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11. 发明授权

US08298758B2 Method of multiplex microorganism detection 有权
标题翻译：多重微生物检测方法
公开(公告)号：US08298758B2
公开(公告)日：2012-10-30
申请号：US10584393
申请日：2004-12-24
申请人： Naoko Horikoshi , Susumu Kawasaki , Yukio Okada , Kazuko Takeshita , Takashi Sameshima , Shinichi Kawamoto , Kenji Isshiki
发明人： Naoko Horikoshi , Susumu Kawasaki , Yukio Okada , Kazuko Takeshita , Takashi Sameshima , Shinichi Kawamoto , Kenji Isshiki
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q1/689 , Y02A50/451
摘要： The present invention is to provide a multiple detection method that can detect contaminating microorganisms existing in foods, including pathogenic Escherichia coli O157, Listeria monocytogenes and Salmonella spp., with high sensitivity comparable or even superior to official methods, comprising the steps of amplifying a plural number of target genes with a single PCR reaction tube and analyzing the same. The following steps are performed consecutively: (A) a step of extracting DNA of the target microorganisms to be detected by treating with at least a lytic enzyme such as Achromopepidase and Lysozyme and/or bacteriocin having lytic activity such as Enterolysine, a surfactant and a protein denaturing agent; and (B) a step of mixing a specific primer to the target microorganisms to be detected to perform multiplex PCR. Further, it is preferable to add a step of culturing with a culture condition where 1 CFU/100 g microorganisms becomes 10.sup.3 CFU/ml or more after 18 to 48 h of culture, for example that the pH after culture becomes 5.1 or more, before the step of extracting DNA of the target microorganisms to be detected.
摘要翻译：本发明提供一种可以检测存在于食品中的污染微生物的多重检测方法，包括致病性大肠杆菌O157，单核细胞增生李斯特氏菌和沙门氏菌，具有与官方方法相当或甚至优于其他方法的高灵敏度，包括以下步骤：使用单个PCR反应管的目标基因数目并进行分析。连续进行以下步骤：（A）通过用至少一种裂解酶如溶酶酶和溶菌酶和/或具有溶解活性的细菌素（例如肠溶素，表面活性剂和表面活性剂）进行处理来提取要检测的目标微生物的DNA的步骤蛋白变性剂; 和（B）将特异性引物与要检测的目标微生物混合以进行多重PCR的步骤。此外，优选在培养18〜48小时后，添加培养条件，其中1CFU / 100g微生物变成10μFCFU / ml以上，例如培养后的pH变为5.1 或更多，在提取要检测的目标微生物的DNA的步骤之前。

12. 发明授权

US07472541B2 Compressor control unit and gas turbine power plant including this unit 有权
标题翻译：包括本机组的压缩机控制单元和燃气轮机发电厂
公开(公告)号：US07472541B2
公开(公告)日：2009-01-06
申请号：US11267165
申请日：2005-11-07
申请人： Kazuhiro Takeda , Kazuko Takeshita , Makoto Tsutsui , Hiroaki Yoshida , Kengo Hirano
发明人： Kazuhiro Takeda , Kazuko Takeshita , Makoto Tsutsui , Hiroaki Yoshida , Kengo Hirano
IPC分类号： F02C3/22 , F02C7/00
CPC分类号： F04D27/0207 , F04D27/02 , F04D27/0246
摘要： Provided is a compressor control unit with a high response ability to changes of gas condition of a compressor (compressor suction temperature, pressure, gas specific gravity, pressure ratio of suction pressure and discharge pressure). In the control unit for a compressor that supplies gas into a gas turbine through a header tank, an inlet gas condition is measured and a load command value from a gas turbine controller is corrected to be increased or decreased corresponding to the measured inlet gas condition.
摘要翻译：提供了一种对压缩机的气体状态变化（压缩机吸入温度，压力，气体比重，吸入压力和排出压力的压力比）具有高响应能力的压缩机控制单元。在用于通过集水箱向气体涡轮机供给气体的压缩机的控制单元中，测定入口气体状况，根据测定的入口气体条件，将来自燃气轮机控制器的负荷指令值修正为增减。

13. 发明申请

US20060101824A1 Compressor control unit and gas turbine power plant including this unit 有权
公开(公告)号：US20060101824A1
公开(公告)日：2006-05-18
申请号：US11267165
申请日：2005-11-07
申请人： Kazuhiro Takeda , Kazuko Takeshita , Makoto Tsutsui , Hiroaki Yoshida , Kengo Hirano
发明人： Kazuhiro Takeda , Kazuko Takeshita , Makoto Tsutsui , Hiroaki Yoshida , Kengo Hirano
IPC分类号： F02C9/16
CPC分类号： F04D27/0207 , F04D27/02 , F04D27/0246
摘要： Provided is a compressor control unit with a high response ability to changes of gas condition of a compressor (compressor suction temperature, pressure, gas specific gravity, pressure ratio of suction pressure and discharge pressure). In the control unit for a compressor that supplies gas into a gas turbine through a header tank, an inlet gas condition is measured and a load command value from a gas turbine controller is corrected to be increased or decreased corresponding to the measured inlet gas condition.

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