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11. 发明申请

US20130127989A1 Conversion of 2-Dimensional Image Data into 3-Dimensional Image Data 有权
标题翻译：将二维图像数据转换为三维图像数据
公开(公告)号：US20130127989A1
公开(公告)日：2013-05-23
申请号：US13302445
申请日：2011-11-22
申请人： Caifu Chen , Junhua Zhou
发明人： Caifu Chen , Junhua Zhou
IPC分类号： H04N13/00 , G06T15/00
CPC分类号： G06T7/579 , G06T7/11 , H04N13/261
摘要： Two dimensional data is converted into three dimensional picture data in a method that can provide a real time high quality display during conversion. Pixels of a frame of picture data are segmented to create pixel segments by applying a k-means algorithm. The k-means algorithm groups pixels based on closeness of a combined value that includes luma, chroma, and motion information. By balancing this information the algorithm collects pixels into groups that are assigned relative depths to turn the two-dimensional information into three-dimensional information for display. Another method includes determining a depth map for the different pixel segments by determining an amount of motion of one of the pixel segments between two frames of a video and scaling the three-dimensional depth of one of the pixel segments based on the amount of motion between the two frames.
摘要翻译：二维数据可以在转换期间提供实时高质量显示的方法中被转换为三维图像数据。分割图像数据帧的像素以通过应用k均值算法来创建像素段。 k-means算法基于包含亮度，色度和运动信息的组合值的接近度对像素进行分组。通过平衡该信息，算法将像素收集到被分配相对深度的组中，以将二维信息转换为三维信息以供显示。另一种方法包括：通过确定视频的两个帧之间的像素段之一的运动量并且基于所述像素段之一之间的运动量来缩放像素段之一的三维深度来确定不同像素段的深度图两帧。

12. 发明授权

US06887664B2 Asynchronous primed PCR 有权
公开(公告)号：US06887664B2
公开(公告)日：2005-05-03
申请号：US09875211
申请日：2001-06-05
申请人： Caifu Chen , Michael Egholm , Lawrence A. Haff
发明人： Caifu Chen , Michael Egholm , Lawrence A. Haff
IPC分类号： G01N33/53 , C12N15/09 , C12Q1/68 , G01N33/566 , C07H21/00 , C07H21/02 , C12P19/34
CPC分类号： C12Q1/686 , C12Q2561/101 , C12Q2527/113 , C12Q2527/107
摘要： An asynchronous thermal cycling protocol for nucleic acid amplification uses two primers with thermal melting temperatures different by about 10 to 30° C. After the higher melting primer has annealed and polymerase mediated extension, the uncopied, single-stranded target sequence may be hybridized and detected by a probe. DNA probes may be cleaved by the exonuclease activity of a polymerase. The probe may be a non-cleaving analog such as PNA. When a probe is labelled with a reporter dye and a quencher selected to undergo energy transfer, e.g. FRET, fluorescence from the reporter dye may be effectively quenched when the probe is unbound. Upon hybridization of the probe to complementary target or upon cleavage while bound to target, the reporter dye is no longer quenched, resulting in a detectable amount of fluorescence. The second, lower-melting primer may be annealed and extended to generate a double-stranded nucleic acid. Amplification may be monitored in real time, including each cycle, or at the end point. The asynchronous PCR thermal cycling protocol can generate a preponderance of the PCR amplicon in single-stranded form by repetition at the end of the protocol of annealing and extension of the higher melting primer.

13. 发明申请

US20090087858A1 Two-color Real-time/End-point Quantitation of MicroRNAs (miRNAs) 审中-公开
标题翻译：微RNA的两色实时/终点定量（miRNA）
公开(公告)号：US20090087858A1
公开(公告)日：2009-04-02
申请号：US12330460
申请日：2008-12-08
申请人： Andrew K. Finn , Caifu Chen
发明人： Andrew K. Finn , Caifu Chen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6851 , C12Q2565/1025 , C12Q2537/143 , C12Q2525/161 , C12Q2525/301
摘要： The present invention is directed to methods, reagents, kits, and compositions for detecting target polynucleotide sequences, especially small target polynucleotides such as miRNAs, between two samples. A pair of linker probes can be employed in two different reactions to query a particular species of target polynucleotide. A pair of detector probes, a single forward primer specific for the target polynucleotide, and a reverse primer can be employed in an amplification reaction to query the difference in expression level of the target polynucleotide between the two samples. In some embodiments a plurality of small miRNAs are queried with a plurality of linker probes. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions.
摘要翻译：本发明涉及用于在两个样品之间检测靶多核苷酸序列，特别是小目标多核苷酸如miRNA的方法，试剂，试剂盒和组合物。可以在两个不同的反应中使用一对接头探针来查询特定种类的靶多核苷酸。可以在扩增反应中使用一对检测器探针，针对靶多核苷酸特异性的单一正向引物和反向引物来查询两个样品之间的靶多核苷酸表达水平的差异。在一些实施方案中，使用多个接头探针查询多个小miRNA。然后可以在多个扩增反应中解码多个查询的miRNA。

14. 发明申请

US20070259356A1 Concatameric Ligation Products: Compositions, Methods and Kits for Same 审中-公开
公开(公告)号：US20070259356A1
公开(公告)日：2007-11-08
申请号：US11609767
申请日：2006-12-12
申请人： Caifu Chen , Kevin Hennessy , Kai Lao , Teodoro Paner , Vinod Mirchandani
发明人： Caifu Chen , Kevin Hennessy , Kai Lao , Teodoro Paner , Vinod Mirchandani
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6876 , C12Q1/6816 , C12Q2533/107 , C12Q2525/301
摘要： The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.

15. 发明申请

US20070099228A1 Concatameric Ligation Products: Compositions, Methods and Kits for Same 审中-公开
标题翻译：聚合物连接产品：组合物，方法和试剂盒相同
公开(公告)号：US20070099228A1
公开(公告)日：2007-05-03
申请号：US11609776
申请日：2006-12-12
申请人： Caifu Chen , Kevin Hennessy , Kai Lao , Teodoro Paner , Vinod Mirchandani
发明人： Caifu Chen , Kevin Hennessy , Kai Lao , Teodoro Paner , Vinod Mirchandani
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6876 , C12Q1/6816 , C12Q2533/107 , C12Q2525/301
摘要： The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.
摘要翻译：本教导涉及用于检测样品中的一种或多种靶多核苷酸序列的方法，组合物和试剂盒，以及用于形成连续连接产物的方法组合物和试剂盒。在本教导的一些实施方案中，寡核苷酸与互补靶多核苷酸杂交并连接在一起以形成连接产物。在本教导的一些实施方案中，可以扩增连续连接产物，并且基于在连接反应中引入的序列确定靶多核苷酸的身份和数量。本教导的一些实施方案提供从反应混合物中除去未螯合的探针的方法。本教导的一些实施方案使用各种分析程序提供多重检测，鉴定和定量多个靶多核苷酸。

16. 发明申请

US20060063163A1 Concatameric ligation products: compositions, methods and kits for same 有权
标题翻译：聚合物连接产物：组合物，方法和试剂盒相同
公开(公告)号：US20060063163A1
公开(公告)日：2006-03-23
申请号：US10982619
申请日：2004-11-04
申请人： Caifu Chen , Kevin Hennessy , Kai Lao , Teodoro Paner , Vinod Mirchandani
发明人： Caifu Chen , Kevin Hennessy , Kai Lao , Teodoro Paner , Vinod Mirchandani
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12P19/34 , C12Q1/6813 , C12Q1/6816 , C12Q2600/156 , C12Q2525/125 , C12Q2521/501 , C12Q2521/319 , C12Q2525/301 , C12Q2565/125 , C12Q2533/107
摘要： The present teachings relate to methods, compositions, and kits for detecting one or more target polynucleotide sequences in a sample, and methods compositions and kits for forming concatameric ligation products. In some embodiments of the present teachings, oligonucleotides are hybridized to complementary target polynucleotides and are ligated together to form a concatameric ligation product. In some embodiments of the present teachings, the concatameric ligation product can be amplified, and the identity and quantity of the target polynucleotides determined based on sequence introduced in the ligation reaction. Some embodiments of the present teachings provide methods for removing unligated probes from the reaction mixture. Some embodiments of the present teachings provide for highly multiplexed detection, identification, and quantification of a plurality of target polynucleotides using a variety of analytical procedures.
摘要翻译：本教导涉及用于检测样品中的一种或多种靶多核苷酸序列的方法，组合物和试剂盒，以及用于形成连续连接产物的方法组合物和试剂盒。在本教导的一些实施方案中，寡核苷酸与互补靶多核苷酸杂交并连接在一起以形成连接产物。在本教导的一些实施方案中，可以扩增连续连接产物，并且基于在连接反应中引入的序列确定靶多核苷酸的身份和数量。本教导的一些实施方案提供从反应混合物中除去未螯合的探针的方法。本教导的一些实施方案使用各种分析程序提供多重检测，鉴定和定量多个靶多核苷酸。

17. 发明授权

US06297016B1 Template-dependent ligation with PNA-DNA chimeric probes 有权
标题翻译：与PNA-DNA嵌合探针的模板依赖性连接
公开(公告)号：US06297016B1
公开(公告)日：2001-10-02
申请号：US09416003
申请日：1999-10-08
申请人： Michael Egholm , Caifu Chen
发明人： Michael Egholm , Caifu Chen
IPC分类号： C12Q168
CPC分类号： C12Q1/6862
摘要： The invention provides methods, kits, and compositions for ligation of PNA-DNA chimeric probes and oligonucleotides when they are hybridized adjacently to template nucleic acids using ligases and ligation reagents. Structural requirements of the chimeras for ligation include 5 to 15 contiguous PNA monomer units, 2 or more contiguous nucleotides, and a 3′ hydroxyl or 5′ hydroxyl terminus. The chimera and/or oligonucleotide may be labelled with fluorescent dyes or other labels. The methods include, for example, oligonucleotide-ligation assays (OLA) and single nucleotide polymorphism detection.
摘要翻译：当使用连接酶和连接试剂将它们与模板核酸相互杂交时，本发明提供用于连接PNA-DNA嵌合探针和寡核苷酸的方法，试剂盒和组合物。用于连接的嵌合体的结构要求包括5至15个连续的PNA单体单元，2个或更多个连续核苷酸，以及3'羟基或5'羟基末端。嵌合体和/或寡核苷酸可以用荧光染料或其他标记物标记。所述方法包括例如寡核苷酸连接测定（OLA）和单核苷酸多态性检测。

18. 发明授权

US09303281B2 Compositions for detecting foodstuff spoilage microorganisms 有权
标题翻译：用于检测食品腐败微生物的组合物
公开(公告)号：US09303281B2
公开(公告)日：2016-04-05
申请号：US13555399
申请日：2012-07-23
申请人： Caifu Chen
发明人： Caifu Chen
IPC分类号： C12Q1/68 , C12Q1/04 , C12Q1/26 , C12Q1/58 , C12Q1/66
CPC分类号： C12Q1/04 , C12Q1/26 , C12Q1/58 , C12Q1/66 , C12Q1/689
摘要： The invention provides nucleic acids, collections of nucleic acids, assay kits, and methods for the sensitive and specific detection of microorganisms in a foodstuff. The nucleic acid consists of a nucleotide sequence selected from the group consisting of SEQ ID NOs: 1-45.
摘要翻译：本发明提供核酸，核酸的集合，测定试剂盒以及用于敏感和特异性检测食品中的微生物的方法。核酸由选自SEQ ID NO：1-45的核苷酸序列组成。

19. 发明授权

US09041773B2 Conversion of 2-dimensional image data into 3-dimensional image data 有权
标题翻译：将二维图像数据转换为三维图像数据
公开(公告)号：US09041773B2
公开(公告)日：2015-05-26
申请号：US13302445
申请日：2011-11-22
申请人： Caifu Chen , Junhua Zhou
发明人： Caifu Chen , Junhua Zhou
IPC分类号： H04N13/00 , H04N13/02 , G06T7/00
CPC分类号： G06T7/579 , G06T7/11 , H04N13/261
摘要： Two dimensional data is converted into three dimensional picture data in a method that can provide a real time high quality display during conversion. Pixels of a frame of picture data are segmented to create pixel segments by applying a k-means algorithm. The k-means algorithm groups pixels based on closeness of a combined value that includes luma, chroma, and motion information. By balancing this information the algorithm collects pixels into groups that are assigned relative depths to turn the two-dimensional information into three-dimensional information for display. Another method includes determining a depth map for the different pixel segments by determining an amount of motion of one of the pixel segments between two frames of a video and scaling the three-dimensional depth of one of the pixel segments based on the amount of motion between the two frames.
摘要翻译：二维数据可以在转换期间提供实时高质量显示的方法中被转换为三维图像数据。分割图像数据帧的像素以通过应用k均值算法来创建像素段。 k-means算法基于包含亮度，色度和运动信息的组合值的接近度对像素进行分组。通过平衡该信息，算法将像素收集到被分配相对深度的组中，以将二维信息转换为三维信息以供显示。另一种方法包括：通过确定视频的两个帧之间的像素段之一的运动量并且基于所述像素段之一之间的运动量来缩放像素段之一的三维深度来确定不同像素段的深度图两帧。

20. 发明授权

US08187815B2 Method to quantify siRNAs, miRNAs and polymorphic miRNAs 有权
标题翻译：定量siRNA，miRNA和多态性miRNA的方法
公开(公告)号：US08187815B2
公开(公告)日：2012-05-29
申请号：US13015332
申请日：2011-01-27
申请人： Ruoying Tan , Caifu Chen , Karl J. Guegler
发明人： Ruoying Tan , Caifu Chen , Karl J. Guegler
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q1/6851 , C12Q2525/301 , C12Q2525/207 , C12Q2521/501 , C12Q2537/143
摘要： The present teachings provide methods, compositions, and kits for quantifying target polynucleotides. In some embodiments, a reverse stem-loop ligation probe is ligated to the 3′ end of a target polynucleotide, using a ligase that can ligate the 3′ end of RNA to the 5′ end of DNA using a DNA template, such as T4 DNA ligase. Following digestion to form an elongated target polynucleotide with a liberated end, a reverse transcription reaction can be performed, followed by a PCR. In some embodiments, the methods of the present teachings can discriminate between polymorphic polynucleotides that vary by as little as one nucleotide.
摘要翻译：本教导提供了用于定量靶多核苷酸的方法，组合物和试剂盒。在一些实施方案中，使用连接酶将反向茎环结合探针连接到靶多核苷酸的3'末端，该连接酶可以使用DNA模板如T4连接DNA的3'末端至DNA的5'末端 DNA连接酶。在消化形成具有释放末端的细长靶多核苷酸之后，可以进行逆转录反应，随后进行PCR。在一些实施方案中，本教导的方法可以区分多至一个核苷酸变化的多态性多核苷酸。

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