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11. 发明申请

US20130005041A1 Agent for Improving Gene Transfer Efficiency to Mammalian Cells 有权
标题翻译：用于提高哺乳动物细胞基因转移效率的试剂
公开(公告)号：US20130005041A1
公开(公告)日：2013-01-03
申请号：US13583847
申请日：2011-03-13
申请人： Rinji Akada , Mikiko Nakamura
发明人： Rinji Akada , Mikiko Nakamura
IPC分类号： C12N15/88 , C09K3/00
CPC分类号： C12N15/88
摘要： Provided is an efficiency improving agent for gene transfer to mammalian cells, a method for improving efficiency of gene transfer to mammalian cells, and a method for transforming mammalian cells. The method is characterized in that tRNA is used in combination with a lipofection reagent. Preferably, the agent may be used so that the tRNA concentration in a lipofection solution falls within the range of 3 to 50 μg/mL, and the concentration in a culture is approximately 1/10. More preferably, tRNA and PEG may be used in combination with a lipofection reagent. According to the present invention, gene transfer to mammalian cells with high efficiency can be achieved.
摘要翻译：提供了用于向哺乳动物细胞的基因转移的效率提高剂，提高哺乳动物细胞的基因转移效率的方法和转化哺乳动物细胞的方法。该方法的特征在于tRNA与脂转染试剂组合使用。优选地，可以使用试剂，使得脂转染溶液中的tRNA浓度在3至50μg/ mL的范围内，培养物中的浓度约为1/10。更优选地，tRNA和PEG可以与脂转染试剂组合使用。根据本发明，可以实现以高效率转运到哺乳动物细胞的基因。

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