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11. 发明授权

US08908174B2 Apparatus, especially microscope, for the analysis of samples 有权
标题翻译：仪器，特别是显微镜，用于分析样品
公开(公告)号：US08908174B2
公开(公告)日：2014-12-09
申请号：US13121919
申请日：2009-09-22
申请人： Michael Kempe , Gerhard Krampert , Ingo Kleppe , Ralf Wolleschensky
发明人： Michael Kempe , Gerhard Krampert , Ingo Kleppe , Ralf Wolleschensky
IPC分类号： G01J3/28 , G02B21/16 , G02B21/00 , G01N21/64
CPC分类号： G01N21/6428 , G01N21/6458 , G01N2021/6417 , G02B21/0076 , G02B21/16
摘要： A microscope device which has a diffraction-limited resolution volume, with multiple dye molecules that can be switched between different states, at least one of which is fluorescent. The fluorescence is focused using an objective lens and is imaged onto a spatially resolving detector. In at least one portion of the sample, the dye molecules have a distribution density that is greater than the inverse of the diffraction-limited resolution volume. One or more light sources are provided for emitting a switching radiation in order to switch a first subset of the dye molecules in the sample, and for emitting an excitation radiation in order to excite the first subset of dye molecules. A phase mask which generates a light distribution having an at least partially limited local minimum radiation on the detector plane is provided in the beam path, preferably in the detection beam path.
摘要翻译：具有衍射限制分辨率体积的显微镜装置，具有可以在不同状态之间切换的多个染料分子，其中至少一个是荧光的。使用物镜聚焦荧光，并将其成像到空间分辨检测器上。在样品的至少一部分中，染料分子的分布密度大于衍射极限分辨率体积的倒数。提供一个或多个光源用于发射切换辐射，以便切换样品中的染料分子的第一子集，并且用于发射激发辐射以激发染料分子的第一子集。优选在检测光束路径中，在光束路径中设置产生具有至少部分受限局部最小辐射的光分布的相位掩模。

12. 发明授权

US08728062B2 Device and method for controlling a laser therapy of the eye 有权
标题翻译：用于控制眼睛激光治疗的装置和方法
公开(公告)号：US08728062B2
公开(公告)日：2014-05-20
申请号：US13070217
申请日：2011-03-23
申请人： Manfred Dick , Martin Hacker , Michael Kempe
发明人： Manfred Dick , Martin Hacker , Michael Kempe
IPC分类号： A61B18/20 , A61B3/10 , A61B5/15 , A61B5/00
CPC分类号： A61B3/102 , A61B5/0093 , A61B5/15 , A61F9/00821 , A61F2009/00851 , A61F2009/00863 , G01B9/02091
摘要： A device for controlling a laser therapy of the eye, including an evaluation unit that determines an intensity of a transient temperature effect by analysis of interferometric signals obtained from the eye and a control unit that controls the laser therapy, which is based on said transient temperature effect, the control unit being connected with said evaluation unit. A method for controlling a laser therapy of the eye, includes determination of the intensity of a transient temperature effect that is utilized for the control of the laser therapy, based on the effect, by analysis of interferometric signals.
摘要翻译：一种用于控制眼睛的激光治疗的装置，包括通过分析从眼睛获得的干涉信号和控制激光治疗的控制单元来确定瞬时温度效应的强度的评估单元，其基于所述瞬态温度所述控制单元与所述评估单元相连。用于控制眼睛的激光治疗的方法包括通过分析干涉信号来确定用于激光治疗的控制的瞬时温度效应的强度。

13. 发明授权

US08704196B2 Combination microscopy 有权
标题翻译：组合显微镜
公开(公告)号：US08704196B2
公开(公告)日：2014-04-22
申请号：US13127427
申请日：2009-10-28
申请人： Ralf Wolleschensky , Ingo Kleppe , Gerhard Krampert , Michael Kempe
发明人： Ralf Wolleschensky , Ingo Kleppe , Gerhard Krampert , Michael Kempe
IPC分类号： G01J1/58
CPC分类号： G02B27/642 , G01N21/6458 , G02B21/0076 , G02B21/367 , G02B27/58
摘要： A method for generating an image of a sample by a microscopy method including varying local resolution, wherein at least two of the following microscopy methods are combined: laser scanning microscopy, a microscopy method wherein the sample is excited to luminescence by structured line or wide area illumination, and a first microscopy image is generated from the images thus obtained, having increased local resolution greater than the optical resolution of the image, a further microscopy method according to the PAL principle, by which a second microscopy image is generated, indicating geometric locations of marker molecules emitting luminescent radiation at an increased local resolution relative to the optical resolution, and a further microscopy method, wherein the sample is marked using marking molecules suitable for the STED, ESA, or RESOLFT technique, and a third microscopy image is generated of STED, ESA, or RESOLFT, wherein the obtained images are superimposed.
摘要翻译：一种用于通过包括变化的局部分辨率的显微镜方法产生样品的图像的方法，其中组合以下显微镜方法中的至少两种：激光扫描显微镜，其中通过结构化线或广泛区域将样品激发至发光的显微镜方法照明，并且从由此获得的图像产生第一显微镜图像，其具有大于图像的光学分辨率的增加的局部分辨率，根据PAL原理的另一显微镜方法，通过该方法生成第二显微镜图像，指示几何位置相对于光学分辨率以增加的局部分辨率发射发光辐射的标记分子，以及另外的显微镜方法，其中使用适合于STED，ESA或RESOLFT技术的标记分子标记样品，并且产生第三显微镜图像 STED，ESA或RESOLFT，其中所获得的图像被叠加。

14. 发明授权

US08610086B2 Increased resolution microscopy 有权
标题翻译：增加分辨率显微镜
公开(公告)号：US08610086B2
公开(公告)日：2013-12-17
申请号：US13131801
申请日：2009-11-14
申请人： Ralf Wolleschensky , Ingo Kleppe , Gerhard Krampert , Michael Kempe
发明人： Ralf Wolleschensky , Ingo Kleppe , Gerhard Krampert , Michael Kempe
IPC分类号： G01N21/64
CPC分类号： G02B21/16 , G01N21/6458 , G02B21/361 , G02B21/367 , G02B27/58
摘要： Method for spatially high-resolution luminescence microscopy in which label molecules in a sample are activated to emit luminescence radiation comprising activating only a subset of the label molecules in the sample, wherein activated label molecules have a distance to the closest activated molecules that is greater or equal to a length which results from a predetermined optical resolution, detecting the luminescence radiation, generating a frame from the luminescence radiation, identifying the geometric locations of the label molecules with a spatial resolution increased above the predetermined optical resolution, repeating the steps and forming a combined image, and controlling the acquisition of the several frames by evaluating at least one of the frames or a group of the frames and modifying at least one variable for subsequent repetitions of the steps of generating frames for combining into an image.
摘要翻译：用于空间高分辨率发光显微镜的方法，其中样品中的标记分子被激活以发射发光，包括仅激活样品中标记分子的一个子集，其中激活的标记分子与最接近的激活分子的距离更大或等于由预定的光学分辨率产生的长度，检测发光辐射，从发光辐射产生帧，以高于预定的光学分辨率的空间分辨率识别标签分子的几何位置，重复步骤并形成并且通过评估所述帧或一组帧中的至少一个并且修改至少一个变量来控制所述几帧的获取，以便后续重复生成用于组合成图像的帧的步骤。

15. 发明授权

US08570625B2 Device and method for generating an image of an object 有权
标题翻译：用于生成对象的图像的装置和方法
公开(公告)号：US08570625B2
公开(公告)日：2013-10-29
申请号：US12088557
申请日：2006-09-14
申请人： Michael Kempe , Ralf Wolleschensky
发明人： Michael Kempe , Ralf Wolleschensky
IPC分类号： H04N1/46 , H04N1/04
CPC分类号： G02B21/0032 , G02B21/006
摘要： A device for generating an image of an object is provided, comprising an illumination module, by means of which the object can be illuminated with a pattern whose phase is altered temporally, a recording module, by means of which a plurality of recordings of the object are carried out during the phase change of the pattern, and a processing module, which generates the image from the recordings, wherein the illumination module moves a light beam over the object and modulates its intensity synchronously with the movement such that the beam generates the pattern in scanning fashion.
摘要翻译：提供了一种用于产生物体的图像的装置，包括照明模块，利用该照明模块可以利用其相位在时间上改变的图案来照亮物体;记录模块，借助于该物体的多个记录在图案的相变期间进行，以及处理模块，其从记录产生图像，其中照明模块将光束移动到物体上并且与移动同步地调制其强度，使得光束产生图案在扫描时尚。

16. 发明授权

US08389893B2 Laser beam machining 有权
标题翻译：激光束加工
公开(公告)号：US08389893B2
公开(公告)日：2013-03-05
申请号：US12744216
申请日：2008-11-20
申请人： Michael Kempe , Peter Westphal , Wolfgang Grau , Georg von Freymann
发明人： Michael Kempe , Peter Westphal , Wolfgang Grau , Georg von Freymann
IPC分类号： B23K26/00 , B23K26/14 , B23K26/16 , B23K26/06 , B23K26/02 , B23K26/08 , G02B21/00
CPC分类号： B23K26/046 , G02B7/32 , G02B21/244 , G02B21/245 , G03F7/70375 , G03F7/70383
摘要： A method for laser beam machining of a workpiece in which a laser beam is focused by an objective, into or onto the workpiece having a boundary surface, to produce a machining effect by a two-photon process, and the position of the focal point with respect to the workpiece is shifted. To obtain a reference for the position of the focal point, an image of a luminating modulation object is projected through the objective onto the workpiece into the focal plane or so as to intersect it. Reflections of the image occurring at the boundary surface are imaged into an autofocus image plane, and are detected by a camera. The camera image plane either intersects the autofocus image plane when the image of the illuminating modulation object lies in the focal plane, or lies in the autofocus image plane when the image of the modulation object intersects the focal plane.
摘要翻译：一种用于激光束加工的方法，其中激光束被物镜聚焦到具有边界表面的工件中或之上，以通过双光子过程产生加工效果，并且焦点的位置与相对于工件移动。为了获得焦点的位置的参考，发光调制对象的图像通过物镜投射到工件上进入焦平面或与之相交。在边界面发生的图像的反射被成像为自动聚焦图像平面，并且由相机检测。当照明调制对象的图像位于焦平面时，相机图像平面与自动聚焦图像平面相交，或者当调制对象的图像与焦平面相交时，相机图像平面位于自动聚焦图像平面中。

17. 发明授权

US08280131B2 Method and configuration for optically detecting an illuminated specimen 有权
标题翻译：用于光学检测被照射样品的方法和结构
公开(公告)号：US08280131B2
公开(公告)日：2012-10-02
申请号：US12323123
申请日：2008-11-25
申请人： Michael Kempe , Ralf Wolleschensky
发明人： Michael Kempe , Ralf Wolleschensky
IPC分类号： G06K9/00 , G01J1/42 , G02B21/00
CPC分类号： G02B21/0032 , G02B21/0048 , G02B21/0056
摘要： A configuration for the optical detection of a specimen, wherein the specimen or at least part of the specimen is scanned by means of linear illumination by scanning means, means for linear beam shaping of the illuminating light are provided, and the illuminating light has a preferably periodic structure in at least one spatial direction in that means for generating the structure are disposed in the illuminating beam path, light coming from the specimen is detected and images of the specimen are generated therefrom, at least one optical sectional image through the specimen and/or one image with increased resolution is/are calculated from the images, and means for generating the structure are disposed downstream of the scanning means in the direction of the illumination.
摘要翻译：提供了一种用于通过扫描装置通过线性照明扫描样本或样本的至少一部分的样本的光学检测的结构，用于照明光的线性光束成形的装置，并且照明光具有优选的在所述照明光束路径中设置用于产生所述结构的装置中的至少一个空间方向上的周期性结构，并且检测来自所述样本的光并且从其产生所述样本的图像，通过所述样本的至少一个光学截面图像和/ 或者从图像计算一个具有增加的分辨率的图像，并且用于产生该结构的装置设置在扫描装置的照明方向的下游。

18. 发明申请

US20110174986A1 Apparatus, Especially Microscope, for the Analysis of Samples 有权
标题翻译：仪器，特别是显微镜，用于样品分析
公开(公告)号：US20110174986A1
公开(公告)日：2011-07-21
申请号：US13121919
申请日：2009-09-22
申请人： Michael Kempe , Gerhard Krampert
发明人： Michael Kempe , Gerhard Krampert
IPC分类号： G01N21/64
CPC分类号： G01N21/6428 , G01N21/6458 , G01N2021/6417 , G02B21/0076 , G02B21/16
摘要： Disclosed is an apparatus, especially a microscope, characterized by a diffraction-limited resolution volume, comprising multiple dye molecules (UF) that can be switched between different states, at least one of which is fluorescent. The fluorescence is focused using an objective lens (O) and is imaged onto a spatially resolving detector. In at least one portion of the sample, the UF have a distribution density that is greater than the inverse of the diffraction-limited resolution volume. Said apparatus further comprises one or more light sources for emitting a switching radiation in order to switch a first subset of the UF in the sample, and for emitting an excitation radiation in order to excite the first subset of UF. A phase mask which generates a light distribution (PSF) having an at least partially limited local minimum radiation on the detector plane is provided in the beam path, preferably in the detection beam path. Alternatively, an axicon is provided for generating a Bessel distribution (PSF) on the detector plane, a means for structuring the illumination distribution is provided in the illumination beam path, means for spectrally splitting the sample light are provided in the detection beam path, a receiver array composed of position-sensitive receivers is provided for detection purposes, means causing a color-dependent light distribution (PSF) on the detector plane are provided in the detection beam path, or an achromatic beam splitter is arranged in or near the pupil.
摘要翻译：公开了一种特征在于衍射限制分辨率体积的装置，特别是显微镜，包括可以在不同状态之间切换的多个染料分子（UF），其中至少一个是荧光的。使用物镜（O）聚焦荧光，并将其成像到空间分辨检测器上。在样品的至少一部分中，UF的分布密度大于衍射极限分辨率体积的倒数。所述装置还包括用于发射切换辐射的一个或多个光源，以便切换样本中的UF的第一子集，并且用于发射激发辐射以便激发UF的第一子集。优选地在检测光束路径中，在光束路径中设置产生在检测器平面上具有至少部分受限局部最小辐射的光分布（PSF）的相位掩模。或者，提供了用于在检测器平面上产生贝塞尔分布（PSF）的旋转三棱镜，在照明光束路径中提供用于构造照明分布的装置，在检测光束路径中提供用于频谱分割样本光的装置，提供了由位置敏感接收器组成的接收器阵列用于检测目的，在检测器光路中提供在检测器平面上引起依赖于颜色的光分布（PSF）的装置，或者在瞳孔中或附近设置消色差分束器。

19. 发明授权

US07633053B2 Microscope, particularly a laser scanning microscope with adaptive optical arrangement 有权
标题翻译：显微镜，特别是具有自适应光学布置的激光扫描显微镜
公开(公告)号：US07633053B2
公开(公告)日：2009-12-15
申请号：US10461234
申请日：2003-06-13
申请人： Ralf Wolleschensky , Michael Kempe
发明人： Ralf Wolleschensky , Michael Kempe
IPC分类号： H01J3/14 , G02B7/04 , G02B17/00
CPC分类号： G02B21/002 , G02B26/06
摘要： A microscope, particularly a laser scanning microscope, with an adaptive optical device in the microscope beam path, comprising two reflective adaptive elements, at least one of which is constructed as an adaptive optical element, both of which are oriented with their reflector surface vertical to the optical axes of the microscope beam path, and a polarizing beam splitter whose splitter layer is located in the vertex of two orthogonal arms of the microscope beam path or two orthogonal portions of a folded microscope beam path, wherein a first adaptive element is associated with one arm and the other adaptive element is associated with the second arm, and a quarter-wave plate is located in each arm between the beam splitter and reflective adaptive element, and a detection device to which the detection light is directed and which is linked to the adaptive elements by evaluating and adjusting devices.
摘要翻译：在显微镜光路中具有自适应光学装置的显微镜，特别是激光扫描显微镜，包括两个反射自适应元件，其中至少一个被构造为自适应光学元件，它们的反射器表面垂直于显微镜光束路径的光轴和分束器层位于显微镜光束路径的两个正交臂的顶点或折叠显微镜光束路径的两个正交部分的顶点的偏振分束器，其中第一自适应元件与一个臂和另一个自适应元件与第二臂相关联，四分之一波片位于分束器和反射自适应元件之间的每个臂中，以及检测装置，检测光被引导到该检测装置，通过评估和调整设备的自适应元件。

20. 发明申请

US20090128898A1 MICROSCOPY METHOD AND MICROSCOPE 有权
标题翻译：微观方法和显微镜
公开(公告)号：US20090128898A1
公开(公告)日：2009-05-21
申请号：US12088410
申请日：2006-09-14
申请人： Ralf Wolleschensky , Michael Kempe
发明人： Ralf Wolleschensky , Michael Kempe
IPC分类号： G02B21/06
CPC分类号： G02B21/002
摘要： A microscopy method is provided for generating an image of an image field passing in a predetermined depth of a sample to be examined, comprising a plurality of illumination steps, in which a part of the image field is in each case illuminated with a focused illumination beam bundle, which effects the generation of sample radiation on account of an interaction with the sample, detection steps, in which the sample radiation generated is detected, and an evaluation step, in which the image is generated on the basis of the sample radiation detected, wherein a first and second detection step are carried out during each illumination step, wherein sale radiation generated at the focus and outside the focus is detected in the first detection step and a smaller proportion of the sample radiation generated at the focus than in the first detection step and also sample radiation generated outside the focus are detected in the second detection step, and wherein the sample radiation detected in the second detection step is used in the evaluation step to reduce the proportion outside the focus in the sample radiation detected in the first detection step.
摘要翻译：提供了一种显微镜方法，用于产生通过待检查样本的预定深度的图像场的图像，包括多个照明步骤，其中图像场的一部分在每种情况下被照射聚焦照明光束束，其由于与样品的相互作用而影响样品辐射的产生，其中检测到产生的样品辐射的检测步骤以及基于检测到的样品辐射而产生图像的评估步骤，其中在每个照明步骤期间执行第一和第二检测步骤，其中在所述第一检测步骤中检测在所述焦点处和在焦点外产生的销售辐射，以及在所述焦点处产生的样本辐射的比例小于所述第一检测中的比例在第二检测步骤中检测出在焦点外部产生的步进和样本辐射，并且其中检测到的样本辐射在评估步骤中使用第二检测步骤来减少在第一检测步骤中检测到的样品辐射中的焦点外的比例。

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