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11. 发明授权

US08692070B2 Plants with improved nitrogen utilization and stress tolerance 有权
标题翻译：具有改善氮利用和胁迫耐受性的植物
公开(公告)号：US08692070B2
公开(公告)日：2014-04-08
申请号：US12916854
申请日：2010-11-01
申请人： James McLaren , Nicholas Duck , Brian Vande Berg , Philip Hammer , Laura Schouten
发明人： James McLaren , Nicholas Duck , Brian Vande Berg , Philip Hammer , Laura Schouten
IPC分类号： C12N15/82 , C12N15/87 , A01H5/00 , A01H5/10 , C12N1/21 , C12N15/00 , C12N15/29 , C12N15/54 , C07H21/04
CPC分类号： C12N15/8271 , C12N9/1217 , C12N15/8261 , Y02A40/146
摘要： The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, and/or alleviating a limitation such that yield is increased, or a combination of these and that have been transformed using a novel vector construct including a synthetic N-acetyl glutamate kinase (NAGK) gene that modulates nitrogen use in plants. The invention also includes the overexpression and enzymatic characterization of an arginine-insensitive NAGK isolated from a bacterial strain that improves stress tolerance and nitrogen uptake, metabolism or both. In various embodiments, the vector construct includes one or more nucleic acid sequences including SEQ ID NO: 1. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.
摘要翻译：本发明涉及具有增加的氮利用效率，胁迫耐受性和/或减轻增加产量的限制的转基因植物，或者这些和这些的组合已经使用包含合成N-乙酰基的新载体构建体进行转化的转基因植物谷氨酸激酶（NAGK）基因，调节植物中的氮利用。本发明还包括从细菌菌株分离的精氨酸不敏感NAGK的过表达和酶学表征，其改善胁迫耐受性和氮摄取，代谢或两者。在各种实施方案中，载体构建体包括一个或多个包含SEQ ID NO：1的核酸序列。本发明还涉及用于转化植物的分离的载体和用于检测转化植物的抗体。本发明还涉及在植物中表达对应于调节植物中氮利用或由氮条件调节的核酸序列的核酸分子的方法。

12. 发明申请

US20070004907A1 Genes conferring herbicide resistance 有权
标题翻译：赋予除草剂抗性的基因
公开(公告)号：US20070004907A1
公开(公告)日：2007-01-04
申请号：US11517991
申请日：2006-09-08
申请人： Philip Hammer , Nicholas Duck
发明人： Philip Hammer , Nicholas Duck
IPC分类号： C07K14/415
CPC分类号： C12N15/8275
摘要： Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules encoding glyphosate resistance proteins are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or the nucleotide sequence set forth in SEQ ID NO: 1.
摘要翻译：提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。组合物还包括转化的植物，植物细胞，组织和种子。特别地，提供编码抗草甘膦蛋白质的分离的核酸分子。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列的分离的核酸分子。

13. 发明申请

US20070136840A1 GRG23 and GRG 51 genes conferring herbicide resistance 有权
标题翻译：赋予除草剂抗性的GRG23和GRG51基因
公开(公告)号：US20070136840A1
公开(公告)日：2007-06-14
申请号：US11605824
申请日：2006-11-29
申请人： Cheryl Peters , Jill Hinson , Philip Hammer , Brian Vande Berg , Laura Schouten , Brian Carr
发明人： Cheryl Peters , Jill Hinson , Philip Hammer , Brian Vande Berg , Laura Schouten , Brian Carr
IPC分类号： A01H1/00 , C12N15/82 , C12N5/04
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。本发明另外提供了使用荧光底物测量酶动力学活性的方法。

14. 发明申请

US20060277632A1 Methods for production of chitin and chitosan 审中-公开
标题翻译：几丁质和壳聚糖的生产方法
公开(公告)号：US20060277632A1
公开(公告)日：2006-12-07
申请号：US11434526
申请日：2006-05-15
申请人： Brian Carr , Philip Hammer
发明人： Brian Carr , Philip Hammer
IPC分类号： A01H1/00 , C08B37/08 , C07H21/04 , C12P19/28 , C12N1/21 , C12N1/16 , C12N15/74
CPC分类号： C12N9/18 , C08B37/003 , C12N9/1051 , C12N9/1096 , C12N9/80 , C12N15/8246 , C12P19/04 , C12P19/26
摘要： Compositions and methods for producing chitin and chitosan are provided. The compositions comprise genetically modified organisms, including fungi, yeast, bacterial and plant organisms that have been engineered to express heterologous genes involved in chitin and chitosan synthesis. Microorganisms and plants that have been modified for production of chitin and/or chitosan within the vacuole of a cell are encompassed. Methods for production of chitin also comprise culturing the genetically engineered organisms in conditions that allow for chitin production. Further methods include converting the chitin to chitosan by a chemical process. Production of chitosan also comprises culturing organisms that are genetically modified to produce chitosan without the need for chemical modification. Methods for in vitro chitosan production are also encompassed.
摘要翻译：提供了生产壳多糖和壳聚糖的组合物和方法。所述组合物包含经改造的生物体，包括真菌，酵母，细菌和植物生物体，其被工程化以表达参与壳多糖和壳聚糖合成的异源基因。包括在细胞液泡内修饰用于生产壳多糖和/或壳聚糖的微生物和植物。生产甲壳素的方法还包括在允许甲壳素生产的条件下培养基因工程生物体。其他方法包括通过化学方法将几丁质转化为壳聚糖。壳聚糖的生产还包括培养经遗传修饰以生产壳聚糖而不需要化学改性的生物体。还包括体外壳聚糖生产的方法。

15. 发明申请

US20060253921A1 Identification of a new class of EPSP synthases 有权
标题翻译：鉴定一类新的EPSP合成酶
公开(公告)号：US20060253921A1
公开(公告)日：2006-11-09
申请号：US11400598
申请日：2006-04-07
申请人： Nadine Carozzi , Brian Carr , Philip Hammer
发明人： Nadine Carozzi , Brian Carr , Philip Hammer
IPC分类号： C07H21/04 , C12N15/82 , C12N5/04 , A01H1/00
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring tolerance to glyphosate in bacteria, plants, plant cells, tissues and seeds are provided. Compositions include a novel class of EPSPS enzymes, designated Class III, and polynucleotides encoding such enzymes, vectors comprising those polynucleotides, and host cells comprising the vectors. The novel proteins comprise at least one sequence domain selected from the Class III domains provided herein. These sequence domains can be used to identify EPSP synthases with glyphosate resistance activity.
摘要翻译：提供了在细菌，植物，植物细胞，组织和种子中赋予草甘膦耐受性的组合物和方法。组合物包括命名为III类的新一类EPSPS酶和编码这些酶的多核苷酸，包含这些多核苷酸的载体和包含载体的宿主细胞。该新型蛋白质包含至少一个选自本文提供的III类结构域的序列结构域。这些序列结构域可用于鉴定具有草甘膦抗性活性的EPSP合成酶。

16. 发明申请

US20060218662A1 Cis-acting regulatory elements from tripsacum dactyloides 有权
标题翻译：来自tripsacum dactyloides的顺式作用的调节元件
公开(公告)号：US20060218662A1
公开(公告)日：2006-09-28
申请号：US11377318
申请日：2006-03-16
申请人： Philip Hammer
发明人： Philip Hammer
IPC分类号： A01H5/00 , C07H21/04 , C12N15/82 , C12N5/04 , A01H1/00
CPC分类号： C12N15/8216
摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include a novel promoter nucleotide sequence for the gene encoding ubiquitin in Tripsacum dactyloides, as well as vectors, microorganisms, plants and plant cells comprising the promoter nucleotide sequence, or variants and fragments thereof. Methods for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein are also provided. The methods comprise stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
摘要翻译：本发明提供调节植物中异源核苷酸序列表达的组合物和方法。组合物包括用于编码Tripsacum dactyloides中的泛素的基因的新型启动子核苷酸序列，以及包含启动子核苷酸序列或其变体和片段的载体，微生物，植物和植物细胞。还提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。所述方法包括将与本发明的启动子可操作地连接的核苷酸序列稳定地并入植物细胞的基因组中，并再生表达核苷酸序列的稳定转化的植物。

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