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11. 发明授权

US06906238B2 Effective nuclear reprogramming in mammals using CDK2 inhibitors 失效
标题翻译：使用CDK2抑制剂对哺乳动物进行有效的核重编程
公开(公告)号：US06906238B2
公开(公告)日：2005-06-14
申请号：US09809662
申请日：2001-03-15
申请人： Steven Stice
发明人： Steven Stice
IPC分类号： C12N15/873 , C12N15/00 , A01K67/027 , C12N5/02
CPC分类号： C12N15/873
摘要： The present invention provides methods of producing a cloned non-human mammalian nuclear transfer (NT) embryo and methods for producing a cloned non-human mammal. Embodiments of the methods include introducing donor genetic material into a metaphase I oocyte; introducing donor genetic material into a non-enucleated oocyte; introducing donor genetic material obtained from a donor cell that is at metaphase into an oocyte; introducing donor genetic material into an oocyte, and naturally activating the oocyte or the NT embryo; and introducing donor genetic material obtained from a donor cell that is at late G1 phase into an oocyte.
摘要翻译：本发明提供了产生克隆的非人哺乳动物核转移（NT）胚胎的方法和用于产生克隆的非人哺乳动物的方法。方法的实施方案包括将供体遗传物质引入中期卵母细胞; 将供体遗传物质引入非去核卵母细胞; 将从中期的供体细胞获得的供体遗传物质引入卵母细胞; 将供体遗传物质引入卵母细胞，并自然激活卵母细胞或NT胚胎; 并将从晚期G1期的供体细胞获得的供体遗传物质引入卵母细胞。

12. 发明申请

US20120076761A1 Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including Parkinson's disease 审中-公开
标题翻译：克隆有蹄类动物的胚胎和动物，使用细胞，组织和器官进行移植治疗，包括帕金森病
公开(公告)号：US20120076761A1
公开(公告)日：2012-03-29
申请号：US12896396
申请日：2010-10-01
申请人： Steven Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce De Leon , D. Joseph Jerry
发明人： Steven Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce De Leon , D. Joseph Jerry
IPC分类号： A61K35/30 , C12N5/0793 , A61P25/16 , A61P37/06 , C12N5/079 , C12N5/10
CPC分类号： A01K67/02 , A01K67/027 , A01K67/0271 , A01K67/0275 , A01K2217/05 , A01K2227/101 , A61K35/12 , A61K35/30 , A61K38/00 , C12N5/0603 , C12N15/8771 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： Methods and cell lines for cloning ungulate embryos and offspring, in particular bovines and porcines, are provided. The resultant fetuses, embryos or offspring are especially useful for the expression of desired heterologous DNAs, and may be used as a source of cells or tissue for transplantation therapy for the treatment of diseases such as Parkinson's disease.
摘要翻译：提供用于克隆有蹄类胚胎和后代的方法和细胞系，特别是牛和猪。所产生的胎儿，胚胎或后代对于所需的异源DNA的表达特别有用，并且可以用作用于治疗诸如帕金森病之类疾病的移植治疗的细胞或组织来源。

13. 发明申请

US20110044954A1 Methods of producing germ-like cells and related therapies 审中-公开
标题翻译：生产类细胞的方法及相关疗法
公开(公告)号：US20110044954A1
公开(公告)日：2011-02-24
申请号：US12583402
申请日：2009-08-20
申请人： Steven Stice , Franklin West
发明人： Steven Stice , Franklin West
IPC分类号： A61K35/12 , C12N5/071 , C12N5/07 , C12N5/00 , A61P15/00
CPC分类号： C12N5/0611 , A61K35/48 , C12N2501/115 , C12N2501/155 , C12N2502/13 , C12N2506/02 , C12N2506/45 , C12N2533/90
摘要： The present invention relates to methods of producing germ-like cells (GLCs) from embryonic stem cells and induced pluripotent stem cells, GLCs produced by such methods, gametes derived from such GLCs, pharmaceutical compositions and kits containing such GLCs, screens that use GLCs to identify agents useful in enhancing mammalian reproductive health, and methods of treatment that use GLCs to enhance mammalian reproductive health.
摘要翻译：本发明涉及从胚胎干细胞和诱导的多能干细胞产生胚状细胞（GLC）的方法，通过这种方法产生的GLC，源自这种GLC的配子，药物组合物和含有这种GLC的试剂盒，使用GLC的筛选鉴定有助于增强哺乳动物生殖健康的药物，以及使用GLCs增强哺乳动物生殖健康的治疗方法。

14. 发明申请

US20090092588A1 Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including parkinson's disease 审中-公开
标题翻译：克隆有蹄类动物的胚胎和动物，使用细胞，组织和器官进行移植治疗，包括帕金森病
公开(公告)号：US20090092588A1
公开(公告)日：2009-04-09
申请号：US11529720
申请日：2006-09-26
申请人： Steven Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce De Leon , D. Joseph Jerry
发明人： Steven Stice , Jose Cibelli , James Robl , Paul Golueke , F. Abel Ponce De Leon , D. Joseph Jerry
IPC分类号： A61K35/12 , C12N15/87 , C12N5/08
CPC分类号： A01K67/02 , A01K67/027 , A01K67/0271 , A01K67/0273 , A01K67/0275 , A01K2217/05 , A01K2227/101 , A61K35/12 , A61K35/30 , A61K38/00 , C12N5/0603 , C12N15/8771 , C12N15/8778 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： Methods and cell lines for cloning ungulate embryos and offspring, in particular bovines and porcines, are provided. The resultant fetuses, embryos or offspring are especially useful for the expression of desired heterologous DNAs, and may be used as a source of cells or tissue for transplantation therapy for the treatment of diseases such as Parkinson's disease.
摘要翻译：提供用于克隆有蹄类胚胎和后代的方法和细胞系，特别是牛和猪。所产生的胎儿，胚胎或后代对于所需的异源DNA的表达特别有用，并且可以用作用于治疗诸如帕金森病之类疾病的移植治疗的细胞或组织来源。

15. 发明申请

US20080274446A1 Cryopreserved human neuronal cultures 审中-公开
标题翻译：冷冻保存人类神经元培养物
公开(公告)号：US20080274446A1
公开(公告)日：2008-11-06
申请号：US12150818
申请日：2008-05-01
申请人： Steven Stice , David Machacek
发明人： Steven Stice , David Machacek
IPC分类号： A01N1/02 , C12N5/08
CPC分类号： A01N1/02 , A01N1/0231 , C12N5/0619 , C12N2501/115 , C12N2501/235 , C12N2502/13 , C12N2506/02 , C12N2533/52
摘要： Cryopreserved cultures of post-mitotic neuronal or neural-like cells are provided having a viability after thaw of greater than 10%, typically greater than 50%. Once thawed, the cells are capable of further differentiation. In one embodiment, less than 20% of the cryopreserved cells are self-proliferating cells. The cells can be provided in a kit including a container of the cryopreserved neuronal or neural-like cells, optionally including additional cell culture reagents and materials. Method for preparing the cryopreserved neuronal or neural-like cells derived from embryonic stem cells, preferably human embryonic stem cells, are also provided.
摘要翻译：提供后有丝分裂神经元或神经样细胞的冷冻保存培养物，其解冻后的存活力大于10％，通常大于50％。解冻后，细胞能够进一步分化。在一个实施方案中，低于20％的冷冻保存细胞是自增殖细胞。细胞可以提供在包括冷冻保存的神经元或神经样细胞的容器的试剂盒中，任选地包括另外的细胞培养试剂和材料。还提供了用于制备源自胚胎干细胞，优选人胚胎干细胞的冷冻保存的神经元或神经样细胞的方法。

16. 发明申请

US20050095704A1 Embryonic or stem-like cell lines produced by cross species nuclear transplantation 审中-公开
标题翻译：通过异种核移植产生的胚胎或干细胞系
公开(公告)号：US20050095704A1
公开(公告)日：2005-05-05
申请号：US10922374
申请日：2004-08-25
申请人： James Robl , Jose Cibelli , Steven Stice
发明人： James Robl , Jose Cibelli , Steven Stice
IPC分类号： A61K35/12 , A61K38/00 , A61K48/00 , C12N5/0735 , C12N15/87 , C12N15/873 , C12N15/877 , C12N5/08
CPC分类号： C12N15/873 , A61K35/12 , A61K38/00 , A61K48/00 , C12N5/0606 , C12N15/87 , C12N15/877 , C12N2510/00 , C12N2517/04
摘要： An improved method of nuclear transfer involving the transplantation of donor cell nuclei into enucleated oocytes of a species different from the donor cell is provided. The resultant nuclear transfer units are useful for the production of isogenic embryonic stem cells, in particular human isogenic embryonic or stem cells. These embryonic or stem-like cells are useful for producing desired differentiated cells and for introduction, removal or modification, of desired genes, e.g., at specific sites of the genome of such cells by homologous recombination. These cells, which may contain a heterologous gene, are especially useful in cell transplantation therapies and for in vitro study of cell differentiation.
摘要翻译：提供了涉及将供体细胞核移植到与供体细胞不同的物种的去核卵母细胞中的改进的核转移方法。所得到的核转移单位可用于生产等原胚胎干细胞，特别是人原代胚胎干细胞。这些胚胎或干细胞样细胞可用于产生所需的分化细胞并用于通过同源重组引入，去除或修饰所需基因，例如在这些细胞的基因组的特定位点。这些可能含有异源基因的细胞特别适用于细胞移植治疗和体外研究细胞分化。

17. 发明申请

US20050074439A1 Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including Parkinson's disease 审中-公开
标题翻译：克隆有蹄类动物的胚胎和动物，使用细胞，组织和器官进行移植治疗，包括帕金森病
公开(公告)号：US20050074439A1
公开(公告)日：2005-04-07
申请号：US10818486
申请日：2004-04-05
申请人： Steven Stice , Jose Cibelli , James Robl
发明人： Steven Stice , Jose Cibelli , James Robl
IPC分类号： A01K67/02 , A01K67/027 , A61K35/12 , A61K38/00 , C12N5/073 , C12N15/877 , A61K48/00
CPC分类号： A01K67/0275 , A01K67/02 , A01K67/027 , A01K67/0273 , A01K2217/05 , A01K2227/101 , A61K35/12 , A61K38/00 , C12N5/0603 , C12N15/8771 , C12N15/8778 , C12N2510/00 , C12N2517/02 , C12N2517/04
摘要： Methods and cell lines for cloning ungulate embryos and offspring, in particular bovines and porcines, are provided. The resultant fetuses, embryos or offspring are especially useful for the expression of desired heterologous DNAs, and may be used as a source of cells or tissue for transplantation therapy for the treatment of diseases such as Parkinson's disease.
摘要翻译：提供用于克隆有蹄类胚胎和后代的方法和细胞系，特别是牛和猪。所产生的胎儿，胚胎或后代对于所需的异源DNA的表达特别有用，并且可以用作用于治疗诸如帕金森病之类疾病的移植治疗的细胞或组织来源。

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