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11. 发明申请

US20090053725A1 Using DNA aptamers and quantum dots for the detection of proteins or other targets 有权
标题翻译：使用DNA适体和量子点检测蛋白质或其他靶标
公开(公告)号：US20090053725A1
公开(公告)日：2009-02-26
申请号：US12217833
申请日：2008-07-09
申请人： Eric A. Holwitt , Jonathan L. Kiel , Veronica Franz Sorola
发明人： Eric A. Holwitt , Jonathan L. Kiel , Veronica Franz Sorola
IPC分类号： C12Q1/68 , C12M1/00
CPC分类号： C12Q1/6818 , C12Q1/6825 , C12Q2525/205 , C12Q2563/155 , C12Q2565/101
摘要： The solutions provided here use DNA aptamers and quantum dots for the detection of bacteria, viruses, proteins or other targets. An example of a method described here comprises: providing a complex of DNA complementary strands, one strand being an aptamer, having one strand covalently linked to a quantum dot, and having the other strand linked to a quencher; and contacting said complex of DNA complementary strands with a microorganism or components thereof, under conditions that permit binding of said aptamer with said microorganism or components thereof. In some examples described here, the methods and systems are extremely simple to use and appear to have several advantages over the traditional ELISA. Since no blocking steps are required and the number of washing steps is reduced, the time required to conduct the test is greatly reduced. In some examples described here, a quantum dot aptamer complex comprises one strand of a duplex DNA molecule linked to the quantum dot by an amide bond. It does not matter if the aptamer or the complimentary strand is attached. However, the strand that is not attached contains a non-radiative quencher. Upon addition of the aptamers' target, the amount of light emitted by the quantum dots increases. In some examples described here, the methods and systems can also be used in reverse, with the aptamers' target immobilized on a microtiter plate. This permits an assay like a competitive immuno-assay.
摘要翻译：这里提供的解决方案使用DNA适体和量子点来检测细菌，病毒，蛋白质或其他靶标。本文描述的方法的实例包括：提供DNA互补链的复合物，一条链是适体，具有共价连接到量子点的一条链，并且另一条链与猝灭剂连接; 以及在允许所述适体与所述微生物或其组分结合的条件下，将DNA互补链的所述复合物与微生物或其组分接触。在这里描述的一些实例中，所述方法和系统使用非常简单，并且似乎具有优于传统ELISA的几个优点。由于不需要阻塞步骤并且减少洗涤步骤的数量，所以进行测试所需的时间大大降低。在这里描述的一些实例中，量子点适体复合物包含通过酰胺键与量子点连接的双链DNA分子的一条链。连接适体或互补链无关紧要。然而，未连接的链含有非辐射猝灭剂。当添加适体的目标时，量子点发射的光量增加。在这里描述的一些实例中，方法和系统也可以相反地使用，将适体的靶固定在微量滴定板上。这允许像竞争性免疫测定那样的测定。

12. 发明授权

US08790877B2 Using DNA aptamers and quantum dots for the detection of proteins or other targets 有权
标题翻译：使用DNA适体和量子点检测蛋白质或其他靶标
公开(公告)号：US08790877B2
公开(公告)日：2014-07-29
申请号：US12217833
申请日：2008-07-09
申请人： Eric A. Holwitt , Jonathan L. Kiel , Veronica Franz Sorola
发明人： Eric A. Holwitt , Jonathan L. Kiel , Veronica Franz Sorola
IPC分类号： C12Q1/68 , C07H21/02
CPC分类号： C12Q1/6818 , C12Q1/6825 , C12Q2525/205 , C12Q2563/155 , C12Q2565/101
摘要： The solutions provided here use DNA aptamers and quantum dots for the detection of bacteria, viruses, proteins or other targets. An example of a method described here comprises: providing a complex of DNA complementary strands, one strand being an aptamer, having one strand covalently linked to a quantum dot, and having the other strand linked to a quencher; and contacting the complex of DNA complementary strands with a microorganism or components thereof, under conditions that permit binding of the aptamer with the microorganism or components thereof.
摘要翻译：这里提供的解决方案使用DNA适体和量子点来检测细菌，病毒，蛋白质或其他靶标。本文描述的方法的实例包括：提供DNA互补链的复合物，一条链是适体，具有共价连接到量子点的一条链，并且另一条链与猝灭剂连接; 并在允许适配体与微生物或其组分结合的条件下使DNA互补链的复合物与微生物或其组分接触。

13. 发明授权

US06797822B1 Compositions and methods of use of 8-nitroguanine 有权
标题翻译： 8-硝基鸟嘌呤的组成和使用方法
公开(公告)号：US06797822B1
公开(公告)日：2004-09-28
申请号：US10008803
申请日：2001-11-13
申请人： Eric A. Holwitt
发明人： Eric A. Holwitt
IPC分类号： C07D47300
CPC分类号： G01N33/5091
摘要： Novel methods for the synthesis of 8-nitroguanine are provided. Compositions comprising 8-nitroguanine, made by the novel synthetic methods are also provided herein. Methods of use of 8-nitroguanine, made by the novel synthetic methods, as a standard for detection of 8-nitroguanine in samples are also encompassed within the scope of the present invention. The present invention further concerns methods of predicting organ transplant rejection and detecting exposure to environmental stressors, such as ionizing radiation, toxic chemicals or infectious agents, by detecting 8-nitroguanine in one or more samples from a transplant recipient or an organism exposed to stress.
摘要翻译：提供了用于合成8-硝基鸟嘌呤的新方法。本文还提供了由新型合成方法制备的包含8-硝基鸟嘌呤的组合物。通过新型合成方法制备的8-硝基鸟嘌呤的方法作为检测样品中8-硝基鸟嘌呤的标准也包括在本发明的范围内。本发明还涉及通过检测来自移植受体或暴露于应激的生物体的一种或多种样品中的8-硝基鸟嘌呤来预测器官移植排斥和检测暴露于环境胁迫因子如电离辐射，有毒化学物质或感染因子的方法。

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