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11. 发明授权

US6080851A Ribozymes with linked anchor sequences 失效
标题翻译：具有连锁锚序列的核酶
公开(公告)号：US6080851A
公开(公告)日：2000-06-27
申请号：US448446
申请日：1995-07-10
申请人： Catherine J. Pachuk , Leslie R. Coney , Fred T. Oakes
发明人： Catherine J. Pachuk , Leslie R. Coney , Fred T. Oakes
IPC分类号： C12N15/09 , A61K31/70 , A61P31/12 , C07H21/04 , C12N9/00 , C12N15/113 , C12Q1/68
CPC分类号： C12N15/113 , C12N15/1135 , C07K2319/00 , C12N2310/111 , C12N2310/121 , C12N2310/315 , C12N2310/351 , C12N2310/353 , C12N2310/53
摘要： Ribozymes are provided in which the ribozymes have a catalytic sequence, two legs and at least one anchor sequence complementary to the substrate mRNA at a location noncontiguous with the portions of the substrate mRNA complementary to the legs. In certain preferred embodiments, ribozymes capable of cleaving the L6 mRNA or both the L6 and K28 mRNAs are provided. Methods are provided for the treatment of chronic myelogenous leukemia (CML) and acute lymphoblastic leukemia (ALL) patients having the L6 and K28 translocations and, therefore, the L6 mRNA. Additionally, combination treatments comprising components specific for both the K28 and L6 mRNAs are provided. Combination treatments are also provided for those patients which express c-myc in combination with K28 and/or L6 mRNAs. Methods of treatment include, for example, administration of ribozymes and antisense oligonucleotides directed against these mRNAs.
摘要翻译： PCT No.PCT / US93 / 11144 Sec。 371日期：1995年7月10日 102（e）日期1995年7月10日PCT提交1993年11月16日PCT公布。出版物WO94 / 13793 日期1994年6月23日提供了这样的酶，其中核酶具有催化序列，两条腿和与底物mRNA互补的位置与底物mRNA互补的至少一个锚定序列。在某些优选实施方案中，提供能够切割L6 mRNA或L6和K28 mRNA两者的核酶。提供了用于治疗慢性骨髓性白血病（CML）和具有L6和K28易位的急性淋巴细胞性白血病（ALL）患者以及L6mRNA的方法。另外，提供包含对K28和L6 mRNA特异性成分的组合处理。还为那些表达c-myc与K28和/或L6mRNA组合的患者提供组合治疗。治疗方法包括例如施用针对这些mRNA的核酶和反义寡核苷酸。

12. 发明授权

US5514550A Nucleic acid test article and its use to detect a predetermined nucleic acid 失效
标题翻译：核酸测试制品及其用于检测预定核酸的用途
公开(公告)号：US5514550A
公开(公告)日：1996-05-07
申请号：US300297
申请日：1994-09-02
申请人： John B. Findlay , Janice M. Mayer , Marlene M. King , Fred T. Oakes , Chu-an Chang , Corey H. Levenson
发明人： John B. Findlay , Janice M. Mayer , Marlene M. King , Fred T. Oakes , Chu-an Chang , Corey H. Levenson
IPC分类号： C12Q1/68 , C12Q1/70 , C12P19/34
CPC分类号： C12Q1/703 , C12Q1/6834 , C12Q1/6876
摘要： A nucleic acid test article can be used to detect a targeted nucleic acid found in a specimen. The test article includes a substrate having two opposing surfaces and a water-insoluble nucleic acid probe attached in a distinct zone of one of the surfaces. The probe is prepared from a water-insoluble particle to which is covalently attached an oligonucleotide which is complementary to the targeted nucleic acid. Substantially none of the probe is embedded within the surface of the substrate. Particularly useful test articles have a multiplicity of water-insoluble probes located in distinct zones on one of the substrate surfaces. These probes are useful for the detection of a multiplicity of targeted nucleic acids, particularly after amplification by polymerase chain reaction.
摘要翻译：核酸测试制品可用于检测样品中发现的靶向核酸。测试制品包括具有两个相对表面的底物和附着在一个表面的不同区域中的水不溶性核酸探针。该探针由水不溶性颗粒制备，其中共价连接与靶向核酸互补的寡核苷酸。基本上没有一个探针嵌入基片表面。特别有用的测试制品具有位于其中一个衬底表面上的不同区域中的多个水不溶性探针。这些探针可用于检测多种靶向核酸，特别是通过聚合酶链反应扩增后。

13. 发明授权

US5272077A Method for preparing a covalent conjugate of an oligonucleotide and an enzyme 失效
标题翻译：制备寡核苷酸和酶的共价缀合物的方法
公开(公告)号：US5272077A
公开(公告)日：1993-12-21
申请号：US58723
申请日：1993-05-10
申请人： Harold C. Warren, III , Fred T. Oakes
发明人： Harold C. Warren, III , Fred T. Oakes
IPC分类号： C07H21/00 , C12N9/96 , C12N9/04 , C12N9/06 , C12N9/08
CPC分类号： C07H21/00
摘要： A method is provided for preparing a covalent conjugate of an oligonucleotide and an enzyme, such as peroxidase. This conjugate can be used as a probe in hybridization assays and in polymerase chain reaction procedures. The method generally comprises the steps of: reacting an enzyme having a reactive amino group with a mercapto-substituted organic compound to form a blocked intermediate, removing the blocking group to form an enzyme reagent, and reacting the enzyme reagent with a functionalized oligonucleotide reagent.
摘要翻译：提供了制备寡核苷酸和酶如过氧化物酶的共价缀合物的方法。该缀合物可用作杂交测定和聚合酶链反应程序中的探针。该方法通常包括以下步骤：使具有反应性氨基的酶与巯基取代的有机化合物反应形成封闭的中间体，除去封闭基团以形成酶试剂，并使酶试剂与官能化的寡核苷酸试剂反应。

14. 发明授权

US5254469A Oligonucleotide-enzyme conjugate that can be used as a probe in hybridization assays and polymerase chain reaction procedures 失效
标题翻译：可用于杂交测定和聚合酶链反应程序中的探针的寡核苷酸 - 酶结合物
公开(公告)号：US5254469A
公开(公告)日：1993-10-19
申请号：US762136
申请日：1991-09-17
申请人： Harold C. Warren, III , Fred T. Oakes
发明人： Harold C. Warren, III , Fred T. Oakes
IPC分类号： C07H21/00 , C12N9/96 , C12N9/04 , C12N9/06 , C12N9/08
CPC分类号： C07H21/00
摘要： A covalent conjugate of an enzyme, such as peroxidase, glucose oxidase, alkaline phosphatase and beta-galactosidase, and an oligonucleotide is herein disclosed. This conjugate can be used as a probe in hybridization assays and in polymerase chain reaction procedures.
摘要翻译：本文公开了酶的共价缀合物，例如过氧化物酶，葡萄糖氧化酶，碱性磷酸酶和β-半乳糖苷酶和寡核苷酸。该缀合物可用作杂交测定和聚合酶链反应程序中的探针。

15. 发明授权

US5154887A Phenalenimine fluorescent dyes and their use in analytical compositions, elements and methods 失效
标题翻译：苯亚胺荧光染料及其在分析组合物，元素和方法中的应用
公开(公告)号：US5154887A
公开(公告)日：1992-10-13
申请号：US705874
申请日：1991-05-28
申请人： Bruce E. Babb , Fred T. Oakes
发明人： Bruce E. Babb , Fred T. Oakes
IPC分类号： C09B55/00 , G01N33/533
CPC分类号： G01N33/533 , C09B55/009 , Y10S435/805
摘要： Certain phenalenimine fluorescent compounds represented by the structure ##STR1## wherein R' and R" are independently hydrogen, alkyl, cycloalkyl, aryl or a heterocycle, or R' comprises the carbon and heteroatoms which form a fused ring with the compound nucleus, are useful in biomedical and analytical determinations. These compounds can be used for staining cells, as well as for the determination of various analytes found in human or animal biological fluids. Such determinations can be carried out in solution or by using dry analytical elements. The fluorescent compounds can be reacted with quinone nuclei to form reducible compounds which are also useful in analytical methods. In addition, the compounds can be incorporated into what are known as "loadable" latex particles to form detectable labels and biological reagents.
摘要翻译：由结构表示的某些吩噻嗪类荧光化合物，其中R'和R“独立地是氢，烷基，环烷基，芳基或杂环，或R'包括与化合物核形成稠合环的碳和杂原子可用于生物医学和分析测定。这些化合物可用于染色细胞，以及用于测定在人或动物生物流体中发现的各种分析物。这种测定可以在溶液中或通过使用干燥的分析元件进行。荧光化合物可与醌核反应形成可还原的化合物，也可用于分析方法。此外，化合物可以掺入所谓的“可装载”胶乳颗粒中以形成可检测的标记物和生物试剂。

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