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11. 发明申请

US20120309084A1 MICROCHIP FOR NUCLEIC ACID AMPLIFICATION REACTION AND A METHOD OF MANUFACTURING THE SAME 审中-公开
标题翻译：用于核酸放大反应的微波炉及其制造方法
公开(公告)号：US20120309084A1
公开(公告)日：2012-12-06
申请号：US13576898
申请日：2011-02-01
申请人： Hidetoshi Watanabe , Tomoteru Abe , Tasuku Yotoriyama
发明人： Hidetoshi Watanabe , Tomoteru Abe , Tasuku Yotoriyama
IPC分类号： C12M1/40 , B32B37/12 , B05D5/10
CPC分类号： C12Q1/6844 , B01L3/502707 , B01L2200/0689 , B01L2200/16 , B01L2300/0816 , B01L2300/0864 , C12Q1/6806 , G01N27/447 , C12Q2521/101 , C12Q2521/543 , C12Q2565/629
摘要： To provide a microchip for a nucleic acid amplification reaction which allows high-precision analysis by a simple method.There is provided a microchip A for a nucleic acid amplification reaction including an entrance through which a liquid enters from the outside, a plurality of wells configured to function as reaction sites of nucleic acid amplification reaction, and flow channels through which the liquid entered from the entrance is fed into each of the wells, in which a plurality of reagents needed for the reaction are laminated and anchored in a prescribed order in each well.
摘要翻译：提供用于核酸扩增反应的微芯片，其允许通过简单方法进行高精度分析。提供了用于核酸扩增反应的微芯片A，其包括液体从外部进入的入口，被配置为用作核酸扩增反应的反应位点的多个孔以及从其中进入的液体的流动通道将入口进料到每个孔中，其中反应所需的多种试剂在每个孔中以规定的顺序层压并锚定。

12. 发明申请

US20120070841A1 NUCLEIC ACID QUANTIFICATION METHOD AND MICROCHIP FOR NUCLEIC ACID AMPLIFICATION REACTION 失效
标题翻译：核酸扩增反应的核酸定量方法和微波
公开(公告)号：US20120070841A1
公开(公告)日：2012-03-22
申请号：US13231179
申请日：2011-09-13
申请人： Tomoteru Abe , Yuji Segawa , Junji Kajihara , Tomohiko Nakamura , Masaki Sato
发明人： Tomoteru Abe , Yuji Segawa , Junji Kajihara , Tomohiko Nakamura , Masaki Sato
IPC分类号： C12Q1/68 , C12M1/40
CPC分类号： B01L3/5027 , B01L7/52 , B01L2200/0694 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/0883
摘要： A nucleic acid quantification method that uses a microchip for nucleic acid amplification reaction, the microchip including an inlet through which a liquid is introduced from outside, a plurality of reaction regions provided as reaction sites of a nucleic acid amplification reaction, and a channel through which the liquid introduced through the inlet is supplied into each of the reaction regions, wherein the likelihood of the nucleic acid amplification reaction varies between the reaction regions, includes: flowing a detection target nucleic acid chain-containing solution through the channel and introducing the solution into each of the reaction regions to perform a nucleic acid amplification reaction; and detecting an amplification product in each of the reaction regions to specify the reaction regions in which the nucleic acid amplification reaction occurred.
摘要翻译：一种使用微芯片进行核酸扩增反应的核酸定量方法，所述微芯片包括从外部引入液体的入口，作为核酸扩增反应的反应位点提供的多个反应区域，通过入口引入的液体被供应到每个反应区域中，其中核酸扩增反应在反应区域之间变化的可能性包括：使含有检测目标的含有核酸链的溶液流过该通道并将溶液引入每个反应区进行核酸扩增反应; 并检测每个反应区域中的扩增产物，以指定发生核酸扩增反应的反应区域。

13. 发明授权

US08637251B2 Nucleic acid quantification method and microchip for nucleic acid amplification reaction 失效
标题翻译：核酸定量方法和微芯片进行核酸扩增反应
公开(公告)号：US08637251B2
公开(公告)日：2014-01-28
申请号：US13231179
申请日：2011-09-13
申请人： Tomoteru Abe , Yuji Segawa , Junji Kajihara , Tomohiko Nakamura , Masaki Sato
发明人： Tomoteru Abe , Yuji Segawa , Junji Kajihara , Tomohiko Nakamura , Masaki Sato
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： B01L3/5027 , B01L7/52 , B01L2200/0694 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2300/0883
摘要： A nucleic acid quantification method that uses a microchip for nucleic acid amplification reaction, the microchip including an inlet through which a liquid is introduced from outside, a plurality of reaction regions provided as reaction sites of a nucleic acid amplification reaction, and a channel through which the liquid introduced through the inlet is supplied into each of the reaction regions, wherein the likelihood of the nucleic acid amplification reaction varies between the reaction regions, includes: flowing a detection target nucleic acid chain-containing solution through the channel and introducing the solution into each of the reaction regions to perform a nucleic acid amplification reaction; and detecting an amplification product in each of the reaction regions to specify the reaction regions in which the nucleic acid amplification reaction occurred.
摘要翻译：一种使用微芯片进行核酸扩增反应的核酸定量方法，所述微芯片包括从外部引入液体的入口，作为核酸扩增反应的反应位点提供的多个反应区域，通过入口引入的液体被供应到每个反应区域中，其中核酸扩增反应在反应区域之间变化的可能性包括：使含有检测目标的含有核酸链的溶液流过该通道并将溶液引入每个反应区进行核酸扩增反应; 并检测每个反应区域中的扩增产物，以指定发生核酸扩增反应的反应区域。

14. 发明授权

US08691559B2 Micro channel, device for recovering nucleic acid and method for recovering nucleic acid 有权
标题翻译：微通道，用于回收核酸的装置和用于回收核酸的方法
公开(公告)号：US08691559B2
公开(公告)日：2014-04-08
申请号：US11858595
申请日：2007-09-20
申请人： Michihiro Ohnishi , Yuta Kan , Tomoteru Abe
发明人： Michihiro Ohnishi , Yuta Kan , Tomoteru Abe
IPC分类号： C12M1/34 , C12M3/00 , C12Q1/68 , B01D15/08 , C02F1/28 , C07H21/02 , C07H1/06
CPC分类号： C12N15/1006
摘要： A micro channel for recovering nucleic acid from a biological sample treated with a chaotropic ion, include an integrated portion composed of silica micro beads having a pore size of from 6 to 29 nm is formed in the channel.
摘要翻译：用离心力离子处理的生物样品回收核酸的微通道包括在通道中形成孔径为6〜29nm的二氧化硅微珠组成的整体部分。

15. 发明申请

US20070267336A1 Microflow path system 审中-公开
标题翻译：微流道系统
公开(公告)号：US20070267336A1
公开(公告)日：2007-11-22
申请号：US11798890
申请日：2007-05-17
申请人： Michihiro Ohnishi , Tomoteru Abe
发明人： Michihiro Ohnishi , Tomoteru Abe
IPC分类号： B01D15/08 , G01N30/02 , C12M1/34
CPC分类号： B01L3/502746 , B01L3/502753 , B01L3/561 , B01L2300/0681 , B01L2300/0838 , B01L2400/0487 , B01L2400/084 , G01N30/603
摘要： A microflow path system has a column layer of at least 0.5 mm in internal diameter. The column layer includes a hybrid-forming section and a fluid-supply promoting section arranged in a region downstream of the hybrid-forming section. The hybrid-forming section is packed with beads to provide a bead bed length of not longer than 4 mm. The fluid-supply promoting section serves to contribute to an increase in a flow rate of a fluid supply.
摘要翻译：微流路系统具有内径至少为0.5mm的柱层。柱层包括混合形成部分和设置在混合成形部分下游的区域中的流体供应促进部分。混合成形部分装有珠，以提供不超过4mm的珠床长度。流体供应促进部分有助于增加流体供应的流量。

16. 发明申请

US20100184067A1 PRIMER EVALUATION METHOD, PRIMER EVALUATION PROGRAM, AND REAL-TIME POLYMERASE CHAIN REACTION APPARATUS 审中-公开
标题翻译：初步评估方法，初步评估方案和实时聚合酶链反应装置
公开(公告)号：US20100184067A1
公开(公告)日：2010-07-22
申请号：US12686852
申请日：2010-01-13
申请人： Tomoteru Abe , Kazuhiro Nakagawa , Satomi Tanaka
发明人： Tomoteru Abe , Kazuhiro Nakagawa , Satomi Tanaka
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： B01L7/52 , B01L7/54 , B01L2200/147 , B01L2300/0654 , B01L2300/0819 , B01L2300/1822 , C12Q1/6851 , C12Q2527/101
摘要： A primer evaluation method includes: acquiring a signal indicating time change in an amplification amount obtained when sample sets prepared for the number of temperature conditions that should be made different from each other at an annealing stage in units of target nucleic acids diluted in a stepwise manner are so amplified that a temperature condition at a stage other than the annealing stage is fixed; acquiring a signal indicating initial amounts of the target nucleic acids diluted in a stepwise manner; obtaining amplification efficiency for each of the temperature conditions based on the time change in the amplification amount and the initial amount, and calculating a variation degree of the amplification efficiency; and submitting the variation degree and a reference value for quality evaluation of a primer, set with respect to the variation degree.
摘要翻译：引物评价方法包括：获取当以按步进方式稀释的目标核酸为单位的退火阶段对于应该使彼此不同的温度条件数量准备的样品组获得的扩增量的时间变化信号被放大使得在退火阶段之外的阶段的温度条件是固定的; 获取表示以逐步方式稀释的靶核酸的初始量的信号; 基于放大量和初始量的时间变化获得每个温度条件的放大效率，并计算放大效率的变化程度; 并提交相对于变化程度设定的引物的质量评价的变化程度和参考值。

17. 发明申请

US20080161553A1 MICRO CHANNEL, DEVICE FOR RECOVERING NUCLEIC ACID AND METHOD FOR RECOVERING NUCLEIC ACID 有权
标题翻译：微通道，用于回收核酸的装置和用于回收核酸的方法
公开(公告)号：US20080161553A1
公开(公告)日：2008-07-03
申请号：US11858595
申请日：2007-09-20
申请人： Michihiro Ohnishi , Yuta Kan , Tomoteru Abe
发明人： Michihiro Ohnishi , Yuta Kan , Tomoteru Abe
IPC分类号： C07H1/06 , C12M1/00 , C40B60/14
CPC分类号： C12N15/1006
摘要： A micro channel for recovering nucleic acid from a biological sample treated with a chaptropic ion, include an integrated portion composed of silica micro beads having a pore size of from 6 to 29 nm is formed in the channel.
摘要翻译：用于从由chaptropic离子处理的生物样品中回收核酸的微通道包括在通道中形成具有6至29nm孔径的二氧化硅微珠组成的整体部分。

18. 发明申请

US20110059541A1 Method for Obtaining Information on Formation of Double-Stranded Nucleic Acid 审中-公开
标题翻译：获得双链核酸形成信息的方法
公开(公告)号：US20110059541A1
公开(公告)日：2011-03-10
申请号：US12672025
申请日：2008-07-14
申请人： Tomoteru Abe
发明人： Tomoteru Abe
IPC分类号： G01N33/48
CPC分类号： G01N33/542 , C12Q1/6818 , Y10T436/143333 , C12Q2563/173
摘要： Provided is a method for obtaining information on the formation of a double-stranded nucleic acid by detecting fluorescence, which is simple, is applicable to various interaction systems, and features high detection sensitivity with reduced background signals. Specifically provided is a method for obtaining information on the formation of a double-stranded nucleic acid, which includes detecting information on fluorescence of a probe consisted of a labeling fluorescent dye labeled on a nucleic acid strand and an intercalator bound or inserted between base pairs in the double-stranded nucleic acid to permit an energy transfer with the fluorescent dye.
摘要翻译：本发明提供一种通过检测荧光获得双链核酸形成信息的方法，该方法简单，适用于各种相互作用系统，并且具有较低的背景信号检测灵敏度。具体提供的是获得关于双链核酸形成的信息的方法，其包括检测由在核酸链上标记的标记荧光染料和嵌入或插入碱基对之间的插入剂的探针的荧光信息双链核酸以允许与荧光染料的能量转移。

19. 发明申请

US20090208931A1 Gene Expression Level Normalization Method, Program and System 审中-公开
标题翻译：基因表达水平归一化方法，程序和系统
公开(公告)号：US20090208931A1
公开(公告)日：2009-08-20
申请号：US11719199
申请日：2005-10-17
申请人： Yasunori Ohto , Tomoteru Abe
发明人： Yasunori Ohto , Tomoteru Abe
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6837 , C12Q2600/158 , G16B25/00 , G16B40/00 , C12Q2545/101 , C12Q2537/143
摘要： It is an object to improve the reliability and accuracy of normalization of gene expression levels, which have been measured separately, by providing a method for the acquisition of an index of high reliability and high accuracy as an index for performing the normalization such that the gene expression levels can be compared and verified. Provided is a method for normalizing a gene expression level, which has been measured for an analysis of a gene expression, by using plural gene expression levels measured for an acquisition of an index. The method includes acquiring a correlation among the plural gene expression levels measured for the acquisition of the index, and using the thus-acquired correlation as an index for normalizing the gene expression level measured for the analysis of the gene expression. The correlation function can be obtained by using, as function values, correlations among plural gene expression levels under at least two sets of experimental conditions (numerals 34 to 38), respectively, with respect to the plural gene expression levels gn acquired under the at least two sets of experimental conditions from cells 31 collected under the at least two sets of experimental conditions, respectively, and selecting a combination of plural ones of the function values such that the plural function values each approximates to a constant value.
摘要翻译：本发明的目的是通过提供以高可靠性和高精度的指标获取的方法作为进行标准化的指标，提高基因表达水平的归一化的可靠性和准确性，这些已被单独测定，使得基因可以比较和验证表达水平。提供了通过使用针对获取指标测量的多个基因表达水平来对基因表达水平进行归一化的方法，其已经被测量用于基因表达的分析。所述方法包括获取针对所述指标的获取而测定的多个基因表达水平之间的相关性，并且使用如此获得的相关性作为用于归一化用于分析所述基因表达的基因表达水平的指标。关于在至少两个实验条件（数字34〜38）下的多个基因表达水平之间的多个基因表达水平之间的相关性，可以分别使用至少在以下获得的多个基因表达水平gn而获得相关函数作为功能值分别在至少两组实验条件下收集的来自单元31的两组实验条件，并且选择多个函数值的组合，使得多个函数值各自近似于常数值。

20. 发明申请

US20100304395A1 Method, Program, and System for Normalizing Gene Expression Amounts 审中-公开
标题翻译：方法，程序和使基因表达量归一化的系统
公开(公告)号：US20100304395A1
公开(公告)日：2010-12-02
申请号：US12854003
申请日：2010-08-10
申请人： Yasunori OHTO , Tomoteru Abe
发明人： Yasunori OHTO , Tomoteru Abe
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q1/68 , G16B25/00 , G16B30/00 , C12Q2545/113
摘要： The present invention aims at presenting novel means for analyzing and correcting gene expression amounts. There is provided a gene expression amount normalizing method in which the number of cells in a sample is obtained by measuring a repeated sequence present in a substantially fixed proportion in a genome contained in the sample, and the number of cells obtained is used as an index for normalizing gene expression amounts obtained from the same sample. For example, a DNA sample 33 and an RNA sample 34 are obtained from the same sample 32, the DNA sample 33 is used as a sample for obtaining the number of cells, and the RNA sample 34 is used as a sample for obtaining the gene expression amounts, whereby the number of cells contained in the sample 32 and the gene expression amounts relating to the same sample 32 can be obtained. Therefore, by converting the gene expression amounts to values per a fixed number of cells, the gene expression amounts can be normalized to values which can be compared with those obtained by other gene expression analyses.
摘要翻译：本发明旨在提出用于分析和校正基因表达量的新手段。提供了一种基因表达量标准化方法，其中通过测量在样品中包含的基因组中基本上固定的比例存在的重复序列获得样品中的细胞数，将获得的细胞数用作指数用于归一化从相同样品获得的基因表达量。例如，从同一样品32获得DNA样品33和RNA样品34，将DNA样品33用作获得细胞数的样品，将RNA样品34用作获得基因的样品表达量，从而可以获得样品32中包含的细胞数和与相同样品32相关的基因表达量。因此，通过将基因表达量转换成固定数目的细胞数，可以将基因表达量归一化为与通过其他基因表达分析获得的那些值相比较的值。

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