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11. 发明申请

US20120288942A1 USE OF MEGANUCLEASES FOR INDUCING HOMOLOGOUS RECOMBINATION EX VIVO AND IN TOTO IN VERTEBRATE SOMATIC TISSUES AND APPLICATION THEREOF 审中-公开
公开(公告)号：US20120288942A1
公开(公告)日：2012-11-15
申请号：US13556208
申请日：2012-07-24
申请人： Sylvain Arnould , Patrick Chames , Philippe Duchateau , Jean-Charles Epinat , Emmanuel Lacroix , Frédéric Paques
发明人： Sylvain Arnould , Patrick Chames , Philippe Duchateau , Jean-Charles Epinat , Emmanuel Lacroix , Frédéric Paques
IPC分类号： C12N15/01
CPC分类号： C12N15/907 , A01K67/0275 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/105 , A01K2267/03 , A01K2267/0337 , A61K38/00 , A61K38/1709 , A61K38/465 , A61K48/00 , A61K48/0058 , C07K14/435 , C07K2319/81 , C12N7/00 , C12N9/22 , C12N15/1058 , C12N15/8509 , C12N15/86 , C12N15/90 , C12N2730/10143 , C12N2799/022 , C12N2800/80 , C12N2830/002 , C12N2830/55 , C12N2840/20 , C12N2840/44 , C12Y301/00 , C12Y301/21004
摘要： Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and to its application for genome engineering and gene therapy.

12. 发明申请

US20100021448A1 I-CREI MEGANUCLEASE VARIANTS WITH MODIFIED SPECIFICITY, METHOD OF PREPARATION AND USES THEREOF 无效
标题翻译：具有改性特异性的I-CREI MEGANUCLEASE变体，其制备方法及其用途
公开(公告)号：US20100021448A1
公开(公告)日：2010-01-28
申请号：US11908798
申请日：2006-03-15
申请人： Philippe Duchateau , Frederic Paques
发明人： Philippe Duchateau , Frederic Paques
IPC分类号： A61K38/46 , C12N9/22 , C12N15/11 , C12N15/00 , C12N5/10 , C12N5/04 , A01H5/00 , A01K67/027 , C12P19/34 , A61L2/18 , A61K31/711
CPC分类号： C12N9/22 , A61K38/00 , A61K48/00
摘要： Method of preparing 1-CreI meganuclease variants having a modified cleavage specificity, variants obtainable by said method and their applications either for cleaving new DNA target or for genetic engineering and genome engineering for non-therapeutic purposes. Nucleic acids encoding said variants, expression cassettes comprising said nucleic acids, vectors comprising said expression cassettes, cells or organisms, plants or animals except humans, transformed by said vectors.
摘要翻译：制备具有修饰的切割特异性的1-CreI大范围核酸酶变体的方法，通过所述方法获得的变体及其用于切割新的DNA靶或用于非治疗目的的基因工程和基因工程的应用。编码所述变体的核酸，包含所述核酸的表达盒，包含所述表达盒的载体，细胞或生物，除人以外的植物或动物，由所述载体转化。

13. 发明授权

US10815500B2 Transcription activator-like effector (TALE) fusion protein 有权
公开(公告)号：US10815500B2
公开(公告)日：2020-10-27
申请号：US14405283
申请日：2013-06-05
申请人： Alexandre Juillerat , Philippe Duchateau , Cellectis
发明人： Alexandre Juillerat , Philippe Duchateau
IPC分类号： C12N15/90 , C12N9/22 , C12N15/10
摘要： The present invention relates to Transcription Activator-Like Effector (TALE) derived proteins that allow efficient targeting and/or processing of double stranded nucleic acid sequences. The proteins of the invention are typically chimeric protein monomers composed of a core scaffold comprising Repeat Variable Dipeptide regions (RVDs) having binding specificity to a DNA target sequence, to which is fused a catalytic domain to its N-terminus. This later catalytic domain, which can be a monomer of a nuclease, is placed at this position to possibly interact with another catalytic domain fused to another TAL monomer, such that, when the monomers are binding to their respective target DNA sequences, both catalytic domains form a catalytic entity likely to process DNA in the proximity of these target sequences. This new TAL architecture makes it possible to target only one DNA strand, which is not the case, for instance, with classical TALEN architectures. The present invention also relates to vectors encoding such proteins and compositions or kits in which Transcription Activator-Like Effector (TALE) proteins of the present invention are used.

14. 发明申请

US20120159659A1 CUSTOM-MADE MEGANUCLEASE AND USE THEREOF 审中-公开
标题翻译：自定义MEGANUCLEASE及其使用
公开(公告)号：US20120159659A1
公开(公告)日：2012-06-21
申请号：US12892635
申请日：2010-09-28
申请人： Sylvain Arnould , Sylvia Bruneau , Jean-Pierre Cabaniols , Patrick Chames , André Choulika , Philippe Duchateau , Jean-Pierre Epinat , Agnès Gouble , Emmanuel Lacroix , Frédéric Paques , Christophe Perez-Michaut , Julianne Smith , David Sourdive
发明人： Sylvain Arnould , Sylvia Bruneau , Jean-Pierre Cabaniols , Patrick Chames , André Choulika , Philippe Duchateau , Jean-Pierre Epinat , Agnès Gouble , Emmanuel Lacroix , Frédéric Paques , Christophe Perez-Michaut , Julianne Smith , David Sourdive
IPC分类号： A01K67/027 , C12N15/55 , C12N1/19 , C12N1/21 , A01H5/00 , C12N9/22 , C12N15/63
CPC分类号： C12N15/907 , A01K67/0275 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/105 , A01K2267/03 , A01K2267/0337 , A61K38/00 , A61K38/1709 , A61K38/465 , A61K48/00 , A61K48/0058 , C07K14/435 , C07K2319/81 , C12N7/00 , C12N9/22 , C12N15/1058 , C12N15/8509 , C12N15/86 , C12N15/90 , C12N2730/10143 , C12N2799/022 , C12N2800/80 , C12N2830/002 , C12N2830/55 , C12N2840/20 , C12N2840/44 , C12Y301/00 , C12Y301/21004
摘要： New rare-cutting endonucleases, also called custom-made meganucleases, which recognize and cleave a specific nucleotide sequence, derived polynucleotide sequences, recombinant vector cell, animal, or plant comprising said polynucleotide sequences, process for producing said rare-cutting endonucleases and any use thereof, more particularly, for genetic engineering, antiviral therapy and gene therapy.
摘要翻译：新的稀释内切核酸酶，也称为定制的大范围核酸酶，其识别和切割特定的核苷酸序列，衍生的多核苷酸序列，包含所述多核苷酸序列的重组载体细胞，动物或植物，用于产生所述稀少内切核酸酶的方法和任何用途更具体地，涉及基因工程，抗病毒治疗和基因治疗。

15. 发明申请

US20110287513A1 USE OF MEGANUCLEASES FOR INDUCING HOMOLOGOUS RECOMBINATION EX VIVO AND IN TOTO IN VERTEBRATE SOMATIC TISSUES AND APPLICATION THEREOF 有权
标题翻译：用于诱导异体重组和血栓形成的皮质组织中的巨细胞病毒的应用及其应用
公开(公告)号：US20110287513A1
公开(公告)日：2011-11-24
申请号：US13152961
申请日：2011-06-03
申请人： Sylvain Arnould , Sylvia Bruneau , Jean-Pierre Cabaniols , Patrick Chames , Andre Choulika , Philippe Duchateau , Jean-Charles Epinat , Agnes Gouble , Emmanuel Lacroix , Frederic Paques , Christophe Perez-Michaut , Julianne Smith , David Sourdive
发明人： Sylvain Arnould , Sylvia Bruneau , Jean-Pierre Cabaniols , Patrick Chames , Andre Choulika , Philippe Duchateau , Jean-Charles Epinat , Agnes Gouble , Emmanuel Lacroix , Frederic Paques , Christophe Perez-Michaut , Julianne Smith , David Sourdive
IPC分类号： C12N9/16
CPC分类号： C12N15/907 , A01K67/0275 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/105 , A01K2267/03 , A01K2267/0337 , A61K38/00 , A61K38/1709 , A61K38/465 , A61K48/00 , A61K48/0058 , C07K14/435 , C07K2319/81 , C12N7/00 , C12N9/22 , C12N15/1058 , C12N15/8509 , C12N15/86 , C12N15/90 , C12N2730/10143 , C12N2799/022 , C12N2800/80 , C12N2830/002 , C12N2830/55 , C12N2840/20 , C12N2840/44 , C12Y301/00 , C12Y301/21004
摘要： A single chain homing endonuclease, comprising a first variant of I-CreI having the amino acid sequence of accession number pdb 1g9y and a second variant of I-CreI variant having the amino acid sequence of accession number pdb 1g9y in a single polypeptide.
摘要翻译：单链归巢内切核酸酶，其包含具有登录号pdb 1g9y的氨基酸序列的I-CreI的第一变体和在单个多肽中具有登录号pdb 1g9y的氨基酸序列的I-CreI变体的第二变体。

16. 发明申请

US20110207199A1 NOVEL METHOD TO GENERATE MEGANUCLEASES WITH ALTERED CHARACTERISTICS 审中-公开
标题翻译：用改进的特征生成核糖体的新方法
公开(公告)号：US20110207199A1
公开(公告)日：2011-08-25
申请号：US13057528
申请日：2009-02-09
申请人： Frederic Paques , Sylvestre Grizot , Philippe Duchateau
发明人： Frederic Paques , Sylvestre Grizot , Philippe Duchateau
IPC分类号： C12N9/16 , C40B10/00
CPC分类号： C12P19/34 , C12N9/22
摘要： Method to generate and select a meganuclease having at least two altered characteristics in comparison to a parent meganuclease, comprising the steps: a. constructing from a parent meganuclease, a first series of variants which differ from said parent meganuclease by at least one acid amino substitution; b. screening the variants from said first series of step a. and selecting those which have a first altered characteristic; c. constructing from the selected variants of step b. a second series of variants having a least one other amino acid substitution; d. screening the variants from said series of step b. and selecting those which have said first altered characteristic and a second altered characteristic. Polypeptide obtained from said method.
摘要翻译：与父母大范围核酸酶相比，产生和选择具有至少两个改变的特征的大范围核酸酶的方法，包括以下步骤：a。从母体大范围核酸酶构建第一系列变体，其不同于所述亲本大范围核酸酶至少一个酸性氨基取代; b。从所述第一系列步骤a筛选变体。并选择具有第一改变特征的那些; C。从步骤b的选定变体构建。具有至少一个其它氨基酸取代的第二系列变体; d。筛选所述系列步骤b中的变体。并选择具有所述第一改变特性和第二改变特性的那些。从所述方法获得的多肽。

17. 发明申请

US20110158974A1 Heterodimeric Meganucleases and Use Thereof 审中-公开
标题翻译：异二聚体大核糖核酸酶及其用途
公开(公告)号：US20110158974A1
公开(公告)日：2011-06-30
申请号：US11908934
申请日：2006-03-15
申请人： Philippe Duchateau , Frederic Paques
发明人： Philippe Duchateau , Frederic Paques
IPC分类号： A61K38/46 , C12N9/16 , C12N15/63 , C12N1/00 , A61P31/12 , A61P43/00 , A01K67/00 , A01H1/00 , C12N15/09
CPC分类号： C12N9/22 , A61K38/00 , A61K48/00
摘要： Heterodimeric meganuclease comprising two domains of different meganucleases which are in two separate polypeptides, said heterodimeric meganuclease being able to cleave a chimeric DNA target sequence comprising one different half of each parent meganuclease DNA target sequence.Use of said herodimeric meganuclease and derived products for genetic engineering, genome therapy and antiviral therapy.
摘要翻译：异源二聚体大范围核酸酶包含两个分离的多肽中的两个不同的大范围核酸酶结构域，所述异源二聚体大范围核酸酶能够切割包含每个亲本大范围核酸酶DNA靶序列的不同一半的嵌合DNA靶序列。使用所述的英雄二聚大范围核酸酶和衍生产品进行遗传工程，基因组治疗和抗病毒治疗。

18. 发明申请

US20110151539A1 USE OF MEGANUCLEASES FOR INDUCING HOMOLOGOUS RECOMBINATION EX VIVO AND IN TOTO IN VERTEBRATE SOMATIC TISSUES AND APPLICATION THEREOF 审中-公开
公开(公告)号：US20110151539A1
公开(公告)日：2011-06-23
申请号：US13021473
申请日：2011-02-04
申请人： Sylvain Arnould , Sylvia Bruneau , Jean-Pierre Cabaniols , Patrick Chames , André Choulika , Philippe Duchateau , Jean-Charles Epinat , Agnes Gouble , Emmanuel Lacroix , Frederic Paques , Christophe Perez-Michaut , Julianne Smith , David Sourdive
发明人： Sylvain Arnould , Sylvia Bruneau , Jean-Pierre Cabaniols , Patrick Chames , André Choulika , Philippe Duchateau , Jean-Charles Epinat , Agnes Gouble , Emmanuel Lacroix , Frederic Paques , Christophe Perez-Michaut , Julianne Smith , David Sourdive
IPC分类号： C12N9/16
CPC分类号： C12N15/907 , A01K67/0275 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/105 , A01K2267/03 , A01K2267/0337 , A61K38/00 , A61K38/1709 , A61K38/465 , A61K48/00 , A61K48/0058 , C07K14/435 , C07K2319/81 , C12N7/00 , C12N9/22 , C12N15/1058 , C12N15/8509 , C12N15/86 , C12N15/90 , C12N2730/10143 , C12N2799/022 , C12N2800/80 , C12N2830/002 , C12N2830/55 , C12N2840/20 , C12N2840/44 , C12Y301/00 , C12Y301/21004
摘要： A single chain homing endonuclease, comprising a first variant of I-CreI having the amino acid sequence of accession number pdb 1g9y and a second variant of I-CreI variant having the amino acid sequence of accession number pdb 1g9y in a single polypeptide.

19. 发明授权

US08530214B2 Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof 有权
标题翻译：使用大范围核酸酶从体外诱导同源重组，并在脊椎动物体细胞中诱导同源重组及其应用
公开(公告)号：US08530214B2
公开(公告)日：2013-09-10
申请号：US13152961
申请日：2011-06-03
申请人： Sylvain Arnould , Patrick Chames , Philippe Duchateau , Jean-Charles Epinat , Emmanuel Lacroix , Frederic Paques
发明人： Sylvain Arnould , Patrick Chames , Philippe Duchateau , Jean-Charles Epinat , Emmanuel Lacroix , Frederic Paques
IPC分类号： C12N15/55 , C12N9/22 , C12N15/00 , C12N15/11
CPC分类号： C12N15/907 , A01K67/0275 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/105 , A01K2267/03 , A01K2267/0337 , A61K38/00 , A61K38/1709 , A61K38/465 , A61K48/00 , A61K48/0058 , C07K14/435 , C07K2319/81 , C12N7/00 , C12N9/22 , C12N15/1058 , C12N15/8509 , C12N15/86 , C12N15/90 , C12N2730/10143 , C12N2799/022 , C12N2800/80 , C12N2830/002 , C12N2830/55 , C12N2840/20 , C12N2840/44 , C12Y301/00 , C12Y301/21004
摘要： The present invention relates to a polynucleotide encoding a monomer of an I-CreI variant said monomer comprising mutations in the amino acid sequence of SEQ ID NO: 34, wherein said mutations include: (i) at least one and up to eleven amino acid substitutions from residue S22 to Q44 said substitutions selected from the group consisting of substitutions at positions I24, Q26, K28, N30, S32, Y33, Q38, S40 and T42; and (ii) at least one and up to six amino acid substitutions from residue Y66 to I77 said substitutions selected from the group consisting of substitutions at positions Y66, R68, R70, V73 and I77; and wherein said monomer when in dimeric form binds and cleaves DNA.
摘要翻译：本发明涉及编码I-CreI变体的单体的多核苷酸，所述单体包含SEQ ID NO：34的氨基酸序列中的突变，其中所述突变包括：（i）至少一个和至多十一个氨基酸取代从残基S22至Q44，所述取代选自I24，Q26，K28，N30，S32，Y33，Q38，S40和T42位置的取代; 和（ii）残基Y66至I77的至少一个和多至六个氨基酸取代，所述取代选自Y66，R68，R70，V73和I77位置的取代; 并且其中当二聚体形式的所述单体结合并切割DNA时。

20. 发明授权

US07842489B2 Use of meganucleases for inducing homologous recombination ex vivo and in toto in vertebrate somatic tissues and application thereof 失效
标题翻译：使用大范围核酸酶从体外诱导同源重组，并在脊椎动物体细胞中诱导同源重组及其应用
公开(公告)号：US07842489B2
公开(公告)日：2010-11-30
申请号：US12710969
申请日：2010-02-23
申请人： Sylvain Arnould , Sylvia Bruneau , Jean-Pierre Cabaniols , Patrick Chames , Andre Choulika , Philippe Duchateau , Jean-Charles Epinat , Agnes Gouble , Emmanuel Lacroix , Frederic Paques , Christophe Perez-Michaut , Julianne Smith , David Sourdive
发明人： Sylvain Arnould , Sylvia Bruneau , Jean-Pierre Cabaniols , Patrick Chames , Andre Choulika , Philippe Duchateau , Jean-Charles Epinat , Agnes Gouble , Emmanuel Lacroix , Frederic Paques , Christophe Perez-Michaut , Julianne Smith , David Sourdive
IPC分类号： C12N9/22 , C12N15/55
CPC分类号： C12N15/907 , A01K67/0275 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/105 , A01K2267/03 , A01K2267/0337 , A61K38/00 , A61K38/1709 , A61K38/465 , A61K48/00 , A61K48/0058 , C07K14/435 , C07K2319/81 , C12N7/00 , C12N9/22 , C12N15/1058 , C12N15/8509 , C12N15/86 , C12N15/90 , C12N2730/10143 , C12N2799/022 , C12N2800/80 , C12N2830/002 , C12N2830/55 , C12N2840/20 , C12N2840/44 , C12Y301/00 , C12Y301/21004
摘要： A single chain homing endonuclease, comprising a first variant of I-CreI having the amino acid sequence of accession number pdb 1g9y (SEQ ID NO: 23 is residues 1-153 of pdb Ig9y) and a second variant of I-CreI variant having the amino acid sequence of accession number pdb 1g9y (SEQ ID NO: 23 is residues 1-153 of pdb Ig9y) in a single polypeptide.
摘要翻译：包含具有登录号pdb 1g9y（SEQ ID NO：23的氨基酸序列为pdb Ig9y的残基1-153）的I-CreI的第一变体的单链归巢内切核酸酶以及I-CreI变体的第二变体，其具有在单个多肽中，登录号pdb 1g9y（SEQ ID NO：23是pdb Ig9y的残基1-153）的氨基酸序列。

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