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11. 发明授权

US06337100B1 Sunflower seeds with enhanced saturated fatty acid contents 有权
标题翻译：具有增强的饱和脂肪酸含量的向日葵种子
公开(公告)号：US06337100B1
公开(公告)日：2002-01-08
申请号：US09586959
申请日：2000-06-05
申请人： Dennis L. Bidney , Sean Coughlan , Craig Hastings , Christopher J. Scelonge , Lijuan Wang
发明人： Dennis L. Bidney , Sean Coughlan , Craig Hastings , Christopher J. Scelonge , Lijuan Wang
IPC分类号： A23D700
CPC分类号： C12N9/0083 , C12N15/8247
摘要： Antisense expression of a stearoyl-ACP desaturase gene in sunflower results in more than a four-fold increase in seed stearate, and can enhance palmitate content as well. Thus, sunflower oil containing over 40 percent saturated fatty acids can be produced, which is desirable in the context of various products, such as coating fat, margarine, soap and shortening.
摘要翻译：向日葵中的硬脂酰-ACP去饱和酶基因的反义表达导致硬脂酸硬脂酸酯的增加超过4倍，并且还可以提高棕榈酸酯含量。因此，可以生产含有超过40％的饱和脂肪酸的向日葵油，这在各种产品的情况下是可取的，例如包衣脂肪，人造奶油，肥皂和起酥油。

12. 发明申请

US20070143881A1 Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange 审中-公开
标题翻译：提高位点特异性多核苷酸交换效率的方法和组合
公开(公告)号：US20070143881A1
公开(公告)日：2007-06-21
申请号：US11427947
申请日：2006-06-30
申请人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu
发明人： Dennis L. Bidney , Sheila E. Maddock , Xinli E. Wu
IPC分类号： A01H1/00
CPC分类号： C12N15/8213 , C12N15/8205 , C12N15/8207
摘要： Methods and compositions using a site-specific integration system are combined with methods and compositions which deliver compositions via microinjection directly to the embryo sac of a plant. The methods allow for various components of the site-specific recombination system to be introduced into the cellular environment of the embryo sac a composition comprising at least one component of the site-specific recombination system is injected into an embryo sac, providing improved efficiency of expression, recombination, integration, exchange, excision and/or inversion of a polynucleotide of interest. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and tissues, plant parts, and/or plants produced therefrom. Cells, egg cells, zygotes, embryos, endosperm, tissues, seeds, and/or plants produced by the methods and comprising the polynucleotide(s) of interest are also provided.
摘要翻译：使用位点特异性整合系统的方法和组合物与通过显微注射将组合物直接递送至植物胚囊的方法和组合物组合。所述方法允许将位点特异性重组系统的各种组分引入胚囊的细胞环境中，将包含位点特异性重组系统的至少一个组分的组合物注入胚囊，提供更好的表达效率，重组，整合，交换，切除和/或转化感兴趣的多核苷酸。感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳以及由其产生的组织，植物部分和/或植物的基因组中。还提供了通过方法产生并包含感兴趣的多核苷酸的细胞，卵细胞，合子，胚胎，胚乳，组织，种子和/或植物。

13. 发明授权

US06709865B1 Sunflower LOX polynucleotides and related compositions 失效
标题翻译：向日葵LOX多核苷酸和相关组合物
公开(公告)号：US06709865B1
公开(公告)日：2004-03-23
申请号：US09714767
申请日：2000-11-16
申请人： Dennis L. Bidney , Jonathan P. Duvick , Carol Hendrick , Xu Hu , Guihua Lu , Oswald R. Crasta
发明人： Dennis L. Bidney , Jonathan P. Duvick , Carol Hendrick , Xu Hu , Guihua Lu , Oswald R. Crasta
IPC分类号： C12N500
CPC分类号： C12N9/0073 , C07K14/415 , C12N9/0006 , C12N15/8216 , C12N15/8237 , C12N15/8239 , C12N15/8261 , C12N15/8282 , Y02A40/146
摘要： Methods and compositions for modulating development and defense response are provided. Nucleotide sequences encoding a LOX protein are provided. Nucleotide sequences comprising the LOX promoter are also provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and the plant defense response. Transformed plants, plant cells, tissues, and seed are also provided.
摘要翻译：提供了调节发育和防御反应的方法和组合物。提供了编码LOX蛋白的核苷酸序列。还提供了包含LOX启动子的核苷酸序列。序列可用于表达盒中用于调节发育，发育途径和植物防御反应。还提供了转化的植物，植物细胞，组织和种子。

14. 发明授权

US06380461B1 Production of pathogen resistant plants 有权
公开(公告)号：US06380461B1
公开(公告)日：2002-04-30
申请号：US09525258
申请日：2000-03-14
申请人： Dennis L. Bidney , David G. Charne , Glenn S. Cole , Mark K. Mancl , Igor Falak , Katherine A. P. Nazarian , Christopher J. Scelonge
发明人： Dennis L. Bidney , David G. Charne , Glenn S. Cole , Mark K. Mancl , Igor Falak , Katherine A. P. Nazarian , Christopher J. Scelonge
IPC分类号： A01H500
CPC分类号： C12N9/0008 , C12N9/0006 , C12N9/88 , C12N15/8271 , C12N15/8282
摘要： Methods for increasing resistance in plants to pathogens by the expression of a hydrogen peroxide/reactive oxygen species producing enzyme or an oxalate degrading enzyme. The present invention relates to a method of producing a pathogen resistant hybrid plant by crossing the appropriate transgenic expressing a hydrogen peroxide/reactive oxygen species producing enzyme or an oxalate degrading enzyme with pathogen tolerant lines or inbreds obtained through conventional genetic manipulations, or by transformation of tolerant plants or plant tissues with a hydrogen peroxide/reactive oxygen species producing gene or by altering the expression of an endogenase hydrogen peroxide/reactive oxygen species producing gene. The synergistic effect of expression of a hydrogen peroxide/reactive oxygen species producing enzyme or an oxalate degrading enzyme in a tolerant background gives significant and unexpectedly high resistance to the pathogens.

15. 发明授权

US06265638B1 Method of plant transformation 有权
标题翻译：植物转化方法
公开(公告)号：US06265638B1
公开(公告)日：2001-07-24
申请号：US09407574
申请日：1999-09-28
申请人： Dennis L. Bidney , Christopher Jay Scelonge
发明人： Dennis L. Bidney , Christopher Jay Scelonge
IPC分类号： C12N1582
CPC分类号： C12N15/8241 , C12N15/8205 , C12N15/8209
摘要： Compositions and methods for the efficient co-transformation of a plant are provided. Novel compositions are Agrobacterium strains that have been engineered to comprise at least two binary vector plasmids in addition to a helper plasmid comprising the vir functions. Each of the binary vectors comprises its own T-DNA borders flanking a heterologous nucleotide sequence of interest. Methods of the invention comprise the use of these novel multiple-binary vector Agrobacterium strains to co-transform a plant. In this manner, heterologous nucleotide sequences of interest residing on different binary vectors can be independently introduced into the plant in a single transformation event and incorporated in the plant's nuclear DNA in an unlinked manner. The invention also provides for regenerated, fertile transgenic plants, transgenic seeds produced therefrom, and T1 and subsequent generations.
摘要翻译：提供了植物有效共同转化的组合物和方法。除了包含vir功能的辅助质粒之外，新型组合物是经过工程改造以包含至少两种二元载体质粒的农杆菌菌株。每个二元载体包含在感兴趣的异源核苷酸序列侧翼的其自身的T-DNA边界。本发明的方法包括使用这些新型多元二元载体农杆菌菌株共同转化植物。以这种方式，驻留在不同二元载体上的感兴趣的异源核苷酸序列可以在单个转化事件中独立地引入植物中，并以非连接的方式并入植物的核DNA中。本发明还提供了再生的，可育的转基因植物，由其产生的转基因种子，以及T1和随后的几代。

16. 发明授权

US09574208B2 Methods and compositions for producing male sterile plants 有权
标题翻译：用于生产雄性不育植物的方法和组合物
公开(公告)号：US09574208B2
公开(公告)日：2017-02-21
申请号：US14129038
申请日：2012-06-19
申请人： Dennis L. Bidney , Mark Cigan , Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
发明人： Dennis L. Bidney , Mark Cigan , Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
IPC分类号： C12N15/82 , C12N5/14
CPC分类号： C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
摘要翻译：公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。包含其中具有至少一个靶向修饰的雄性能育基因的核酸分子，编码工程化双链断裂诱导剂和表达盒的内切核酸酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物进一步公开。

17. 发明授权

US06403861B1 Production of pathogen resistant plants 有权
公开(公告)号：US06403861B1
公开(公告)日：2002-06-11
申请号：US09524840
申请日：2000-03-14
申请人： Dennis L. Bidney , David G. Charne , Glenn S. Cole , Mark K. Mancl , Igor Falak , Katherine A. P. Nazarian , Christopher J. Scelonge
发明人： Dennis L. Bidney , David G. Charne , Glenn S. Cole , Mark K. Mancl , Igor Falak , Katherine A. P. Nazarian , Christopher J. Scelonge
IPC分类号： A01H500
CPC分类号： C12N9/0008 , C12N9/0006 , C12N9/88 , C12N15/8271 , C12N15/8282
摘要： Methods for increasing resistance in plants to pathogens by the expression of a hydrogen peroxide/reactive oxygen species producing enzyme or an oxalate degrading enzyme. The present invention relates to a method of producing a pathogen resistant hybrid plant by crossing the appropriate transgenic expressing a hydrogen peroxide/reactive oxygen species producing enzyme or an oxalate degrading enzyme with pathogen tolerant lines or inbreds obtained through conventional genetic manipulations, or by transformation of tolerant plants or plant tissues with a hydrogen peroxide/reactive oxygen species producing gene or by altering the expression of an endogenase hydrogen peroxide/reactive oxygen species producing gene. The synergistic effect of expression of a hydrogen peroxide/reactive oxygen species producing enzyme or an oxalate degrading enzyme in a tolerant background gives significant and unexpectedly high resistance to the pathogens.

18. 发明授权

US06303846B1 Gene encoding oxalate decarboxylase from aspergillus phoenices 有权
标题翻译：编码来自曲霉菌的草酸脱羧酶的基因
公开(公告)号：US06303846B1
公开(公告)日：2001-10-16
申请号：US09290202
申请日：1999-04-12
申请人： Christopher J. Scelonge , Dennis L. Bidney
发明人： Christopher J. Scelonge , Dennis L. Bidney
IPC分类号： C12N504
CPC分类号： C12N15/8279 , C12N9/88 , C12N15/8209 , C12N15/8242 , C12N15/8257 , C12N15/8271
摘要： A novel nucleic acid sequence encoding Aspergillus phoenices oxalate decarboxylase (APOXD) has been determined, as well as the encoded amino acid sequence. The gene and its encoded protein are useful in degrading oxalate, in diagnostic assays of oxalate, and as a selectable marker.
摘要翻译：已经确定了编码Aspergillus phoenices草酸脱羧酶（APOXD）的新型核酸序列以及编码的氨基酸序列。该基因及其编码的蛋白质可用于降解草酸盐，在草酸盐的诊断测定中作为选择标记。

19. 发明申请

US20150184194A1 METHODS AND COMPOSITIONS FOR PRODUCING MALE STERILE PLANTS 审中-公开
标题翻译：用于生产雄性不育植物的方法和组合物
公开(公告)号：US20150184194A1
公开(公告)日：2015-07-02
申请号：US14129038
申请日：2012-06-19
申请人： Dennis L. Bidney , Mark Cigan , Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
发明人： Dennis L. Bidney , Mark Cigan , Carl Falco , Huirong Gao , Derek Jantz , Mike Lassner , Keith Lowe , Leszek Aleksander Lyznik , James Jefferson Smith
IPC分类号： C12N15/82
CPC分类号： C12N15/8289 , C12N5/14 , C12N9/22 , C12N15/8213 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C12Q2600/158 , C12Y301/00
摘要： Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.
摘要翻译：公开了在植物基因组中对雄性能育基因进行靶向修饰的方法。所述方法包括使植物细胞与能够在雄性能育基因中的靶序列中诱导双链断裂的工程化双链断裂诱导剂接触，并鉴定包含靶序列改变的细胞。还公开了包含雄性育性基因改变的雄性育性基因的植物，植物细胞，植物部分和种子。包含其中具有至少一个靶向修饰的雄性能育基因的核酸分子，编码工程化双链断裂诱导剂和表达盒的内切核酸酶的优化核酸分子，宿主细胞和包含一种或多种核酸分子的植物进一步公开。

20. 发明授权

US06630615B1 Defense-related signaling genes and methods of use 失效
标题翻译：国防相关信号基因和使用方法
公开(公告)号：US06630615B1
公开(公告)日：2003-10-07
申请号：US09640419
申请日：2000-08-17
申请人： Dennis L. Bidney , Oswald R. Crasta , Xu Hu , Guihua Lu
发明人： Dennis L. Bidney , Oswald R. Crasta , Xu Hu , Guihua Lu
IPC分类号： C12N1509
CPC分类号： C12N15/8216 , C07K14/415 , C12N9/00 , C12N15/8222 , C12N15/8226 , C12N15/8238 , C12N15/8239 , C12N15/8279 , C12N15/8282 , C12N15/8286 , Y02A40/162
摘要： The invention relates to the genetic manipulation of plants, particularly to the expression of nucleotide sequences from defense-related signaling genes. Isolated nucleotide sequences encoding a neoxanthin cleavage enzyme, an amino acid permease, and a novel protein are provided. Also provided are isolated nucleotide sequences comprising promoters that drive expression in a plant in an inducible or tissue-preferred manner. The nucleotide sequences find use in increasing the resistance of plants to pathogens and other stresses, modifying ABA metabolism in plants, modifying amino acid transport and content in plants, and regulating gene expression in plants. Additionally provided are isolated proteins, and transformed plants and seeds thereof.
摘要翻译：本发明涉及植物的遗传操作，特别涉及来自防御相关信号基因的核苷酸序列的表达。提供了编码新黄素裂解酶，氨基酸通透酶和新型蛋白质的分离的核苷酸序列。还提供了分离的核苷酸序列，其包含以诱导型或组织优选方式驱动植物表达的启动子。核苷酸序列用于增加植物对病原体和其他胁迫的抗性，改变植物中的ABA代谢，修饰植物中的氨基酸转运和内容，以及调节植物中的基因表达。另外提供了分离的蛋白质，以及转化的植物和种子。

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