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11. 发明授权

US08252972B2 Bacillus thuringiensis gene with lepidopteran activity 有权
标题翻译：具有鳞翅目昆虫活性的苏云金芽孢杆菌基因
公开(公告)号：US08252972B2
公开(公告)日：2012-08-28
申请号：US12652780
申请日：2010-01-06
申请人： Andre R. Abad , Hua Dong , Deirdre M. Kapka-Kitzman , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Deirdre M. Kapka-Kitzman , Sue B. Lo , Xiaomei Shi
IPC分类号： C12N15/82 , C12N15/32 , C12N5/10 , C12N1/21 , C12P21/02 , A01H5/00 , A01H5/10
CPC分类号： C07K14/325 , A01N63/02 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编码具有杀虫活性的昆虫的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其包括鳞翅目昆虫害虫。本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，DNA构建体和转化的微生物的分离的核酸和包含实施方案的核酸的植物。这些组合物可用于防治害虫，特别是植物害虫的方法。

12. 发明申请

US20100269221A1 Novel Bacillus Thuringiensis Gene with Lepidopteran Activity 有权
公开(公告)号：US20100269221A1
公开(公告)日：2010-10-21
申请号：US12436171
申请日：2009-05-06
申请人： Andre R. Abad , Hua Dong , Susan B. Lo , Mark Nelson , Xiaomei Shi , Clement W.K. Waine , Takashi Yamamoto
发明人： Andre R. Abad , Hua Dong , Susan B. Lo , Mark Nelson , Xiaomei Shi , Clement W.K. Waine , Takashi Yamamoto
IPC分类号： A01H1/00 , A01H5/00 , C12N15/82 , C07H21/04 , C12N1/21 , C12N5/10 , C12N15/63 , C12N15/79 , C07K14/00 , A01N61/00 , C12P21/02 , A01P7/04
CPC分类号： C12N15/8286 , C07K14/32 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

13. 发明申请

US20090214509A1 Novel Bacillus Thuringiensis Gene with Lepidopteran Activity 审中-公开
公开(公告)号：US20090214509A1
公开(公告)日：2009-08-27
申请号：US12431207
申请日：2009-04-28
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi , Cao Guo Yu
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi , Cao Guo Yu
IPC分类号： A01N37/18 , C12N9/88 , A01P7/00
CPC分类号： C12N15/8286 , C07K14/325 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

14. 发明申请

US20090183276A1 Novel Bacillus Thuringiensis Gene with Coleopteran Activity 审中-公开
公开(公告)号：US20090183276A1
公开(公告)日：2009-07-16
申请号：US12340811
申请日：2008-12-22
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
IPC分类号： C12N15/11 , A01H5/00 , C12N1/21 , C12N5/04 , C07K2/00 , A61K38/02 , C12P21/04
CPC分类号： H04N5/2628
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

15. 发明授权

US07521235B2 Unique novel Bacillus thuringiensis gene with Lepidopteran activity 有权
标题翻译：独特的新型苏云金芽孢杆菌基因与鳞翅目活动
公开(公告)号：US07521235B2
公开(公告)日：2009-04-21
申请号：US11780503
申请日：2007-07-20
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
IPC分类号： C07H19/00 , C07H19/22 , A01H5/00 , C12N15/00 , C12N15/82 , C12N15/87 , C12N5/04 , A01H1/00 , C07K1/00 , C07K14/00 , C07K17/00 , C12N5/00
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编码具有杀虫活性的昆虫的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其包括鳞翅目昆虫害虫。本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，DNA构建体和转化的微生物的分离的核酸和包含实施方案的核酸的植物。这些组合物可用于防治害虫，特别是植物害虫的方法。

16. 发明授权

US07510878B2 Bacillus thuringiensis gene with lepidopteran activity 有权
公开(公告)号：US07510878B2
公开(公告)日：2009-03-31
申请号：US11780511
申请日：2007-07-20
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
IPC分类号： C07H19/00 , C07H19/22 , A01H5/00 , C12N15/00 , C12N15/82 , C12N15/87 , A01H1/00 , C07K1/00 , C07K14/00 , C07K17/00 , C12N1/20 , C12N5/00
CPC分类号： C12N15/8286 , C12Q1/6813 , Y02A40/162 , C12Q2531/113
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

17. 发明申请

US20080026392A1 METHOD FOR IDENTIFYING NOVEL GENES 有权
标题翻译：识别新基因的方法
公开(公告)号：US20080026392A1
公开(公告)日：2008-01-31
申请号：US11780884
申请日：2007-07-20
申请人： Andre Abad , Hua Dong , Susan Lo , Billy McCutchen , Xiaomei Shi
发明人： Andre Abad , Hua Dong , Susan Lo , Billy McCutchen , Xiaomei Shi
IPC分类号： C12Q1/68
CPC分类号： C12N15/8286 , C12Q1/6813 , Y02A40/162 , C12Q2531/113
摘要： Methods and compositions for identifying novel genes that share regions of homology with known genes from target groups of genes of interest are provided. The methods comprise systematically designing oligonucleotide primers that are specific for regions of homology within the nucleotide sequences of a target group of known genes and performing successive rounds of PCR amplification of nucleic acid material from an organism of interest. The PCR steps are intended to identify and amplify nucleic acids comprising both known and novel genes. Nucleic acid molecules comprising known genes are detected and eliminated from further consideration by dot blot analysis using oligonucleotide probes specific for the known genes in the target group. Potentially novel genes are subjected to further sequence analysis to confirm novelty and assayed for biological activity. Compositions of the present invention include novel polynucleotides, and variants and fragments thereof, that comprise novel genes and the polypeptides encoded thereby.
摘要翻译：提供了用于鉴定与目标基因组的已知基因具有同源性区域的新基因的方法和组合物。所述方法包括系统地设计对已知基因的靶组的核苷酸序列内的同源区特异性的寡核苷酸引物，并且从感兴趣的生物体进行连续循环的核酸材料的PCR扩增。 PCR步骤旨在鉴定和扩增包含已知和新基因的核酸。通过使用针对靶组中已知基因特异的寡核苷酸探针的斑点印迹分析，进一步考虑检测并消除包含已知基因的核酸分子。将潜在的新基因进行进一步的序列分析以确认新颖性并测定生物活性。本发明的组合物包括新型多核苷酸及其变体和片段，其包含新基因和由其编码的多肽。

18. 发明申请

US20080020967A1 Unique Novel Bacillus Thuringiensis Gene with Lepidopteran Activity 有权
标题翻译：独特的新型苏云金芽孢杆菌基因与鳞翅目活动
公开(公告)号：US20080020967A1
公开(公告)日：2008-01-24
申请号：US11780503
申请日：2007-07-20
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi
IPC分类号： A01H5/00 , A01N37/18 , C07H21/04 , C12N15/00 , C12N5/04 , C12P21/04
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编码具有杀虫活性的昆虫的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其包括鳞翅目昆虫害虫。本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，DNA构建体和转化的微生物的分离的核酸和包含实施方案的核酸的植物。这些组合物可用于防治害虫，特别是植物害虫的方法。

19. 发明授权

US08878007B2 Bacillus thuringiensis gene with lepidopteran activity 有权
公开(公告)号：US08878007B2
公开(公告)日：2014-11-04
申请号：US13413707
申请日：2012-03-07
申请人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi , Thomas C. Wolfe
发明人： Andre R. Abad , Hua Dong , Sue B. Lo , Xiaomei Shi , Thomas C. Wolfe
IPC分类号： A01H5/00 , C12N15/82 , C12P1/00 , C07K1/00 , C07H21/02 , A01N63/02 , C07K14/325
CPC分类号： C12N15/8279 , A01N63/02 , C07K14/325
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

20. 发明授权

US08692066B2 Bacillus thuringiensis gene with coleopteran activity 失效
标题翻译：具有鞘翅目昆虫活性的苏云金芽孢杆菌基因
公开(公告)号：US08692066B2
公开(公告)日：2014-04-08
申请号：US12963355
申请日：2010-12-08
申请人： Andre R Abad , Hua Dong , Sue B Lo , Xiaomei Shi
发明人： Andre R Abad , Hua Dong , Sue B Lo , Xiaomei Shi
IPC分类号： A01H5/00 , A01H5/10 , C07K14/325 , A01N37/18 , C12N15/11 , C12N15/32 , C12N15/63 , C12N15/82 , C12N1/21 , C12N5/10
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , Y02A40/162
摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
摘要翻译：本发明提供从编码具有杀虫活性的昆虫的苏云金芽孢杆菌菌株获得的核酸及其变体和片段，其包括鞘翅目。本发明的具体实施方案提供编码杀虫蛋白，杀虫组合物，DNA构建体和转化的微生物的分离的核酸和包含实施方案的核酸的植物。这些组合物可用于防治害虫，特别是植物害虫的方法。

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