专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

11. 发明申请

US20170137863A1 Self-Immolative Probes for Enzyme Activity Detection 审中-公开
公开(公告)号：US20170137863A1
公开(公告)日：2017-05-18
申请号：US15416119
申请日：2017-01-26
申请人： ENZO LIFE SCIENCES, INC., C/O ENZO BIOCHEM, INC.
发明人： IRINA LEBEDEVA , WAYNE FORREST PATTON , MACIEJ SZCZEPANIK , YUEJUN XIANG , PRAVEEN PANDE
IPC分类号： C12Q1/26 , C09K11/06 , C07D491/052 , C07D491/20 , C07D493/10 , C07D311/08
CPC分类号： C12Q1/26 , C07D311/08 , C07D491/052 , C07D491/20 , C07D493/10 , C09K11/06 , C09K2211/1022 , C09K2211/1088 , C12Q1/37 , C12Q1/44 , C12Q1/485 , G01N2333/902 , G01N2500/00 , Y10T436/18
摘要： Provided is a compound having the structure: (SIG)-(SI-MOD)m where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.

12. 发明申请

US20170122954A1 PROFILING REACTIVE OXYGEN, NITROGEN AND HALOGEN SPECIES 审中-公开
公开(公告)号：US20170122954A1
公开(公告)日：2017-05-04
申请号：US15402505
申请日：2017-01-10
申请人： ENZO LIFE SCIENCES, INC., C/O ENZO BIOCHEM, INC
发明人： IRINA LEBEDEVA , WAYNE FORREST PATTON
IPC分类号： G01N33/58 , G01N15/14 , G01N33/50
CPC分类号： G01N33/582 , C12Q1/02 , G01N15/14 , G01N33/5005
摘要： Provided are methods, kits and systems for simultaneously profiling of global reactive species and selected reactive species, such as global and specific reactive oxygen species (ROS), reactive nitrogen species (RNS), reactive halogen species (RHS) or combinations thereof through multiplexed fluorescence detection of three or more compatible indicator probes in live cells or subcellular organelles.

13. 发明申请

US20150192564A1 PROFILING REACTIVE OXYGEN, NITROGEN AND HALOGEN SPECIES 审中-公开
标题翻译：配制反应性氧，氮和卤素物种
公开(公告)号：US20150192564A1
公开(公告)日：2015-07-09
申请号：US14629931
申请日：2015-02-24
申请人： ENZO LIFE SCIENCES, INC., C/O ENZO BIOCHEM, INC
发明人： IRINA LEBEDEVA , WAYNE FORREST PATTON
IPC分类号： G01N33/50 , G01N33/58
CPC分类号： G01N33/582 , C12Q1/02 , G01N15/14 , G01N33/5005
摘要： This invention provides methods, kits and systems which permit simultaneous profiling of multiple reactive oxygen species (ROS), reactive nitrogen species (RNS) and/or reactive halogen species (RHS) including reactive chlorine species (RCS) and/or reactive bromine species (RBS) through multiplexed fluorescence detection of three or more indicator probes in live cells or subcellular organelles.
摘要翻译：本发明提供方法，试剂盒和系统，其允许包括反应性氯物质（RCS）和/或反应性溴物质（RCS）和/或反应性溴物质的多种活性氧（ROS），活性氮物质（RNS）和/或反应性卤素物质（RHS） RBS）通过活细胞或亚细胞器中的三个或更多个指示物探针的多重荧光检测。

14. 发明申请

US20150191768A1 SELF-IMMOLATIVE PROBES FOR ENZYME ACTIVITY DETECTION 有权
标题翻译：用于酶活性检测的自我检测
公开(公告)号：US20150191768A1
公开(公告)日：2015-07-09
申请号：US14447817
申请日：2014-07-31
申请人： Enzo Life Sciences, Inc. c/o Enzo Biochem, Inc.
发明人： MACIEJ SZCZEPANIK , IRINA V. LEBEDEVA , YUEJUN XIANG , PRAVEEN PANDE , WAYNE FORREST PATTON
IPC分类号： C12Q1/48 , C12Q1/44 , C12Q1/37
CPC分类号： C12Q1/26 , C07D311/08 , C07D491/052 , C07D491/20 , C07D493/10 , C09K11/06 , C09K2211/1022 , C09K2211/1088 , C12Q1/37 , C12Q1/44 , C12Q1/485 , G01N2333/902 , G01N2500/00 , Y10T436/18
摘要： Provided is a compound comprising the structure: (SIG)-(SI-MOD)m. In this compound, SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. With this compound, when MOD is modified by an activator, SI is destabilized and self cleaved from SIG such that SIG generates an increased signal. Also provided is a method of determining whether a sample comprises an activator, using the above-described compound. Additionally provided is a method of determining whether a cell comprises a nitroreductase using the above-described compound where nitroreductase is the activator. Further provided is a method of determining whether a mammalian cell is hypoxic using the above-described compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase, using the above-described compound where nitroreductase is the activator, is also provided. Also provided is a method of identifying nitroreductase in a sample, using the above-described compound where nitroreductase is the activator.
摘要翻译：提供了包含以下结构的化合物：（SIG） - （SI-MOD）m。在该化合物中，SIG是信号分子，SI是与SIG结合的自消除结构，使得SIG相对于没有SI的SIG的信号具有降低的信号，MOD是与SI结合的部分，其经受活化剂，m为1至约10的整数。用该化合物，当MOD被活化剂修饰时，SI是不稳定的并且从SIG自我裂解，使得SIG产生增加的信号。还提供了使用上述化合物来确定样品是否包含活化剂的方法。另外提供了使用上述硝基还原酶是活化剂的化合物来确定细胞是否包含硝基还原酶的方法。还提供了使用上述硝基还原酶是活化剂的化合物来确定哺乳动物细胞是否缺氧的方法。还提供了使用上述硝基还原酶为活化剂的化合物检测包含硝基还原酶的微生物的方法。还提供了使用上述硝基还原酶是活化剂的化合物来鉴定样品中的硝基还原酶的方法。

15. 发明申请

US20130045480A1 Novel dyes, composite dyes, and oligonucleotides or polynucleotides comprising such dyes - useful or detection or quantification of desirable target molecules 有权
标题翻译：新型染料，复合染料和寡核苷酸或包含这种染料的多核苷酸 - 可用或检测或定量所需的靶分子
公开(公告)号：US20130045480A1
公开(公告)日：2013-02-21
申请号：US12456515
申请日：2009-06-17
申请人： Yuejun Xiang , Praveen Pande , Rajesh Khazanchi , Elazar Rabbani , Dakai Liu , Wei Cheng
发明人： Yuejun Xiang , Praveen Pande , Rajesh Khazanchi , Elazar Rabbani , Dakai Liu , Wei Cheng
IPC分类号： C07D417/14 , C07D277/64 , C07D417/12 , G01N21/76 , C07H21/00 , C07K16/00 , C07K17/02 , C12N9/96 , C07K14/575 , C07K14/52 , C07H15/26 , C08B37/00 , C12N1/20 , C07F7/02 , C07D455/04
CPC分类号： C07D417/14 , C07D209/14 , C07D235/20 , C07D401/14 , C07D405/14 , C07D417/06 , C07H19/10 , C07H21/00 , C07K14/00 , C07K16/00 , C09B23/0091 , C09B23/04 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/145 , C09B23/146
摘要： The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins and nucleic acids. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified by the addition of charged and polar groups to provide beneficial properties.
摘要翻译：本发明提供可用于检测或定量所需靶分子例如蛋白质和核酸的染料，活性染料和标记试剂。提供可以在染料与靶分子的结合提供信号产生的溶液中游离的染料。还提供了包含可用于将染料连接到将与所需靶分子结合的探针的反应性基团的染料。通过加入带电极和极性基团来改进本发明的新型染料以提供有益的性质。

16. 发明申请

US20120040430A1 Compositions useful for detection or quantification of desirable target molecules, novel dyes, composite dyes, and oligonucleotides or polynucleotides comprising such dyes 有权
标题翻译：用于检测或定量所需靶分子，新染料，复合染料和寡核苷酸的组合物或包含这些染料的多核苷酸
公开(公告)号：US20120040430A1
公开(公告)日：2012-02-16
申请号：US12456513
申请日：2009-06-17
申请人： Yuejun Xiang , Praveen Pande , Rajesh Khazanchi , Elazar Rabbani , Dakai Liu , Wei Cheng
发明人： Yuejun Xiang , Praveen Pande , Rajesh Khazanchi , Elazar Rabbani , Dakai Liu , Wei Cheng
IPC分类号： C09B23/10
CPC分类号： C07D417/14 , C07D209/14 , C07D235/20 , C07D401/14 , C07D405/14 , C07D417/06 , C07H19/10 , C07H21/00 , C07K14/00 , C07K16/00 , C09B23/0091 , C09B23/04 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/145 , C09B23/146
摘要： The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins and nucleic acids. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified by the addition of charged and polar groups to provide beneficial properties.
摘要翻译：本发明提供可用于检测或定量所需靶分子例如蛋白质和核酸的染料，活性染料和标记试剂。提供可以在染料与靶分子的结合提供信号产生的溶液中游离的染料。还提供了包含可用于将染料连接到将与所需靶分子结合的探针的反应性基团的染料。通过加入带电极和极性基团来改进本发明的新型染料以提供有益的性质。

17. 发明申请

US20100041020A1 Methods for detecting the presence, location or quantity of targets using novel dyes 有权
标题翻译：使用新型染料检测靶的存在，位置或数量的方法
公开(公告)号：US20100041020A1
公开(公告)日：2010-02-18
申请号：US12456503
申请日：2009-06-17
申请人： Yuejun Xiang , Praveen Pande , Rajesh Khazanchi , Elazar Rabbani , Dakai Liu , Wei Cheng
发明人： Yuejun Xiang , Praveen Pande , Rajesh Khazanchi , Elazar Rabbani , Dakai Liu , Wei Cheng
IPC分类号： G01N33/566 , G01N33/573 , C12Q1/70 , C12Q1/02 , G01N33/567 , C12Q1/68
CPC分类号： C07D417/14 , C07D209/14 , C07D235/20 , C07D401/14 , C07D405/14 , C07D417/06 , C07H19/10 , C07H21/00 , C07K14/00 , C07K16/00 , C09B23/0091 , C09B23/04 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/145 , C09B23/146
摘要： The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins and nucleic acids. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified by the addition of charged and polar groups to provide beneficial properties.
摘要翻译：本发明提供可用于检测或定量所需靶分子例如蛋白质和核酸的染料，活性染料和标记试剂。提供可以在染料与靶分子的结合提供信号产生的溶液中游离的染料。还提供了包含可用于将染料连接到将与所需靶分子结合的探针的反应性基团的染料。通过加入带电极和极性基团来改进本发明的新型染料以提供有益的性质。

18. 发明授权

US07323571B2 Heterodimeric dye composition 有权
标题翻译：异二聚染料组合物
公开(公告)号：US07323571B2
公开(公告)日：2008-01-29
申请号：US10764389
申请日：2004-01-23
申请人： Jannis G. Stavrianopoulos , Elazar Rabban
发明人： Jannis G. Stavrianopoulos , Elazar Rabban
IPC分类号： C07D293/00 , C07D291/00 , C07D207/00 , C07H21/02 , C07H21/04
CPC分类号： C07H21/00 , C09B23/02 , C09B47/00 , C09B57/00 , Y10S436/80
摘要： This invention provides for labeling reagents, labeled targets and processes for preparing labeling reagents. The labeling reagents can take the form of cyanine dyes, xanthene dyes, porphyrin dyes, coumarin dyes or composite dyes. These labeling reagents are useful for labeling probes or targets, including nucleic acids and proteins. These reagents can be usefully applied to protein and nucleic acid probe based assays. They are also applicable to real-time detection processes.
摘要翻译：本发明提供标记试剂，标记的靶标和制备标记试剂的方法。标记试剂可以采用花青染料，呫吨染料，卟啉染料，香豆素染料或复合染料的形式。这些标记试剂可用于标记探针或靶标，包括核酸和蛋白质。这些试剂可用于基于蛋白质和基于核酸探针的测定。它们也适用于实时检测过程。

19. 发明授权

US06992180B1 Oligo- or polynucleotides comprising phosphate-moiety labeled nucleotides 失效
标题翻译：包含磷酸酯部分标记的核苷酸的寡核苷酸或多核苷酸
公开(公告)号：US06992180B1
公开(公告)日：2006-01-31
申请号：US08479997
申请日：1995-06-07
申请人： Dean Engelhardt , Elazar Rabbani , Stanley Kline , Janes G. Stavrianopoulos , Dollie Kirtikar
发明人： Dean Engelhardt , Elazar Rabbani , Stanley Kline , Janes G. Stavrianopoulos , Dollie Kirtikar
IPC分类号： C12N15/11
CPC分类号： C12Q1/6816 , A61K47/62 , C07B59/008 , C07H19/04 , C07H19/10 , C07K17/06 , C12Q1/6804 , C12Q2525/117
摘要： The present invention provides a nucleotide having the formula, Sig-PM-SM-BASE wherein PM is a phosphate moiety, SM is a sugar moiety and BASE is a pyrimidine, purine or 7-deazapurine moiety. PM is attached to the 3′ or the 5′ position of the sugar moiety when the nucleotide is a deoxyribonucleotide and at the 2′, 3′ or 5′ position when the nucleotide is a ribonucleotide. BASE is attached to the 1′ position of SM from the N1 position when BASE is a pyrmidine or the N9 position when BASE is a purine or 7-deazapurine. Sig is covalently attached to PM directly or via a chemical linkage, and represents a detectable moiety covalently attached to SM directly or through a linkage group. Also provided are an oligo- or polynucleotide comprising at least one such phosphate-moiety labeled nucleotide, and other compositions including those wherein a polypeptide is terminally ligated or attached to the oligo- or polynucleotide. The phosphate-moiety labeled nucleotide, and the oligo- or polynucleotides and other compositions comprising such phosphate-moiety labeled nucleotides, are useful as diagnostic tools for detecting analytes and as therapeutic agents.
摘要翻译：本发明提供了具有下式的核苷酸：<？in-line-formula description =“In-line Formulas”end =“lead”？> Sig-PM-SM-BASE <？in-line-formula description =“In 其中PM是磷酸酯部分，SM是糖部分，BASE是嘧啶，嘌呤或7-脱氮嘌呤部分。当核苷酸是脱氧核糖核苷酸时，当核苷酸是核糖核苷酸时，PM连接到糖部分的3'或5'位置，并且在2'，3'或5'位置。当BASE是嘌呤或7-脱氮嘌呤时，当BASE是嘧啶或N 9位时，BASE从N <1>位置连接到SM的1'位置。 Sig直接或通过化学键共价连接到PM，并且代表可直接或通过连接基共价连接到SM的可检测部分。还提供了包含至少一个这样的磷酸酯部分标记的核苷酸的寡核苷酸或多核苷酸，以及包括多肽终止连接或连接到寡核苷酸或多核苷酸的那些的其它组合物。磷酸酯部分标记的核苷酸以及包含这种磷酸酯部分标记的核苷酸的寡核苷酸或多核苷酸和其它组合物可用作检测分析物和作为治疗剂的诊断工具。

20. 发明申请

US20170219612A1 HYDROXYCHOLESTEROL IMMUNOASSAY 审中-公开
公开(公告)号：US20170219612A1
公开(公告)日：2017-08-03
申请号：US15484754
申请日：2017-04-11
申请人： ENZO LIFE SCIENCES, INC. C/O ENZO BIOCHEM, INC.
发明人： Michael C. Mullenix , ROBERT ELLIOT ZIPKIN , JEFFREY KROLL ADAMS , WAYNE FORREST PATTON , JAMES J. DONEGAN
IPC分类号： G01N33/92 , C07J43/00
CPC分类号： G01N33/92 , C07J9/00 , C07J43/003 , C07K16/18 , C07K16/44 , C07K2317/21 , G01N33/5308 , G01N33/56966 , G01N2800/2835 , G01N2800/285 , G01N2800/52
摘要： Provided is a derivative of 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol. Also provided is a protein conjugated to the above derivative. Further provided is an antibody composition comprising antibodies that specifically bind to 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol. Additionally, a method of making antibodies that specifically bind to 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol is provided. Also, a method of assaying for 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol is provided. Additionally provided is a kit for detecting 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol. A method of detecting an enzyme or enzymes utilized in phase II drug metabolism is also provided. Also, a method of detecting an enzyme that synthesizes 22-hydroxycholesterol, 24S-hydroxycholesterol, 25-hydroxycholesterol, 26-hydroxycholesterol or 27-hydroxycholesterol is provided. Further provided is a method of evaluating progression of multiple sclerosis in a patient. Also provided is a method of determining whether a treatment for multiple sclerosis in a patient is effective. Further, a method of evaluating progression of Huntington's disease in a patient is provided. Additionally provided is a method of determining whether a treatment for Huntington's disease in a patient is effective.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式