
申请日：1983-08-02
申请人： Dirk Roos , Alwin A. Voetman , Louis J. Meerhof , Bertram Lubin
发明人： Dirk Roos , Alwin A. Voetman , Louis J. Meerhof , Bertram Lubin
IPC分类号： G01N33/50 , G01N33/531 , G01N33/569 , G01N33/53
CPC分类号： G01N33/531 , G01N33/5005 , G01N33/56972 , Y10S435/81 , Y10S435/966 , Y10S435/975
摘要： Granulocytes, particularly neutrophilic granulocytes, also called polymorphonuclear leukocytes (PMN), have been found to retain their immunological properties after enucleation. Moreover, in contrast with normal granulocytes, enucleated granulocytes are stable when stored in frozen condition. The invention relates to the enucleated granulocytes, especially in frozen condition, to a process for preparing the enucleated granulocytes, to a process for assaying antibodies against granulocytes, and to a kit for carrying out the latter process.
摘要翻译：已经发现粒细胞，特别是嗜中性粒细胞，也称为多形核白细胞（PMN），其在摘除后保留其免疫学性质。此外，与正常粒细胞相反，当冷冻条件下储存时，去核粒细胞是稳定的。本发明涉及脱粒的粒细胞，特别是在冷冻条件下，涉及制备去核粒细胞的方法，用于测定针对粒细胞的抗体的方法，以及用于进行后一过程的试剂盒。

17. 发明授权

US4619903A Process for carrying out serological investigations according to the principle of the complement fixation test, and test packages for use in this process 失效
标题翻译：根据补体固定试验的原理进行血清学检查的过程，以及用于该过程的试验包装
公开(公告)号：US4619903A
公开(公告)日：1986-10-28
申请号：US485064
申请日：1983-04-14
申请人： Dezzo S. Bartos , Denis Fitzpatrick
发明人： Dezzo S. Bartos , Denis Fitzpatrick
IPC分类号： G01N33/536 , G01N33/53 , G01N33/557 , G01N33/566 , G01N33/563
CPC分类号： G01N33/557 , Y10S435/962 , Y10S435/966 , Y10S436/821
摘要： Serological investigations according to the principle of the Complement Fixation Test (CFT) can be evaluated by means of a kinetic method by using an excess of complement. Preferably the amount of the complement in the reagents and the control samples are balanced against one another.
摘要翻译：根据补体固定试验（CFT）原理进行血清学检查可以通过使用过量的补体的动力学方法进行评估。优选地，试剂和对照样品中的补体的量彼此平衡。

18. 发明授权

US4536479A Use of anti-idiotype antibodies in immunoassays 失效
标题翻译：在免疫测定中使用抗独特型抗体
公开(公告)号：US4536479A
公开(公告)日：1985-08-20
申请号：US477611
申请日：1983-03-22
申请人： Ronald Vander-Mallie
发明人： Ronald Vander-Mallie
IPC分类号： G01N33/542 , C12Q1/26 , C12Q1/28 , G01N33/543 , G01N33/68 , G01N33/54 , G01N33/52
CPC分类号： G01N33/686 , G01N33/54313 , Y10S435/965 , Y10S435/966 , Y10S436/80 , Y10S436/819
摘要： The use of anti-idiotype antibodies as functional substitutes for antigens or haptens in immunoassays is disclosed.
摘要翻译：公开了使用抗独特型抗体作为免疫测定中的抗原或半抗原的功能性替代物。

19. 发明授权

US4495296A Labeled anti-hapten antibodies and their use as a universal reagent for solid phase radio- and/or enzyme-immunoassays 失效
标题翻译：标记的抗半抗原抗体及其作为固相放射和/或酶免疫测定的通用试剂的用途
公开(公告)号：US4495296A
公开(公告)日：1985-01-22
申请号：US41127
申请日：1979-05-21
申请人： A. Robert Neurath , Nathan Strick
发明人： A. Robert Neurath , Nathan Strick
IPC分类号： G01N33/532 , G01N33/543 , G01N33/52 , G01N33/54 , G01N33/56
CPC分类号： G01N33/532 , G01N33/54306 , Y10S435/966 , Y10S436/804 , Y10S436/822
摘要： A process for detecting the presence of an antigen in a specimen is described, which process comprises:(A) contacting said specimen with a substrate coated with antibodies of said antigen, incubating the contacted substrate and washing the substrate;(B) contacting the washed material of step (A) with a hapten conjugated antibody against said antigen, incubating the so-contacted material and washing the so-incubated material;(C) contacting the washed material of step (B) with a radioactive material labeled or enzyme containing anti-hapten antibody, incubating the so-contacted material and washing the same; and(D) effecting radioimmunoassay if said antibody is radioactive or enzyme labeled immunoassay if said antibody contains an enzyme moiety.Quantitative determination of the antigen in the specimen is effected by comparing the counts of the radioimmunoassay or the concentration of enzyme against a standard as by photocolormetric methods.
摘要翻译：描述了用于检测样品中抗原存在的方法，该方法包括：（A）使所述样品与涂有所述抗原抗体的底物接触，孵育接触的基质并洗涤底物; （B）使所洗涤的步骤（A）的材料与针对所述抗原的半抗原共轭抗体接触，培养如此接触的材料并洗涤如此温育的材料; （C）使经过洗涤的步骤（B）的材料与标记或含有抗半抗原抗体的酶的放射性物质接触，孵育如此接触的材料并洗涤; 和（D）如果所述抗体是放射性的，则进行放射免疫测定，如果所述抗体含有酶部分则进行酶标记的免疫测定。通过比较放射免疫测定的计数或酶的浓度与标准方法的比较，可以定量测定样品中的抗原。

20. 发明授权

US4492751A Heterogenous specific binding assay employing an enzyme substrate as label 失效
标题翻译：使用酶底物作为标记的异源特异性结合测定
公开(公告)号：US4492751A
公开(公告)日：1985-01-08
申请号：US84867
申请日：1979-10-15
申请人： Robert C. Boguslaski , Robert J. Carrico , James E. Christner
发明人： Robert C. Boguslaski , Robert J. Carrico , James E. Christner
IPC分类号： C07D209/48 , C07D237/32 , C07D495/04 , C07H19/20 , G01N33/535 , G01N33/537 , G01N33/54 , G01N33/52 , G01N33/58
CPC分类号： C07D495/04 , C07D209/48 , C07D237/32 , C07H19/20 , G01N33/535 , G01N33/537 , Y10S435/966 , Y10S435/968 , Y10S435/97 , Y10S435/975 , Y10S436/80 , Y10S436/817
摘要： An improved heterogenous specific binding assay method which employs a substance having reactant activity, i.e., a reactant, as a labeling substance in the detection of a ligand in a liquid medium. The method is carried out using reagent means which comprises, as its labeled constituent, a conjugate formed of a specific binding substance coupled to the reactant. The reactant advantageously is an enzymatic reactant such as an enzyme substrate or coenzyme. The activity of the conjugated reactant as a constituent of a predetermined reaction system is utilized as means for monitoring the extent of binding of the labeled constituent in conventional heterogenous specific binding assay schemes. The presence of a ligand in a liquid medium may be determined following conventional competitive binding manipulative techniques. After the necessary separation of the bound- and free-phases resulting in the specific binding reaction system, the extent of binding of the labeled constituent is determined by contacting either phase with the necessary materials to form the predetermined monitoring reaction system in which the labeling substance is active and assessing reactant activity therein.
摘要翻译：一种改进的异源特异性结合测定方法，其使用具有反应活性的物质即反应物作为液体介质中配体检测中的标记物质。该方法使用试剂装置进行，该试剂装置包含作为其标记成分的由与反应物偶联的特异性结合物质形成的缀合物。反应物有利地是酶反应物，例如酶底物或辅酶。缀合的反应物作为预定反应体系的组分的活性被用作监测常规异源特异性结合测定方案中标记组分的结合程度的方法。配体在液体介质中的存在可以按照常规的竞争性结合操纵技术来测定。在产生特异性结合反应体系的结合和自由相应的必要分离之后，标记组分的结合程度通过使任一相与所需材料接触来形成预定的监测反应体系，其中标记物质是活性的并评估其中的反应物活性。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式