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11. 发明授权

US06654165B2 Optical arrangement 有权
标题翻译：光学布置
公开(公告)号：US06654165B2
公开(公告)日：2003-11-25
申请号：US10342750
申请日：2003-01-15
申请人： Johann Engelhardt , Joachim Bradl , Heinrich Ulrich
发明人： Johann Engelhardt , Joachim Bradl , Heinrich Ulrich
IPC分类号： G02B2106
CPC分类号： G02B21/0076 , G02B21/0024
摘要： An optical arrangement in the beam path of a light source suitable for fluorescence excitation, preferably in the beam path of a confocal laser scanning microscope, with at least one spectrally selective element (4) to inject the excitation light (3) of at least one light source (2) in the microscope and to extract the excitation light (3) scattered and reflected on the object (10) or the excitation wavelength from the light (13) coming from the object (10) through the detection beam path (12) is characterized for variable configurations with the simplest construction in that excitation light (3, 9) of different wavelengths can be extracted by the spectrally selective element (4). Alternatively, an optical arrangement like this is characterized in that the spectrally selective element (4) can be adjusted to the excitation wavelength to be extracted.
摘要翻译：适合于荧光激发的光源的光路中的光学装置，优选地在共聚焦激光扫描显微镜的光束路径中，具有至少一个光谱选择性元件（4）以将至少一个激发光（3）注入光源（2），并且通过检测光束路径（12）提取从物体（10）的光（13）散射和反射的激发光（3）或来自物体（10）的激发波长）的特征在于具有最简单结构的可变配置，其中不同波长的激发光（3,9）可以由光谱选择元件（4）提取。或者，这样的光学布置的特征在于，可以将光谱选择元件（4）调节到要提取的激发波长。

12. 发明授权

US06510001B1 Optical arrangement with a spectrally selective element 有权
标题翻译：具有光谱选择性元件的光学布置
公开(公告)号：US06510001B1
公开(公告)日：2003-01-21
申请号：US09622489
申请日：2000-08-17
申请人： Johann Engelhardt , Joachim Bradl , Heinrich Ulrich
发明人： Johann Engelhardt , Joachim Bradl , Heinrich Ulrich
IPC分类号： G02B2106
CPC分类号： G02B21/0076 , G02B21/0024
摘要： An optical arrangement in the beam path of a light source suitable for fluorescence excitation, preferably in the beam path of a confocal laser scanning microscope, with at least one spectrally selective element (4) to inject the excitation light (3) of at least one light source (2) in the microscope and to extract the excitation light (3) scattered and reflected on the object or the excitation wavelength from the light (13) coming from the object (10) through the detection beam path(12) is characterized for variable configuration with the simplest construction in that excitation light (3, 9) of different wavelengths can be extracted by the spectrally selective element (4). Alternatively, an optical arrangement like this is characterized in that the spectrally selective element (4) can be adjusted to the excitation wavelength to be extracted.
摘要翻译：适合于荧光激发的光源的光路中的光学装置，优选地在共聚焦激光扫描显微镜的光束路径中，具有至少一个光谱选择性元件（4）以将至少一个激发光（3）注入显微镜中的光源（2）和从物体（10）穿过检测光路（12）散射并反射在物体上或激发波长上的激发光（3）被提取出来对于具有最简单结构的可变配置，其中不同波长的激发光（3,9）可以由光谱选择元件（4）提取。或者，这样的光学布置的特征在于，可以将光谱选择元件（4）调节到要提取的激发波长。

13. 发明授权

US06678089B1 Microscope 失效
标题翻译：显微镜
公开(公告)号：US06678089B1
公开(公告)日：2004-01-13
申请号：US09549026
申请日：2000-04-13
申请人： Johann Engelhardt , Joachim Bradl
发明人： Johann Engelhardt , Joachim Bradl
IPC分类号： G02B2100
CPC分类号： G02B21/0024
摘要： A microscope (1), preferably a confocal laser scanning microscope, having at least one light source, a detector, and two objectives (2), one of the objectives (2) being arranged on each of the two sides of the specimen plane (3) and the objectives (2) being directed toward one another and having a common focus; and at least one beam splitter (5) for distributing the illuminating light (6) to the objectives (2), and a beam recombiner (5) for combining the detected light (7) coming from the objectives (2), being provided in the illumination/detection beam path (4), is characterized, for selectable, subsequent implementation of ultrahigh-resolution microscope techniques, in that the objectives (2) and the beam splitter/beam recombiner (5) are grouped into a modular assembly (8); and the assembly (8) has an interface (9) for connection to the illumination/detection beam path (4) of the microscope (1).
摘要翻译：具有至少一个光源，检测器和两个物镜（2）的显微镜（1），优选共聚焦激光扫描显微镜，物镜（2）中的一个被布置在样品平面的两侧 3）和目标（2）相互指向并共同关注; 以及用于将照明光（6）分配到物镜（2）的至少一个分束器（5）和用于组合来自物镜（2）的检测光（7）的光束重组器（5），其设置在其特征在于，为了可选择地实现超高分辨率显微镜技术，其目的（2）和分束器/光束重组器（5）被分组成模块化组件（8））; 并且组件（8）具有用于连接到显微镜（1）的照明/检测光束路径（4）的接口（9）。

14. 发明授权

US07031055B2 Microscope 失效
标题翻译：显微镜
公开(公告)号：US07031055B2
公开(公告)日：2006-04-18
申请号：US10717524
申请日：2003-11-21
申请人： Johann Engelhardt , Joachim Bradl
发明人： Johann Engelhardt , Joachim Bradl
IPC分类号： G02B21/06
CPC分类号： G02B21/0024
摘要： A microscope (1), preferably a confocal laser scanning microscope, having at least one light source, a detector, and two objectives (2), one of the objectives (2) being arranged on each of the two sides of the specimen plane (3) and the objectives (2) being directed toward one another and having a common focus; and at least one beam splitter (5) for distributing the illuminating light (6) to the objectives (2), and a beam recombiner (5) for combining the detected light (7) coming from the objectives (2), being provided in the illumination/detection beam path (4), is characterized, for selectable, subsequent implementation of ultrahigh-resolution microscope techniques, in that the objectives (2) and the beam splitter/beam recombiner (5) are grouped into a modular assembly (8); and the assembly (8) has an interface (9) for connection to the illumination/detection beam path (4) of the microscope (1).
摘要翻译：具有至少一个光源，检测器和两个物镜（2）的显微镜（1），优选共聚焦激光扫描显微镜，物镜（2）中的一个被布置在样品平面的两侧 3）和目标（2）相互指向并共同关注; 以及用于将照明光（6）分配到物镜（2）的至少一个分束器（5）和用于组合来自物镜（2）的检测光（7）的光束重组器（5），其设置在其特征在于，为了可选择地实现超高分辨率显微镜技术，其目的（2）和分束器/光束重组器（5）被分组成模块化组件（8））; 并且组件（8）具有用于连接到显微镜（1）的照明/检测光束路径（4）的接口（9）。

15. 发明授权

US06614525B1 Laser scanning microscope with single wavelength excitation 有权
标题翻译：激光扫描显微镜，单波长激发
公开(公告)号：US06614525B1
公开(公告)日：2003-09-02
申请号：US09718948
申请日：2000-11-20
申请人： Johann Engelhardt , Joachim Bradl
发明人： Johann Engelhardt , Joachim Bradl
IPC分类号： G01J330
CPC分类号： G02B21/0032 , G02B21/0064
摘要： A laser scanning microscope, preferably a confocal laser scanning microscope, having a laser light source for illuminating a specimen and a detector for detecting the light returning from the specimen, the specimen or parts thereof. The specimen is marked with markers that can be excited to emit. For the specific detection of preferably biological specimen structures, with high localization accuracy for the specimen structures, the laser light source emits exciting light substantially at one wavelength. Different markers emit light of different wavelengths, when irradiated with exciting light of substantially the same wavelength. The detector is embodied as a multi-band detector for the simultaneous detection of light at several wavelengths. A corresponding method for the detection of preferably biological specimens or specimen structures by laser scanning microscopy is described.
摘要翻译：激光扫描显微镜，优选共焦激光扫描显微镜，具有用于照射样本的激光光源和用于检测从样本返回的光的检测器，其样本或其部分。标本上标有可被激发发射的标记。对于优选生物样本结构的具体检测，对于样本结构具有高定位精度，激光光源基本上发射一个波长的激发光。当用基本上相同波长的激发光照射时，不同的标记发射不同波长的光。检测器被实现为用于同时检测几个波长的光的多波段检测器。描述了通过激光扫描显微镜检测优选生物样品或样本结构的相应方法。

16. 发明授权

US06909540B2 Microscope objective, microscope, and method for imaging a specimen 有权
标题翻译：显微镜目标，显微镜和成像成像方法
公开(公告)号：US06909540B2
公开(公告)日：2005-06-21
申请号：US10308608
申请日：2002-12-02
申请人： Johann Engelhardt , Rafael Storz
发明人： Johann Engelhardt , Rafael Storz
IPC分类号： G02B7/04 , G02B21/00 , G02B21/02 , G02B21/24
CPC分类号： G02B21/02 , G02B21/241
摘要： A microscope objective that comprises an objective housing and contains several lens elements, at least one lens element being arranged displaceably in motor-driven fashion within the objective housing, is disclosed. A microscope and a method for imaging a specimen are additionally disclosed.
摘要翻译：公开了一种显微镜物镜，其包括物镜壳体并且包含若干透镜元件，至少一个透镜元件以电机驱动的方式在物镜壳体内可移位地布置。另外公开了用于成像试样的显微镜和方法。

17. 发明授权

US07016101B2 Scanning microscope and optical element 有权
公开(公告)号：US07016101B2
公开(公告)日：2006-03-21
申请号：US11012523
申请日：2004-12-15
申请人： Holger Birk , Johann Engelhardt
发明人： Holger Birk , Johann Engelhardt
IPC分类号： G02F1/33
CPC分类号： G02B21/0024 , G02B21/0032 , G02B21/0068 , G02B21/0076
摘要： A scanning microscope that defines an illumination beam path and a detection beam path, having an objective that is arranged in both the illumination beam path and the detection beam path, is disclosed. The scanning microscope is characterized by an interchangeable module that is also arranged in the illumination beam path and a [sic] detection beam path and that separates the illumination beam path and detection beam path at a fixed angular relationship to one another and comprises at least a first acoustooptical component. Also disclosed is an optical element having at least three ports.

18. 发明申请

US20050099673A1 Scanning microscope and optical element 有权
标题翻译：扫描显微镜和光学元件
公开(公告)号：US20050099673A1
公开(公告)日：2005-05-12
申请号：US11012523
申请日：2004-12-15
申请人： Holger Birk , Johann Engelhardt
发明人： Holger Birk , Johann Engelhardt
IPC分类号： G02B21/18 , G02B21/00 , G02B21/06 , G02F1/33
CPC分类号： G02B21/0024 , G02B21/0032 , G02B21/0068 , G02B21/0076
摘要： A scanning microscope that defines an illumination beam path and a detection beam path, having an objective that is arranged in both the illumination beam path and the detection beam path, is disclosed. The scanning microscope is characterized by an interchangeable module that is also arranged in the illumination beam path and a [sic] detection beam path and that separates the illumination beam path and detection beam path at a fixed angular relationship to one another and comprises at least a first acoustooptical component. Also disclosed is an optical element having at least three ports.
摘要翻译：公开了一种限定照明光束路径和检测光束路径的扫描显微镜，其具有布置在照明光束路径和检测光束路径两者中的物镜。扫描显微镜的特征在于可互换模块，其也布置在照明光束路径和检测光束路径中，并且将照射光束路径和检测光束路径彼此固定的角度关系分开，并且包括至少一个第一声光组件。还公开了具有至少三个端口的光学元件。

19. 发明授权

US06850358B2 Scanning microscope and optical element 有权
标题翻译：扫描显微镜和光学元件
公开(公告)号：US06850358B2
公开(公告)日：2005-02-01
申请号：US10207428
申请日：2002-07-29
申请人： Holger Birk , Johann Engelhardt
发明人： Holger Birk , Johann Engelhardt
IPC分类号： G02B21/18 , G02B21/00 , G02B21/06 , G02F1/33
CPC分类号： G02B21/0024 , G02B21/0032 , G02B21/0068 , G02B21/0076
摘要： A scanning microscope that defines an illumination beam path and a detection beam path, having an objective that is arranged in both the illumination beam path and the detection beam path, is disclosed. The scanning microscope is characterized by an interchangeable module that is also arranged in the illumination beam path and a detection beam path and that separates the illumination beam path and detection beam path at a fixed angular relationship to one another and comprises at least a first acoustooptical component. Also disclosed is an optical element having at least three ports.
摘要翻译：公开了一种限定照明光束路径和检测光束路径的扫描显微镜，其具有布置在照明光束路径和检测光束路径两者中的物镜。扫描显微镜的特征在于可互换模块，其也布置在照明光束路径和检测光束路径中，并且将照明光束路径和检测光束路径以固定的角度关系彼此分离，并且包括至少第一声光分量。还公开了具有至少三个端口的光学元件。

20. 发明授权

US06567212B1 Vibration damping device for microscopes and microscope with a vibration damping device 有权
标题翻译：具有减振装置的显微镜和显微镜振动阻尼装置
公开(公告)号：US06567212B1
公开(公告)日：2003-05-20
申请号：US09639592
申请日：2000-08-16
申请人： Johann Engelhardt , Rafael Storz
发明人： Johann Engelhardt , Rafael Storz
IPC分类号： G02B2100
CPC分类号： G02B21/24
摘要： A Vibration damping device for a microscope comprises a housing. In the housing a foam rubber defining a cavity is formed which encloses a weight. The foam rubber has a plurality of interconnected pores. The housing is of the vibration damping device has in one embodiment a rectangular shape.
摘要翻译：用于显微镜的振动阻尼装置包括壳体。在壳体中形成限定空腔的泡沫橡胶，其包围重物。泡沫橡胶具有多个互连孔。在一个实施例中，壳体是减振装置的矩形形状。

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