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    • 11. 发明授权
    • Method for enriching and isolating bacteria which aerobically degrades PCNB
    • 丰富和分离有氧降解PCNB的细菌的方法
    • US06498028B1
    • 2002-12-24
    • US09462877
    • 2000-03-24
    • Kazuhiro TakagiYuuichi Yoshioka
    • Kazuhiro TakagiYuuichi Yoshioka
    • C12N120
    • C12N1/20B09C1/10C12R1/01
    • It is an object of the invention to improve a conventional soil percolation technique to thereby provide a method of enriching and isolating decomposing bacteria decomposing an organochlorine agricultural chemical PCNB which is difficult to decompose, in a short time period, and to provide decomposing bacteria for efficiently processing PCNB. To practice the method, an enrichment soil layer 2 is formed by mixing a soil containing an organochlorine agricultural chemical PCNB with a fragmented porous material having an infinite number of micropores and at the same time a greater adsorptivity for adsorbing PCNB than the soil, and an inorganic salt medium 3 containing a carbon and nitrogen source, the carbon and nitrogen source being formed by only PCNB, is circulated through the enrichment soil layer 2, thereby enriching the aerobic bacteria Burkholderia cepacia in the fragmented porous material.
    • 本发明的目的是改进常规的土壤渗滤技术,从而提供一种在短时间内分解分解有机氯农药PCNB的分解细菌的分解方法,并且能够有效地提供分解细菌 处理PCNB。 为了实施该方法,通过将含有机氯农药PCNB的土壤与具有无数个微孔的碎裂多孔材料混合并同时具有比土壤吸附PCNB更大的吸附性,形成富集土层2, 含有碳源和氮源的无机盐介质3,仅由PCNB形成的碳源和氮源通过富集土层2循环,从而富集碎片多孔材料中的好氧细菌伯克霍尔德菌(Burkholderia cepacia)。
    • 15. 发明授权
    • Microorganism capable of microbially decomposing chloro-, methylthio- and methoxy-triazine agrichemicals
    • 微生物能够微量分解氯,甲硫基和甲氧基三嗪农药
    • US08168420B2
    • 2012-05-01
    • US12513573
    • 2007-11-20
    • Kazuhiro TakagiKunihiko FujiiNaoki HaradaAkio Iwasaki
    • Kazuhiro TakagiKunihiko FujiiNaoki HaradaAkio Iwasaki
    • C12N1/20C02F3/34A62D3/02
    • B09C1/10A62D3/02A62D2101/26C02F3/34C02F2101/306C12R1/01Y10S435/822
    • Disclosed are: a novel microorganism which can decompose a methylthiotriazine compound (particularly, simetryn, dimethametryn, prometryn), a chlorotriazine compound (particularly, simazine, atrazine, propazine) and a methoxytriazine compound (particularly, simeton, atraton, prometon) which have been frequently used as agrichemicals or the like; and a method for decomposing a methylthiotriazine compound, a chlorotriazine compound and/or a methoxytriazine compound by using the microorganism. Specifically disclosed are: a novel bacterium Nocardioides sp. strain MTD22 which is capable of decomposing a methylthiotriazine compound, a chlorotriazine compound and a methoxytriazine compound; and a method for decomposing a methylthiotriazine compound, a chlorotriazine compound and/or a methoxytriazine compound, particularly simetryn, dimethametryn, prometryn, simazine, atrazine, propazine, simeton, atraton and/or prometon, by using the microorganism.
    • 公开了一种可以分解甲基硫代三嗪化合物(特别是西米特,二甲基丙烯酸甲酯,prometryn),氯代三嗪化合物(特别是西马拉嗪,莠去津,丙嗪)和甲氧基三嗪化合物(特别是西蒙特,阿特拉津,普罗特酮)的新型微生物, 经常用作农药等; 以及通过使用微生物分解甲基硫代三嗪化合物,氯代三嗪化合物和/或甲氧基三嗪化合物的方法。 具体公开的是:新型细菌诺卡氏菌属 能够分解甲基硫代三嗪化合物,氯代三嗪化合物和甲氧基三嗪化合物的菌株MTD22; 以及通过使用该微生物分解甲基硫代三嗪化合物,氯代三嗪化合物和/或甲氧基三嗪化合物,特别是西蒙特,二甲基丙烯酸,衍生物,西马拉平,莠去津,丙嗪,西蒙,阿特拉津和/或前导子的方法。
    • 16. 发明授权
    • Microorganism capable of degrading diphenylarsinic acid
    • 能够降解二苯亚胂酸的微生物
    • US08148134B2
    • 2012-04-03
    • US12439662
    • 2007-08-24
    • Kazuhiro TakagiNaoki Harada
    • Kazuhiro TakagiNaoki Harada
    • C12N1/12C12F3/34
    • C12R1/01A62D3/02A62D2101/02A62D2101/20A62D2101/24B09C1/10C02F3/34C07F9/76C12N1/20
    • Disclosed are: a microorganism capable of degrading diphenylarsinic acid; a method for degrading diphenylarsinic acid by using the microorganism; a method for clean-up of a contaminated soil by using the microorganism; an agent for degrading diphenylarsinic acid, which comprises the microorganism; and a cleaning agent for a contaminated soil or groundwater, which comprises the microorganism. Specifically disclosed are: a microorganism belonging to the genus Sinorhizobium and capable of degrading diphenylarsinic acid; a microorganism belonging to the genus Ensifer and capable of degrading diphenylarsinic acid; a method for clean-up of a contaminated soil by using the microorganism; an agent for degrading diphenylarsinic acid, which comprises the microorganism; and a cleaning agent for a contaminated soil or groundwater, which comprises the microorganism.
    • 公开了能够降解二苯亚胂酸的微生物; 通过使用微生物降解二苯亚胂酸的方法; 通过使用微生物清理污染土壤的方法; 包含微生物的降解二苯亚胂酸的试剂; 以及包含微生物的污染土壤或地下水的清洁剂。 具体公开的是:属于中华根瘤菌属的微生物,能够降解二苯亚胂酸; 属于Ensifer属的微生物,能够降解二苯亚胂酸; 通过使用微生物清理污染土壤的方法; 包含微生物的降解二苯亚胂酸的试剂; 以及包含微生物的污染土壤或地下水的清洁剂。
    • 18. 发明申请
    • NOVEL MICROORGANISM CAPABLE OF DEGRADING DIPHENYLARSINIC ACID
    • 可降解二苯甲酸的新型微生物
    • US20100015688A1
    • 2010-01-21
    • US12439662
    • 2007-08-24
    • Kazuhiro TakagiNaoki Harada
    • Kazuhiro TakagiNaoki Harada
    • C12N1/20C02F3/34
    • C12R1/01A62D3/02A62D2101/02A62D2101/20A62D2101/24B09C1/10C02F3/34C07F9/76C12N1/20
    • Disclosed are: a microorganism capable of degrading diphenylarsinic acid; a method for degrading diphenylarsinic acid by using the microorganism; a method for clean-up of a contaminated soil by using the microorganism; an agent for degrading diphenylarsinic acid, which comprises the microorganism; and a cleaning agent for a contaminated soil or groundwater, which comprises the microorganism. Specifically disclosed are: a microorganism belonging to the genus Sinorhizobium and capable of degrading diphenylarsinic acid; a microorganism belonging to the genus Ensifer and capable of degrading diphenylarsinic acid; a method for clean-up of a contaminated soil by using the microorganism; an agent for degrading diphenylarsinic acid, which comprises the microorganism; and a cleaning agent for a contaminated soil or groundwater, which comprises the microorganism.
    • 公开了能够降解二苯亚胂酸的微生物; 通过使用微生物降解二苯亚胂酸的方法; 通过使用微生物清理污染土壤的方法; 包含微生物的降解二苯亚胂酸的试剂; 以及包含微生物的污染土壤或地下水的清洁剂。 具体公开的是:属于中华根瘤菌属的微生物,能够降解二苯亚胂酸; 属于Ensifer属的微生物,能够降解二苯亚胂酸; 通过使用微生物清理污染土壤的方法; 包含微生物的降解二苯亚胂酸的试剂; 以及包含微生物的污染土壤或地下水的清洁剂。
    • 20. 发明授权
    • Nocardioides FERM BP-10405 capable of degrading organochlorine pesticide PCNB
    • Nocardioides FERM BP-10405能够降解有机氯农药PCNB
    • US07629159B2
    • 2009-12-08
    • US11335686
    • 2006-01-20
    • Kazuhiro TakagiNaoki HaradaYuuichi Yoshioka
    • Kazuhiro TakagiNaoki HaradaYuuichi Yoshioka
    • C12N1/20C12N11/14C12N11/02C12N11/04A62D3/02
    • C12P39/00B09C1/002B09C1/10C12N11/02C12N11/12C12R1/01C12R1/06Y10S435/822Y10S435/83
    • The present invention provides a stable complex microbial system, which simultaneously decomposes a plurality of organic contaminants even under a polluted environment with these contaminants and permits more effective decomposition of persistent organic contaminants such as PCNB and simazine. A support for holding a complexed enrichment of degrading bacteria, which contains a porous material provided as a support on which degrading bacteria A capable of degrading at least one organic contaminant and degrading bacteria B capable of degrading another organic contaminant are enriched, is produced. The degrading bacteria A may be a PCNB-degrading bacteria, particularly degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of Nocardioides sp. PD653 and the degrading bacteria B may be degrading bacteria containing degrading bacteria having part or all of the bacteriological characteristics of β-Proteobacteria CDB21.
    • 本发明提供了一种稳定的复合微生物系统,即使在这些污染物的污染环境下也能同时分解多种有机污染物,并允许持久性有机污染物如PCNB和西马嗪的更有效分解。 产生含有多孔材料的支持物,其含有提供作为支持物的多孔材料,所述多孔材料能够使能够降解至少一种有机污染物的降解细菌A和能够降解另一种有机污染物的降解菌体B被富集。 降解细菌A可以是PCNB降解细菌,特别是含有降解菌的降解细菌,其具有诺卡氏菌属(Nocardioides sp。)的部分或全部细菌学特征。 PD653和降解细菌B可能是含有降解细菌的降解细菌,具有部分或全部β-变形杆菌CDB21的细菌学特征。