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    • 13. 发明授权
    • Bacterial membrane protein secretion
    • 细菌膜蛋白分泌
    • US08450086B2
    • 2013-05-28
    • US13509287
    • 2010-11-15
    • Chichi HuangLu Lu
    • Chichi HuangLu Lu
    • A61K39/42C12P21/00
    • C07K14/395C07K2319/03
    • Improved bacterial secretion signals derived from pelB and ompA are provided. The improved variants enhance bacterial membrane secretion are thus useful for production of proteins secreted from bacteria including proteins displayed on filamentous phage particles, and, in particular, proteins requiring oxidative formation of covalent bonds, such as disulfide bonds within or between polypeptide chains in order to form a correctly folded and functional protein structure. Described herein are methods for the multivalent display of complex dimeric proteins on the surface of a bacteriophage particle and combinatorial synthetic libraries of such proteins displayed as a fusion polypeptide with filamentous phage pIX coat protein. Heterodimeric or more complex interchain bonded structures may also be displayed using the method of the invention.
    • 提供了从pelB和ompA得到的改善的细菌分泌信号。 因此,改进的变体增强细菌膜分泌因此可用于产生从细菌分泌的蛋白质,包括显示在丝状噬菌体颗粒上的蛋白质,特别是需要氧化形成共价键的蛋白质,例如多肽链内或多肽链之间的二硫键,以便 形成正确折叠和功能的蛋白质结构。 本文描述的是在噬菌体颗粒的表面上复杂二聚体蛋白质的多价显示和显示为具有丝状噬菌体pIX外壳蛋白的融合多肽的蛋白质的组合合成文库的方法。 也可以使用本发明的方法显示异二聚体或更复杂的链间结合结构。