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    • 12. 发明申请
    • METHOD FOR FORMATION OF A STATIONARY PHASE IN AN IMMUNOADSORPTION WALL
    • 在免疫吸收壁中形成静止相的方法
    • US20080009079A1
    • 2008-01-10
    • US11456051
    • 2006-07-06
    • Tsung-Hua Yang
    • Tsung-Hua Yang
    • G01N33/543
    • G01N33/5436
    • A method for formation of a stationary phase in an immunoadsorption wall is provided. This method is a partially incomplete two-stage polymerization method and the resulting stationary phase consists of a supporting gel layer and a stacking gel layer. The method is mainly characterized by the change in the temperature and non-uniform concentration distribution of monomer acrylamide in the polymerization reaction system to produce the stationary phase superficially embedded with the immunoadsorbents. Three different ways are introduced to the formation of such a stationary phase including the pre-coupling process, the post-coupling process and the serial copolymerization process. The results of binding activity tests shows that the middle molecular toxin, beta-2-microglobulin, can be removed by the prepared stationary phases.
    • 提供了在免疫吸附壁中形成固定相的方法。 该方法是部分不完全的两阶段聚合方法,所得固定相由支持凝胶层和堆叠凝胶层组成。 该方法的主要特征是聚合反应体系中单体丙烯酰胺的温度变化和浓度分布不均匀,产生了表面嵌入免疫吸附剂的固定相。 引入三种不同的方式来形成这样的固定相,包括预耦合过程,后耦合过程和连续共聚过程。 结合活性测试结果表明,中间分子毒素β-2-微球蛋白可以通过制备的固定相去除。