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11. 发明申请

US20090105465A1 Protein Purification Using HCIC and Ion Exchange Chromatography 有权
标题翻译：使用HCIC和离子交换色谱法进行蛋白质纯化
公开(公告)号：US20090105465A1
公开(公告)日：2009-04-23
申请号：US11918155
申请日：2006-03-21
申请人： Alahari Arunakumari , Gisela Maria Marques Ferreira
发明人： Alahari Arunakumari , Gisela Maria Marques Ferreira
IPC分类号： C07K1/18
CPC分类号： C07K16/00 , A61K39/39525 , C07K1/18 , C07K1/20 , C07K1/36 , C07K16/28 , C07K16/2878
摘要： The present invention provides methods for purifying proteins. In particular, the methods employ a two-step non-affinity chromatography process without the use of an in-process tangential flow filtration step.
摘要翻译：本发明提供蛋白质的纯化方法。特别地，该方法采用两步非亲和层析方法，而不使用在线切向流过滤步骤。

12. 发明授权

US08357514B2 Methods of gene amplification and expression 有权
标题翻译：基因扩增和表达方法
公开(公告)号：US08357514B2
公开(公告)日：2013-01-22
申请号：US12593132
申请日：2008-03-27
申请人： Alahari Arunakumari , Xiao-Ping Dai , Haile Ghebramariam
发明人： Alahari Arunakumari , Xiao-Ping Dai , Haile Ghebramariam
IPC分类号： C12P21/06 , C12N15/63
CPC分类号： C12N5/0682 , C12N9/003 , C12N15/85 , C12N2840/10 , C12P21/02
摘要： Disclosed are methods relating to amplification and expression of a nucleic acid sequence encoding a polypeptide of interest in recombinant cells, and cell lines and polypeptides produced from such methods. The methods disclosed herein permit the amplification of cell lines that express a polypeptide of interest in a relatively short period of time through the use of a bioreactor.
摘要翻译：公开了与重组细胞中编码目的多肽的核酸序列的扩增和表达有关的方法，以及由这些方法产生的细胞系和多肽。本文公开的方法允许通过使用生物反应器在相对短的时间段内扩增表达目的多肽的细胞系。

13. 发明申请

US20120178910A1 CATION EXCHANGE CHROMATOGRAPHY (METHODS) 审中-公开
标题翻译：交换色谱法（方法）
公开(公告)号：US20120178910A1
公开(公告)日：2012-07-12
申请号：US13497846
申请日：2010-09-22
申请人： Alahari Arunakumari , Jue Wang
发明人： Alahari Arunakumari , Jue Wang
IPC分类号： C07K16/00 , C07K14/00 , C07K1/14
CPC分类号： C07K1/18
摘要： The present invention provides improved methods of protein purification using CEX chromatography. Such methods generally comprise the steps of: contacting a protein of interest (e.g., an antibody) with a cation exchange resin at a first pH, that is less than the pI of the most acidic isoform of the protein of interest, such that the protein of interest binds to the resin; washing the cation exchange resin at a second pH that is greater than the first pH, but less than the pI of the most acidic isoform of the protein of interest; and eluting the protein of interest from the resin at a third pH that is about equal to or less than the first pH. The methods of the invention are particularly useful for the commercial purification of recombinant therapeutic proteins (e.g., antibodies).
摘要翻译：本发明提供使用CEX色谱法的改进的蛋白质纯化方法。这样的方法通常包括以下步骤：使感兴趣的蛋白质（例如，抗体）与阳离子交换树脂在第一pH下接触，其小于感兴趣的蛋白质的最酸性同种型的pI，使得蛋白质的兴趣与树脂结合; 在大于所述第一pH但是小于所述感兴趣蛋白质的最酸性同种型的pI的第二pH下洗涤所述阳离子交换树脂; 并在约等于或小于第一pH的第三pH下从树脂中洗脱目标蛋白质。本发明的方法对于重组治疗性蛋白质（例如抗体）的商业纯化特别有用。

14. 发明授权

US08129508B2 Protein purification using HCIC and ion exchange chromatography 有权
标题翻译：使用HCIC和离子交换色谱法进行蛋白质纯化
公开(公告)号：US08129508B2
公开(公告)日：2012-03-06
申请号：US11918155
申请日：2006-03-21
申请人： Alahari Arunakumari , Gisela Maria Marques Ferreira
发明人： Alahari Arunakumari , Gisela Maria Marques Ferreira
IPC分类号： C07K16/00 , C07K1/18 , C07K1/20
CPC分类号： C07K16/00 , A61K39/39525 , C07K1/18 , C07K1/20 , C07K1/36 , C07K16/28 , C07K16/2878
摘要： The present invention provides methods for purifying proteins. In particular, the methods employ a two-step non-affinity chromatography process without the use of an in-process tangential flow filtration step.
摘要翻译：本发明提供蛋白质的纯化方法。特别地，该方法采用两步非亲和层析方法，而不使用在线切向流过滤步骤。

15. 发明申请

US20100261886A1 PROTEIN PURIFICATION BY CITRATE PRECIPITATION 有权
标题翻译：蛋白质纯化由蛋白质降解
公开(公告)号：US20100261886A1
公开(公告)日：2010-10-14
申请号：US12759016
申请日：2010-04-13
申请人： Alahari Arunakumari , Gisela M. Ferreira
发明人： Alahari Arunakumari , Gisela M. Ferreira
IPC分类号： C07K1/36 , C07K1/30 , C07K16/00
CPC分类号： C07K1/30 , C07K1/36
摘要： The invention provides methods for isolating proteins in purified form from mixtures by precipitation with citrate. The methods are advantageous in that they effectively separate a protein from lower molecular weight contaminants, including fragments or portions of the protein. Such methods are particularly useful for purifying antibodies from mixtures containing antibody proteolytic fragments and unpaired chains.
摘要翻译：本发明提供了通过用柠檬酸盐沉淀从混合物中分离纯化形式的蛋白质的方法。该方法的优点在于它们有效地将蛋白质与低分子量污染物（包括蛋白质的片段或部分）分离。这样的方法特别可用于从含有抗体蛋白水解片段和未配对链的混合物中纯化抗体。

16. 发明申请

US20100178673A1 Methods of Gene Amplification and Expression 有权
标题翻译：基因扩增和表达方法
公开(公告)号：US20100178673A1
公开(公告)日：2010-07-15
申请号：US12593132
申请日：2008-03-27
申请人： Alahari Arunakumari , Xiao-Ping Dai
发明人： Alahari Arunakumari , Xiao-Ping Dai
IPC分类号： C12P21/06 , C12N15/63
CPC分类号： C12N5/0682 , C12N9/003 , C12N15/85 , C12N2840/10 , C12P21/02
摘要： Disclosed are methods relating to amplification and expression of a nucleic acid sequence encoding a polypeptide of interest in recombinant cells, and cell lines and polypeptides produced from such methods. The methods disclosed herein permit the amplification of cell lines that express a polypeptide of interest in a relatively short period of time through the use of a bioreactor.
摘要翻译：公开了与重组细胞中编码目的多肽的核酸序列的扩增和表达有关的方法，以及由这些方法产生的细胞系和多肽。本文公开的方法允许通过使用生物反应器在相对短的时间段内扩增表达目的多肽的细胞系。

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