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11. 发明授权

US08445247B2 Stabilization of perhydrolases 有权
标题翻译：过水解酶的稳定化
公开(公告)号：US08445247B2
公开(公告)日：2013-05-21
申请号：US13547583
申请日：2012-07-12
申请人： Robert DiCosimo , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
发明人： Robert DiCosimo , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
IPC分类号： C12P7/40 , C12N9/00 , C12N9/96 , D06L1/02 , C11D3/386 , C07H21/04
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein are enzyme powders comprising a spray-dried formulation of at least one CE-7 esterase, at least one oligosaccharide excipient, and optionally at least one surfactant. Also disclosed herein is a process for producing peroxycarboxylic acids from carboxylic acid esters using the aforementioned enzyme powders. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
摘要翻译：本文公开了包含至少一种CE-7酯酶，至少一种寡糖赋形剂和任选的至少一种表面活性剂的喷雾干燥制剂的酶粉末。本文还公开了使用上述酶粉末从羧酸酯制备过氧羧酸的方法。此外，提供包含通过本文所述方法生产的过酸的消毒剂和衣物护理制剂。

12. 发明申请

US20110300121A1 Stabilization Of Perhydrolases 有权
标题翻译：过水解酶的稳定
公开(公告)号：US20110300121A1
公开(公告)日：2011-12-08
申请号：US13213583
申请日：2011-08-19
申请人： Robert DICOSIMO , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
发明人： Robert DICOSIMO , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
IPC分类号： A01N63/02 , A01P1/00 , C11D3/60 , C12N9/96
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein is a method for stabilization of the perhydrolase activity of the CE-7 esterase in a formulation with a carboxylic acid ester that employs the addition of a buffering agent, substantially undissolved, to the mixture of the CE-7 esterase and the carboxylic acid ester. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
摘要翻译：本文公开了一种用于在具有羧酸酯的制剂中稳定CE-7酯酶的过水解酶活性的方法，其使用向CE-7酯酶和羧酸的混合物中添加基本上未溶解的缓冲剂酯。此外，提供包含通过本文所述方法生产的过酸的消毒剂和衣物护理制剂。

13. 发明授权

US07695945B2 Immobilized microbial nitrilase for production of glycolic acid 有权
标题翻译：用于生产乙醇酸的固定化微生物腈水解酶
公开(公告)号：US07695945B2
公开(公告)日：2010-04-13
申请号：US11930550
申请日：2007-10-31
申请人： Robert Dicosimo , Arie Ben-Bassat
发明人： Robert Dicosimo , Arie Ben-Bassat
IPC分类号： C12N9/96 , C12P21/06 , C12N9/00 , C12N9/78 , C07H21/04
CPC分类号： C12N9/78 , C12N11/06 , C12N11/10 , C12P7/42 , Y02P20/584 , Y02P20/588
摘要： The present invention provides a process for preparing an enzyme catalyst having nitrilase activity for hydrolysis of glycolonitrile to glycolic acid with improved retention of recovered catalyst activity in consecutive batch reactions with catalyst recycle, said process comprising pretreating the enzyme catalyst with glutaraldehyde. The glutaraldehyde-pretreated enzyme catalyst has improved specific activity when compared to non-glutaraldehyde-pretreated enzyme catalysts, and thereby, has improved overall catalyst activity and productivity.
摘要翻译：本发明提供一种制备具有腈水解酶活性的酶催化剂的方法，所述酶催化剂具有乙醇腈水解为乙醇酸的酶催化剂，其具有在催化剂再循环的连续间歇反应中具有回收的催化剂活性的保持性，所述方法包括用戊二醛预处理酶催化剂。与非戊二醛预处理的酶催化剂相比，戊二醛预处理的酶催化剂具有改善的比活性，从而改善了总的催化剂活性和生产率。

14. 发明申请

US20100086621A1 MULTI-COMPONENT PERACID GENERATION SYSTEM 有权
标题翻译：多组分PERACID生成系统
公开(公告)号：US20100086621A1
公开(公告)日：2010-04-08
申请号：US12572107
申请日：2009-10-01
申请人： Robert DiCosimo , Arie Ben-Bassat , William R. Cahill , David George Dipietro , Eugenia Costa Hann , Mark S. Payne , Richard Alan Reynolds , Raymond Richard Zolandz
发明人： Robert DiCosimo , Arie Ben-Bassat , William R. Cahill , David George Dipietro , Eugenia Costa Hann , Mark S. Payne , Richard Alan Reynolds , Raymond Richard Zolandz
IPC分类号： A01N59/00 , A01N37/00 , A01P1/00 , C01B15/00 , C11D3/39
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein are multi-component peroxycarboxylic acid generation systems for enzymatically producing aqueous formulations of peroxycarboxylic acids suitable for use in, e.g., disinfectant and/or bleaching applications. The multi-component peroxycarboxylic acid generation systems comprise at least one carbohydrate esterase family 7 enzyme having perhydrolytic activity.
摘要翻译：本文公开了用于酶促生产适用于例如消毒剂和/或漂白应用的过氧羧酸的水性制剂的多组分过氧羧酸生成系统。多组分过氧羧酸生成系统包含至少一种具有过水解活性的碳水化合物酯酶家族7酶。

15. 发明申请

US20090111158A1 IMMOBILIZED MICROBIAL NITRILASE FOR PRODUCTION OF GLYCOLIC ACID 有权
标题翻译：用于生产甘油酸的稳定的微生物硝酸盐
公开(公告)号：US20090111158A1
公开(公告)日：2009-04-30
申请号：US11930550
申请日：2007-10-31
申请人： Robert Dicosimo , Arie Ben-Bassat
发明人： Robert Dicosimo , Arie Ben-Bassat
IPC分类号： C12N9/96
CPC分类号： C12N9/78 , C12N11/06 , C12N11/10 , C12P7/42 , Y02P20/584 , Y02P20/588
摘要： The present invention provides a process for preparing an enzyme catalyst having nitrilase activity for hydrolysis of glycolonitrile to glycolic acid with improved retention of recovered catalyst activity in consecutive batch reactions with catalyst recycle, said process comprising pretreating the enzyme catalyst with glutaraldehyde. The glutaraldehyde-pretreated enzyme catalyst has improved specific activity when compared to non-glutaraldehyde-pretreated enzyme catalysts, and thereby, has improved overall catalyst activity and productivity.
摘要翻译：本发明提供一种制备具有腈水解酶活性的酶催化剂的方法，所述酶催化剂具有乙醇腈水解为乙醇酸的酶催化剂，其具有在催化剂再循环的连续间歇反应中具有回收的催化剂活性的保持性，所述方法包括用戊二醛预处理酶催化剂。与非戊二醛预处理的酶催化剂相比，戊二醛预处理的酶催化剂具有改善的比活性，从而改善了总的催化剂活性和生产率。

16. 发明授权

US07378261B2 Method for preparing para-hydroxystyrene by biocatalytic decarboxylation of para-hydroxycinnamic acid in a biphasic reaction medium 失效
标题翻译：在双相反应介质中通过对羟基肉桂酸的生物催化脱羧制备对羟基苯乙烯的方法
公开(公告)号：US07378261B2
公开(公告)日：2008-05-27
申请号：US10824581
申请日：2004-04-14
申请人： Arie Ben-Bassat , Sharon L. Haynie , David J. Lowe , Lisa L. Huang
发明人： Arie Ben-Bassat , Sharon L. Haynie , David J. Lowe , Lisa L. Huang
IPC分类号： C12P7/22 , C12N9/88 , C12N5/10 , C12N1/21 , C12N15/00 , C12N11/00 , C12P21/06
CPC分类号： C12P7/22 , C12P7/42
摘要： A biocatalytic method for preparing para-hydroxystyrene from para-hydroxycinnamic acid is described. The method uses an enzyme source having para-hydroxycinnamic acid decarboxylase activity to catalyze the decarboxylation of para-hydroxycinnamic acid in a biphasic reaction medium to produce para-hydroxystyrene, which is extracted into the organic phase of the biphasic reaction medium. The method results in a high yield of para-hydroxystyrene due to the decreased exposure of the enzyme source to the inhibitory product. The product is readily recovered from the extractant, or may be chemically derivatized directly in the extractant before recovery.
摘要翻译：描述了由对羟基肉桂酸制备对羟基苯乙烯的生物催化方法。该方法使用具有对羟基肉桂酸脱羧酶活性的酶源来催化双相反应介质中对羟基肉桂酸的脱羧作用，产生对羟基苯乙烯，将其提取到双相反应介质的有机相中。该方法由于酶源暴露于抑制产物而导致对羟基苯乙烯的高产率。该产物容易从萃取剂中回收，或者可以在回收之前在萃取剂中直接化学衍生化。

17. 发明申请

US20050260724A1 Methods for the preparation of para-hydroxycinnamic acid and cinnamic acid at alkaline PH 有权
标题翻译：在碱性PH下制备对羟基肉桂酸和肉桂酸的方法
公开(公告)号：US20050260724A1
公开(公告)日：2005-11-24
申请号：US11105259
申请日：2005-04-13
申请人： Arie Ben-Bassat , Fateme Sariaslani , Lisa Huang , Ranjan Patnaik , David Lowe
发明人： Arie Ben-Bassat , Fateme Sariaslani , Lisa Huang , Ranjan Patnaik , David Lowe
IPC分类号： C12N1/21 , C12P7/40 , C12P7/42 , C12P13/22
CPC分类号： C12P7/42 , C12P7/40
摘要： Methods for the microbial production of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) are provided. Microbes producing either tyrosine or phenylalanine are grown in the presence of either tyrosine ammonium lyase or phenylalanine ammonium lyase respectively where some part of the fermentation is accomplished at alkaline pH. The process results in greater yields and higher rates of para-hydroxycinnamic acid (pHCA) and cinnamic acid (CA) production as compared with fermentation exclusively at physiological pH.
摘要翻译：提供了对羟基肉桂酸（pHCA）和肉桂酸（CA）的微生物生产方法。生产酪氨酸或苯丙氨酸的微生物分别在酪氨酸裂解酶或苯丙氨酸铵裂解酶的存在下生长，其中部分发酵在碱性pH下完成。与仅在生理pH下的发酵相比，该方法产生较高的产率和较高的对羟基肉桂酸（PHCA）和肉桂酸（CA）产率。

18. 发明授权

US06677149B2 Method for stabilizing nitrilase activity and preserving microbial cells with carbamate salts 有权
标题翻译：稳定腈水解酶活性和用氨基甲酸盐保存微生物细胞的方法
公开(公告)号：US06677149B2
公开(公告)日：2004-01-13
申请号：US09854498
申请日：2001-05-14
申请人： Robert Dicosimo , Arie Ben-Bassat , Robert D. Fallon
发明人： Robert Dicosimo , Arie Ben-Bassat , Robert D. Fallon
IPC分类号： C12N104
CPC分类号： C12N1/04 , C12N9/78 , C12N9/96 , C12N11/04 , C12N11/08 , C12N11/10 , C12P13/00
摘要： A method for preserving immobilized or unimmobilized microbial cells having nitrilase activity and for stabilizing the nitrilase activity of unimmobilized or immobilized microbial cells has been developed. Aqueous suspensions containing at least 100 mM bicarbonate, carbonate, or carbamate salts limit microbial contamination of the stored enzyme catalyst, as well as stabilize the desired nitrilase activity of the unimmobilized or immobilized cells. Microorganisms which are characterized by an nitrilase activity and are stabilized and preserved by this method include Acidovorax facilis 72-PF-15 (ATCC 55747), Acidovorax facilis 72-PF-17 (ATCC 55745), Acidovorax facilis 72W (ATCC 55746), and transformed microbial cells having nitrilase activity, the host cells transformed with Acidovorax facilis 72W nitrilase activity. Especially preferred is an embodiment using ammonium carbamate as the inorganic salt.
摘要翻译：已经开发了用于保存具有腈水解酶活性的固定或未固化的微生物细胞并稳定未固定或固定的微生物细胞的腈水解酶活性的方法。含有至少100mM碳酸氢盐，碳酸盐或氨基甲酸盐的含水悬浮液限制所储存的酶催化剂的微生物污染物，以及稳定未固化或固定的细胞的所需腈水解酶活性。其特征在于腈水解酶活性并通过该方法稳定和保存的微生物包括Acidovorax facilis 72-PF-15（ATCC 55747），Acidovorax facilis 72-PF-17（ATCC 55745），Acidovorax facilis 72W（ATCC 55746）和转化的具有腈水解酶活性的微生物细胞，宿主细胞用Acidovorax facilis 72W腈水解酶活性转化。特别优选的是使用氨基甲酸铵作为无机盐的实施方案。

19. 发明授权

US08586339B2 Facilitated process for purification of proteins 有权
标题翻译：促进蛋白质纯化的方法
公开(公告)号：US08586339B2
公开(公告)日：2013-11-19
申请号：US13070118
申请日：2011-03-23
申请人： Robert Dicosimo , Arie Ben-Bassat , Raymond Richard Zolandz
发明人： Robert Dicosimo , Arie Ben-Bassat , Raymond Richard Zolandz
IPC分类号： C12P7/24 , C12N9/00 , C12N9/10 , C12N9/16 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12N9/18 , C12Y301/01072
摘要： A two-stage heat treatment process is provided to improve the processability of recombinant microbial biomass comprising an enzyme having perhydrolytic activity. More specifically, a process is provided to treat the recombinant microbial biomass comprising a Thermotoga sp. acetyl xylan esterase having perhydrolytic activity such that microfiltration may be used to partially-purify and/or concentrate protein preparations. The acetyl xylan esterase may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, and paper pulp processing applications.
摘要翻译：提供两阶段热处理方法以改善包含具有过水解活性的酶的重组微生物生物质的加工性能。更具体地，提供了一种处理包含Thermotoga sp。的重组微生物生物质的方法。具有过水解活性的乙酰木聚糖酯酶，使得微量过滤可用于部分纯化和/或浓缩蛋白质制剂。乙酰木聚糖酯酶可用于制备适用于各种应用的过氧羧酸，例如清洁，消毒，消毒，漂白，木浆加工和纸浆加工应用。

20. 发明申请

US20120276609A1 Stabilization Of Perhydrolases 有权
标题翻译：过水解酶的稳定
公开(公告)号：US20120276609A1
公开(公告)日：2012-11-01
申请号：US13547583
申请日：2012-07-12
申请人： Robert DICOSIMO , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
发明人： Robert DICOSIMO , Arie Ben-Bassat , Mark S. Payne , Raymond Richard Zolandz
IPC分类号： C12N9/96
CPC分类号： C12N9/18 , A61L2/183 , A61L2/186 , A61L2/22 , A61L2202/24 , C11D3/06 , C11D3/10 , C11D3/201 , C11D3/2017 , C11D3/2044 , C11D3/2048 , C11D3/2068 , C11D3/2079 , C11D3/2082 , C11D3/2086 , C11D3/2093 , C11D3/221 , C11D3/226 , C11D3/32 , C11D3/361 , C11D3/38636 , C11D3/3947 , C11D17/041 , C12P7/40 , C12Y301/01001 , Y02E50/16
摘要： Disclosed herein are enzyme powders comprising a spray-dried formulation of at least one CE-7 esterase, at least one oligosaccharide excipient, and optionally at least one surfactant. Also disclosed herein is a process for producing peroxycarboxylic acids from carboxylic acid esters using the aforementioned enzyme powders. Further, disinfectant and laundry care formulations comprising the peracids produced by the processes described herein are provided.
摘要翻译：本文公开了包含至少一种CE-7酯酶，至少一种寡糖赋形剂和任选的至少一种表面活性剂的喷雾干燥制剂的酶粉末。本文还公开了使用上述酶粉末从羧酸酯制备过氧羧酸的方法。此外，提供包含通过本文所述方法生产的过酸的消毒剂和衣物护理制剂。

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